Composite yolk immunoglobulin antibody toothpaste and preparation method thereof

Abstract

The invention discloses composite yolk immunoglobulin antibody toothpaste and a preparation method thereof, and the toothpaste comprises the following components in percentage by mass: 0.01-1.0% of yolk immunoglobulin; 10-70% of a humectant; 0.01%-0.5% of an acid-base buffer agent; 0.01%-0.5% of a taste improver; 0.1%-3% of a thickening agent; 5-50% of a friction agent; 0.1-5% of a foaming agent; 0.01%-0.5% of an appearance improver; 0.1 to 2.0 percent of essence; and the balance of water. Wherein the egg yolk immune globulin is a composite specific egg yolk antibody for resisting porphyromonas gingivalis, fusobacterium nucleatum and prevotella intermedia. Starting from three key pathogenic bacteria causing periodontal diseases, the egg yolk composite antibody resisting porphyromonas gingivalis and having specificity of fusobacterium nucleatum and prevotella intermedius is added into a toothpaste formula, and the effects of inhibiting bacteria, preventing and relieving oral inflammation, repairing gingiva and the like are achieved in a tooth brushing mode.

Description

Technical Field

[0001] The invention relates to the technical field of toothpaste formulations, in particular to a composite egg yolk immunoglobulin antibody toothpaste and a preparation method thereof. Background Art

[0002] Periodontitis is a chronic infectious disease caused by dental plaque microorganisms that primarily affects tooth-supporting tissues such as the gums, periodontal ligament, and alveolar bone. Clinically, it is characterized by periodontal pocket formation, alveolar bone resorption, and tooth mobility. Gram-negative anaerobic bacteria such as Porphyromonas gingivalis (Pg), Fusobacterium nucleatum (Fn), and Prevotella intermedia (Pi), which colonize the dental plaque biofilm, are the primary pathogens. These microorganisms directly damage periodontal tissues by secreting toxic substances such as endotoxins and proteases, while also activating the host immune response to produce excessive inflammatory mediators, leading to connective tissue destruction and bone resorption. Furthermore, poor restorations, vitamin deficiencies, and poor oral hygiene practices can synergistically exacerbate the condition.

[0003] Current antibiotic adjunctive therapies pose challenges such as bacterial imbalance and the risk of drug resistance due to broad-spectrum antimicrobial activity, making precise treatment difficult. Passive immunization technology has attracted attention due to its highly targeted and safe properties. Therefore, developing a toothpaste formula that combines passive immunization technology to achieve antibacterial effects, prevent and alleviate oral inflammation, and repair gums is a pressing technical challenge for those skilled in the art. Summary of the Invention

[0004] The purpose of the present invention is to provide a composite egg yolk immunoglobulin antibody toothpaste and a preparation method thereof in view of the deficiencies in the prior art.

[0005] To achieve the above object, the technical solution adopted by the present invention is:

[0006] The first aspect is to provide a composite egg yolk immunoglobulin antibody toothpaste, which comprises, by mass percentage: 0.01-1.0% egg yolk immunoglobulin; 10-70% moisturizer; 0.01-0.5% acid-base buffer; 0.01-0.5% taste improver; 0.1-3% thickener; 5-50% abrasive; 0.1-5% foaming agent; 0.01-0.5% appearance improver; 0.1-2.0% flavor; the balance being water;

[0007] The egg yolk immunoglobulin is a composite specific egg yolk antibody against Porphyromonas gingivalis, Fusobacterium nucleatum and Prevotella intermedia.

[0008] Furthermore, the composite egg yolk immunoglobulin antibody toothpaste comprises, by mass percentage: 0.01-0.20% egg yolk immunoglobulin; 30-70% moisturizer; 0.1-0.5% acid-base buffer; 0.1-0.4% taste improver; 0.5-2.0% thickener; 10-40% abrasive; 1-4% foaming agent; 0.1-0.5% appearance improver; 0.5-1.5% flavor; and the balance is water.

[0009] Furthermore, the moisturizing agent is selected from one or more of sorbitol, glycerin, and polyethylene glycol-32.

[0010] Furthermore, the acid-base buffer is selected from one or more of trisodium phosphate, tetrasodium pyrophosphate, and sodium dihydrogen phosphate.

[0011] Furthermore, the taste improver is selected from one or more of trichlorogalactose, sodium saccharin, and xylitol.

[0012] Furthermore, the thickener is selected from one or more of cellulose gum, carrageenan, hydroxyethyl cellulose, and xanthan gum.

[0013] Furthermore, the friction agent is selected from one or more of hydrated silica, calcium carbonate, hydroxyapatite, and calcium hydrogen phosphate.

[0014] Furthermore, the foaming agent is selected from one or more of sodium lauryl sulfate, cocamide DEA, sodium lauroyl sarcosinate, and cocamidopropyl betaine.

[0015] Furthermore, the appearance improver is CI77891.

[0016] The second aspect is to provide a method for preparing the above-mentioned composite egg yolk immunoglobulin antibody toothpaste, comprising the following steps:

[0017] Step 1: Pour some moisturizer into the ointment pot;

[0018] Step 2: Add the acid-base buffer, taste modifier and the remaining moisturizer to water, stir at 40-50°C for 10-20 minutes until completely dissolved, and then pour into the paste-making pot;

[0019] Step 3: Mix the thickener and the friction agent, then pour into the paste making pot, evacuate to a vacuum of ≤-0.094 MPa, and stir for 10 to 20 minutes;

[0020] Step 4: Add a foaming agent, evacuate to a vacuum of ≤-0.094 MPa, and stir for 10 to 20 minutes;

[0021] Step 5: add egg yolk immunoglobulin, evacuate to a vacuum of ≤-0.094 MPa, and stir for 5 to 10 minutes;

[0022] Step 6: Add appearance improver, evacuate to a vacuum of ≤-0.094 MPa, and stir for 5 to 10 minutes;

[0023] Step 7: Add essence, evacuate to a vacuum of ≤-0.094 MPa, and stir for 5 to 10 minutes;

[0024] Step 8: Turn off the equipment stirring, break the vacuum of the paste making pot, and take samples for inspection

[0025] Step nine: After the material is qualified, filter and discharge;

[0026] Step 10: filling, coding, and sealing to obtain the finished product.

[0027] The present invention adopts the above technical solution, which has the following technical effects compared with the prior art:

[0028] The present invention starts with three key pathogens that cause periodontal diseases, and adds "specific egg yolk complex antibodies against Porphyromonas gingivalis, Fusobacterium nucleatum and Prevotella intermedia" to the toothpaste formula. By brushing teeth, it can inhibit bacteria, prevent and reduce oral inflammation, and repair gums. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 This is a bar graph of IL-6 content in Example 6.

[0030] Figure 2 It is a bar graph of TNF-α content in Example 6.

[0031] Figure 3 This is a bar graph of IL-β content in Example 6.

[0032] Figure 4 This is a micrograph of the cell migration experiment in Example 7.

[0033] Figure 5 This is a bar graph of cell healing rate in Example 7. DETAILED DESCRIPTION

[0034] The present invention will be further described below with reference to the accompanying drawings and specific embodiments, but they are not intended to limit the present invention. It should be noted that, unless there is a conflict, the embodiments and features in the embodiments of the present invention may be combined with each other.

[0035] Example 1

[0036] This embodiment provides a method for preparing a composite egg yolk immunoglobulin antibody toothpaste, which specifically comprises the following steps:

[0037] Step 1: Pour some moisturizer into the ointment pot;

[0038] Step 2: Add the acid-base buffer, taste modifier and the remaining moisturizer to the solvent water, stir for 10-20 minutes at 40-50°C until completely dissolved, and then pour into the paste making pot;

[0039] Step 3: Mix the thickener and the friction agent, then pour into the paste making pot, evacuate to a vacuum of ≤-0.094 MPa, and stir for 10 to 20 minutes;

[0040] Step 4: Add a foaming agent, evacuate to a vacuum of ≤-0.094 MPa, and stir for 10 to 20 minutes;

[0041] Step 5: add egg yolk immunoglobulin, evacuate to a vacuum of ≤-0.094 MPa, and stir for 5 to 10 minutes;

[0042] Step 6: Add appearance improver, evacuate to a vacuum of ≤-0.094 MPa, and stir for 5 to 10 minutes;

[0043] Step 7: Add essence, evacuate to a vacuum of ≤-0.094 MPa, and stir for 5 to 10 minutes;

[0044] Step 8: Turn off the equipment stirring, break the vacuum of the paste making pot, and take samples for inspection

[0045] Step nine: After the material is qualified, filter and discharge;

[0046] Step 10: filling, coding, and sealing to obtain the finished product.

[0047] Example 2

[0048] This embodiment provides a composite egg yolk immunoglobulin antibody toothpaste, which is prepared using the method of the above-mentioned embodiment 1. The specific composition and proportion of the components in the toothpaste are shown in Table 1 below:

[0049] Table 1

[0050] Serial number name Content (w / w%) Name in Example 1 1 sorbitol 50.00 Some moisturizers 2 glycerin 1.00 Some moisturizers 3 Trisodium phosphate 0.10 Acid-base buffers 4 Tetrasodium pyrophosphate 0.10 Acid-base buffers 5 trichlorogalactose 0.10 Taste improvers 6 Saccharin sodium 0.10 Taste improvers 7 Polyethylene glycol-32 1.00 Moisturizer for the rest 8 Cellulose gum 1.00 thickener 9 Chondrus crispus 0.20 thickener 10 Hydroxyethyl cellulose 0.20 thickener 11 Xanthan gum 0.20 thickener 12 Hydrated silica 22.00 Friction agent 13 Hydroxyapatite 2.00 Friction agent 14 Sodium Lauryl Sulfate 2.50 foaming agent 15 Cocamidopropyl Betaine 0.50 foaming agent 16 Yolk immunoglobulin 0.02 Active ingredients 17 CI77891 0.30 Appearance improver 18 essence 1.00 essence water to100 solvent

[0051] The egg yolk immunoglobulin is a composite specific egg yolk antibody against Porphyromonas gingivalis, Fusobacterium nucleatum, and Prevotella intermedia. Its preparation process primarily involves inactivating Porphyromonas gingivalis (Accession Number: ATCC33277), Fusobacterium nucleatum (Accession Number: ATCC10953), and Prevotella intermedia (Accession Number: ATCC25611) and preparing them into antigens. This is then administered subcutaneously and / or intramuscularly to poultry (laying hens) to immunize them. The process then involves egg collection, egg white and yolk separation, yolk collection, acid dilution, flocculation sedimentation, ultrafiltration concentration, and freeze-drying.

[0052] Example 3

[0053] This embodiment provides a composite egg yolk immunoglobulin antibody toothpaste, which is prepared using the method of the above-mentioned embodiment 1. The specific composition and proportion of the components in the toothpaste are shown in Table 2 below:

[0054] Table 2

[0055] Serial number name Content (w / w%) Name in Example 1 1 sorbitol 30.00 Some moisturizers 2 glycerin 10.00 Some moisturizers 3 Trisodium phosphate 0.10 Acid-base buffers 4 Tetrasodium pyrophosphate 0.10 Acid-base buffers 5 Sodium dihydrogen phosphate 0.20 Acid-base buffers 6 trichlorogalactose 0.10 Taste improvers 7 Saccharin sodium 0.20 Taste improvers 8 Xylitol 0.10 Taste improvers 9 Polyethylene glycol-32 10.00 Moisturizer for the rest 10 Cellulose gum 1.00 thickener 11 Xanthan gum 0.50 thickener 12 Hydrated silica 20.00 Friction agent 13 Hydroxyapatite 5.00 Friction agent 14 Calcium hydrogen phosphate 5.00 Friction agent 15 Sodium Lauryl Sulfate 2.00 foaming agent 16 Cocamidopropyl Betaine 1.00 foaming agent 17 Yolk immunoglobulin 0.20 Active ingredients 18 CI77891 0.25 Appearance improver 19 essence 1.50 essence water to100 solvent

[0056] The egg yolk immunoglobulin is a composite specific egg yolk antibody against Porphyromonas gingivalis, Fusobacterium nucleatum, and Prevotella intermedia. Its preparation process primarily involves inactivating Porphyromonas gingivalis (Accession Number: ATCC33277), Fusobacterium nucleatum (Accession Number: ATCC10953), and Prevotella intermedia (Accession Number: ATCC25611) and preparing them into antigens. This is then administered subcutaneously and / or intramuscularly to poultry (laying hens) to immunize them. The process then involves egg collection, egg white and yolk separation, yolk collection, acid dilution, flocculation sedimentation, ultrafiltration concentration, and freeze-drying.

[0057] Example 4: Titer Test of Three IgY Antibodies in Toothpaste

[0058] This example aims to accurately determine the activity titers of IgY antibodies against three periodontal disease pathogens (Porphyromonas gingivalis, Fusobacterium nucleatum, and Prevotella intermedia) in "toothpaste containing egg yolk immunoglobulin" (Example 2) through a specific detection method to evaluate its efficacy and potential in practical applications.

[0059] This experiment uses an indirect ELISA enzyme-linked immunosorbent assay (ELISA) technique to determine the activity and titer of IgY antibodies. The principle is to immobilize antigens corresponding to three periodontal disease pathogens on a carrier to prepare a solid-phase antigen. A sample containing the target IgY antibody is then added, and the antibody and antigen specifically bind. An enzyme-labeled secondary antibody is then added and binds to the antigen-antibody complex. Finally, a substrate is added, and the substrate develops color under enzyme catalysis. The intensity of the color development is positively correlated with the IgY antibody content in the sample. The antibody titer is then calculated by measuring the absorbance (OD) value.

[0060] 4.1 Experimental Materials and Instruments

[0061] Antigens: Antigens prepared from pathogenic bacteria closely related to periodontal disease (Porphyromonas gingivalis, Fusobacterium nucleatum, Prevotella intermedia);

[0062] Test sample: the composite egg yolk immunoglobulin antibody toothpaste in Example 2;

[0063] Reagents: horseradish peroxidase-conjugated donkey anti-chicken immunoglobulin Y polyclonal antibody, coating buffer, blocking solution (bovine serum albumin), washing solution, color development solution, and stop solution;

[0064] Instruments: detachable ELISA plate, micropipette, refrigerator, ELISA reader, constant temperature box.

[0065] 4.2 Experimental steps

[0066] 1) Antigen coating: Dilute bacterial antigen to an appropriate concentration with coating diluent, add 100 μL of antigen to each well (except blank), and incubate at 4°C overnight. The next day, spin dry the antigen and wash with washing solution three times, adding 300 μL of washing solution to each well for 2 minutes each time.

[0067] 2) Blocking: Add 300 μL of blocking solution to each well of the ELISA plate and incubate at 37°C for 2 hours. Then spin dry. Add 300 μL of washing solution to each well and wash three times for 2 minutes each time.

[0068] 3) Add the sample to be tested: dilute the sample to be tested in a doubling gradient, add 100 μL to each well of the ELISA plate, incubate at 37°C for 2 hours, then spin dry, add 300 μL of washing solution to each well, and wash 3 times, 2 minutes each time.

[0069] 4) Add enzyme-labeled secondary antibody: Add qualified enzyme conjugate, 100 μL per well (except blank), incubate at 37°C for 40 minutes, then spin dry. Add 300 μL of washing solution to each well and wash three times for 2 minutes each time.

[0070] 5) Add substrate solution: add 100 μL to each well and allow color to develop for about 20 minutes in the dark to terminate the reaction.

[0071] 6) Stop the reaction: Add 100 μL of stop solution to each well and measure the results using a microplate reader within 10 minutes.

[0072] 4.3 Experimental results (shown in Table 3 below)

[0073] Table 3

[0074]

[0075]

[0076] It can be seen that three types of IgY active antibodies were detected in the "toothpaste containing egg yolk immunoglobulin", and as time goes by, the biological activity of the three IgY antibodies in the toothpaste does not decrease significantly, indicating that IgY has excellent stability in toothpaste.

[0077] Example 5

[0078] Bad breath results from the decomposition of sulfur-containing amino acids by oral microorganisms (primarily Gram-negative anaerobes). VSCs in oral gas are closely related to the formation of bad breath. Porphyromonas gingivalis, Prevotella intermedia, and Fusobacterium nucleatum are all Gram-negative anaerobes that can use bacterial substrates to generate VSCs. This example conducts an antibacterial test on the odor-causing bacteria Porphyromonas gingivalis to evaluate the antibacterial effect of the test substance on the odor-causing bacteria, which serves as a reference indicator for evaluating the in vitro efficacy of freshening breath and reducing bad breath. The test method refers to T / COCIA18-22 "Evaluation Method for the Antibacterial Effect of Oral Cleansing and Care Products".

[0079] The antibacterial effect of the toothpaste sample (Example 2) is shown in Table 4. The results show that under the experimental conditions, the toothpaste sample has an average antibacterial rate of 76.88 for Porphyromonas gingivalis at an action concentration of 1:4 for 3 minutes, indicating that it has an antibacterial effect on Porphyromonas gingivalis and has a certain effect of freshening breath and reducing bad breath.

[0080] Table 4

[0081]

[0082] Example 6 Evaluation of the Effect of Toothpaste in Inhibiting Oral Inflammation

[0083] This example uses ELISA enzyme-linked immunosorbent assay to study the secretion of inflammatory factors such as interleukin-6 (IL-6), tumor necrosis factor α (TNF-α), and interleukin-1β (IL-1β) by gingival fibroblasts stimulated by LPS lipopolysaccharide. The effect of the egg yolk immunoglobulin toothpaste sample (Example 2) on inhibiting inflammation is evaluated by detecting changes in the content of inflammatory factors secreted by cells.

[0084] The specific test methods are as follows:

[0085] 1) Cell seeding: 1×10 5 Cells were seeded into 24-well plates at a seeding density of 100 cells / well and incubated overnight in an incubator (37°C, 5% CO2);

[0086] 2) Solution preparation: Based on the cytotoxicity test results, select three sample concentrations: 0.0313%, 0.0625%, and 0.1250%, and prepare the test substance working solution according to the experimental design Table 5;

[0087] Table 5

[0088]

[0089] 3) Drug administration: According to the experimental design table, when the cell plating rate in the 24-well plate reaches 40% to 60%, drug administration is carried out in groups, with three replicate wells per group;

[0090] 4) LPS stimulation: according to the experimental design, 100 μL of 10x LPS working solution was added to the dosed hole plate, the hole plate was shaken left and right to mix the drug in the hole plate, the final concentration of LPS was 1 μg / mL, and the incubator (37°C, 5% CO2) was continued to culture for 24 h;

[0091] 5) Sample collection: after incubation, the cell culture supernatant was collected in an EP tube (note: the amount of sample collected is determined according to the detection index), and the sample was stored in a-80°C refrigerator after collection;

[0092] 6) IL-6 content detection: according to the operation instruction of Human IL-6 ELISA kit for detection;

[0093] TNF-α content detection: according to the operation instruction of Human TNF-α ELISA kit for detection;

[0094] IL-1β content detection: according to the operation instruction of Human IL-1β ELISA kit for detection.

[0095] 6.1 IL-6 content test results (the test results are shown in Table 6, and the change trend is shown in Figure 1

[0096] Table 6

[0097]

[0098]

[0099] Compared with the BC group, the secretion amount of IL-6, an inflammatory factor of gingival fibroblasts, in the NC group was significantly increased (p<0.01), indicating that the LPS stimulation modeling in this experiment was successful.

[0100] Compared with the NC group, the secretion amount of IL-6, an inflammatory factor of gingival fibroblasts, in the PC group was significantly reduced (p<0.01) under the drug concentration of dexamethasone 100 μg / mL, indicating that the positive control detection in this experiment was effective.

[0101] Compared with the NC group, the secretion amount of IL-6, an inflammatory factor of gingival fibroblasts, in the toothpaste sample containing yolk immunoglobulin was significantly reduced (p<0.01) under the concentration of 0.0313%, 0.0625% and 0.1250% (m / V).

[0102] 6.2 TNF-α content test results (the test results are shown in Table 7, and the change trend is shown in Figure 2

[0103] Table 7

[0104] ​​

[0105]

[0106] Compared with the BC group, the secretion of inflammatory factor TNF-α in gingival fibroblasts in the NC group was significantly increased (p<0.01), indicating that the LPS stimulation conditions in this experiment were effective.

[0107] Compared with the NC group, the secretion of inflammatory factor TNF-α of gingival fibroblasts in the PC group was significantly reduced at a dexamethasone concentration of 100 μg / mL (p<0.01), indicating that the positive control test was effective.

[0108] Compared with the NC group, the toothpaste samples containing egg yolk immunoglobulin at concentrations of 0.0313%, 0.0625% and 0.1250% (m / V) significantly reduced the secretion of inflammatory factor TNF-α by gingival fibroblasts (p<0.01).

[0109] 6.3 IL-β content test results (test results are shown in Table 8, the change trend is as follows Figure 3 shown)

[0110] Table 8

[0111]

[0112] Compared with the BC group, the secretion of inflammatory factor IL-1β in gingival fibroblasts in the NC group was significantly increased (p<0.01), indicating that the LPS stimulation conditions in this experiment were effective.

[0113] Compared with the NC group, the secretion of inflammatory factor IL-1β in gingival fibroblasts in the PC group was significantly reduced at a dexamethasone concentration of 100 μg / mL (p<0.01), indicating that the positive control test was effective.

[0114] Compared with the NC group, the toothpaste samples containing egg yolk immunoglobulin at concentrations of 0.0313%, 0.0625% and 0.1250% (m / V) significantly reduced the secretion of inflammatory factor IL-1β by gingival fibroblasts.

[0115] In summary, based on the LPS-stimulated gingival fibroblast model, the secretion of inflammatory factors interleukin-6 (IL-6), tumor necrosis factor α (TNF-α) and interleukin-1β (IL-1β) in gingival fibroblasts of toothpaste samples containing egg yolk immunoglobulin at dosage concentrations of 0.0313%, 0.0625% and 0.1250% (m / V) were significantly reduced, and there was a statistical difference compared with the NC group (p<0.01), indicating that toothpaste samples containing egg yolk immunoglobulin can inhibit the secretion of inflammatory factors IL-6, TNF-α and IL-1β in gingival fibroblasts, and have a certain effect in inhibiting oral inflammation.

[0116] Example 7 Evaluation of the effect of promoting healing of gingival fibroblasts

[0117] Promoting cell proliferation and migration is a major measure for repairing gingival damage. This example uses gingival fibroblasts as the research object, and evaluates the gingival protection effect of the sample by detecting the effect of the test sample (Example 2) on the migration of gingival fibroblasts.

[0118] 7.1 Specific test methods

[0119] 1) Collect cells in logarithmic growth phase and set the cell density at 1×10 5 Inoculate cells / well into 24-well culture plates;

[0120] 2) After culturing in an incubator (37°C, 5% CO2) for 24 h, a 200 μL pipette tip was used to scratch a 24-well plate. The cells were washed three times with PBS to remove the scratched cells.

[0121] 3) Place in an incubator (37°C, 5% CO2) and culture for 24 hours. Set up three replicates for each group. The specific experimental group settings are shown in Table 9.

[0122] Table 9

[0123]

[0124] 4) Use an inverted microscope to photograph the migrated cells of each group, and use Image Pro Plus software to calculate the average scratch area.

[0125] 7.2 Test results (see Table 10 and Figure 4-5 )

[0126] Table 10

[0127]

[0128] In the cell migration assay, the cell healing level in the PC group was significantly higher than that in the BC group (p<0.01), indicating that the positive control assay was effective.

[0129] Compared with the BC group, the cell healing rate was significantly increased after treatment with samples at concentrations of 0.0313%, 0.0625%, and 0.1250% (m / V) (p<0.05).

[0130] Based on the gingival fibroblast model, the cell healing rate of human fibroblasts was significantly improved after 24 hours of treatment with toothpaste containing egg yolk immunoglobulin at concentrations of 0.0313%, 0.0625%, and 0.1250% (m / V), and there was a statistical difference compared with the BC group (p<0.05), indicating that the toothpaste containing egg yolk immunoglobulin can promote the cell healing of gingival fibroblasts and has the effect of repairing gums.

[0131] The above description is only a preferred embodiment of the present invention and does not limit the implementation mode and protection scope of the present invention. For those skilled in the art, it should be aware that all solutions obtained by equivalent substitutions and obvious changes made using the contents of the description and illustrations of the present invention should be included in the protection scope of the present invention.

Claims

1. A composite egg yolk immunoglobulin antibody toothpaste, characterized in that: Calculated by mass percentage, it includes: egg yolk immunoglobulin 0.01-1.0%; moisturizer 10-70%; acid-base buffer 0.01-0.5%; taste improver 0.01-0.5%; thickener 0.1-3%; friction agent 5-50%; foaming agent 0.1-5%; appearance improver 0.01-0.5%; essence 0.1-2.0%; balance water; The egg yolk immunoglobulin is a composite specific egg yolk antibody against Porphyromonas gingivalis, Fusobacterium nucleatum and Prevotella intermedia.

2. The composite egg yolk immunoglobulin antibody toothpaste according to claim 1, characterized in that The moisturizing agent is selected from one or more of sorbitol, glycerin, and polyethylene glycol-32.

3. The composite egg yolk immunoglobulin antibody toothpaste according to claim 1, characterized in that: The acid-base buffer is selected from one or more of trisodium phosphate, tetrasodium pyrophosphate, and sodium dihydrogen phosphate.

4. The composite egg yolk immunoglobulin antibody toothpaste according to claim 1, characterized in that The taste improver is selected from one or more of trichlorogalactose, sodium saccharin and xylitol.

5. The composite egg yolk immunoglobulin antibody toothpaste according to claim 1, characterized in that: The thickener is selected from one or more of cellulose gum, carrageenan, hydroxyethyl cellulose, and xanthan gum.

6. The composite egg yolk immunoglobulin antibody toothpaste according to claim 1, characterized in that The friction agent is selected from one or more of hydrated silica, calcium carbonate, hydroxyapatite and calcium hydrogen phosphate.

7. The composite egg yolk immunoglobulin antibody toothpaste according to claim 1, characterized in that: The foaming agent is selected from one or more of sodium lauryl sulfate, cocamide DEA, sodium lauroyl sarcosinate, and cocamidopropyl betaine.

8. The composite egg yolk immunoglobulin antibody toothpaste according to claim 1, characterized in that: The appearance improver is CI77891.

9. The method for preparing the composite egg yolk immunoglobulin antibody toothpaste according to any one of claims 1 to 8, characterized in that: The steps include: Step 1: Pour some moisturizer into the ointment pot; Step 2: Add the acid-base buffer, taste modifier and the remaining moisturizer to water, stir at 40-50°C for 10-20 minutes until completely dissolved, and then pour into the paste-making pot; Step 3: Mix the thickener and the friction agent, then pour into the paste making pot, evacuate to a vacuum of ≤-0.094 MPa, and stir for 10 to 20 minutes; Step 4: Add a foaming agent, evacuate to a vacuum of ≤-0.094 MPa, and stir for 10 to 20 minutes; Step 5: add egg yolk immunoglobulin, evacuate to a vacuum of ≤-0.094 MPa, and stir for 5 to 10 minutes; Step 6: Add appearance improver, evacuate to a vacuum of ≤-0.094 MPa, and stir for 5 to 10 minutes; Step 7: Add essence, evacuate to a vacuum of ≤-0.094 MPa, and stir for 5 to 10 minutes; Step 8: Turn off the equipment stirring, break the vacuum of the paste making pot, and take samples for inspection Step nine: After the material is qualified, filter and discharge; Step 10: filling, coding, and sealing to obtain the finished product.

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