A peroxidase and its use

The peroxidase POD-8, produced through gene synthesis and recombinant expression, solves the problems of low catalytic efficiency and poor stability of natural enzymes, and achieves efficient degradation of phenolic compounds and dyes, especially efficient decolorization of 2,6-DMP and various dyes, making it suitable for industrial wastewater treatment.

CN120574797BActive Publication Date: 2025-10-17HANGZHOU LUCA INTELLIGENT TECHNOLOGY CO LTD
4 Cites 0 Cited by

Patent Information

Application Number
CN202511006888.4
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-07-22
Publication Date
2025-10-17
Estimated Expiration
2045-07-22

AI Technical Summary

Technical Problem

Existing natural peroxidases (such as horseradish peroxidase HRP) have low catalytic efficiency and poor stability, making it difficult to effectively degrade phenolic compounds. They are also easily inactivated in industrial wastewater, and traditional chemical oxidation methods pose a risk of secondary pollution.

Method used

Peroxidase POD-8 was obtained through gene synthesis and recombinant expression. Its protein structure was predicted using AlphaFold and expressed in Escherichia coli. It was purified using a His-SUMO tag. The enzyme activity and stability towards different substrates, the optimal pH and temperature, and the metal ion concentration were explored for application in dye wastewater treatment.

Benefits of technology

POD-8 has high enzyme activity and stability towards 2,6-DMP, is tolerant to a variety of metal ions, and can effectively decolorize a variety of dyes, especially Congo red and amino black 10B, with a decolorization rate of up to 25%, showing excellent application prospects in dye wastewater treatment.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The application discloses a peroxidase and application thereof, and relates to the field of bioengineering.The peroxidase POD-8 provided by the application has good enzyme activity of oxidizing phenolic substrates, especially the highest enzyme activity on 2,6-DMP, and has very good persistence, can maintain stable enzyme activity within 10 min, and has relatively strong tolerance to various metal ions, can still maintain more than 90 % of enzyme activity in the presence of various metal ions with various concentrations, has relatively strong decolorizing capacity on various dyes, especially the decolorizing efficiency on Congo red and amino black 10B is as high as about 25 % within 24 h, and has a good application prospect in dye wastewater treatment.
Need to check novelty before this filing date? Find Prior Art