A MALDI-MS analysis method for small molecule compounds labeled with polycyclic aromatic hydrocarbon derivatives

By selectively labeling small-molecular compounds with polycyclic aromatic hydrocarbon derivatives, the problem of matrix interference and low sensitivity in small-molecular compound analysis by MALDI-MS is solved, and quantitative analysis and mass spectrometry imaging analysis with high sensitivity are achieved, which expands the scope of analysis application.

CN114636752BActive Publication Date: 2025-03-18ZHENGZHOU UNIV
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Patent Information

Application Number
CN202210265414.1
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-03-17
Publication Date
2025-03-18
Estimated Expiration
2042-03-17

AI Technical Summary

Technical Problem

MALDI-MS has problems of severe matrix interference and low detection sensitivity in small molecule compound analysis, which limits its application range.

Method used

Using polycyclic aromatic hydrocarbon derivatives as labeling reagents, MALDI-MS analysis was performed by selectively labeling small molecule compounds, avoiding the use of additional substrates and improving the detection sensitivity and signal strength of the analytes.

Benefits of technology

It effectively solved the problem of severe matrix interference and low sensitivity in small molecule compound analysis, expanded the application range of MALDI-MS in small molecule compound detection, and realized quantitative analysis and mass spectrometry imaging analysis.

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Abstract

The present invention relates to a MALDI-MS analysis method for small molecule compounds labeled with polycyclic aromatic hydrocarbon derivatives, which can effectively solve the problems of severe interference and low sensitivity existing in the current MALDI-MS analysis of small molecule compounds. The technical solution is to select a polycyclic aromatic hydrocarbon derivative consisting of a polycyclic aromatic hydrocarbon skeleton and a reactive group as a labeling reagent according to the groups contained in the target small molecule compound, so as to selectively label the small molecule compound, and the desorption / ionization efficiency of the labeled analyte is greatly improved, thereby improving the detection sensitivity; the labeled analyte can be detected without an additional matrix, thereby solving the matrix interference problem existing in the MALDI-MS analysis of small molecule compounds, thereby greatly promoting the application scope of MALDI-MS in the analysis of small molecule compounds, and is an innovation in the MALDI-MS analysis method of small molecule compounds.
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