A microring resonator for detecting IgG protein in urine and its preparation method and application
By modifying the nanobiological functional membrane on the surface of the resonant cavity of the microring resonator, and using anti-IgG antibodies to react specifically with IgG proteins, the sensitivity and repetition of the existing detection methods are solved, and rapid, simple, and radiation-free IgG protein detection is achieved.
CN115824980BActive Publication Date: 2025-03-28QIANGANRUO MEDICAL & HEALTH TECHNOLOGY CO LTD
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Patent Information
- Application Number
- CN202211494975.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2022-11-26
- Publication Date
- 2025-03-28
- Estimated Expiration
- 2042-11-26
AI Technical Summary
Technical Problem
The existing methods for detecting IgG protein in urine have problems such as high cost of enzyme labeling, insufficient sensitivity, poor repeatability, easy to cause missed tests and false positives.
Method used
The nanobiological functional membrane is modified on the surface of the resonant cavity of the microring resonator through covalent bonding, and the anti-IgG antibody on the nanobiological functional membrane reacts specifically with the IgG protein to achieve detection.
Benefits of technology
This method does not require enzyme labeling and directly measures IgG protein, which is easy to operate, fast and sensitive, avoids radiation hazards, and can detect changes in the concentration of IgG protein in real time.
✦ Generated by Eureka AI based on patent content.
Abstract
The present invention belongs to the field of analytical sensing technology, and mainly relates to a preparation method of a microring resonator for detecting IgG protein in urine. The microring resonator is prepared by a method combining a nano-biofunctional membrane with a microring resonator. First, reagents such as anti-immunoglobulin G antibodies, polyamidoamine, succinic anhydride, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, (3-aminopropyl) triethoxysilane, N-hydroxysuccinimide are selected, and a nano-biofunctional membrane with specific recognition performance for IgG is prepared on the resonant cavity surface of the microring resonator by covalent coupling, and then encapsulated with an optical fiber array to obtain a microring resonator with specific recognition function for IgG. The microring resonator of the present invention can directly detect IgG protein without labeling and without radioactive hazards, and is expected to play an important role in clinical medical testing, medical treatment and biochemical analysis.
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