Multi-site mutated phenylpyruvate decarboxylase mutant and application thereof in production of tyrosol
By performing multi-site mutations and genetic engineering on the phenylpyruvate decarboxylase of Saccharomyces cerevisiae, an efficient tyrosol production pathway was constructed, solving the problem of low tyrosol yield and achieving efficient tyrosol synthesis, supporting industrial production.
Patent Information
- Application Number
- CN202511715462.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-11-21
- Publication Date
- 2026-02-24
AI Technical Summary
Existing technologies yield low levels of tyrosol, and traditional extraction methods are limited by long plant growth cycles, complex extraction processes, and unstable yields, making it difficult to meet the needs of large-scale industrial production.
By performing multi-site site-directed mutagenesis on phenylpyruvate decarboxylase derived from Saccharomyces cerevisiae, a highly efficient phenylpyruvate decarboxylase mutant was constructed and overexpressed in Escherichia coli. Combined with genetically engineered strains containing alcohol dehydrogenase, 3-deoxy-D-arabinoheptulose-7-phosphate synthase, cyclohexadiene dehydrogenase, and L-amino acid ammonia-lyase, de novo synthesis of tyrosol was achieved.
It significantly increased tyrosol production, with shake-flask fermentation levels reaching 3.6 g/L, providing essential building blocks for high-yield tyrosol strains and supporting industrial applications.