Tumor treatment device

Abstract

The invention provides a tumor treatment device. The tumor treatment device comprises an alternating current signal generating device, a transmitting device and a receiving device, the alternating current signal generating device is connected with the transmitting device, and a boosting structure is arranged in the transmitting device and used for converting the intermediate-frequency low-voltage alternating current signal into a first voltage signal; the output end of the boosting structure is connected with a first electrode; the receiving device is arranged opposite to the transmitting device and is connected with the alternating current signal generating device, and a voltage transformation structure is arranged in the receiving device; the output end of the voltage transformation structure is connected with a second electrode, and the voltage transformation structure is used for receiving an induced voltage signal loaded by the voltage transformation structure and is coupled with the first electrode so as to emit electromagnetic wave signals with various frequencies. Therefore, the target object receives the electromagnetic wave radiation at the optimal position, and the mitosis of tumor tissues and cells is inhibited.

Description

Technical Field

[0001] The present invention relates to the technical field of medical equipment, and in particular to a tumor treatment device. Background Art

[0002] Using electric fields to treat tumors is one of the current research and development frontiers. Tumor electric field therapy is a therapy implemented through portable, non-invasive medical devices. Its basic principle is based on the fact that electric fields hinder the mitosis of tumor cells. Different frequencies, directions and intensities of electric fields all show different tumor inhibition effects.

[0003] TTFields is a portable, non-invasive local physical therapy that generates low-intensity, medium-frequency electric fields of specific frequencies by placing specially-made insulated electrode patches on the patient's skin surface. These electric fields can penetrate the skin and body tissues and act directly on the tumor site, thereby interfering with the mitosis of tumor cells and ultimately inhibiting tumor growth and spread.

[0004] TTFields was approved by the U.S. Food and Drug Administration (FDA) as early as 2015 for the treatment of newly diagnosed glioblastoma (GBM), and has since been approved for the treatment of recurrent glioblastoma (rGBM) and malignant pleural mesothelioma. In addition, this therapy can also be used to treat extracranial tumors such as non-small cell lung cancer, pancreatic cancer, gastric cancer, liver cancer, and ovarian cancer.

[0005] The main products of the existing tumor electric field therapy devices are the tumor electric field therapy products (Optune) developed by Novocure, an American biotechnology company, which mainly include signal generators and electrodes. During the electric field therapy process, the signal generator generates electric field energy, which is distributed to the electrodes. The electrodes couple the electric field energy into the human body, thereby applying the alternating electric field to the tissue area where the tumor is located.

[0006] However, TTFields has the following three major disadvantages when used: First, the electrodes need to be attached to the human skin during treatment. If the patient's head is treated for glioma, the patient's hair needs to be shaved and electrodes are attached to the shaved scalp. The low-intensity, medium-frequency AC electric field sent by the electrodes selectively destroys cell division. According to the latest clinical data feedback, there are adverse reactions during TTF treatment, namely moderate skin irritation, contact dermatitis and other symptoms.

[0007] Secondly, because it is a contact treatment, due to space and technical limitations, TTFields cannot apply a larger electric field and cannot produce greater tumor cell killing ability. It can only inhibit the tumor division rate and often needs to be used in combination with other treatment options (drugs, chemotherapy).

[0008] Finally, TTFields needs to be worn for more than 18 hours a day, which seriously affects the patient's quality of life and the patient experience is not good. Summary of the invention

[0009] In view of this, the present invention proposes a tumor treatment device, aiming to solve the problem that the tumor treatment device in the prior art has a poor user experience and generates a weak electric field. The present invention proposes a tumor treatment device, comprising: an AC signal generating device, a transmitting device and a receiving device; wherein: The AC signal generating device is connected to the transmitting device, and is used to generate a medium-frequency low-voltage AC signal and transmit the medium-frequency low-voltage AC signal to the transmitting device; The transmitting device is provided with a boost structure for converting the medium-frequency low-voltage AC power signal into a first voltage signal; the voltage value of the first voltage signal is greater than the voltage value of the medium-frequency low-voltage AC power signal, and the output end of the boost structure is connected to a first electrode for receiving the first voltage signal loaded by the boost structure; The receiving device is arranged opposite to the transmitting device and connected to the AC signal generating device. A transformer structure is arranged in the receiving device for matching with the boost structure to generate an induced voltage signal. The voltage value of the induced voltage signal is smaller than the voltage value of the first voltage signal, thereby generating an electric field with high field strength between the transmitting device and the receiving device. The output end of the transformer structure is connected to a second electrode, the second electrode is used to receive the induced voltage signal loaded by the transformer structure and couple with the first electrode to emit electromagnetic wave signals of multiple frequencies; The first electrode and the second electrode both have a frame structure, a notch is provided at the end of the frame structure, and arc-shaped contacts are provided at both ends of the notch to prevent arc discharge; A seat mechanism is arranged in the area between the receiving device and the transmitting device, so as to enable the target object to receive high-field-strength and multi-frequency electromagnetic wave radiation at an optimal position.

[0010] Furthermore, in the above tumor treatment device, the frame structure is a ring frame, and there are multiple ring frames, and the multiple ring frames are concentrically arranged to form a concentric circle structure, and the contact connection lines at both ends of each notch are arranged at an angle.

[0011] Furthermore, in the above-mentioned tumor treatment device, a first fastener is provided on the frame structure of the first electrode for connecting with the transmitting device; and / or a second fastener is provided on the frame structure of the second electrode for connecting with the receiving device.

[0012] Furthermore, in the above tumor treatment device, the first fastener and the second fastener each include: at least two intersecting connecting plates; wherein: Each of the connecting plates is provided with a plurality of slots or connecting holes corresponding to each other, so as to be fixed to the corresponding frame structure respectively.

[0013] Furthermore, in the above tumor treatment device, the first electrode is electrically connected to the output end of the boost structure through a first high-voltage wire; and / or the second electrode is electrically connected to the output end of the transformer structure through a second high-voltage wire.

[0014] Furthermore, in the above tumor treatment device, the boost structure and the transformer structure are both Tesla coils, and the frequencies of the two Tesla coils are the same.

[0015] Furthermore, in the above tumor treatment device, the transmitting device and the receiving device are both provided with an insulating shell, and a roller is provided at the bottom of the insulating shell.

[0016] Furthermore, in the above tumor treatment device, indicator plates are respectively provided on the insulating shell in the areas corresponding to the first electrode and the second electrode, so as to align the user's part to be treated with the areas where the first electrode and the second electrode are located.

[0017] Furthermore, in the above tumor treatment device, the indicator plate is circular or polygonal.

[0018] Furthermore, in the above-mentioned tumor treatment device, a first support frame is arranged in the insulating shell of the transmitting device, and the boosting structure is arranged on the first support frame; and / or a second support frame is arranged in the insulating shell of the receiving device, and the transformer structure is arranged on the second support frame.

[0019] The tumor treatment device provided by the present invention boosts the medium-frequency low-voltage alternating current signal to a first voltage signal with a higher voltage value through a boost structure in a transmitting device, and loads it to a first electrode. At the same time, the transformer structure and the boost structure in the receiving device cooperate to generate an induced voltage with a voltage value lower than the first voltage signal, and load it to a second electrode. The second electrode is coupled with the first electrode and emits electromagnetic wave signals of multiple frequencies, so that the target object receives the electromagnetic wave radiation at the optimal position, thereby inhibiting the mitosis of its tumor tissue and cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Various other advantages and benefits will become apparent to those of ordinary skill in the art by reading the detailed description of the preferred embodiments below. The accompanying drawings are only for the purpose of illustrating the preferred embodiments and are not to be considered as limiting the present invention. Moreover, the same reference symbols are used throughout the accompanying drawings to represent the same components. In the accompanying drawings: Figure 1 A schematic diagram of the structure of a tumor treatment device provided by an embodiment of the present invention; Figure 2 An axial view of the interior of a transmitting device or a receiving device in an embodiment of the present invention; Figure 3 is a schematic structural diagram of a first electrode or a second electrode in an embodiment of the present invention; Figure 4 is an equivalent electronic circuit diagram of the resonance process of the first electrode and the second electrode in an embodiment of the present invention; Figure 5 is a waveform diagram of high-field-strength multi-frequency electromagnetic waves in an embodiment of the present invention; Figure 6 A graph comparing the growth curve of U251 glioma cells treated with the non-invasive tumor treatment device provided by an embodiment of the present invention and the growth curve of U251 glioma cells in a normal incubator; Figure 7 A graph comparing the apoptosis curve of U251 glioma cells treated by the non-invasive tumor treatment device provided in an embodiment of the present invention and the apoptosis curve of U251 glioma cells in a normal incubator; Figure 8 A graph comparing the apoptosis curve of MCF7 breast cancer cells treated with the non-invasive tumor treatment device provided in an embodiment of the present invention and the apoptosis curve of MCF7 breast cancer cells in a normal incubator; Fig. 9 This is a comparison chart of the apoptosis curve of HELA cells treated with the non-invasive tumor treatment device provided in the embodiment of the present invention and the apoptosis curve of HELA cells in a normal incubator. DETAILED DESCRIPTION

[0021] Exemplary embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings. Although exemplary embodiments of the present disclosure are shown in the accompanying drawings, it should be understood that the present disclosure can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided in order to enable a more thorough understanding of the present disclosure and to be able to fully convey the scope of the present disclosure to those skilled in the art. It should be noted that, in the absence of conflict, the embodiments of the present invention and the features described in the embodiments can be combined with each other. The present invention will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0022] See also Figures 1 to 3The tumor treatment device of the embodiment of the present invention comprises: an AC signal generating device 1, a transmitting device 2 and a receiving device 3; wherein the AC signal generating device 1 is connected to the transmitting device 2 to generate a medium-frequency low-voltage AC signal and transmit the medium-frequency low-voltage AC signal to the transmitting device 2, and a boost structure 21 is provided in the transmitting device 2 to convert the medium-frequency low-voltage AC signal into a first voltage signal; the voltage value of the first voltage signal is greater than the voltage value of the medium-frequency low-voltage AC signal, and the output end of the boost structure 21 is connected to a first electrode 41 for receiving the first voltage signal loaded by the boost structure 21; the receiving device 3 is arranged opposite to the transmitting device 2 and connected to the AC signal generating device 1, and a transformer structure 31 is provided in the receiving device 3 to The boost structure 21 matches to generate an induced voltage signal, the voltage value of the induced voltage signal is less than the voltage value of the first voltage signal; thereby generating a high-field-strength electric field between the transmitting device 2 and the receiving device 3; the output end of the transformer structure 31 is connected to a second electrode 42, the second electrode 42 is used to receive the induced voltage signal loaded by the transformer structure 31 and couple with the first electrode 41 to transmit electromagnetic wave signals of multiple frequencies; the first electrode 41 and the second electrode 42 both have a frame structure 40, the end of the frame structure 40 is provided with a notch a, and both ends of the notch a are provided with arc contacts b to prevent arc discharge; the area between the receiving device 3 and the transmitting device 2 is provided with a seat mechanism to enable the target object to receive high-field-strength and multi-frequency electromagnetic wave radiation in the best position.

[0023] Specifically, the transmitting device 2 and the receiving device 3 may be a cabinet structure, such as a square cabinet.

[0024] The first electrode 41 is electrically connected to the output end of the boost structure 21 via a first high-voltage wire (not shown in the figure); and / or the second electrode 42 is electrically connected to the output end of the transformer structure 31 via a second high-voltage wire (not shown in the figure).

[0025] The input end of the boost structure 21 can be connected to the AC signal generator 1 through the first control line 7, and the input end of the transformer structure 31 can be connected to the AC signal generator 1 through the second control line 8. The first control line 7 is inserted into the column of the first support frame 5 inside the insulating shell, and the second control line 8 is inserted into the column of the second support frame 6 inside the insulating shell. The AC signal generator 1 can be connected to an external 220V power supply.

[0026] The frame structure 40 of the first electrode 41 and the second electrode 42 may be a ring frame or a polygonal frame, such as a square frame, a trapezoidal frame, a rectangular frame, etc. Preferably, the frame structure is a ring frame. There may be one or more frame structures 40.

[0027] In this embodiment, there is capacitance to the ground between the frame structure of the first electrode 41 and the frame structure of the second electrode 42, and the Tesla coils that form the boost structure and the Tesla coils that form the transformer structure form a circuit resonance process with damping. Figure 4 , which can be described by a linear second-order differential equation: In the formula: R is the resistance, Uc represents the effective value of the voltage on the capacitor, L is the inductance value, C is the capacitance value, I is the current, U is the voltage value, and t is the time.

[0028] Final Formation Figure 5 From the damped oscillation attenuation waveform in the figure, it can be seen that the capacitance, voltage and current are all equal-amplitude oscillations, the voltage and current are 90° out of phase with each other, the capacitance voltage oscillates between U0 and -U0 according to the sinusoidal law, and the current oscillates around the zero value according to the sinusoidal law.

[0029] Electromagnetic wave signals based on this waveform are not ideal in the process of propagation in space. The amplitude-frequency characteristics of capacitors and inductors are mainly reflected in their responses to signals of different frequencies. In AC circuits, the response characteristics of capacitors and inductors to frequency are different: capacitors have the characteristics of "passing high frequencies and blocking low frequencies". In AC circuits, the impedance of capacitors decreases with the increase of frequency, so capacitors have less blocking effect on high-frequency signals and greater blocking effect on low-frequency signals. When the amplitude changes, the frequency characteristics of capacitors and inductors at different amplitudes will not change directly due to changes in the amplitude of the signal. Ideally, the impedance of capacitors and inductors is a function of frequency, not a function of signal amplitude. That is, for a given frequency, the impedance of capacitors and inductors remains unchanged, but in practical applications, when the signal amplitude changes, some non-ideal effects may be observed, which can indirectly affect the appearance of capacitors and inductors, resulting in frequency response. Therefore, under non-ideal conditions, changes in the amplitude of electromagnetic waves will cause changes in the capacitance and inductance characteristics, thereby causing changes in frequency, thereby forming multi-frequency electromagnetic wave signals. In this embodiment, the frequency of the electromagnetic wave signal is between 10khz-1000Khz.

[0030] The target object is placed in the high-field electric field environment formed between the transmitting device and the receiving device in the embodiment of the present invention, and receives the medium-frequency electromagnetic wave energy and signal. The medium-frequency electromagnetic wave causes polarization of the biological tissue medium through electromagnetic attraction, thereby changing the distance between atoms and atomic groups in the molecules, thereby changing the electromagnetic properties of the tissue such as the dielectric constant and magnetic permeability.

[0031] At the same time, proteins, lipid biomolecules and amino acid biomolecules absorb energy from radio frequency electromagnetic waves through frequency resonance, which causes changes in their own structure, conformation and configuration. After absorbing energy, their dipole moment, dielectric constant and magnetic permeability also change strongly with frequency. This change can affect cell mitochondria, provide energy to cells, and eliminate common inflammation, that is, in this way, the patient's own anti-tumor immune response can be activated.

[0032] More importantly, under the action of external electromagnetic force, the tumor cells of the target object can sense the dipole moment, forming a potential difference around the cell tissue, that is, forming an electric field inside the biological tissue. The internal electric field can act on the tubulin of the proliferating cancer cells in the late stage of cell division, and can inhibit tumor growth and cause the cancer cells affected by the electric field to die by interfering with the mitosis of cancer cells. The electric field can selectively interfere with cancer cells in the rapid proliferation and division stage that produce highly charged substances, causing them to enter the cell suicide programmed death, while having no obvious effect on normal human cells in the quiescent and division stages.

[0033] The arc contacts b at both ends of the notch a at the end of the frame structure 40 may be spherical structures to match the size of the frame structure 40, so that the surface of the frame structure 40 has a certain curvature, thereby reducing the risk of high-voltage tip discharge.

[0034] The seat mechanism can be a lifting chair, so that the target object can adjust its position so that the area to be treated is aligned with the transmitting area and the receiving area, avoiding other parts from receiving electromagnetic wave radiation.

[0035] In this embodiment, the transmitting device 2 and the receiving device 3 are both provided with an insulating shell, and a roller, such as a universal wheel, is provided at the bottom of the insulating shell.

[0036] Furthermore, the insulating housing is provided with indicator plates 45 in the areas corresponding to the first electrode 41 and the second electrode 42, respectively, so as to align the user's treated part with the areas where the first electrode 41 and the second electrode 42 are located. Preferably, the indicator plates 45 are circular or polygonal.

[0037] A first support frame 5 is arranged in the insulating shell of the transmitting device 2, and the boosting structure 21 is arranged on the first support frame 5; and / or a second support frame 6 is arranged in the insulating shell of the receiving device 3, and the voltage transformation structure 31 is arranged on the second support frame 6.

[0038] Specifically, the first support frame 5 and the second support frame 6 each include: a bottom plate, a plurality of support columns and a top plate; wherein each support column is connected between the bottom plate and the top plate, the boost structure 21 is arranged on the top plate of the first support frame 5, and the first electrode 41 is connected to the output end of the boost structure 21; the transformer structure 31 is arranged on the top plate of the second support frame 6, and the second electrode 42 is connected to the output end of the transformer structure 31. The top plate may be in a convex shape, and a support is arranged on the top plate to support the boost structure 21 or the transformer structure 31. The top of the support may be an arc-shaped groove to fit with the outer wall of the boost structure 21.

[0039] In specific implementation, the boost structure 21 is arranged in the horizontal direction, and the first electrode 41 is arranged at the output end of the boost structure 21 in the vertical direction; the transformer structure 31 is arranged opposite to the boost structure 21 in the horizontal direction, and the second electrode 42 is arranged at the output end of the transformer structure 31 in the vertical direction.

[0040] In this embodiment, the boost structure 21 and the transformer structure 31 are both Tesla coils, and the frequencies of the two Tesla coils are the same.

[0041] Since when a Tesla coil is working, an alternating magnetic field is created in the surrounding space. If another Tesla coil is located within the effective range of this alternating magnetic field and its resonant frequency matches or is close to that of the transmitting coil, the conductor in the receiving coil will feel the changing magnetic field, thereby inducing current and voltage inside it.

[0042] In this embodiment, the boost structure 21 can boost the medium frequency low voltage AC signal to above 100 kV, and the second electrode 42 connected to the transformer structure 31 can generate an induced voltage of not less than 50 kV. Due to the large potential difference, a high field strength electric field is formed between the transmitting device 2 and the receiving device 3.

[0043] In this embodiment, the AC signal generating device 1 can be any device in the prior art, for example, the AC signal generating device 1 is provided with a plug for connecting to 220V AC. The AC signal generating device 1 in the embodiment of the present invention can include: a single-chip control circuit and a touch screen; wherein the single-chip control circuit is connected to the touch screen to receive a command signal from the touch screen to adjust the current and voltage in the circuit to generate a medium-frequency low-voltage AC signal.

[0044] In actual use, the parameters of the output core electric field signal can be controlled through the touch screen. The main core parameters include: electric field waveform, electric field main frequency, pulse width, pulse number and time, etc. According to different tumor types and imaging data, the parameters can be fine-tuned through touch screen control, so that the AC signal generating device 1 outputs medium frequency electric field signals of different frequencies for transmission to the transmitting device 2.

[0045] It can be obviously concluded from the above that the tumor treatment device provided in this embodiment boosts the medium-frequency low-voltage AC signal to a first voltage signal with a higher voltage value through the boost structure 21 in the transmitting device 2, and loads it to the first electrode 41. At the same time, the transformer structure 31 in the receiving device 3 cooperates with the boost structure 21 to generate an induced voltage with a voltage value lower than the first voltage signal, and loads it to the second electrode 42. The second electrode 42 couples with the first electrode 41 and emits electromagnetic wave signals of multiple frequencies, so that the target object receives the electromagnetic wave radiation at the optimal position, thereby inhibiting the mitosis of its tumor tissue and cells. The first electrode 41 and the second electrode 42 do not need to contact the target object, which optimizes the user experience of the target object and can generate a higher field strength electric field and multi-frequency electromagnetic waves, greatly enhancing the inhibition rate of tumor cells.

[0046] Continue reading Figure 3 In the above embodiment, the frame structure 40 is a ring frame, and the ring frame is multiple, and the multiple ring frames are concentrically arranged to form a concentric circle structure, and the contact connection lines at both ends of each notch a are arranged at an angle c.

[0047] Specifically, in the concentric circle structure, the annular frames may be arranged at equal intervals, or may be arranged in a manner in which the intervals decrease from the outer circle to the inner circle.

[0048] In a specific implementation of this embodiment, in the concentric circle structure, the diameters of the metal tubes constituting each of the annular frames are equal.

[0049] Preferably, the diameter of the metal tube constituting the annular frame decreases from the outermost circle to the innermost circle of the concentric circle structure, which is beneficial to reducing the risk of air discharge effect generated by the first electrode 41 and the second electrode 42 .

[0050] In a specific implementation of the embodiment of the present invention, the notches a of the annular frames are arranged in an array along the same direction.

[0051] Preferably, the contact lines at both ends of each notch a are arranged at an angle of 20-40°, preferably 34°, which can make the radiation range of electromagnetic waves larger and is conducive to improving the overall gain of the array structure formed by each notch a.

[0052] In another specific implementation of the embodiment of the present invention, the notches a of two adjacent annular frames are arranged in opposite directions and are located opposite to each other.

[0053] Furthermore, a first fastener 43 is provided on the frame structure of the first electrode 41 for connecting with the transmitting device 2 ; and / or a second fastener 44 is provided on the frame structure of the second electrode 42 for connecting with the receiving device 3 .

[0054] In a specific implementation, the first fastener 43 and the second fastener 44 are both made of insulating materials, such as polyoxymethylene materials. The first fastener 43 and the second fastener 44 have the same structure.

[0055] More specifically, the first fastener 43 and the second fastener 44 each include: at least two intersecting connecting plates 400; wherein each of the connecting plates 400 is provided with a plurality of corresponding slots 4001 or connecting holes for fixing to the corresponding frame structure 40 respectively.

[0056] Specifically, the connecting plate 400 may be a strip plate with arc-shaped ends, and any two adjacent connecting plates 400 are arranged at a preset angle. For example, when there are two connecting plates 400, the two connecting plates 400 are arranged vertically.

[0057] The diameter of each slot 4001 on the connecting plate 400 matches the thickness of each frame structure 40 to ensure that each frame structure 40 is firmly connected to the slot 4001. Each slot 4001 is arranged in a one-to-one correspondence with each frame structure 40, that is, the number of slots is consistent with the number of frame structures 40.

[0058] Of course, a plurality of connection holes (not shown in the figure) may be provided on the connection plate 400, and the connection holes on each connection plate 400 are provided in pairs, so as to sequentially pass connection belts (not shown in the figure) through the connection plates 400, thereby fixing each frame structure 40 on the connection plate 400. For example, a pair of connection holes are symmetrically provided on both sides of each frame structure 40, so that the connection belt is tied to the frame structure 40. The connection belt may be made of insulating material, such as polyoxymethylene.

[0059] In order to verify the inhibitory effect of the tumor treatment device provided in the embodiment of the present invention on tumor cells, the present invention uses the device to perform a cell growth curve test on U251 brain glioma cells, and performs apoptosis tests on three tumor cells, namely, U251 brain glioma cells, MCF7 cervical cancer cells and Hela uterine cancer cells. The test method is as follows: Experimental method 1: Cell viability assay Preparation before the experiment: Cells were plated one day before the experiment, and the growth was observed using an Olympus CKX53 fluorescent inverted microscope. U251 brain glioma cells with a confluence of 70%-80% were selected, rinsed with GIBCO Ph7.4 PBS, and digested with GIBCO 0.25% trypsin at room temperature for 90 seconds. The cells were collected and diluted with DMED complete medium to a concentration of 20,000 cells / ml. The cells were plated on COSTAR 96-well transparent cell culture plates, with 100 μl per well and 2,000 cells. There was one plate for the control group, and the number of plates for the experimental group was determined according to the experimental protocol. The cells were paralleled in 3 wells, and the blank medium control 3 wells were used as a monitoring point. The number of monitoring points per plate was designed according to the experimental requirements, and the plates were placed in a 37°C, 5% BPN-150CH (UV) carbon dioxide incubator for overnight culture.

[0060] On the day of the experiment The first test was performed 90 minutes before the start of the experiment. 10 μl of MCE CellCounting Kit-8 was added to the 6 wells of the monitoring point, and the wells were placed in a 37°C, 5% BPN-150CH (UV) carbon dioxide incubator for incubation for 90 minutes. The absorbance reading was performed using a MIULAB DEL-100 microplate reader at a 450 nm filter. The reading value was the cell empty average value minus the blank control empty average value. Each monitoring point was then treated in the same way.

[0061] During the experiment, the 96-well plate was placed in a homemade insulated thermostat at 37°C and 5% carbon dioxide between the machines for treatment. The test was performed at different time points according to the experimental requirements. The time distribution of each monitoring point was recorded as the horizontal axis, and the absorbance was recorded as the vertical axis. The growth curve was obtained using a line graph.

[0062] By comparing the growth rates of the growth curves of the experimental group and the control group, the doubling time at different time points was calculated and compared, and a one-way analysis of variance was performed at each point to obtain the P value to judge the validity of the results.

[0063] Experimental method 2: cell apoptosis detection Preparation before the experiment: Cells were plated one day before the experiment, and the growth was observed using an Olympus CKX53 fluorescence inverted microscope. U251 brain glioma cells with a confluence of 70%-80% were selected, rinsed with GIBCO Ph7.4 PBS, and digested with GIBCO 0.25% trypsin at room temperature for 90 seconds. The cells were collected and diluted to a concentration of 40,000 cells / ml using DMED complete medium from Pronose. The cells were plated on a COSTAR 96-well white cell culture plate, with 50 μl per well and 2,000 cells. One plate for the control group, and the number of plates for the experimental group was determined according to the experimental protocol. Three parallel wells of cells were plated, and three wells of blank culture medium were used as controls. The cells were placed in a 37°C, 5% BPN-150CH (UV) carbon dioxide incubator. At 19:00-20:00 in the evening, 50 μl of the prepared RealTime-Glo™ Annexin V Apoptosis Assay 2X working solution was added, mixed well, and returned to the incubator. On the day of the experiment: Before the experiment, the PROMEGA GLOMAX DISCOVER multi-function microplate reader was used to read the luminescence. The single-well reading time was 0.3s, and the cell empty average value minus the blank control empty average value was the reading value. During the experiment, the reading was taken every 2 hours, and the time distribution of each monitoring point was recorded as the horizontal axis, and the luminescence reading was recorded as the vertical axis. The line graph was used to obtain the cell apoptosis kinetic curve.

[0064] The experimental results were determined by comparing the kinetics and numerical values ​​of the apoptosis curves of the experimental group and the control group.

[0065] In addition, the test method for MCF7 breast cancer cells and HELA cervical cancer cells is the same as the above-mentioned cell apoptosis detection test.

[0066] The results of the above cell viability and apoptosis assays are shown in Figure 6-9 As shown, through Figure 6 It can be seen that the U251 glioma cells after electric field radiation grow slower than the cells in the incubator. Figure 6 Middle: L1 is the growth curve of U251 glioma cells after being treated with the non-invasive tumor treatment device provided by the embodiment of the present invention, and L2 is the growth curve of U251 glioma cells in a normal incubator.

[0067] pass Figure 7 It can be seen that the U251 glioma cells subjected to electric field radiation have a more obvious apoptosis trend than the cells in the incubator. Figure 7 Middle: L3 is the apoptosis curve of U251 glioma cells treated with the non-invasive tumor treatment device provided by the embodiment of the present invention, and L4 is the apoptosis curve of U251 glioma cells in a normal incubator.

[0068] Likewise, through Figure 8-9 It can be clearly seen that the electric field generated by the device provided by the present invention can induce apoptosis of MCF7 breast cancer cells and HELA cells. Figure 8 Middle: L5 is the apoptosis curve of MCF7 breast cancer cells treated with the non-invasive tumor treatment device provided by the embodiment of the present invention, and L6 is the apoptosis curve of MCF7 breast cancer cells in a normal incubator; Fig. 9 Middle: L7 is the apoptosis curve of HELA cervical cancer cells treated with the non-invasive tumor treatment device provided by the embodiment of the present invention, and L8 is the apoptosis curve of HELA cervical cancer cells in a normal incubator.

[0069] Through the above cell viability experiment and apoptosis detection test, it is proved that the electric field generated by the device provided by the present invention has an inhibitory effect on tumor cells and can promote tumor cell apoptosis. Compared with the prior art, the present invention has the following advantages: (1) It is completely a physical therapy, non-invasive and has no side effects; (2) It is a non-contact treatment, will not cause discomfort to the patient and is very convenient to operate.

[0070] In summary, the tumor treatment device provided in this embodiment boosts the medium-frequency low-voltage AC signal to a first voltage signal with a higher voltage value through the boost structure in the transmitting device, and loads it to the first electrode. At the same time, the transformer structure and the boost structure in the receiving device cooperate to generate an induced voltage with a voltage value lower than the first voltage signal, and load it to the second electrode. The second electrode is coupled to the first electrode and emits electromagnetic wave signals of multiple frequencies, so that the target object receives the electromagnetic wave radiation at the optimal position, thereby inhibiting the mitosis of its tumor tissue and cells.

[0071] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A tumor treatment device, characterized in that: include: AC signal generating device, transmitting device and receiving device; wherein, The AC signal generating device is connected to the transmitting device, and is used to generate a medium-frequency low-voltage AC signal and transmit the medium-frequency low-voltage AC signal to the transmitting device; The transmitting device is provided with a boost structure for converting the medium-frequency low-voltage AC power signal into a first voltage signal; the voltage value of the first voltage signal is greater than the voltage value of the medium-frequency low-voltage AC power signal, and the output end of the boost structure is connected to a first electrode for receiving the first voltage signal loaded by the boost structure; The receiving device is arranged opposite to the transmitting device and connected to the AC signal generating device. A transformer structure is arranged in the receiving device for matching with the boost structure to generate an induced voltage signal. The voltage value of the induced voltage signal is smaller than the voltage value of the first voltage signal, thereby generating an electric field with high field strength between the transmitting device and the receiving device. The output end of the transformer structure is connected to a second electrode, the second electrode is used to receive the induced voltage signal loaded by the transformer structure and couple with the first electrode to emit electromagnetic wave signals of multiple frequencies; The first electrode and the second electrode both have a frame structure, a notch is provided at the end of the frame structure, and arc-shaped contacts are provided at both ends of the notch to prevent arc discharge; A seat mechanism is arranged in the area between the receiving device and the transmitting device, so as to enable the target object to receive high-field-strength and multi-frequency electromagnetic wave radiation at an optimal position.

2. The tumor treatment device according to claim 1, characterized in that: The frame structure is a ring frame, and there are multiple ring frames. The multiple ring frames are concentrically arranged to form a concentric circle structure, and the contact connection lines at both ends of each notch are arranged at an angle.

3. The tumor treatment device according to claim 1, characterized in that: A first fastener is provided on the frame structure of the first electrode for connecting with the transmitting device; and / or a second fastener is provided on the frame structure of the second electrode for connecting with the receiving device.

4. The tumor treatment device according to claim 3, characterized in that: The first fastener and the second fastener each include: at least two intersecting connecting plates; wherein, Each of the connecting plates is provided with a plurality of slots or connecting holes corresponding to each other, so as to be fixed to the corresponding frame structure respectively.

5. The tumor treatment device according to claim 1, characterized in that: The first electrode is electrically connected to the output end of the boost structure through a first high-voltage wire; and / or the second electrode is electrically connected to the output end of the transformer structure through a second high-voltage wire.

6. The tumor treatment device according to claim 1, characterized in that: The boost structure and the transformer structure are both Tesla coils, and the frequencies of the two Tesla coils are the same.

7. The tumor treatment device according to claim 1, characterized in that: The transmitting device and the receiving device are both provided with an insulating shell, and a roller is provided at the bottom of the insulating shell.

8. The tumor treatment device according to claim 7, characterized in that: Indicator plates are respectively provided on the insulating shell in areas corresponding to the first electrode and the second electrode, so as to align the user's part to be treated with the areas where the first electrode and the second electrode are located.

9. The tumor treatment device according to claim 8, characterized in that: The indicating plate is circular or polygonal.

10. The tumor treatment device according to claim 7, characterized in that: A first support frame is arranged in the insulating shell of the transmitting device, and the boost structure is arranged on the first support frame; and / or a second support frame is arranged in the insulating shell of the receiving device, and the voltage transformation structure is arranged on the second support frame.

Images