Culture medium for inducing neural stem cells and application thereof

By using a combination of neuroectoderm and neural stem cell induction culture medium in stages with cell purification technology, the problems of uneven differentiation and low purity of neural stem cells in existing technologies have been solved, and the preparation and large-scale production of high-purity neural stem cells have been realized.

CN121343904APending Publication Date: 2026-01-16GUANGZHOU UBIGENE BIOSCIENCES CO LTD
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Patent Information

Application Number
CN202511444632.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-10-10
Publication Date
2026-01-16

AI Technical Summary

Technical Problem

Existing monolayer adhesion induction methods based on small molecules or growth factors suffer from uneven differentiation and low purity when inducing neural stem cells, making it difficult to achieve the batch acquisition and large-scale production of high-purity neural stem cells.

Method used

A phased culture medium protocol was adopted, using neuroectodermal induction medium and neural stem cell induction medium, which consist of basal medium, N-2 additive, B-27 additive, inhibitor SB431542 and Dorsomorphin, respectively. The cell purity and homogeneity were ensured by culturing in stages and purifying by picking neural rosette-shaped cell clusters. This reduced the use of factors and the number of passages.

Benefits of technology

This method enables the acquisition of high-purity (≥90%) neural stem cells, reduces the use of factors and the number of passages, improves the uniformity and purity of differentiation, and lowers the preparation cost.

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Abstract

The invention relates to the technical field of cell biology, in particular to a culture medium for inducing neural stem cells and application thereof.The culture medium for inducing the neural stem cells comprises a neural ectoderm induction culture medium and a neural stem cell induction culture medium; the neural ectoderm induction culture medium is prepared from a basic culture medium, an N-2 additive, a B-27 additive, an inhibitor SB431542 and an inhibitor Dorsomorphin, the neural stem cell induction culture medium is prepared from a basic culture medium, an N-2 additive and a B-27 additive, the basic culture medium is composed of a DMEM / F12 culture medium, a Neurobase culture medium, GlutaMax and non-essential amino acid NEAA. The problems that neural stem cells obtained through an existing single-layer adherent induction method based on small molecules or growth factors are uneven in differentiation and low in purity are solved.
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