A molecular marker for identifying beef components and its application

By designing specific amplification primers to perform fluorescence quantitative PCR amplification of bovine genomic DNA, the problem of difficulty in accurately identifying and quantitatively detecting beef ingredients in foods in the prior art is solved, and high sensitivity and specific beef detection is achieved.

CN113789390BActive Publication Date: 2025-03-25SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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Patent Information

Application Number
CN202110659446.5
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2021-06-11
Publication Date
2025-03-25
Estimated Expiration
2041-06-11

AI Technical Summary

Technical Problem

It is difficult to accurately identify and quantitatively detect beef ingredients in foods, especially in foods that have been processed, frozen, and refrigerated.

Method used

A nucleic acid-based detection method was designed to achieve accurate identification and quantitative detection of beef ingredients by fluorescence quantitative PCR amplification of bovine genomic DNA using specific amplification primers CHR11F326 and CHR11R411.

Benefits of technology

This method can detect beef ingredients with high sensitivity and specificity, avoiding the limitations of protein detection methods, and is suitable for the detection of various meat products, including mixed meat products.

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Abstract

The present invention discloses a molecular marker for identifying beef ingredients and its application. Through the real-time fluorescence quantitative PCR technology based on SYBR Green I dye, a single copy gene of cattle in the cell nucleus is used as a molecular marker to establish a method for detecting beef ingredients with strong specificity and high sensitivity. The method established by the present invention can accurately distinguish cattle from 12 other animals and 6 plants, and can accurately determine whether 9 meat products on the market contain cattle-derived ingredients by actual testing. The cattle-specific real-time fluorescence quantitative PCR detection method established by the present invention can provide a technical reference for the qualitative and quantitative detection of cattle-derived ingredients in mixed meat products.
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