A herbal hair loss prevention, hair growth and hair darkening composition and preparation method thereof

Abstract

The present invention relates to the technical field of cosmetics, and in particular to a herbal hair loss prevention, hair growth, and hair darkening composition and a preparation method thereof. The present invention adopts Polygonum multiflorum, Elm bark, ginger, alfalfa, Gallnut, Lycium bark, and Hamamelis virginiana, which have hair loss prevention, hair growth, and hair darkening effects, to prepare a hair composition. The present invention optimizes the ratio of raw materials, enhances the matching effect of active ingredients, and makes the hair loss prevention, hair growth, and hair darkening effects of the composition better. At the same time, the present invention adopts hot water extraction and dissolution, composite bacteria combined fermentation, and ethanol aqueous solution extraction processes to increase the types of active ingredients and the concentration of active substances, enhance the efficacy of the medicine, and can further improve the hair loss prevention, hair growth, and hair darkening effects of the composition.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, and in particular to a herbal hair loss prevention, hair growth and hair darkening composition and a preparation method thereof. Background Art

[0002] Normally shed hair is hair that is in the regression phase and the resting phase. When the hair that has entered the regression phase and the hair that has just entered the growth phase are in a dynamic balance, a normal amount of hair can be maintained. When this dynamic balance is broken, overall hair loss will occur.

[0003] There are abundant blood vessels in the hair papilla of human hair, which provide sufficient nutrition for the hair papilla and hair bulb, so that melanin particles can be synthesized smoothly. However, various adverse stimuli can cause spasms in the blood vessels that supply nutrition to the hair, which can cause the melanocytes in the hair papilla and hair bulb to secrete melanin, affecting the formation and transportation of melanin particles, causing the hair to gradually turn white.

[0004] The quality of some cosmetics currently used to relieve hair loss, promote hair growth or darken hair varies greatly, and more chemical preparations are used, resulting in relatively large side effects. Therefore, providing a herbal anti-hair loss, hair growth and darkening hair composition and a preparation method thereof is one of the technical problems that need to be solved at present. Summary of the invention

[0005] Based on the solution of the above technical problems, the technical solution of the present invention is proposed. The technical solution of the present invention includes a herbal hair loss prevention, hair growth and hair darkening composition and a preparation method.

[0006] The present invention adopts Polygonum multiflorum, Elm bark, ginger, alfalfa, Chinese gallnut, wolfberry and Hamamelis virginiana which have the effects of preventing hair loss, promoting hair growth and darkening hair to prepare a composition. The ratio of raw materials is optimized, the matching effect of active ingredients is optimized, and the effect of the composition is enhanced. At the same time, the present invention adopts hot water extraction and dissolution, composite bacteria combined fermentation and ethanol aqueous solution extraction and other processes to increase the types and concentrations of active ingredients, enhance the efficacy of the medicine, and further increase the effects of the composition of preventing hair loss, promoting hair growth and darkening hair.

[0007] In one aspect, the present invention provides a method for preparing a herbal hair loss prevention, hair growth and hair darkening composition, comprising the following preparation steps:

[0008] Step 1: Weigh the following dried herbal plants according to weight: 0.5-2 parts of Polygonum multiflorum, 0.4-1.8 parts of Elm bark, 0.5-1.5 parts of ginger, 1-3 parts of alfalfa, 0.5-2.2 parts of Galla chinensis, 0.1-0.8 parts of Lycium barbarum, and 0.1-0.6 parts of Hamamelis virginiana;

[0009] Step 2: Mix the herbal plants and crush them to obtain a mixed material; mix the mixed material with water at a mass ratio of 1:15-100, heat to 40-65° C. and stir for 5-15 hours to obtain material A;

[0010] Step 3: sterilize material A, cool it, inoculate it with fermentation bacteria to obtain material B, and perform anaerobically fermentation at 28-40°C for 10-30 hours to obtain a fermented material;

[0011] Step 4: sterilize the fermentation material, add ethanol, adjust the ethanol mass concentration to 32-55%, heat and stir to obtain material C;

[0012] Step 5: Filter material C to obtain a filtrate, and concentrate, dry, and sterilize the filtrate to obtain the composition.

[0013] Furthermore, in step 3, the fermentation bacteria are Lactobacillus reuteri CICC 6121 and Lactobacillus acidophilus CICC6081.

[0014] Further, in step 3, the fermentation bacteria content in material B is 3×10 7 -9×10 7 cfu / mL;

[0015] Furthermore, in step 3, in material B, the ratio of the number of live bacteria of Lactobacillus reuteri CICC 6121 and Lactobacillus acidophilus CICC6081 is 0.5-1:1.

[0016] Furthermore, in step 1, the mass ratio of elm bark to alfalfa is 1:2-4.

[0017] Furthermore, in step 1, the amount of herbal plants used is: 0.6-1.8 parts of Polygonum multiflorum, 0.5-1.5 parts of elm bark, 0.6-1.2 parts of ginger, 1.2-2.8 parts of alfalfa, 0.6-2 parts of gallnut, 0.2-0.6 parts of wolfberry and 0.2-0.5 parts of witch hazel.

[0018] Furthermore, in step 2, the herbal plant is crushed to a particle size not greater than 0.1 mm.

[0019] Furthermore, in step 2, the mass ratio of the mixed material to water is 1:20-80.

[0020] Furthermore, in step 2, the mixture is heated to 55-62° C. and stirred for 8-12 hours.

[0021] Furthermore, in step 3, cooling to room temperature is performed.

[0022] Further, in step 3, anaerobic fermentation is performed at 32-40° C. for 12-18 hours.

[0023] Furthermore, in step 3, fermentation is carried out with stirring, and the stirring speed is 50-120 rpm.

[0024] Furthermore, in step 4, the ethanol mass concentration is adjusted to 35-50%.

[0025] Furthermore, in step 4, the heating and stirring is heating to 42-60° C. and stirring for 4-10 hours.

[0026] Furthermore, in step 5, the filtration is performed by cooling material C to 25-32°C.

[0027] Furthermore, in step 5, filtration is performed using a filter membrane with a pore size of 0.1-5 μm.

[0028] Furthermore, in step 5, the concentration is reduced-pressure concentration, the concentration temperature is 58-62° C., and the concentration pressure is 4 KPa-8 KPa.

[0029] Further, in step 5, the concentration is performed to a mass of 8-15% of the mass of the filtrate.

[0030] Furthermore, in step 5, the drying is freeze-drying.

[0031] Furthermore, in step 5, the freeze-drying temperature is -40°C to -28°C.

[0032] Furthermore, in step 5, the mixture is dried until the water content by mass is less than 8.2%.

[0033] On the other hand, the present invention also provides a herbal hair loss prevention, hair growth and hair darkening composition prepared by the preparation method.

[0034] On the other hand, the present invention also provides use of the composition in preparing cosmetics.

[0035] Furthermore, the cosmetic is at least one of essence, cream, ointment and powder.

[0036] The beneficial effects of the present invention are as follows:

[0037] The present invention adopts Polygonum multiflorum, Elm bark, ginger, alfalfa, Chinese nut, Chinese wolfberry and Hamamelis virginiana which have the effects of preventing hair loss, promoting hair growth and darkening hair to prepare a hair composition. The present invention optimizes the ratio of raw materials, enhances the matching effect of active ingredients, and makes the composition have better effects of preventing hair loss, promoting hair growth and darkening hair. At the same time, the present invention adopts processes such as hot water extraction and dissolution, composite bacteria combined fermentation and ethanol aqueous solution extraction to increase the types of active ingredients and the concentration of active substances, enhance the performance of drug efficacy, and can further improve the effects of the composition of preventing hair loss, promoting hair growth and darkening hair.

[0038] The composition of the present invention caused the skin of the depilated mice to turn from pink to black on the 7.213±0.283th day, and caused the skin of the depilated area to start growing new hair on the 10.583±0.614th day; compared with the model group, the composition of the present invention caused the time for the mouse skin to turn from pink to black to be advanced by 1.741 days, and caused the time for the mouse skin to grow hair to be advanced by 1.993 days. The composition of the present invention has a good effect of preventing hair loss and promoting hair growth.

[0039] The fermentation process used in the preparation method of the present invention can significantly improve the effect of the composition in promoting hair growth. The fermentation bacteria selected in the present invention make the composition prepared by the present invention more effective than other fermentation bacteria. The present invention uses Lactobacillus mucilaginosus CICC 6121 and Lactobacillus acidophilus CICC 6081 for co-fermentation, which increases the concentration and activity of the hair growth active ingredients of the herbal plants, and the two synergistically promote the combined hair growth effect; and the ratio of the number of fermentation bacteria of the two also affects the effect of the composition.

[0040] In the preparation system of the present invention, the combination and dosage ratio of the preparation raw materials elm bark and alfalfa, as well as the concentration of ethanol used in the extraction process, all have an impact on the hair growth effect of the composition; in the present invention, the dosage ratio of elm bark and alfalfa is 1:2-4 and the ethanol concentration during extraction is 32-55%, and the hair growth effect of the obtained composition is better.

[0041] The composition prepared by the present invention can effectively promote the activation of tyrosinase and has a good hair darkening effect. The tyrosinase activation rate test of the present invention shows that the composition of the present invention makes the tyrosinase activation rate reach 187.43%.

[0042] The composition prepared by the present invention has low irritation, and a skin irritation test shows that the irritation level is level 0. DETAILED DESCRIPTION

[0043] The present invention will be described below in conjunction with specific embodiments, and various effects of the present invention will be more clearly presented. It should be understood by those skilled in the art that these specific embodiments are used to illustrate the present invention, rather than to limit the present invention.

[0044] The present invention relates to a method for preparing a herbal hair loss prevention, hair growth and hair darkening composition, comprising the following preparation steps:

[0045] Step 1: Weigh the following dried herbal plants according to mass: 0.5-2 parts of Polygonum multiflorum, 0.4-1.8 parts of Ulmus pumila bark, 0.5-1.5 parts of ginger, 1-3 parts of alfalfa, 0.5-2.2 parts of Galla chinensis, 0.1-0.8 parts of Lycium barbarum and 0.1-0.6 parts of Hamamelis virginiana; wherein the mass ratio of the Ulmus pumila bark to the alfalfa is 1:2-4;

[0046] Step 2: Mix the herbal plants and crush them to obtain a mixed material; mix the mixed material with water at a ratio of 1:15-100, heat to 40-65° C. and stir for 5-15 hours to obtain material A;

[0047] Step 3: sterilize material A, cool it, inoculate fermentation bacteria to obtain material B, and perform anaerobically fermentation at 28-40°C for 10-30 hours to obtain a fermented material; wherein the fermentation bacteria are Lactobacillus reuteri CICC 6121 and Lactobacillus acidophilus CICC 6081; the fermentation bacteria content is 3×10 7 -9×10 7 cfu / mL; the ratio of the number of live bacteria of Lactobacillus reuteri CICC 6121 and Lactobacillus acidophilus CICC 6081 is 0.5-1:1; the fermentation is carried out with stirring, and the stirring speed is 50-120rpm;

[0048] Step 4: sterilize the fermentation material, add ethanol, adjust the ethanol mass concentration to 32-55%, heat to 42-60°C and stir for 4-10 hours to obtain material C;

[0049] Step 5: Filter material C to obtain a filtrate, concentrate the filtrate under reduced pressure, freeze-dry until the water content is less than 8.2% by mass, and sterilize to obtain the composition.

[0050] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0051] Lactobacillus reuteri CICC 6121: Limosilactobacillus reuteri , CICC®6121, purchased from China Industrial Microbiological Culture Collection Center.

[0052] Lactobacillus acidophilus CICC 6081: Lactobacillus acidophilus , CICC®6081, purchased from China Industrial Microbiological Culture Collection Center.

[0053] Lactobacillus rhamnosus ATCC 53103: Lactobacillus rhamnosus , No.: ATCC 53103, purchased from the GBW Information Network, www.gbw.org.cn.

[0054] 1. Preparation of the composition

[0055] 1. Composition 1

[0056] The preparation steps of the composition are as follows:

[0057] Step 1: Weigh the following dried herbal plants according to their mass: 1.2 parts of Polygonum multiflorum, 0.9 parts of Ulmus pumila bark, 0.8 parts of ginger, 2.7 parts of alfalfa, 0.8 parts of Galla chinensis, 0.4 parts of Lycium barbarum and 0.5 parts of Hamamelis virginiana; wherein the mass ratio of the Ulmus pumila bark to the alfalfa is 1:3;

[0058] Step 2: Mix the herbal plants and crush them until the particle size of the herbal plants is no more than 0.1 mm to obtain a mixed material; mix the mixed material with water at a ratio of 1:45, heat to 55° C. and stir for 8 hours to obtain material A;

[0059] Step 3: sterilize material A with ultraviolet light, cool it to room temperature, inoculate fermentation bacteria to obtain material B, and perform anaerobic fermentation at 35°C and 55rpm for 22 hours to obtain a fermented material;

[0060] Among them, the fermentation bacteria are Lactobacillus reuteri CICC 6121 and Lactobacillus acidophilus CICC 6081; in material B, the fermentation bacteria content is 7.2×10 7 cfu / mL, the ratio of viable counts of Lactobacillus reuteri CICC 6121 and Lactobacillus acidophilus CICC 6081 was 0.8:1;

[0061] Step 4: sterilize the fermentation material, add ethanol to adjust the ethanol mass concentration to 43%, heat to 50°C, stir at 80rpm for 5 hours, and obtain material C;

[0062] Step 5: Cool material C to 30° C., filter using a filter membrane with a pore size of 0.22 μm to obtain a filtrate, and concentrate the filtrate under reduced pressure at a temperature of 60° C. and a pressure of 5.8 KPa to a mass of 12% of the mass of the filtrate to obtain a concentrate;

[0063] The concentrate is freeze-dried at a temperature of -35°C to a water content of about 5.7% by weight; and then sterilized by ultraviolet light to obtain the composition 1.

[0064] 2. Composition 2

[0065] The preparation steps of the composition are as follows:

[0066] Step 1: Weigh the following dried herbal plants according to mass: 1 part of Polygonum multiflorum, 1.2 parts of Ulmus pumila bark, 1.2 parts of ginger, 2.4 parts of alfalfa, 1.3 parts of Galla chinensis, 0.25 parts of Lycium barbarum and 0.35 parts of Hamamelis virginiana; wherein the mass ratio of the Ulmus pumila bark to the alfalfa is 1:2;

[0067] Step 2: Mix the herbal plants and crush them until the particle size of the herbal plants is no more than 0.1 mm to obtain a mixed material; mix the mixed material with water at a ratio of 1:48, heat to 52° C. and stir for 9 hours to obtain material A;

[0068] Step 3: sterilize material A with ultraviolet light, cool it to room temperature, inoculate fermentation bacteria to obtain material B, and perform anaerobic fermentation at 32° C. and 50 rpm for 25 hours to obtain a fermented material;

[0069] Among them, the fermentation bacteria are Lactobacillus reuteri CICC 6121 and Lactobacillus acidophilus CICC 6081; in material B, the fermentation bacteria content is 7.5×10 7 cfu / mL, the ratio of viable counts of Lactobacillus reuteri CICC 6121 and Lactobacillus acidophilus CICC 6081 was 1:1;

[0070] Step 4: sterilize the fermentation material, add ethanol to adjust the ethanol mass concentration to 35%, heat to 52°C, stir at 70rpm for 6.2 hours, and obtain material C;

[0071] Step 5: Cool material C to 28° C., filter using a filter membrane with a pore size of 0.22 μm to obtain a filtrate, and concentrate the filtrate under reduced pressure at a temperature of 60° C. and a pressure of 5.8 KPa to a mass of 11.5% of the mass of the filtrate to obtain a concentrate;

[0072] The concentrate is freeze-dried at -35°C to a water content of about 5.8% by mass; and then sterilized by ultraviolet light to obtain the composition 2.

[0073] 3. Composition 3

[0074] The preparation steps of the composition are as follows:

[0075] Step 1: Weigh the following dried herbal plants according to their mass: 1.2 parts of Polygonum multiflorum, 0.9 parts of Ulmus pumila bark, 0.8 parts of ginger, 2.7 parts of alfalfa, 0.8 parts of Galla chinensis, 0.4 parts of Lycium barbarum and 0.5 parts of Hamamelis virginiana; wherein the mass ratio of the Ulmus pumila bark to the alfalfa is 1:3;

[0076] Step 2: Mix the herbal plants and crush them until the particle size of the herbal plants is no more than 0.1 mm to obtain a mixed material; mix the mixed material with water at a ratio of 1:45, heat to 55° C. and stir for 8 hours to obtain material A;

[0077] Step 3: sterilize material A with ultraviolet light, cool it to room temperature, inoculate fermentation bacteria to obtain material B, and perform anaerobic fermentation at 35°C and 55rpm for 22 hours to obtain a fermented material;

[0078] Among them, the fermentation bacteria is Lactobacillus rhamnosus ATCC 53103; in material B, the fermentation bacteria content is 7.2×10 7 cfu / mL;

[0079] Step 4: sterilize the fermentation material, add ethanol to adjust the ethanol mass concentration to 43%, heat to 50°C, stir at 80rpm for 5 hours, and obtain material C;

[0080] Step 5: Cool material C to 30° C., filter using a filter membrane with a pore size of 0.22 μm to obtain a filtrate, and concentrate the filtrate under reduced pressure at a temperature of 60° C. and a pressure of 5.8 KPa to a mass of 12% of the mass of the filtrate to obtain a concentrate;

[0081] The concentrate is freeze-dried at -35°C to a water content of about 5.7% by mass; and then sterilized by ultraviolet light to obtain the composition 3.

[0082] 4. Composition 4

[0083] The preparation steps of the composition are as follows:

[0084] Step 1: Weigh the following dried herbal plants according to their mass: 1.2 parts of Polygonum multiflorum, 0.9 parts of Ulmus pumila bark, 0.8 parts of ginger, 2.7 parts of alfalfa, 0.8 parts of Galla chinensis, 0.4 parts of Lycium barbarum and 0.5 parts of Hamamelis virginiana; wherein the mass ratio of the Ulmus pumila bark to the alfalfa is 1:3;

[0085] Step 2: Mix the herbal plants and crush them until the particle size of the herbal plants is no more than 0.1 mm to obtain a mixed material; mix the mixed material with water at a ratio of 1:45, heat to 55° C. and stir for 8 hours to obtain material A;

[0086] Step 3: sterilize material A with ultraviolet light, cool it to room temperature, inoculate fermentation bacteria to obtain material B, and perform anaerobic fermentation at 35°C and 55rpm for 22 hours to obtain a fermented material;

[0087] Among them, the fermentation bacteria are Lactobacillus reuteri CICC 6121 and Lactobacillus rhamnosus ATCC 53103; in material B, the fermentation bacteria content is 7.2×10 7 cfu / mL, the ratio of viable bacteria count of Lactobacillus reuteri CICC 6121 and Lactobacillus rhamnosus ATCC53103 was 0.8:1;

[0088] Step 4: sterilize the fermentation material, add ethanol to adjust the ethanol mass concentration to 43%, heat to 50°C, stir at 80rpm for 5 hours, and obtain material C;

[0089] Step 5: Cool material C to 30° C., filter using a filter membrane with a pore size of 0.22 μm to obtain a filtrate, and concentrate the filtrate under reduced pressure at a temperature of 60° C. and a pressure of 5.8 KPa to a mass of 12% of the mass of the filtrate to obtain a concentrate;

[0090] The concentrate is freeze-dried at -35°C to a water content of about 5.7% by mass; and then sterilized by ultraviolet light to obtain the composition 4.

[0091] 5. Composition 5

[0092] The preparation steps of the composition are as follows:

[0093] Step 1: Weigh the following dried herbal plants according to their mass: 1.2 parts of Polygonum multiflorum, 0.9 parts of Ulmus pumila bark, 0.8 parts of ginger, 2.7 parts of alfalfa, 0.8 parts of Galla chinensis, 0.4 parts of Lycium barbarum and 0.5 parts of Hamamelis virginiana; wherein the mass ratio of the Ulmus pumila bark to the alfalfa is 1:3;

[0094] Step 2: Mix the herbal plants and crush them until the particle size of the herbal plants is no more than 0.1 mm to obtain a mixed material; mix the mixed material with water at a ratio of 1:45, heat to 55° C. and stir for 8 hours to obtain material A;

[0095] Step 3: sterilize material A with ultraviolet light, cool it to room temperature, inoculate fermentation bacteria to obtain material B, and perform anaerobic fermentation at 35°C and 55rpm for 22 hours to obtain a fermented material;

[0096] The fermentation bacteria is Lactobacillus acidophilus CICC 6081; the fermentation bacteria content in material B is 7.2×10 7 cfu / mL;

[0097] Step 4: sterilize the fermentation material, add ethanol to adjust the ethanol mass concentration to 43%, heat to 50°C, stir at 80rpm for 5 hours, and obtain material C;

[0098] Step 5: Cool material C to 30° C., filter using a filter membrane with a pore size of 0.22 μm to obtain a filtrate, and concentrate the filtrate under reduced pressure at a temperature of 60° C. and a pressure of 5.8 KPa to a mass of 12% of the mass of the filtrate to obtain a concentrate;

[0099] The concentrate is freeze-dried at -35°C to a water content of about 5.7% by mass; and then sterilized by ultraviolet light to obtain the composition 5.

[0100] 6. Composition 6

[0101] The preparation steps of the composition are as follows:

[0102] Step 1: Weigh the following dried herbal plants according to their mass: 1.2 parts of Polygonum multiflorum, 0.9 parts of Ulmus pumila bark, 0.8 parts of ginger, 2.7 parts of alfalfa, 0.8 parts of Galla chinensis, 0.4 parts of Lycium barbarum and 0.5 parts of Hamamelis virginiana; wherein the mass ratio of the Ulmus pumila bark to the alfalfa is 1:3;

[0103] Step 2: Mix the herbal plants and crush them until the particle size of the herbal plants is no more than 0.1 mm to obtain a mixed material; mix the mixed material with water at a ratio of 1:45, heat to 55° C. and stir for 8 hours to obtain material A;

[0104] Step 3: sterilize material A with ultraviolet light, cool it to room temperature, inoculate fermentation bacteria to obtain material B, and perform anaerobic fermentation at 35°C and 55rpm for 22 hours to obtain a fermented material;

[0105] Among them, the fermentation bacteria is Lactobacillus reuteri CICC 6121; in material B, the fermentation bacteria content is 7.2×10 7 cfu / mL;

[0106] Step 4: sterilize the fermentation material, add ethanol to adjust the ethanol mass concentration to 43%, heat to 50°C, stir at 80rpm for 5 hours, and obtain material C;

[0107] Step 5: Cool material C to 30° C., filter using a filter membrane with a pore size of 0.22 μm to obtain a filtrate, and concentrate the filtrate under reduced pressure at a temperature of 60° C. and a pressure of 5.8 KPa to a mass of 12% of the mass of the filtrate to obtain a concentrate;

[0108] The concentrate is freeze-dried at -35°C to a water content of about 5.7% by mass; and then sterilized by ultraviolet light to obtain the composition 6.

[0109] 7. Composition 7

[0110] The preparation steps of the composition are as follows:

[0111] Step 1: Weigh the following dried herbal plants according to their mass: 1.2 parts of Polygonum multiflorum, 0.9 parts of Ulmus pumila bark, 0.8 parts of ginger, 2.7 parts of alfalfa, 0.8 parts of Galla chinensis, 0.4 parts of Lycium barbarum and 0.5 parts of Hamamelis virginiana; wherein the mass ratio of the Ulmus pumila bark to the alfalfa is 1:3;

[0112] Step 2: Mix the herbal plants and crush them until the particle size of the herbal plants is no more than 0.1 mm to obtain a mixed material; mix the mixed material with water at a ratio of 1:45, heat to 55° C. and stir for 8 hours to obtain material A;

[0113] Step 3: sterilize material A with ultraviolet light, cool it to room temperature, inoculate fermentation bacteria to obtain material B, and perform anaerobic fermentation at 35°C and 55rpm for 22 hours to obtain a fermented material;

[0114] Among them, the fermentation bacteria are Lactobacillus reuteri CICC 6121 and Lactobacillus acidophilus CICC 6081; in material B, the fermentation bacteria content is 7.2×10 7cfu / mL, the ratio of viable counts of Lactobacillus reuteri CICC 6121 and Lactobacillus acidophilus CICC 6081 was 0.1:1;

[0115] Step 4: sterilize the fermentation material, add ethanol to adjust the ethanol mass concentration to 43%, heat to 50°C, stir at 80rpm for 5 hours, and obtain material C;

[0116] Step 5: Cool material C to 30° C., filter using a filter membrane with a pore size of 0.22 μm to obtain a filtrate, and concentrate the filtrate under reduced pressure at a temperature of 60° C. and a pressure of 5.8 KPa to a mass of 12% of the mass of the filtrate to obtain a concentrate;

[0117] The concentrate is freeze-dried at -35°C to a water content of about 5.7% by mass; it is then sterilized by ultraviolet light to obtain the composition 7.

[0118] 8. Composition 8

[0119] The preparation steps of the composition are as follows:

[0120] Step 1: Weigh the following dried herbal plants according to their mass: 1.2 parts of Polygonum multiflorum, 0.9 parts of Ulmus pumila bark, 0.8 parts of ginger, 2.7 parts of alfalfa, 0.8 parts of Galla chinensis, 0.4 parts of Lycium barbarum and 0.5 parts of Hamamelis virginiana; wherein the mass ratio of the Ulmus pumila bark to the alfalfa is 1:3;

[0121] Step 2: Mix the herbal plants and crush them until the particle size of the herbal plants is no more than 0.1 mm to obtain a mixed material; mix the mixed material with water at a ratio of 1:45, heat to 55° C. and stir for 8 hours to obtain material A;

[0122] Step 3: sterilize material A with ultraviolet light, cool it to room temperature, inoculate fermentation bacteria to obtain material B, and perform anaerobic fermentation at 35°C and 55rpm for 22 hours to obtain a fermented material;

[0123] Among them, the fermentation bacteria are Lactobacillus reuteri CICC 6121 and Lactobacillus acidophilus CICC 6081; in material B, the fermentation bacteria content is 7.2×10 7 cfu / mL, the ratio of viable counts of Lactobacillus reuteri CICC 6121 and Lactobacillus acidophilus CICC 6081 was 3:1;

[0124] Step 4: sterilize the fermentation material, add ethanol to adjust the ethanol mass concentration to 43%, heat to 50°C, stir at 80rpm for 5 hours, and obtain material C;

[0125] Step 5: Cool material C to 30° C., filter using a filter membrane with a pore size of 0.22 μm to obtain a filtrate, and concentrate the filtrate under reduced pressure at a temperature of 60° C. and a pressure of 5.8 KPa to a mass of 12% of the mass of the filtrate to obtain a concentrate;

[0126] The concentrate is freeze-dried at -35°C to a water content of about 5.7% by weight; and then sterilized by ultraviolet light to obtain the composition 8.

[0127] 9. Composition 9

[0128] The preparation steps of the composition are as follows:

[0129] Step 1: Weigh the following dried herbal plants according to mass: 1.2 parts of Polygonum multiflorum, 1.8 parts of Ulmus pumila bark, 0.8 parts of ginger, 1.8 parts of alfalfa, 0.8 parts of Galla chinensis, 0.4 parts of Lycium barbarum and 0.5 parts of Hamamelis virginiana; wherein the mass ratio of the Ulmus pumila bark to the alfalfa is 1:1;

[0130] Step 2: Mix the herbal plants and crush them until the particle size of the herbal plants is no more than 0.1 mm to obtain a mixed material; mix the mixed material with water at a ratio of 1:45, heat to 55° C. and stir for 8 hours to obtain material A;

[0131] Step 3: sterilize material A with ultraviolet light, cool it to room temperature, inoculate fermentation bacteria to obtain material B, and perform anaerobic fermentation at 35°C and 55rpm for 22 hours to obtain a fermented material;

[0132] Among them, the fermentation bacteria are Lactobacillus reuteri CICC 6121 and Lactobacillus acidophilus CICC 6081; in material B, the fermentation bacteria content is 7.2×10 7 cfu / mL, the ratio of viable counts of Lactobacillus reuteri CICC 6121 and Lactobacillus acidophilus CICC 6081 was 0.8:1;

[0133] Step 4: sterilize the fermentation material, add ethanol to adjust the ethanol mass concentration to 43%, heat to 50°C, stir at 80rpm for 5 hours, and obtain material C;

[0134] Step 5: Cool material C to 30° C., filter using a filter membrane with a pore size of 0.22 μm to obtain a filtrate, and concentrate the filtrate under reduced pressure at a temperature of 60° C. and a pressure of 5.8 KPa to a mass of 12% of the mass of the filtrate to obtain a concentrate;

[0135] The concentrate is freeze-dried at -35°C to a water content of about 5.7% by weight; and then sterilized by ultraviolet light to obtain the composition 9.

[0136] 10. Composition 10

[0137] The preparation steps of the composition are as follows:

[0138] Step 1: Weigh the following dried herbal plants according to mass: 1.2 parts of Polygonum multiflorum, 0.4 parts of Ulmus pumila bark, 0.8 parts of ginger, 3.2 parts of alfalfa, 0.8 parts of Galla chinensis, 0.4 parts of Lycium barbarum and 0.5 parts of Hamamelis virginiana; wherein the mass ratio of the Ulmus pumila bark to the alfalfa is 1:8;

[0139] Step 2: Mix the herbal plants and crush them until the particle size of the herbal plants is no more than 0.1 mm to obtain a mixed material; mix the mixed material with water at a ratio of 1:45, heat to 55° C. and stir for 8 hours to obtain material A;

[0140] Step 3: sterilize material A with ultraviolet light, cool it to room temperature, inoculate fermentation bacteria to obtain material B, and perform anaerobic fermentation at 35°C and 55rpm for 22 hours to obtain a fermented material;

[0141] Among them, the fermentation bacteria are Lactobacillus reuteri CICC 6121 and Lactobacillus acidophilus CICC 6081; in material B, the fermentation bacteria content is 7.2×10 7 cfu / mL, the ratio of viable counts of Lactobacillus reuteri CICC 6121 and Lactobacillus acidophilus CICC 6081 was 0.8:1;

[0142] Step 4: sterilize the fermentation material, add ethanol to adjust the ethanol mass concentration to 43%, heat to 50°C, stir at 80rpm for 5 hours, and obtain material C;

[0143] Step 5: Cool material C to 30° C., filter using a filter membrane with a pore size of 0.22 μm to obtain a filtrate, and concentrate the filtrate under reduced pressure at a temperature of 60° C. and a pressure of 5.8 KPa to a mass of 12% of the mass of the filtrate to obtain a concentrate;

[0144] The concentrate is freeze-dried at -35°C to a water content of about 5.7% by weight; and then sterilized by ultraviolet light to obtain the composition 10.

[0145] 11. Composition 11

[0146] The preparation steps of the composition are as follows:

[0147] Step 1: Weigh the following dried herbal plants according to their mass: 1.2 parts of Polygonum multiflorum, 0.9 parts of Ulmus pumila bark, 0.8 parts of ginger, 2.7 parts of alfalfa, 0.8 parts of Galla chinensis, 0.4 parts of Lycium barbarum and 0.5 parts of Hamamelis virginiana; wherein the mass ratio of the Ulmus pumila bark to the alfalfa is 1:3;

[0148] Step 2: Mix the herbal plants and crush them until the particle size of the herbal plants is no more than 0.1 mm to obtain a mixed material; mix the mixed material with water at a ratio of 1:45, heat to 55° C. and stir for 8 hours to obtain material A;

[0149] Step 3: sterilize material A with ultraviolet light, cool it to room temperature, inoculate fermentation bacteria to obtain material B, and perform anaerobic fermentation at 35°C and 55rpm for 22 hours to obtain a fermented material;

[0150] Among them, the fermentation bacteria are Lactobacillus reuteri CICC 6121 and Lactobacillus acidophilus CICC 6081; in material B, the fermentation bacteria content is 7.2×10 7 cfu / mL, the ratio of viable counts of Lactobacillus reuteri CICC 6121 and Lactobacillus acidophilus CICC 6081 was 0.8:1;

[0151] Step 4: sterilize the fermentation material, add ethanol, adjust the ethanol mass concentration to 10%, heat to 50°C, stir at 80rpm for 5 hours, and obtain material C;

[0152] Step 5: Cool material C to 30° C., filter using a filter membrane with a pore size of 0.22 μm to obtain a filtrate, and concentrate the filtrate under reduced pressure at a temperature of 60° C. and a pressure of 5.8 KPa to a mass of 12% of the mass of the filtrate to obtain a concentrate;

[0153] The concentrate is freeze-dried at -35°C to a water content of about 5.7% by mass; and then sterilized by ultraviolet light to obtain the composition 11.

[0154] 12. Composition 12

[0155] The preparation steps of the composition are as follows:

[0156] Step 1: Weigh the following dried herbal plants according to their mass: 1.2 parts of Polygonum multiflorum, 0.9 parts of Ulmus pumila bark, 0.8 parts of ginger, 2.7 parts of alfalfa, 0.8 parts of Galla chinensis, 0.4 parts of Lycium barbarum and 0.5 parts of Hamamelis virginiana; wherein the mass ratio of the Ulmus pumila bark to the alfalfa is 1:3;

[0157] Step 2: Mix the herbal plants and crush them until the particle size of the herbal plants is no more than 0.1 mm to obtain a mixed material; mix the mixed material with water at a ratio of 1:45, heat to 55° C. and stir for 8 hours to obtain material A;

[0158] Step 3: sterilize material A with ultraviolet light, cool it to room temperature, inoculate fermentation bacteria to obtain material B, and perform anaerobic fermentation at 35°C and 55rpm for 22 hours to obtain a fermented material;

[0159] Among them, the fermentation bacteria are Lactobacillus reuteri CICC 6121 and Lactobacillus acidophilus CICC 6081; in material B, the fermentation bacteria content is 7.2×10 7 cfu / mL, the ratio of viable counts of Lactobacillus reuteri CICC 6121 and Lactobacillus acidophilus CICC 6081 was 0.8:1;

[0160] Step 4: sterilize the fermentation material, add ethanol to adjust the ethanol mass concentration to 80%, heat to 50°C, stir at 80rpm for 5 hours, and obtain material C;

[0161] Step 5: Cool material C to 30° C., filter using a filter membrane with a pore size of 0.22 μm to obtain a filtrate, and concentrate the filtrate under reduced pressure at a temperature of 60° C. and a pressure of 5.8 KPa to a mass of 12% of the mass of the filtrate to obtain a concentrate;

[0162] The concentrate is freeze-dried at -35°C to a water content of about 5.7% by mass; and then sterilized by ultraviolet light to obtain the composition 12.

[0163] 13. Composition 13

[0164] The preparation steps of the composition are as follows:

[0165] Step 1: Weigh the following dried herbal plants according to their mass: 1.2 parts of Polygonum multiflorum, 0.9 parts of Ulmus pumila bark, 0.8 parts of ginger, 2.7 parts of alfalfa, 0.8 parts of Galla chinensis, 0.4 parts of Lycium barbarum and 0.5 parts of Hamamelis virginiana; wherein the mass ratio of the Ulmus pumila bark to the alfalfa is 1:3;

[0166] Step 2: Mix the herbal plants and crush them until the particle size of the herbal plants is no more than 0.1 mm to obtain a mixed material; mix the mixed material with water at a ratio of 1:45, heat to 55° C. and stir for 8 hours to obtain material A;

[0167] Step 3: sterilize material A, add ethanol to adjust the ethanol mass concentration to 43%, heat to 50°C, stir at 80rpm for 5 hours, and obtain material C;

[0168] Step 5: Cool material C to 30° C., filter using a filter membrane with a pore size of 0.22 μm to obtain a filtrate, and concentrate the filtrate under reduced pressure at a temperature of 60° C. and a pressure of 5.8 KPa to a mass of 12% of the mass of the filtrate to obtain a concentrate;

[0169] The concentrate is freeze-dried at -35°C to a water content of about 5.7% by mass; and then sterilized by ultraviolet light to obtain the composition 13.

[0170] 14. Composition 14

[0171] The preparation steps of the composition are as follows:

[0172] Step 1: Weigh the following dried herbal plants according to their mass: 1.2 parts of Polygonum multiflorum, 0.8 parts of ginger, 0.8 parts of Galla chinensis, 0.4 parts of Lycium barbarum and 0.5 parts of Hamamelis virginiana;

[0173] Step 2: Mix the herbal plants and crush them until the particle size of the herbal plants is no more than 0.1 mm to obtain a mixed material; mix the mixed material with water at a ratio of 1:45, heat to 55° C. and stir for 8 hours to obtain material A;

[0174] Step 3: sterilize material A with ultraviolet light, cool it to room temperature, inoculate fermentation bacteria to obtain material B, and perform anaerobic fermentation at 35°C and 55rpm for 22 hours to obtain a fermented material;

[0175] Among them, the fermentation bacteria are Lactobacillus reuteri CICC 6121 and Lactobacillus acidophilus CICC 6081; in material B, the fermentation bacteria content is 7.2×10 7 cfu / mL, the ratio of viable counts of Lactobacillus reuteri CICC 6121 and Lactobacillus acidophilus CICC 6081 was 0.8:1;

[0176] Step 4: sterilize the fermentation material, add ethanol to adjust the ethanol mass concentration to 43%, heat to 50°C, stir at 80rpm for 5 hours, and obtain material C;

[0177] Step 5: Cool material C to 30° C., filter using a filter membrane with a pore size of 0.22 μm to obtain a filtrate, and concentrate the filtrate under reduced pressure at a temperature of 60° C. and a pressure of 5.8 KPa to a mass of 12% of the mass of the filtrate to obtain a concentrate;

[0178] The concentrate is freeze-dried at -35°C to a water content of about 5.7% by mass; it is then sterilized by ultraviolet light to obtain the composition 14.

[0179] 15. Composition 15

[0180] The preparation steps of the composition are as follows:

[0181] Step 1: Weigh the following dried herbal plants according to their mass: 1.2 parts of Polygonum multiflorum, 0.8 parts of ginger, 3.6 parts of alfalfa, 0.8 parts of Galla chinensis, 0.4 parts of Lycium barbarum and 0.5 parts of Hamamelis Virginiana;

[0182] Step 2: Mix the herbal plants and crush them until the particle size of the herbal plants is no more than 0.1 mm to obtain a mixed material; mix the mixed material with water at a ratio of 1:45, heat to 55° C. and stir for 8 hours to obtain material A;

[0183] Step 3: sterilize material A with ultraviolet light, cool it to room temperature, inoculate fermentation bacteria to obtain material B, and perform anaerobic fermentation at 35°C and 55rpm for 22 hours to obtain a fermented material;

[0184] Among them, the fermentation bacteria are Lactobacillus reuteri CICC 6121 and Lactobacillus acidophilus CICC 6081; in material B, the fermentation bacteria content is 7.2×10 7 cfu / mL, the ratio of viable counts of Lactobacillus reuteri CICC 6121 and Lactobacillus acidophilus CICC 6081 was 0.8:1;

[0185] Step 4: sterilize the fermentation material, add ethanol to adjust the ethanol mass concentration to 43%, heat to 50°C, stir at 80rpm for 5 hours, and obtain material C;

[0186] Step 5: Cool material C to 30° C., filter using a filter membrane with a pore size of 0.22 μm to obtain a filtrate, and concentrate the filtrate under reduced pressure at a temperature of 60° C. and a pressure of 5.8 KPa to a mass of 12% of the mass of the filtrate to obtain a concentrate;

[0187] The concentrate is freeze-dried at -35°C to a water content of about 5.7% by mass; and then sterilized by ultraviolet light to obtain the composition 15.

[0188] 16. Composition 16

[0189] The preparation steps of the composition are as follows:

[0190] Step 1: Weigh the following dried herbal plants according to their mass: 1.2 parts of Polygonum multiflorum, 3.6 parts of Ulmus pumila bark, 0.8 parts of ginger, 0.8 parts of Galla chinensis, 0.4 parts of Lycium barbarum and 0.5 parts of Hamamelis virginiana;

[0191] Step 2: Mix the herbal plants and crush them until the particle size of the herbal plants is no more than 0.1 mm to obtain a mixed material; mix the mixed material with water at a ratio of 1:45, heat to 55° C. and stir for 8 hours to obtain material A;

[0192] Step 3: sterilize material A with ultraviolet light, cool it to room temperature, inoculate fermentation bacteria to obtain material B, and perform anaerobic fermentation at 35°C and 55rpm for 22 hours to obtain a fermented material;

[0193] Among them, the fermentation bacteria are Lactobacillus reuteri CICC 6121 and Lactobacillus acidophilus CICC 6081; in material B, the fermentation bacteria content is 7.2×10 7 cfu / mL, the ratio of viable counts of Lactobacillus reuteri CICC 6121 and Lactobacillus acidophilus CICC 6081 was 0.8:1;

[0194] Step 4: sterilize the fermentation material, add ethanol to adjust the ethanol mass concentration to 43%, heat to 50°C, stir at 80rpm for 5 hours, and obtain material C;

[0195] Step 5: Cool material C to 30° C., filter using a filter membrane with a pore size of 0.22 μm to obtain a filtrate, and concentrate the filtrate under reduced pressure at a temperature of 60° C. and a pressure of 5.8 KPa to a mass of 12% of the mass of the filtrate to obtain a concentrate;

[0196] The concentrate is freeze-dried at -35°C to a water content of about 5.7% by mass; it is then sterilized by ultraviolet light to obtain the composition 16.

[0197] 2. Effect Test

[0198] 1. Anti-hair loss and hair growth effect test

[0199] The time it takes for the skin of depilated mice to turn from pink to black and the time it takes for hair to grow out are used to measure the product's anti-hair loss and hair growth effects.

[0200] Several SPF-grade 7-9 week-old C57BL / 6 male mice were selected. One day before the experiment, the mice were anesthetized with 4wt% chloral hydrate, and the hair of the same 2.5×2.5cm area on the back was depilated. On the second day of depilation, mice with smooth skin and no wounds in the depilated area were selected and randomly divided into blank group, model group, drug group and combination group 1-16 according to body weight, a total of 19 groups, with 10 mice in each group.

[0201] Except for the blank group, 120 μL of testosterone solution with a concentration of 0.055wt% was applied to the depilated area on the back of the mice in the other groups once a day for 15 consecutive days. After 1 hour of testosterone solution treatment, 120 μL of the smear solution was applied to the depilated area in the same way, once a day for 15 consecutive days. The smear solution in the drug group was a 2wt% finasteride solution, and the smear solution in the composition 1-16 group was an aqueous solution with a concentration of 8wt% of the composition; the blank group and the model group were given the same volume of deionized water.

[0202] After the administration, the skin and hair growth of the hair removal area of ​​each group of mice were observed every day, and the time when the skin color of the hair removal area of ​​each mouse changed from pink to black and the time when hair began to grow were recorded to evaluate the effect of the drug on the hair growth cycle of mice. The test results of the hair growth effect of each group of mice are shown in Table 1.

[0203] Table 1: Test results of hair growth effect on mice in each group

[0204]

[0205] The data in Table 1 were converted to calculate the advance time for skin darkening in the test area of ​​each group of mice compared with the model group and the advance time for hair growth in the test area of ​​each group of mice compared with the model group. The results are shown in Table 2.

[0206] Table 2: Advance time of each group compared with the model group

[0207]

[0208] The test results in Table 1-2 show that the mouse experiment test shows that the composition of the present invention causes the skin of the depilated mice to change from pink to black on the 7.213±0.283th day, and causes the skin in the depilated area to start growing new hair on the 10.583±0.614th day; compared with the model group, the composition of the present invention causes the mouse skin to change from pink to black 1.741 days earlier, and causes the mouse skin to grow hair 1.993 days earlier. It can be seen that the composition of the present invention has a good effect of preventing hair loss and promoting hair growth.

[0209] By comparing the experimental data of compositions 1 and 13, it can be seen that the fermentation process used in the preparation method of the present invention can significantly improve the effect of the composition in promoting hair growth. And by comparing the test data of compositions 1, 3, and 4, it can be seen that the fermentation bacteria selected in the present invention make the composition prepared by the present invention more effective than other fermentation bacteria. The present invention uses Lactobacillus mucilaginosus CICC 6121 and Lactobacillus acidophilus CICC 6081 for co-fermentation, which increases the concentration and activity of the hair growth active ingredients of herbal plants, and the two synergistically promote the combined hair growth effect; and the ratio of the number of fermentation bacteria of the two also affects the effect of the composition. The above content can be obtained in combination with the comparison of compositions 1, 5-8.

[0210] Furthermore, through the test results of compositions 1, 2, 9-16, it can be seen that in the preparation system of the present invention, the combination and dosage ratio of the preparation raw materials elm bark and alfalfa, as well as the concentration of ethanol used in the extraction process, all have an impact on the hair growth effect of the composition; in the present invention, the dosage ratio of elm bark and alfalfa is 1:2-4 and the ethanol concentration during extraction is 32-55%, and the hair growth effect of the obtained composition is better.

[0211] 2. Hair darkening effect test

[0212] The hair darkening effect of the composition of the present invention is verified by testing the tyrosinase activation rate; wherein, the higher the tyrosinase activation rate, the stronger the ability to promote melanin production, indicating that the hair darkening effect of the composition of the present invention is better.

[0213] A 1 g / L L-DOPA solution was prepared using a 0.2 mol / L phosphate buffer solution with a pH of 6.8. A tyrosinase solution with a concentration of 100 U / mL was prepared using a 0.2 mol / L phosphate buffer solution with a pH of 6.8. A test solution of the above composition 1-16 with a concentration of 0.25 mg / mL was prepared using a 0.2 mol / L phosphate buffer solution with a pH of 6.8. 40 μL of L-DOPA solution, 40 μL of the test solution, and 40 μL of the tyrosinase solution were added to a 96-well plate and placed in a 30°C water bath for 40 min. After the reaction was completed, the absorbance was tested at 490 nm, which was the absorbance C. Referring to the above method, phosphate buffer was used instead of the tyrosinase solution, and the absorbance was tested to be D; phosphate buffer was used instead of the test solution, and the absorbance was tested to be A; phosphate buffer was used instead of the test solution and the tyrosinase solution, and the absorbance was tested to be B. The tyrosinase activation rate calculation formula is: tyrosinase activation rate / % = [(CD)-(AB)] / (AB)×100%. The specific test results are shown in Table 3.

[0214] Table 3: Tyrosinase activation rate test results

[0215]

[0216] According to the contents of Table 3, the composition prepared by the present invention can effectively promote the activation of tyrosinase and has a good hair darkening effect. The tyrosinase activation rate test of the present invention shows that the composition of the present invention makes the tyrosinase activation rate reach 187.43%.

[0217] 3. Skin irritation test

[0218] Several volunteers aged 30-55 were selected and randomly divided into 16 groups, with 4 male and 4 female volunteers in each group. 25 μL of the aqueous solution (concentration 1wt%) of the above composition 1-16 was respectively dripped on the filter paper and placed in the spot tester, and then the spot tester was taped to the same part of the right arm of each group of volunteers, and pressed evenly with the palm, and then continued for 24 hours; the spot tester was removed for half an hour, and the skin reaction was observed and evaluated according to the standards in Table 4. The results of the skin irritation test after removing the spot tester for half an hour are shown in Table 5.

[0219] Table 4: Evaluation criteria

[0220]

[0221] Table 5: Skin irritation test results

[0222]

[0223] According to the contents of Table 5, the composition prepared by the present invention has low irritation, and the skin irritation test shows that the irritation level is level 0.

[0224] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A method for preparing a herbal composition for preventing hair loss, promoting hair growth and darkening hair, characterized in that: The method comprises the following preparation steps: Step 1: Weigh the following dried herbal plants according to weight: 0.5-2 parts of Polygonum multiflorum, 0.4-1.8 parts of Elm bark, 0.5-1.5 parts of ginger, 1-3 parts of alfalfa, 0.5-2.2 parts of Galla chinensis, 0.1-0.8 parts of Lycium barbarum, and 0.1-0.6 parts of Hamamelis virginiana; Step 2: Mix the herbal plants and crush them to obtain a mixed material; mix the mixed material with water at a mass ratio of 1:15-100, heat to 40-65° C. and stir for 5-15 hours to obtain material A; Step 3: sterilize material A, cool it, inoculate it with fermentation bacteria to obtain material B, and perform anaerobically fermentation at 28-40°C for 10-30 hours to obtain a fermented material; Step 4: sterilize the fermentation material, add ethanol, adjust the ethanol mass concentration to 32-55%, heat and stir to obtain material C; Step 5: filtering material C to obtain a filtrate, concentrating the filtrate, drying, and sterilizing to obtain the composition; Among them, the fermentation bacteria are Lactobacillus mucilaginosus CICC 6121 and Lactobacillus acidophilus CICC 6081; In material B, the fermentation bacteria content is 3×10 7 -9×10 7 cfu / mL; In material B, the ratio of the number of live bacteria of Lactobacillus reuteri CICC 6121 and Lactobacillus acidophilus CICC 6081 is 0.5-1:1; The mass ratio of elm bark to alfalfa is 1:2-4.

2. The preparation method according to claim 1, characterized in that In step 2, the mass ratio of the mixed material to water is 1:20-80.

3. The preparation method according to claim 1, characterized in that: In step 2, the mixture is heated to 55-62° C. and stirred for 8-12 hours.

4. The preparation method according to claim 1, characterized in that: In step 3, anaerobic fermentation is performed at 32-40°C for 12-18 hours.

5. The preparation method according to claim 1, characterized in that: In step 4, the ethanol mass concentration is adjusted to 35-50%; In step 4, the heating and stirring is heating to 42-60° C. and stirring for 4-10 hours.

6. The preparation method according to claim 1, characterized in that: In step 5, the drying is freeze-drying.

7. A herbal composition for preventing hair loss, promoting hair growth and darkening hair prepared by the preparation method according to any one of claims 1 to 6.

8. Use of the composition according to claim 7 in preparing cosmetics, characterized in that: The cosmetic is at least one of essence, cream, ointment and powder.

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