A system and method for rapid detection of heart rate and response to light environment of chicken embryos

Abstract

The application discloses a kind of quick detection system and method of chicken embryo heart rate and response degree to light environment, comprising: box, light source, egg test fixing frame, temperature control device, light source controller, infrared emission and receiving device, computing module;Egg test fixing frame is used to carry the egg to be detected;Temperature control device is used to control the temperature in box and keep constant temperature;Light source is used to provide light source for egg;Light source controller is used to adjust light source to emit light source of different intensity, different wavelength;Infrared emission device is used to emit infrared light to the chicken embryo inside egg;Infrared receiving device is used to receive the infrared light reflected by chicken embryo and convert into electrical signal transmission to computing module;Computing module is used to receive respectively the electrical signal transmitted by infrared receiving device when egg is under different intensity, different wavelength light source and no light source condition and carry out data processing, obtain the heart rate value of chicken embryo in egg.The application can quickly detect and obtain the heart rate of chicken embryo and its response degree to light environment.

Description

Technical Field

[0001] This invention relates to the field of agricultural breeding technology optimization, specifically to a rapid detection system and method for chicken embryo heart rate and its response to light environment. Background Technology

[0002] The heart rate of chicken embryos during development and their responsiveness to the incubation environment have always been key concerns in the poultry industry. While the application of light environment in the incubation environment has been widely used during poultry rearing, a mature technological system for its application during the incubation period is still lacking.

[0003] Heart rate is a crucial indicator for assessing the viability and health of chicken embryos and their responsiveness to the environment. Existing detection systems and methods for chicken embryos during development can only detect whether the embryo within the egg has developed. For example, patent application US11218013 discloses a method and apparatus for identifying and diagnosing live eggs using heart rate and embryonic movement. This patent's disclosed technical solution can only identify and diagnose whether the embryo within the egg has completed development, but it cannot detect the embryo's responsiveness to the incubation environment. Furthermore, during embryo development, different breeds of chicken embryos exhibit varying sensitivities to different light environment parameters. Therefore, a detection system and method are needed to assess the viability and health of the developing embryo and to screen for light environment parameters that the embryo is sensitive to. Summary of the Invention

[0004] Objective of the Invention: To address the above-mentioned technical problems, this invention provides a rapid detection system for chicken embryo heart rate and its responsiveness to light environments, capable of quickly detecting and acquiring the heart rate values ​​of chicken embryos under different light environments during incubation. This invention also provides a rapid detection method for chicken embryo heart rate and its responsiveness to light environments, capable of acquiring the heart rate values ​​of chicken embryos under different light environments during incubation, analyzing and determining the responsiveness of chicken embryos to different light environments during incubation, thereby providing corresponding light environment parameters for optimizing incubation conditions.

[0005] Technical solution: To solve the above problems, this invention discloses a rapid detection system for chicken embryo heart rate and response to light environment, specifically including: a box, a light source, a hatching egg testing fixture, a temperature control device, a light source controller, an infrared emitting device, an infrared receiving device, and a calculation module;

[0006] The egg testing fixture is fixed inside the box and is used to hold the eggs to be tested;

[0007] The temperature control device is installed inside the chamber to control the temperature inside the chamber and keep the temperature inside the chamber constant.

[0008] The light source is fixed inside the box and is used to provide light for the hatching eggs;

[0009] The light source controller is mounted on the housing and is used to adjust the light source to emit light sources of different intensities and wavelengths.

[0010] The infrared emitting device and the infrared receiving device are both installed inside the box and connected to the computing module; the infrared emitting device is used to emit infrared light into the chicken embryo inside the hatching egg; the infrared receiving device is used to receive the infrared light reflected by the chicken embryo and convert the infrared light into an electrical signal to be transmitted to the computing module.

[0011] The calculation module is used to receive and process the electrical signals transmitted by the infrared receiving device when the hatching egg is under light sources of different intensities and wavelengths, as well as under no light source conditions, and to identify and obtain the heart rate value of the chicken embryo in the hatching egg under light sources of different intensities and wavelengths, as well as under no light source conditions.

[0012] Furthermore, the hatching eggs to be tested are selected from those at different incubation cycles and with different shell colors; specifically, they are divided into light-colored, medium-colored, and dark-colored hatching eggs according to the depth of their shell color. By setting hatching eggs with different shell colors and different incubation cycles, the response of chicken embryos in different breeds of hatching eggs to different light environment parameters during incubation is detected.

[0013] Furthermore, it also includes a platform-shaped rubber ring; the platform-shaped rubber ring is located directly below the hatching egg to support the bottom of the egg; an infrared emitting device is attached to the platform-shaped rubber ring and in contact with the hatching egg, the infrared emitting device has several emitting tubes evenly distributed around the outer periphery of the hatching egg; an infrared receiving device is installed directly below the hatching egg testing fixture. The light signal received by the infrared receiving device changes with the chicken embryo's heart rate. By setting three emitting tubes and tightly attaching the platform-shaped rubber ring to the hatching egg, and placing the infrared receiving device directly below the hatching egg testing fixture, the signal changes can be obtained more accurately, facilitating subsequent heart rate identification.

[0014] Furthermore, the light source is fixed to the top of the inner wall of the box and located directly above the hatching eggs; the light source is an LED spotlight, and the light emitted by the LED spotlight is concentrated on the eggshell of the hatching eggs.

[0015] Furthermore, the calculation module performs data processing on the received signal, specifically including filtering and amplification. To accurately obtain the heart rate of the chicken embryo within a specific time period, the acquired signal is filtered and amplified.

[0016] Furthermore, a temperature sensor is installed inside the chamber; this sensor is connected to a computing module to acquire the temperature inside the chamber in real time, transmit the data to the computing module, and display it on the screen. This facilitates real-time monitoring of the temperature inside the chamber, ensuring that all eggs to be tested are at the same suitable incubation temperature.

[0017] Furthermore, the box is equipped with a sponge pad, on which a platform-shaped rubber ring is placed; the infrared light emitted by the infrared transmitter and the reflected light received by the infrared receiver can both pass through the sponge pad for transmission, and the sponge pad serves a protective function.

[0018] Furthermore, the enclosure is made of LLDPE material, and the exterior is covered with a light-blocking cloth. LLDPE is an excellent insulation material, which helps to keep the enclosure warm. The light-blocking cloth further prevents the influence of external light.

[0019] Furthermore, this invention discloses a rapid detection method for chicken embryo heart rate and its response to light environment, comprising the following steps:

[0020] (1) Adjust the temperature control device to keep the temperature inside the box at a suitable incubation temperature for the eggs;

[0021] (2) Place the hatching eggs to be tested on the hatching egg test rack and let them stand for 5 minutes;

[0022] (3) When the hatching egg is not exposed to light, the infrared emitting device is controlled to emit infrared light, the infrared receiving device receives the infrared light reflected by the hatching egg to be tested and converts the infrared light into an electrical signal, and transmits the electrical signal to the calculation module to obtain the heart rate value of the chicken embryo.

[0023] (4) Turn on the light source and adjust the light source controller to make the light source emit light sources of different intensities and wavelengths, so that the hatching eggs are exposed to light sources of different intensities and wavelengths for a certain period of time.

[0024] (5) When the hatching eggs are exposed to light sources of different intensities or wavelengths, the infrared emitting device is controlled to emit infrared light, the infrared receiving device receives the infrared light reflected by the hatching eggs to be tested and converts the infrared light into an electrical signal, and transmits the electrical signal to the calculation module; the calculation module obtains the heart rate value of the chicken embryo under light sources of different intensities and wavelengths respectively.

[0025] (6) Select a hatching egg that is in a different incubation period than the current hatching egg to be tested and replace it as a new hatching egg to be tested. Repeat steps (3) to (5).

[0026] (7) Compare and analyze the heart rate values ​​of chicken embryos in the same incubation period under no light source conditions, under light source conditions of different intensities, and under light source conditions of different wavelengths, and determine the heart rate response of chicken embryos in the same incubation period to different light environment parameters.

[0027] Furthermore, it also includes:

[0028] (8) Select a hatching egg that is in the same incubation cycle as the current hatching egg to be tested but has a different shell color and replace it as a new hatching egg to be tested. Repeat steps (3) to (5).

[0029] (9) Compare and analyze the heart rate values ​​of chicken embryos under specific light intensity conditions and under specific wavelength light conditions for hatching eggs with different shell colors in the same incubation period; determine the differences in heart rate response of chicken embryos in hatching eggs with different shell colors in the same incubation period to specific light environment parameters.

[0030] Beneficial Effects: This invention provides a rapid detection system for chicken embryo heart rate and its response to light environment. Compared with existing technologies, its significant advantages are: 1. By setting up an infrared emitting and receiving device, combined with a calculation module, the heart rate of the chicken embryo is identified and obtained; and by adjusting the intensity and wavelength of the light source at the top of the chamber to change the light environment parameters of the chicken embryo, the heart rate values ​​of the chicken embryo in the hatching egg under different light environment parameters are obtained through multiple detections, thereby determining the heart rate response of the chicken embryo to different light environment parameters; 2. By setting up several hatching eggs with different incubation cycles and different eggshell colors to be tested, the heart rate response of chicken embryos in a specific incubation cycle to different light environment parameters and the differences in the heart rate response of chicken embryos with different eggshell colors to different light environment parameters are further determined. This invention provides a rapid detection method for chicken embryo heart rate and its response to light environment. Compared with existing technologies, its significant advantages are the same as the system advantages: it can obtain the heart rate value of the chicken embryo and the heart rate response of the chicken embryo to different light environment parameters, providing a basis for chicken embryo incubation light environment parameters, improving incubation effect, and reducing the cost of light environment incubation operations. Attached Figure Description

[0031] Figure 1 The diagram shown is a structural schematic of the system described in this invention;

[0032] Figure 2 The diagram shows a flowchart of data processing performed by the computing module in the system described in this invention.

[0033] Figure 3 The diagram shown is a circuit diagram of the infrared sensor device in the system described in this invention.

[0034] Figure 4 The diagram shows a flowchart of the method described in this invention. Detailed Implementation

[0035] The technical solution of the present invention will be further described below with reference to the accompanying drawings.

[0036] like Figure 1As shown, the present invention provides a rapid detection system for chicken embryo heart rate and its response to light environment, specifically comprising: a calculation module 1, a housing 5, a temperature sensor 6, a hatching egg testing bracket 7, a sponge pad 8, an infrared receiver 9, a light source controller 10, a power supply 11, a light source bracket 12, a light source 13, hatching eggs 14, an infrared emitting device 15, a platform-shaped rubber ring 16, and a temperature control device 17. The calculation module 1 includes wires 2, a microcontroller 3, and a display screen 4. The display screen 4 is connected to the microcontroller 3 via a USB-to-serial port for power supply.

[0037] The housing 5 includes a lid 18, and the housing 5 is made of LLDPE. An egg testing rack 7 is installed inside the housing 5 to hold the eggs 14 to be tested. The eggs 14 to be tested are selected from eggs in different incubation cycles and with different shell colors. In this embodiment, Wenshi Meihua No. 5 eggs are selected. These are pink eggs. A colorimeter is used to measure the color of the eggshell surface, dividing it into three color ranges: light-colored eggs (19.25-30.39), medium-colored eggs (30.47-34.94), and dark-colored eggs (34.99-44.09). The stable heart rate range of the chicken embryo inside the egg varies depending on the incubation cycle. Specifically, the incubation cycle is 21 days: days 1-7 are the early stage of embryonic development, days 8-14 are the middle stage, and days 15-21 are the late stage. In this embodiment, 9-day-old chicken embryos and 15-day-old chicken embryos were used.

[0038] A temperature control device 17 is installed inside the housing 5 and located at the bottom of the housing 5. The temperature control device 17 is connected to the display screen 4 via wires, and specifically uses a PET heating film combined with a reflective film. The temperature control device 17 is used to control the temperature inside the housing 5 and maintain a constant temperature inside the housing 5. A temperature sensor 6 is installed inside the housing 5 and connected to the microcontroller 3 via wires. The temperature sensor 6 is used to monitor the temperature inside the housing 5 in real time and feed it back to the display screen 4. In this embodiment, the microcontroller 3 is model STC89C52RC. The suitable incubation temperature for the Wenshi Meihua No. 5 hatching eggs selected in this embodiment is 37.8℃. Temperatures higher or lower than this will cause a sudden drop or rise in the embryo's heartbeat. Therefore, in this embodiment, it is necessary to ensure that the ambient temperature inside the housing 5 is constant at 37.8℃.

[0039] The light source 13 is fixed to the bottom of the box cover 18 by the light source mounting bracket 12 and is located directly above the hatching eggs 14; the light source 13 is an LED spotlight, and the light emitted by the LED spotlight is concentrated on the eggshell of the hatching eggs 14. The light source controller 10 is installed on the box and is used to adjust the light source 13 to emit light sources of different intensities and wavelengths; the power supply 11 is used to provide power to the light source 13.

[0040] The platform-shaped rubber ring 3 is located below the hatching egg 14 and placed above the sponge to support the bottom of the hatching egg 14; the infrared emitting device 15 is attached to the inner ring of the platform-shaped rubber ring 3 and is in contact with the hatching egg 14; the infrared emitting device 15 has three emitting tubes and is evenly distributed around the outer periphery of the hatching egg 14; the infrared receiving device 9 is installed directly below the hatching egg test fixture 7.

[0041] Infrared transmitter 15 and infrared receiver 9 are both installed inside the housing and connected to computing module 1. Infrared transmitter 15 emits infrared light into the chicken embryo inside hatching egg 14. Infrared receiver 9 receives the infrared light reflected by the chicken embryo, converts it into an electrical signal, and transmits it to computing module 1. The infrared light emitted by infrared transmitter 15 and the reflected light received by infrared receiver 9 can both pass through the platform-shaped rubber ring 16 and the sponge pad 8 for transmission.

[0042] Specifically, in this embodiment, the infrared emitting device 15 and the infrared receiving device 9 are respectively selected from the transmitting and receiving tubes of the ST188 photoelectric sensor. The working circuit of the ST188 photoelectric sensor is as follows: Figure 3 As shown, when the receiving tube receives reflected infrared light, the infrared receiving head is turned on, and the E pin outputs a high level, close to VCC; if no reflected infrared light is received, the infrared receiving head is not turned on, and the E pin outputs a low level, close to GND. The state of the ST188 photoelectric sensor's receiving tube can be determined by scanning the E pin with a microcontroller.

[0043] The calculation module 1 is used to receive and process the electrical signals transmitted by the infrared receiving device under different intensities and wavelengths of light sources and under no light source conditions, respectively; and finally identify and obtain the heart rate values ​​of the chicken embryos in the hatching eggs 14 under different intensities and wavelengths of light sources and under no light source conditions. Figure 2 As shown, the calculation module 1 performs data processing on the received signal, specifically including RC filtering, signal amplification, and shaping.

[0044] like Figure 4 As shown, this invention discloses a rapid detection method for chicken embryo heart rate and its response to light environment, comprising the following steps:

[0045] Step 1: Monitor the current temperature inside the chamber in real time using a temperature sensor, and adjust the temperature control device to keep the temperature inside the chamber at the suitable incubation temperature for the eggs, i.e., 37.8℃.

[0046] Step 2: Place the hatching eggs to be tested on the hatching egg testing rack and let them stand for 5 minutes. A light-blocking cloth is placed on the outer surface of the box. In this embodiment, the hatching eggs to be tested are Wenshi Meihua No. 5 hatching eggs.

[0047] Step 3: When the hatching egg is not exposed to light, control the infrared emitting device to emit infrared light, and the infrared receiving device to receive the infrared light reflected by the hatching egg to be tested and convert the infrared light into an electrical signal. The electrical signal is transmitted to the calculation module, and the heart rate value of the chicken embryo is obtained through the calculation module.

[0048] Since the hatching eggs selected in this embodiment generally achieve a stable heart rate value after 8 days of age, and the heart rate range of healthy chicken embryos after 8 days of age is around 200-300 beats per minute. If the heart rate value of the chicken embryo is detected to be lower than 200 under a constant and suitable temperature environment, the chicken embryo developing in this hatching egg is a weak chick. If the heart rate value is detected to be close to 0, the hatching egg is an infertile egg or a dead embryo.

[0049] Step 4: Select hatching eggs with chicken embryo heart rate within the normal range; turn on the light source and adjust the light source controller to emit light sources of different intensities and wavelengths, ensuring that the hatching eggs are exposed to light sources of different intensities and wavelengths for a certain period of time.

[0050] In this embodiment, the different light intensities specifically include 300 lx, 150 lx, and 50 lx, and the different wavelengths of light sources specifically include monochromatic red light, monochromatic green light, and monochromatic blue light. The specified time is generally 3 minutes, that is, the hatching eggs are exposed to a light source of a specific intensity and wavelength for 3 minutes before the subsequent heart rate reading operation is performed.

[0051] Step 5: When the hatching eggs are exposed to light sources of different intensities or wavelengths, the infrared emitting device is controlled to emit infrared light, and the infrared receiving device receives the infrared light reflected by the hatching eggs to be tested and converts the infrared light into an electrical signal, which is then transmitted to the calculation module. The calculation module then obtains the heart rate value of the chicken embryo under light sources of different intensities and wavelengths.

[0052] Step 6: Select a hatching egg that is in the same incubation cycle as the current hatching egg to be tested but has a different shell color and replace it as the new hatching egg to be tested. Repeat steps 3 to 5.

[0053] Step 7: Select a hatching egg that is in a different incubation period than the current hatching egg to be tested and replace it as the new hatching egg to be tested. Repeat steps 3 to 6.

[0054] Step 8: Compare and analyze the heart rate values ​​of chicken embryos in hatching eggs of the same incubation period but different shell colors under specific light intensity conditions and under specific wavelength light conditions; determine the differences in heart rate response of chicken embryos in hatching eggs of the same incubation period but different shell colors to specific light environment parameters. Also, compare and analyze the heart rate values ​​of chicken embryos in hatching eggs of the same incubation period under no light source conditions, under light source conditions of different intensities, and under light source conditions of different wavelengths; determine the heart rate response of chicken embryos in hatching eggs of the same incubation period to different light environment parameters.

[0055] The results of the egg response test using the method described in this invention are shown in Tables 1 to 6 below. Table 1 shows the heart rate response test results of 9-day-old chicken embryos to different monochromatic green light intensities.

[0056] Table 1

[0057]

[0058] Table 1 shows the heart rate response of hatching eggs to different monochromatic green light intensities at 9 days of incubation. Specifically, at intensities of 300 lx and 150 lx, the heart rate values ​​of chicken embryos in light, medium, and dark colored eggs significantly increased after the change in light environment (before the test, the eggs were in a state of no light; after the test, the eggs were exposed to monochromatic green light of a specific intensity) (P < 0.05), and there was no significant difference in the heart rate change values ​​among the three colored eggs (p > 0.05). At an intensity of 50 lx, the heart rate response value of the hatching eggs was slightly lower than that at 300 lx and 150 lx, and the heart rate values ​​of the three colored eggs also significantly increased (P < 0.05). However, there was a significant difference in the heart rate change values ​​among the three colored eggs (P < 0.05). There was no significant difference between light and medium colored eggs (P > 0.05), but the heart rate values ​​of both groups of eggs were significantly higher than those of dark colored eggs.

[0059] Therefore, it can be seen that 9-day-old chicken embryos show a significant response to monochromatic green light with an intensity of 50 lx or higher. However, at 50 lx, the heart rate response of chicken embryos in darker colored eggs is slightly reduced, which is related to the fact that the depth of the eggshell surface color can reduce light transmittance. If monochromatic green light is used to stimulate chicken embryos during this growth period, a light intensity of 50 lx is sufficient to ensure the responsiveness of the chicken embryos.

[0060] Table 2 shows the heart rate response test results of 9-day-old chicken embryos to different monochromatic red light intensities.

[0061] Table 2

[0062]

[0063] Table 2 shows the heart rate response of hatching eggs to different monochromatic red light intensities at 9 days of incubation. Specifically, at intensities of 300 lx, 150 lx, and 50 lx, the heart rate values ​​of chicken embryos in light, medium, and dark colored eggs all increased significantly after the change in light environment (before the test, the eggs were in a dark state; after the test, the eggs were exposed to monochromatic red light of a specific intensity) (P < 0.05), and there was no significant difference in the heart rate change values ​​among the three colored eggs (P > 0.05). Under light environments of 50 lx and 150 lx, the heart rate change values ​​of light-colored eggs were slightly higher than those of medium and dark-colored eggs, but the difference was not significant (P > 0.05).

[0064] Therefore, it can be seen that 9-day-old chicken embryos also showed significant responses to monochromatic red light environments of 50 lx and above (P < 0.05). If monochromatic red light is used to stimulate chicken embryos during this growth period, a light intensity of 50 lx is sufficient to ensure the responsiveness of the chicken embryos.

[0065] Table 3 shows the heart rate response test results of 9-day-old chicken embryos to different monochromatic blue light intensities.

[0066] Table 3

[0067]

[0068] Table 3 shows the heart rate response of hatching eggs to different monochromatic blue light intensities at day 9 of incubation. Specifically, at 300 lx, the heart rate of hatching eggs of light, medium, and dark colors all increased significantly after the change in light environment (before the test, the eggs were in a dark state; after the test, the eggs were exposed to monochromatic blue light of a specific intensity) (P < 0.01), and there was no significant difference in the heart rate change values ​​among the three color types of eggs (P > 0.05). At 150 lx, only the heart rate values ​​of chicken embryos in light and medium color eggs showed a significant increase (P < 0.05). At 50 lx, the heart rate values ​​of chicken embryos in light, medium, and dark color eggs did not change significantly before and after the change in light environment (P > 0.05), and there was no significant difference in the heart rate change values ​​among the three color types of eggs (P > 0.05).

[0069] This indicates that 9-day-old chicken embryos show a significant response to monochromatic blue light environments of 300 lx and above. If monochromatic blue light is used to stimulate chicken embryos during this growth period, a light intensity of 300 lx is required to ensure the embryos' responsiveness.

[0070] Table 4 shows the heart rate response test results of 15-day-old chicken embryos to different monochromatic green light intensities.

[0071] Table 4

[0072]

[0073]

[0074] Table 4 shows the heart rate response of hatching eggs to different monochromatic green light intensities at 15 days of incubation. Specifically, at intensities of 300 lx and 150 lx, the heart rate values ​​of chicken embryos in light, medium, and dark colored eggs all increased significantly after the change in light environment (before the test represents the state without light, and after the test represents the state under monochromatic blue light irradiation of a specific intensity) (P < 0.05), and there was no significant difference in the heart rate change values ​​of the three colored eggs (P > 0.05).

[0075] Therefore, it can be concluded that 15-day-old chicken embryos have a significant response to monochromatic green light environments of 150 lx and above (P < 0.05). Furthermore, comparing Table 4 with Table 1 shows that at 9 days of age, because the eyelids of the chicken embryos do not cover the eyeballs and the embryos are relatively small, light can be scattered to the embryos through substances such as egg white and albumen, thus the embryonic heart rate responds to 50 lx light intensity. However, at 15 days of age, the chicken embryos develop rapidly, occupying approximately 2 / 3 of the egg's volume, and the eyelids cover the embryonic eyeballs; therefore, 50 lx light intensity does not produce a significant response in the embryonic heart rate.

[0076] Table 5 shows the heart rate response test results of 15-day-old chicken embryos to different monochromatic red light intensities.

[0077] Table 5

[0078]

[0079] Table 5 shows the heart rate response of hatching eggs to different monochromatic red light intensities after 15 days of incubation. Specifically, at intensities of 300 lx, 150 lx, and 50 lx, the heart rate values ​​of chicken embryos in light, medium, and dark colored eggs all increased significantly after changes in the light environment (before the test represents a state without light, and after the test represents a state under monochromatic red light irradiation of a specific intensity) (P < 0.05). There was no significant difference in the heart rate change values ​​among the three colored eggs (P > 0.05), but the heart rate change values ​​of chicken embryos in light-colored eggs were slightly higher than those in medium and dark-colored eggs.

[0080] Therefore, it can be seen that 15-day-old chicken embryos also showed significant responses to monochromatic red light environments of 50 lx and above (P < 0.05). If monochromatic red light is used to stimulate chicken embryos during this growth period, a value of 50 lx or above is required to ensure the responsiveness of the chicken embryos.

[0081] Table 6 shows the heart rate response test results of 15-day-old chicken embryos to different monochromatic blue light intensities.

[0082] Table 6

[0083]

[0084] Table 6 shows the heart rate response of hatching eggs to different monochromatic blue light intensities after 15 days of incubation. Specifically, at 300 lx intensity, the heart rate of light, medium, and dark colored eggs all increased significantly after changes in the light environment (before the test represents no light, and after the test represents being under monochromatic blue light of a specific intensity) (P < 0.05), and there was no significant difference in the heart rate changes among the three colored eggs (P > 0.05). At 150 lx intensity, the heart rate of the embryos in light and medium colored eggs increased significantly after changes in the light environment (P < 0.05), but the heart rate of the embryos in dark colored eggs did not change significantly after changes in the light environment (P > 0.05). At 50 lx intensity, the heart rate of the embryos in light, medium, and dark colored eggs did not change significantly after changes in the light environment (P > 0.05).

[0085] Therefore, it can be concluded that 15-day-old chicken embryos have a significant response to monochromatic blue light environments of 300 lx and above (P < 0.05). At 150 lx, only the heart rate values ​​of chicken embryos in light and medium color eggs showed a significant increase (P < 0.05). If monochromatic blue light is used to stimulate chicken embryos during this growth period, 300 lx is required to ensure the responsiveness of the embryos.

[0086] Analysis of Tables 1 to 6 shows that under monochromatic green light, only 50 lx is needed to produce a significant response in the early to mid-stages of chicken embryo development (8-14 days old), while 150 lx or higher is required in the later stages (15-21 days old). Under monochromatic red light, only 50 lx is needed to produce a significant response throughout chicken embryo development, but in the mid to late stages, the change in heart rate response at 50 lx is slightly lower than at 150 lx and 300 lx. Furthermore, the development speed is faster in the later stages of embryonic development. As the embryo develops, the eyelids cover the eyeball, and the probability of light passing through the eggshell to reach the photoreceptors decreases. Therefore, higher light intensities may be needed to produce a response in the later stages of development. Under monochromatic blue light, 150 lx is needed to produce a significant response in the early to mid-stages of chicken embryo development (9 days old), while 300 lx is needed in the mid to late stages (15 days old).

[0087] In summary, by using the method described in this invention, it is possible to quickly screen out hatching eggs with a heart rate within the normal range and confirm the responsiveness of chicken embryos in hatching eggs with different incubation cycles and different shell colors to the light environment, thereby providing the corresponding light environment and optimizing incubation conditions.

Claims

1. A system for rapid detection of heart rate and responsiveness to light environment of chicken embryos, characterized in that, include: The components include: housing, light source, egg testing rack, temperature control device, light source controller, infrared transmitter, infrared receiver, and computing module. The egg testing fixture is fixed inside the box and is used to hold the eggs to be tested; The temperature control device is installed inside the chamber to control the temperature inside the chamber and keep the temperature inside the chamber constant. The light source is fixed inside the box and is used to provide light for the hatching eggs; The light source controller is mounted on the housing and is used to adjust the light source to emit light sources of different intensities and wavelengths. The infrared emitting device and the infrared receiving device are both installed inside the box and connected to the computing module; the infrared emitting device is used to emit infrared light into the chicken embryo inside the hatching egg; the infrared receiving device is used to receive the infrared light reflected by the chicken embryo and convert the infrared light into an electrical signal to be transmitted to the computing module. The calculation module is used to receive and process the electrical signals transmitted by the infrared receiving device when the hatching egg is under light sources of different intensities and wavelengths, as well as under no light source conditions, and to identify and obtain the heart rate value of the chicken embryo in the hatching egg under light sources of different intensities and wavelengths, as well as under no light source conditions.

2. The system for rapid detection of heart rate and responsiveness to light environment of chicken embryos according to claim 1, characterized in that, The hatching eggs to be tested were selected from hatching eggs in different incubation cycles and with different shell colors; based on the depth of the shell color, they were specifically divided into light-colored hatching eggs, medium-colored hatching eggs, and dark-colored hatching eggs.

3. The rapid detection system for chicken embryo heart rate and its response to light environment according to claim 1, characterized in that, It also includes platform-shaped rubber bands; The platform-shaped rubber ring is located directly below the hatching egg and is used to support the bottom of the hatching egg; the infrared emitting device is attached to the platform-shaped rubber ring and is in contact with the hatching egg, and the infrared emitting device has several emitting tubes that are evenly distributed around the outer periphery of the hatching egg; the infrared receiving device is installed directly below the hatching egg testing fixture.

4. The system for rapid detection of heart rate and responsiveness to light environment of chicken embryos according to claim 1, characterized in that, The light source is fixed to the top of the inner wall of the box and located directly above the hatching eggs; the light source is an LED spotlight, and the light emitted by the LED spotlight is concentrated on the eggshell.

5. The system for rapid detection of heart rate and responsiveness to light environment of chicken embryos according to claim 1, wherein, The calculation module performs data processing on the received signal, specifically including filtering and amplifying the received signal.

6. The system for rapid detection of heart rate and responsiveness to light environment of chicken embryos according to claim 1, wherein, A temperature sensor is also installed inside the enclosure; the temperature sensor is connected to the computing module to acquire the temperature inside the enclosure in real time and transmit it to the computing module for display.

7. The rapid detection system for chicken embryo heart rate and its response to light environment according to claim 3, characterized in that, The box is also equipped with a sponge pad, and a platform-shaped rubber ring is placed on the sponge pad; the infrared light emitted by the infrared transmitter and the reflected light received by the infrared receiver can both pass through the sponge pad for transmission.

8. The rapid detection system for chicken embryo heart rate and its response to light environment according to claim 1, characterized in that, The enclosure is made of LLDPE material and has a light-blocking cloth on its exterior.

9. A rapid detection method for chicken embryo heart rate and its response to light environment based on the system described in any one of claims 1 to 8, characterized in that, Includes the following steps: (1) Adjust the temperature control device to keep the temperature inside the box at a suitable incubation temperature for the eggs; (2) Place the hatching eggs to be tested on the hatching egg test rack and let them stand for 5 minutes; (3) When the hatching egg is not exposed to light, the infrared emitting device is controlled to emit infrared light, the infrared receiving device receives the infrared light reflected by the hatching egg to be tested and converts the infrared light into an electrical signal, and transmits the electrical signal to the calculation module to obtain the heart rate value of the chicken embryo. (4) Turn on the light source and adjust the light source controller to make the light source emit light sources of different intensities and wavelengths, so that the hatching eggs are exposed to light sources of different intensities and wavelengths for a certain period of time. (5) When the hatching eggs are exposed to light sources of different intensities or wavelengths, the infrared emitting device is controlled to emit infrared light, the infrared receiving device receives the infrared light reflected by the hatching eggs to be tested and converts the infrared light into an electrical signal, and transmits the electrical signal to the calculation module; the calculation module obtains the heart rate value of the chicken embryo under light sources of different intensities and wavelengths respectively. (6) Select a hatching egg that is in a different incubation period than the current hatching egg to be tested and replace it as a new hatching egg to be tested. Repeat steps (3) to (5). (7) Compare and analyze the heart rate values ​​of chicken embryos in the same incubation period under no light source conditions, under light source conditions of different intensities, and under light source conditions of different wavelengths, and determine the heart rate response of chicken embryos in the same incubation period to different light environment parameters.

10. The method of claim 9, wherein the method is performed on a chicken embryo. Also includes: (8) Select a hatching egg that is in the same incubation cycle as the current hatching egg to be tested but has a different shell color and replace it as a new hatching egg to be tested. Repeat steps (3) to (5). (9) Compare and analyze the heart rate values ​​of chicken embryos under specific light intensity conditions and under specific wavelength light conditions for hatching eggs with different shell colors in the same incubation period; determine the differences in heart rate response of chicken embryos in hatching eggs with different shell colors in the same incubation period to specific light environment parameters.

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