A composition containing a penk protein and its use

By providing recombinant enkephalinogenase PENK protein to interact with CBFB protein and bind to BMP2 protein, osteogenic differentiation of odontogenic cells is promoted, solving the unknown role of PENK in tooth development, achieving significant osteogenic differentiation effect, and providing a means of detecting osteogenic markers.

CN120209114BActive Publication Date: 2026-04-07CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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Patent Information

Application Number
CN202510267415.3
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-03-07
Publication Date
2026-04-07
Estimated Expiration
2045-03-07

AI Technical Summary

Technical Problem

In the existing technology, the role of PENK in tooth development has not been reported, and its key role in the development of periodontal soft and hard tissues has not been fully recognized. There is a lack of effective means to promote osteogenic differentiation of dental mesenchymal cells and embryonic jawbone bone marrow stromal stem cells.

Method used

The study provides a recombinant proenkephalin PENK protein and its encoding gene, which promotes osteogenic differentiation by interacting with CBFB protein, enhances osteogenic effects by combining with BMP2 protein, and inhibits osteogenic differentiation by interfering with PENK expression via siRNA.

Benefits of technology

It significantly enhances the osteogenic differentiation capacity of dental mesenchymal cells, embryonic jawbone bone marrow stromal stem cells, and embryonic long bone bone marrow mesenchymal cells. The combined use of PENK recombinant protein and BMP2 shows excellent results, while interference with PENK expression inhibits osteogenic differentiation, providing an effective method for detecting osteogenic markers.

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Abstract

This application provides a composition containing PENK protein and its application in promoting osteoblast osteogenic differentiation. Compared with the prior art, this application has the following advantages: The pro-PENK recombinant protein and the PENK-containing composition provided in this application, after being applied to cells with osteogenic potential, enhance osteogenic performance through interaction with CBFB protein, and can significantly promote osteogenic differentiation. Compared with the existing bone morphogenetic protein 2 (BMP recombinant protein), the osteogenic effect is superior. Furthermore, experiments have verified that the osteogenic differentiation effect of pro-PENK recombinant protein on osteoblasts is dose-dependent. This application further verifies that the combined use of low doses of PENK recombinant protein and BMP recombinant protein has a more significant cellular osteogenic effect than the use of either at high doses alone.
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