A nanobody against Clostridium perfringens alpha toxin and its application

By preparing nano-antibodies that specifically bind C. perfringens alpha toxin, the problem of detecting and binding C. perfringens alpha toxin in the prior art is solved, and high sensitivity detection and potential therapeutic effects are achieved.

CN115850466BActive Publication Date: 2025-08-05SHANXI AGRI UNIV
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Patent Information

Application Number
CN202211021233.0
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-08-24
Publication Date
2025-08-05
Estimated Expiration
2042-08-24

AI Technical Summary

Technical Problem

The prior art is difficult to detect and combine Clostridium perfringens alpha toxin quickly and sensitively, and lacks effective treatment methods.

Method used

A C. perfringens alpha toxin nanobody was developed. Through specific amino acid sequence design and screening methods, nanobody that can specifically bind to C. perfringens alpha toxin protein was prepared and applied to ELISA detection and treatment.

Benefits of technology

High sensitivity detection (0.625 μg/ml) and specific binding of C. perfringens alpha toxin was achieved, and 2 μg/ml of C. perfringens alpha toxin was detected in chicken and alpaca feces, providing rapid detection and potential treatment methods.

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Abstract

The present invention provides a Clostridium perfringens alpha-toxin nanobody and its application, belonging to the field of bioengineering technology. The amino acid sequence of the Clostridium perfringens alpha-toxin nanobody is shown in SEQ ID No. 1. The Clostridium perfringens alpha-toxin nanobody provided by the present invention can specifically bind to the Clostridium perfringens alpha-toxin protein with a minimum sensitivity of 0.625 μg / ml to the protein; and can detect Clostridium perfringens alpha-toxin in chicken and alpaca feces by ELISA with a sensitivity of 2 μg / ml.
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