Serum-free NK cell culture medium
By using interleukin-type NK cell activating factors, fat-soluble vitamins, trace elements, and serum substitutes as activators and amplifying agents, combined with coating agents such as anti-CD38 antibodies, a serum-free culture medium and culture vessel were constructed. This solved the safety risks and high costs associated with serum use in existing NK cell cultures, and achieved efficient amplification and activation of NK cells.
CN118667759BActive Publication Date: 2026-01-06HANGZHOU BIOGNK BIOTECHNOLOGY CO LTD
6 Cites 0 Cited by
Patent Information
- Application Number
- CN202410800264.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-20
- Publication Date
- 2026-01-06
- Estimated Expiration
- 2044-06-20
AI Technical Summary
Technical Problem
Existing human NK cell culture methods require the addition of human serum or autologous human plasma, which poses safety risks and high costs, and there is a lack of effective serum-free culture medium alternatives.
Method used
Serum-free culture medium and culture vessel were constructed using activators and amplifying agents containing interleukin-type NK cell activating factors, fat-soluble vitamins, trace elements and serum substitutes, combined with coating agents such as anti-CD38 antibody, to achieve serum-free culture of NK cells.
Benefits of technology
It achieves efficient expansion and activation of NK cells, avoids the safety risks associated with serum, reduces costs, and improves the killing ability and quantity of NK cells.
✦ Generated by Eureka AI based on patent content.
Abstract
Disclosed herein are activators and expanders for NK cell culture, coating agents for NK cell culture vessels, serum-free media for activation and expansion steps of NK cell culture, and methods of NK cell culture.
Need to check novelty before this filing date? Find Prior Art