RNA preparations containing purified modified RNA for reprogramming cells
Purified single-stranded mRNA molecules with modified nucleosides efficiently reprogram eukaryotic cells to generate iPS cells, addressing inefficiencies and genomic risks of viral methods, ensuring rapid and safe production.
JP7763277B2Active Publication Date: 2025-10-31THE TRUSTEES OF THE UNIV OF PENNSYLVANIA
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Patent Information
- Application Number
- JP2024003556
- Authority / Receiving Office
- JP · JP
- Patent Type
- Patents
- Current Assignee / Owner
- Priority Date
- 2009-12-07
- Filing Date
- 2024-01-12
- Publication Date
- 2025-10-31
- Estimated Expiration
- 2030-12-07
AI Technical Summary
Technical Problem
Current methods for generating induced pluripotent stem cells (iPS cells) using viral delivery of reprogramming factors are inefficient and can lead to genomic integration, resulting in unpredictable outcomes and potential cancer risks.
Method used
Using purified single-stranded mRNA molecules containing modified nucleosides such as pseudouridine and 5-methylcytidine, free of RNA contaminants, to reprogram eukaryotic cells, including human cells, without genomic integration.
Benefits of technology
Achieves highly efficient and rapid generation of iPS cells with maintained genomic integrity, reducing the risk of cancer and immune responses.
✦ Generated by Eureka AI based on patent content.
Abstract
To provide compositions and methods for reprogramming somatic cells using purified RNA preparations comprising single-strand mRNA encoding an iPS cell inducing factor.SOLUTION: Provided is a method for inducing mammalian cells existing in vitro to produce a recombinant protein, the method including contacting the mammalian cells with a preparation of mRNA synthesized in vitro, containing an open reading frame encoding a recombinant protein, the mRNA being obtained by in vitro transcription and containing Ψ(5-β-D-ribofuranosyluracil), or Ψ(5-β-D-ribofuranosyluracil) containing a modified nucleobase, the mRNA preparation being purified using a purification step of removing RNA contaminant molecules that are immunogenic and toxic to the cells by inducing an innate immune response.SELECTED DRAWING: Figure 1
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