High-efficiency fluorescence quenching test strip based on Fe-PDAN and preparation method and detection method thereof
By using iron ion-coordinated polydopamine (Fe-PDAN) as a beacon carrier, combined with fluorescence quenching technology, the detection mode is changed, and the existing immunochromatography technology is solved, and the problem of low sensitivity and susceptibility to interference is achieved, achieving higher detection sensitivity and stability.
CN114720437BActive Publication Date: 2025-03-25INSTITUTE OF MICROBIOLOGY JIANGXI ACADEMY OF SCIENCES (JIANGXI INSTITUTE OF WATERSHED ECOLOGY)
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Patent Information
- Application Number
- CN202210196123.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2022-03-01
- Publication Date
- 2025-03-25
- Estimated Expiration
- 2042-03-01
AI Technical Summary
Technical Problem
The existing immunochromatography technology has low sensitivity and is susceptible to matrix interference, making it difficult to meet the needs of efficient detection.
Method used
Metal polyphenol network microspheres (Fe-PDAN) formed by iron ion-coordinated polydopamine are used as beacon carrier, combined with fluorescence quenching technology, and the traditional "Turn off" mode is changed to "Turn on" mode to improve detection sensitivity.
Benefits of technology
Higher detection sensitivity and stability are achieved, overcoming the problems of low sensitivity and susceptibility to interference in the prior art.
✦ Generated by Eureka AI based on patent content.
Abstract
The present invention discloses a Fe-PDAN-based efficient fluorescence quenching test strip, and a preparation method and a detection method thereof. The fluorescence quenching test strip uses Fe-PDAN as a beacon carrier. The sample pad, a glass fiber pad sprayed with a Fe-PDAN test object antibody complex, a nitrocellulose membrane sprayed with an artificial coupled antigen of the test object and quantum dot fluorescent microspheres as a detection line and sprayed with anti-mouse antibodies or anti-rabbit antibodies as a quality control line, and absorbent paper are overlapped and pasted on a bottom plate in sequence for assembly. During detection, quantitative detection and qualitative judgment are achieved by reading the fluorescence intensity data of the fluorescence quenching test strip, or observing the presence or absence of fluorescent bands on the detection line under ultraviolet light. The fluorescence quenching test strip prepared by the present invention uses Fe-PDAN as a beacon carrier for the first time. Compared with PDAN, it has a stronger ability to quench the fluorescence of quantum dot fluorescent microspheres and high stability. The prepared fluorescence quenching test strip has strong stability and high detection sensitivity.
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