A KASP molecular marker for identifying orange and its application

By developing KASP molecular markers based on two consecutively replaced SNP sites in the Zongcheng CsSGRa sequence, the accurate identification of Zongcheng is achieved, the problem of difficulty in identifying Zongcheng and similar varieties is solved, and technical support for anti-counterfeiting and variety rights protection is provided.

CN115976257BActive Publication Date: 2025-03-25HUAZHONG AGRI UNIV
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Patent Information

Application Number
CN202211286715.9
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-10-20
Publication Date
2025-03-25
Estimated Expiration
2042-10-20

AI Technical Summary

Technical Problem

The existing technology is difficult to effectively distinguish between Zong orange and other similar varieties, resulting in frequent consumer rights disputes. After the scale of Zong orange planting expands, the demand for virus-free seedlings increases. How to identify the authenticity of Zong orange seedlings during the seedling stage has become a problem.

Method used

A KASP molecular marker based on two consecutively replaced SNP sites in the CsSGRa sequence of the lignin CsSGRa was developed, and the nucleotide type was detected by PCR to achieve accurate identification of the lignin.

Benefits of technology

The KASP molecular marker can clearly distinguish between the clone orange and other citrus varieties, solve the problem of difficulty in identifying clone orange and similar varieties, and provides technical support for the anti-counterfeiting of clone orange fruits and seedlings and the protection of variety rights.

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Abstract

The present invention discloses a KASP molecular marker for identifying zong orange and its application. The development of the marker is based on two consecutive SNP sites in the coding region of the citrus stay-green gene CsSGRa. The nucleotide type of the site in zong orange is TA, and the nucleotide type of the site in the control variety is GT. Using the forward and reverse primers and technical solutions for amplifying the site disclosed in the present invention, we can clearly distinguish zong orange from Australian chocolate orange and new zong orange, which are also sweet oranges and have a stay-green phenotype, and can also clearly distinguish it from other 78 citrus varieties tested. The molecular marker provided by the present invention can simply, accurately and efficiently identify zong orange and its similar varieties, provide effective technical support for the anti-counterfeiting, identification and variety rights protection of zong orange fruits and seedlings, and have important significance for the standardization and stable development of the zong orange industry.
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