A mutant PDS polypeptide and its application

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By directed evolution of the PDS gene, a resistant PDS polypeptide is obtained and the polypeptide is expressed in plants, which solves the tolerance of resistant weeds to PDS inhibitor herbicides, achieving efficient herbicidal effect and improving crop growth.

CN115851636BActive Publication Date: 2025-03-21SHANDONG SHUNFENG BIOTECH CO LTD

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Patent Information

Application Number: CN202211646798.8
Authority / Receiving Office: CN · China
Patent Type: Patents(China)
Current Assignee / Owner
Priority Date: 2021-12-24
Filing Date: 2022-12-21
Publication Date: 2025-03-21
Estimated Expiration: 2042-12-21

Application Information

Patent Timeline

24 Dec 2021

Priority

21 Dec 2022

Application

CN202211646798.8

21 Mar 2025

Publication

CN115851636B

IPC: C12N9/02; C12N15/53; C12N15/84; A01H5/00; A01H6/46

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Application Domain

Oxidoreductases Fermentation

Technical Efficacy Phrases

Increase resistance

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AI Technical Summary

Technical Problem

The prior art is difficult to effectively deal with resistant weeds, especially because weeds are resistant to PDS inhibitor-type herbicides, resulting in a reduced efficiency of herbicide use.

Method used

By directed evolution of the PDS gene, a mutant PDS polypeptide that is resistant or tolerant to PDS inhibitor-like herbicides is obtained and the polypeptide is expressed in plants to improve the tolerance of plants to these herbicides.

Benefits of technology

The plants have achieved high efficiency tolerance to PDS inhibitor herbicides, significantly improved the resistance of plants to these herbicides, reduced the amount of herbicides used, and improved the growth performance of crops.

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Abstract

The present invention provides a herbicide-resistant gene, a polypeptide and an application thereof in plant breeding. Specifically, the present invention provides a mutant PDS polypeptide, wherein the mutant PDS polypeptide is mutated at the amino acid position 538 corresponding to SEQ ID No. 1 compared with the parent PDS polypeptide. The mutated PDS polypeptide has a strong tolerance to herbicides and has a very broad application prospect in the field of improving and cultivating plants resistant to PDS inhibitor herbicides.

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