Application of a preparation for inhibiting the expression of porcine RAB32 gene in the preparation of a drug for preventing porcine pseudorabies virus infection

The CRISPR/Cas9 knockout technology inhibited the expression of pig RAB32 gene, and a cell model against pseudorans infection was developed, which solved the problem of difficulty in effectively targeting PRV infection in the existing technology, achieved the inhibition of PRV proliferation and the development of new vaccine drugs, and provided new candidate target genes for anti-PR breeding research.

CN116271040BActive Publication Date: 2025-03-28HUAZHONG AGRI UNIV
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Patent Information

Application Number
CN202310051315.8
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-02-02
Publication Date
2025-03-28
Estimated Expiration
2043-02-02

AI Technical Summary

Technical Problem

The prior art is difficult to effectively target pseudorabies virus (PRV) infection, and the lack of target genes that can be used for disease-resistant breeding, which seriously restricts the development of drug targeting PRV and the progress of disease-resistant breeding.

Method used

Through CRISPR/Cas9 knockout technology, a cell model of anti-PRV infection was developed to target the expression preparation of the RAB32 gene knockout.

Benefits of technology

The inhibition of PRV proliferation has been achieved, new targets are provided for the development of new vaccines and drugs that effectively prevent and treat PR safely, and new candidate target genes are provided for anti-PR breeding research.

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Abstract

The present invention belongs to the field of genetic engineering and biomedicine, and discloses the use of a preparation for inhibiting the expression of the porcine RAB32 gene in the preparation of a porcine anti-pseudorabies virus infection drug, wherein the NCBI accession number of the mRNA of the RAB32 gene in the pig is NM_001123176.1. The present invention finds that knocking out the gene in PK15 cells can inhibit the proliferation of PRV, so the gene can be used as a new target for the development of vaccine drugs that inhibit the release of PRV and for research on disease-resistant breeding.
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