Method for processing preserved szechuan pickle waste water by compound bacterial flora

A technology of compound flora and mustard wastewater is applied in the field of environmental microorganisms, which can solve the problems of affecting the living environment of surrounding residents, unfavorable large-scale treatment of wastewater, and difficulty in mustard wastewater, and achieves good sludge settling performance and good phosphorus and nitrogen removal effects. , good treatment effect

Inactive Publication Date: 2010-01-20
ZHEJIANG UNIV
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Problems solved by technology

[0003] However, the development of the pickled mustard industry has also brought serious environmental pollution problems to the local area; mustard mustard production will generate a large amount of wastewater, including wastewater discharged from production processes such as pickling, washing, and desalination, which has a high salt concentration and Organic concentration
The direct discharge of mustard wastewater will cause serious environmental pollution: first, it will lead to eutrophication of the receiving water system, causing the water body to turn black and smelly; The waste water itself emits an unpleasant odor, which seriously affe

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  • Method for processing preserved szechuan pickle waste water by compound bacterial flora

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Embodiment 1

[0029] Embodiment 1, the isolation of bacterial strain in seawater and saline lake water sample

[0030]Mix the bottled water sample evenly, and filter it with a filter (sterile) equipped with a 0.22 μm microporous membrane under sterile conditions, and enrich the bacteria on the filter; the amount of enriched water sample reaches 20-30ml , carefully remove the filter membrane, put it into a 3ml liquid medium test tube, and oscillate to separate the bacteria from the filter membrane; after mixing, make an appropriate gradient dilution, draw 100 μl of liquid onto the 2216 medium plate, and spread evenly, each gradient Spread 2 plates and culture them statically at 25°C and 37°C respectively. Randomly select a single colony, streak the plate repeatedly until the color, size and shape of the colonies on the plate are completely consistent, and record the morphological characteristics of the colony. After identification, Halomonas and Seabacteria listed in Table 1 were obtained. ...

Embodiment 2

[0031] Embodiment 2, the isolation of bacterial strain in the water sample of mustard mustard factory sewage discharge outlet

[0032] Mix the bottled water sample and dilute it with mustard mustard wastewater with pH 7.0 and filter sterilization; select the dilution factor as 10 4 and 10 6 Draw 100 μl of liquid onto the medium plate of mustard mustard wastewater (adjust the pH to 7.0 by adding 2% agar, and sterilize at 121°C for 30 minutes), and coat evenly. Each gradient is coated with 2 Plates were cultured statically at 25°C and 37°C, respectively. Randomly select a single colony, streak the plate repeatedly until the color, size and shape of the colonies on the plate are completely consistent, and record the morphological characteristics of the colony. After identification, the Bacillus and Lemonococcus listed in Table 1 were obtained.

Embodiment 3

[0033] Embodiment 3, the isolation of bacterial strain in the sludge sample of mustard vegetable factory sewage discharge outlet

[0034] Put 2g of the mud sample collected from the sewage discharge outlet of Cixi mustard plant in Zhejiang into 50ml of filtered mustard wastewater, add glass beads to oscillate and break up, take the supernatant and dilute it with pH 7.0 and filter-sterilized mustard wastewater; choose the dilution factor for 10 4 and 10 6 Draw 100 μl of liquid onto the medium plate of mustard mustard wastewater (adjust the pH to 7.0 by adding 2% agar, and sterilize at 121°C for 30 minutes), and coat evenly. Each gradient is coated with 2 Plates were cultured statically at 25°C and 37°C, respectively. Randomly select a single colony, streak the plate repeatedly until the color, size and shape of the colonies on the plate are completely consistent, and record the morphological characteristics of the colony. After identification, the Candida bacteria listed in ...

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Abstract

The invention discloses a method for processing preserved szechuan pickle waste water by halophilic (haloduric) bacteria, which uses a compound bacterial flora mainly including microzyme, marinobacter and bacillus and combines an SBR technology to process the preserved szechuan pickle waste water so as to realize COD removing rate more than 90 percent within 24 hours. The method for processing the preserved szechuan pickle waste water has simple technology, stable water outflow and good load impact resistance and can realize the high-efficiency processing of the processing preserved szechuan pickle waste water with lower cost.

Description

technical field [0001] The invention belongs to the technical field of environmental microbes, and in particular relates to a composite flora of halophilic (tolerant) bacteria and its use in the treatment of pickled mustard wastewater. Background technique [0002] Pickled mustard is a semi-dry non-fermented pickle, which is pickled with mustard greens as raw materials. It is one of the famous special products in China. It is one of the three famous pickles in the world together with German sweet and sour cabbage and European pickled cucumber. A kind of seasoning dish that our people like. Mustard mustard industry is also a pillar industry of the rural economy in Fuling, Chongqing, Yuyao, Zhejiang and other places. [0003] However, the development of the pickled mustard industry has also brought serious environmental pollution problems to the local area; mustard mustard production will generate a large amount of wastewater, including wastewater discharged from production p...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C02F3/12C02F3/34C12R1/72C12R1/07C12R1/01C02F103/26
CPCY02W10/10
Inventor 吴敏许学伟孟凡旭张勇
Owner ZHEJIANG UNIV
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