A growth promoter for the generation and swelling of sweet potato-shaped roots of Bougainvillea spectabilis and its application

Abstract

The present invention relates to the technical field of plant growth regulators, and more particularly to a growth promoter for the formation and expansion of tuber roots of bougainvillea and its use. The growth promoter comprises a tuber root generator and a tuber root expansion agent; the tuber root generator comprises phosphate, magnesium sulfate, Bacillus subtilis powder, aminochitosan oligosaccharide, seaweed fish protein liquid, uniconazole, and brassinolide; and the tuber root expansion agent comprises phosphate, magnesium sulfate, Bacillus subtilis powder, aminochitosan oligosaccharide, uniconazole, and brassinolide. By adding Bacillus subtilis and seaweed fish protein liquid to the formula and adjusting the type of azole drug, the growth and development of bougainvillea tuber roots can be further improved, and the maximum individual tuber root thickness of the bougainvillea tuber roots can reach 18-22.5 cm after 34 months of growth.

Description

Technical Field

[0001] The invention relates to the technical field of plant growth regulators, and in particular to a growth promoter for the formation and expansion of tuber-shaped roots of bougainvillea and applications thereof. Background Art

[0002] Bougainvillea is one of the most popular ornamental plants in southern my country. Bougainvillea bonsai is primarily cultivated by potting plants from cuttings for several years, then cultivating them through traditional Chinese bonsai techniques such as shaping and pruning, twisting, root-exposing, and grafting. Exposed-root bonsai are particularly popular because they offer both flower viewing and root viewing.

[0003] In the production of traditional bougainvillea exposed-root bonsai, the root cultivation treatment is generally carried out by adding soil and then lifting the roots year by year, or by using materials such as root controllers to pile up the soil for cultivation. However, this method takes too long, more than 5 years to cultivate, and the part of the roots exposed to the ground is weak and has a single shape, which makes it difficult to reflect the vicissitudes of life and simplicity. Therefore, it is necessary to develop a technology that can make the root system thicker, generate enlarged potato-shaped roots of various shapes, and make the exposed root part more ornamental, so as to promote the industrialization development of exposed-root bougainvillea bonsai.

[0004] At present, in the production technology of bougainvillea exposed-root bonsai, the only compound agent technology for promoting the rapid generation of the various tuber-shaped roots of bougainvillea is the "Rapid Cultivation Method of Bougainvillea Tuber-Root Bonsai Embryo" (CN202010757715.7) by the inventor's own research group. This technology uses potassium dihydrogen phosphate, magnesium sulfate and other agents to quickly generate tuber roots. The application of this technology has now cultivated a large number of bougainvillea bonsai embryo materials. However, there are no public reports on the technical methods for treating the roots of growing ornamental plants with a mixture of multiple fertilizers and components without special fertilizer effects to cultivate the root shape of bougainvillea. In the existing technology for promoting the expansion of plant roots, different components of rhizome enlargers are often used to promote the expansion of underground rhizomes of Chinese medicinal materials such as atractylodes, ginseng, Panax notoginseng, and angelica, as well as potatoes, ginger and garlic, and there are many scientific research results. In terms of patented technologies, such as: "A seed propagation technology of Atractylodes lancea of the Asteraceae family" (CN 202011506502.3), "A method for increasing potato yield during the tuber swelling period" (CN 201610627446.6), and "A method for intercropping Angelica dahurica under a ginkgo forest" (CN201710798006.1), the principle is to use rhizome (tuber) enlargement hormones (agents) in the middle and late stages of growth to promote the conversion of plant vegetative growth to reproductive growth, reduce excessive nutrient consumption due to excessive plant growth, and achieve the purpose of promoting the growth and swelling of rhizomes. However, most of the existing rhizome (tuber) enlargements contain some heavy metal elements that are difficult to remove and easy to remain, which is not conducive to environmental protection; similarly, in the patent "A rapid cultivation method for bougainvillea potato root bonsai embryo materials" (CN202010757715.7), the growth regulator paclobutrazol is used to regulate plant growth, and the dosage requirements of paclobutrazol in practical operations are relatively strict. Excessive dosage can easily cause long-term growth inhibition in the later stage. At the same time, sufficient water is required to ensure growth. Excessive water in the growth process of bougainvillea can easily cause the aboveground part of the plant to grow too long and the underground part to be inhibited. Therefore, it is necessary to develop an environmentally friendly bougainvillea potato root generation and enlargement growth promoter with low operation requirements.

[0005] Therefore, in order to promote the rapid development of the bougainvillea industry and in response to market demand, a growth promoter for bougainvillea potato roots was developed, which can promote the formation of potato-like roots in the plant root system and rapidly grow and expand. Summary of the Invention

[0006] The present invention aims to solve at least one of the technical problems existing in the above-mentioned prior art. To this end, a growth promoter for the formation and expansion of tuber roots of bougainvillea plants and its use are provided. The promoter can promote the formation of tuber roots in the root system of bougainvillea plants and rapidly grow and expand them, so that the maximum thickness of a single tuber root reaches 18-22.5 cm.

[0007] The inventive concept of the present invention is as follows: by adding Bacillus subtilis and seaweed fish protein liquid to the tuber root generator formula and adjusting the type of azole drugs at the same time, adding Bacillus subtilis to the tuber root enlargement agent formula, the promotion and growth development of the tuber roots of Bougainvillea can be further improved, and the maximum single tuber root thickness of the Bougainvillea tuber roots can reach 18-22.5 cm after 34 months of growth.

[0008] A first aspect of the present invention provides a growth promoter for the formation and enlargement of tuber roots of bougainvillea, the growth promoter comprising a tuber root generator and a tuber root enlarger; the tuber root generator comprising potassium dihydrogen phosphate, magnesium sulfate, Bacillus subtilis powder, aminochitosan oligosaccharide, seaweed fish protein liquid, uniconazole, and brassinolide; the tuber root enlarger comprising potassium dihydrogen phosphate, magnesium sulfate, Bacillus subtilis powder, aminochitosan oligosaccharide, uniconazole, and brassinolide.

[0009] Compared with the prior art, the beneficial effects of a growth promoter for the generation and enlargement of tuber-shaped roots of bougainvillea provided in the first aspect of the present invention are as follows: adding Bacillus subtilis and seaweed fish protein liquid to the formula, and adjusting the type of azole drugs at the same time, can further improve the growth and development of the tuber-shaped root tubers of bougainvillea; in addition, the generator and enlargement agent can respectively provide promoters corresponding to different growth stages of bougainvillea, which can further promote the growth and development of the root system of bougainvillea, form tuber-shaped roots, and grow and enlarge rapidly, so that the maximum thickness of the single tuber-shaped root reaches 18-22.5 cm.

[0010] Preferably, the Bacillus subtilis powder is a Bacillus subtilis wettable powder, and the Bacillus subtilis powder has a live spore content of 20-50 billion live spores per gram.

[0011] Preferably, the tuber root generating agent comprises, by weight, 1000-3000 parts of phosphate, 10-100 parts of magnesium sulfate, 8000-15000 parts of Bacillus subtilis powder, 100-500 parts of aminochitosan oligosaccharide, 5000-20000 parts of seaweed fish protein liquid, 10-100 parts of uniconazole, and 0.1-1.5 parts of brassinolide; further preferably, the tuber root generating agent comprises, by weight, 1500-2500 parts of phosphate, 30-60 parts of magnesium sulfate, 10000-15000 parts of Bacillus subtilis powder, 100-300 parts of aminochitosan oligosaccharide, 10000-20000 parts of seaweed fish protein liquid, 50-80 parts of uniconazole, and 0.5-1 part of brassinolide.

[0012] Preferably, the tuber root generator further comprises a solvent, and further preferably, the solvent comprises water.

[0013] Preferably, the concentrations of the components of the tuber-shaped root generator include: 1-3 g / L of phosphate, 10-100 mg / L of magnesium sulfate, 8-15 g / L of Bacillus subtilis powder, 100-500 mg / L of aminochitosan oligosaccharide, 5-20 g / L of seaweed fish protein liquid, 10-100 mg / L of uniconazole, and 0.1-1.5 mg / L of brassinolide; further preferably, the concentrations of the components of the tuber-shaped root generator include: 1.5-2.5 g / L of phosphate, 30-60 mg / L of magnesium sulfate, 10-15 g / L of Bacillus subtilis powder, 100-300 mg / L of aminochitosan oligosaccharide, 10-20 g / L of seaweed fish protein liquid, 50-80 mg / L of uniconazole, and 0.5-1 mg / L of brassinolide.

[0014] Preferably, the tuber root enlargement agent comprises, by weight, 1000-3000 parts of phosphate, 10-100 parts of magnesium sulfate, 5000-15000 parts of Bacillus subtilis powder, 200-600 parts of aminochitosan oligosaccharide, 100-200 parts of uniconazole, and 0.1-1.5 parts of brassinolide; further preferably, the tuber root enlargement agent comprises, by weight, 2000-3000 parts of phosphate, 40-80 parts of magnesium sulfate, 8000-15000 parts of Bacillus subtilis powder, 300-400 parts of aminochitosan oligosaccharide, 100-150 parts of uniconazole, and 0.5-1 part of brassinolide.

[0015] Preferably, the tuber root enlargement agent further comprises a solvent, and further preferably, the solvent comprises water.

[0016] Preferably, the concentrations of the components of the tuber root enlargement agent include: 1-3 g / L of phosphate, 10-100 mg / L of magnesium sulfate, 5-15 g / L of Bacillus subtilis powder, 200-600 mg / L of aminochitosan oligosaccharide, 100-200 mg / L of uniconazole, and 0.1-1.5 mg / L of brassinolide; further preferably, the concentrations of the components of the tuber root enlargement agent include: 2.0-3.0 g / L of phosphate, 40-80 mg / L of magnesium sulfate, 8-15 g / L of Bacillus subtilis powder, 300-400 mg / L of aminochitosan oligosaccharide, 100-150 mg / L of uniconazole, and 0.5-1 mg / L of brassinolide.

[0017] Preferably, the content of the phosphate, the magnesium sulfate, the aminochitosan oligosaccharide and the uniconazole in the tuber root enlargement agent is greater than the content of the phosphate, the magnesium sulfate, the aminochitosan oligosaccharide and the uniconazole in the tuber root generator.

[0018] Preferably, the content of live spores of Bacillus subtilis in the tuber root enlargement agent is greater than the content of live spores of Bacillus subtilis in the tuber root generator.

[0019] The second aspect of the present invention provides a method for preparing the growth promoter, characterized in that it comprises the following steps:

[0020] The tuber root generating agent is prepared by mixing phosphate, magnesium sulfate, Bacillus subtilis powder, aminochitosan oligosaccharide, seaweed fish protein liquid, uniconazole and brassinolide;

[0021] The tuber root enlargement agent is prepared by mixing phosphate, magnesium sulfate, Bacillus subtilis powder, aminochitosan oligosaccharide, uniconazole and brassinolide;

[0022] The tuber root generator and the tuber root enlarger together constitute the growth promoter.

[0023] The third aspect of the present invention provides a plant cultivation method, which includes the following steps: applying the growth promoter to Bougainvillea, first periodically applying the tuber-like root generator, and then periodically applying the tuber-like root enlargement agent.

[0024] A method for cultivating tuber-shaped roots of bougainvillea comprises the following steps: applying the growth promoter to bougainvillea seedlings, first periodically applying the tuber-shaped root generator, and then periodically applying the tuber-shaped root enlarger.

[0025] Preferably, the head diameter of the Bougainvillea seedlings is 2cm-3cm and the seedling age is 1.0-1.5 years.

[0026] Preferably, the tuber root generator is applied by spraying the leaves and branches of Bougainvillea.

[0027] Preferably, the interval time of periodic application of the tuber-shaped root generator is 50-70 days / time, and the number of applications is 5-7 times; further preferably, the application amount of each periodic application of the tuber-shaped root generator is 200-300 mL / plant.

[0028] Preferably, the tuber root enlargement agent is applied by spraying the leaves and branches of Bougainvillea.

[0029] Preferably, the interval of periodic application of the tuber-shaped root enlargement agent is 50-70 days / time, and the number of applications is 3-5 times; further preferably, the dosage of each periodic application of the tuber-shaped root growth agent is 300-500 mL / plant.

[0030] Preferably, the cultivation method comprises the following steps:

[0031] (1) Selection of a cultivation site and a cultivation substrate: The cultivation site is a planting site with full sunlight, and the cultivation substrate is 55-65 wt% sandy loam, 15-25 wt% humus soil, and 15-25 wt% volcanic rock fragments; more preferably, the particle size of the volcanic rock fragments is 1-2 cm;

[0032] (2) Seedling treatment: Select 0.5-1.5 year old seedlings with a head diameter of 2 cm-3 cm and cut them 30-40 cm above the ground; retain 2-5 thick roots with a root length of 5-10 cm and remove messy roots; soak the seedlings in a conventional disinfectant rooting solution for 20-40 minutes, then remove and dry them to prepare treated seedlings; the conventional disinfectant rooting solution contains 40% carbendazim wettable powder 4-6 g / L and naphthaleneacetic acid 300-500 mg / L;

[0033] (3) Cultivation in pots: A container with a diameter of 18-22 cm and a height of 25-27 cm is pre-filled with the cultivation matrix of 8-12 cm in height, and then the treated seedlings are placed in the container, backfilled with the cultivation matrix, compacted, and watered to establish roots; normal cultivation management is carried out, and watering is carried out when the cultivation matrix on the surface of the container is dry and white during management; further preferably, the seedlings are placed at a spacing of (1.2-1.7) m × (1.2-1.7) m per plant;

[0034] (4) Promoting the formation of tuber roots: 25-35 days after entering normal cultivation management, start topdressing and promoting the formation of tuber roots at the roots; every 25-35 days, irrigate with a 600-1000-fold diluted high-phosphorus 12-45-10 water-soluble fertilizer once; 10-20 days after topdressing, start spraying the tuber root generator on the leaves and branches of Bougainvillea. The interval for periodic application of the tuber root generator is 50-70 days / time, the number of applications is 5-7 times, and the application amount is 200-300 mL / plant each time;

[0035] (5) Repotting and root expansion cultivation: When the diameter of the Bougainvillea head is 6-8 cm, the Bougainvillea pile is taken out of the container by breaking the pot. After taking it out, the upright branches are trimmed according to the shape, and only the primary branches and secondary branches sprouting from the main trunk are retained, and the length is 4-6 cm. The height of the whole tree is controlled below 40-60 cm. At the same time, only the roots that are expanded into potato-like shapes are retained, and the redundant and messy roots are removed; a container with a diameter of 25-30 cm and a height of 34-40 cm is selected and filled with the above-mentioned 15-25 cm high Cultivate the substrate, place the pruned bougainvillea in the container and bury it shallowly, backfill the cultivation substrate, compact it, and water it to establish roots. Water it daily when the cultivation substrate on the surface of the container is dry and white; after changing the container, irrigate it with a high-phosphorus 12-45-10 water-soluble fertilizer diluted 800 times once every 28-32 days; after changing the container, spray the leaves and branches with a compound drug that promotes tuber root enlargement once every 50-70 days, the number of applications is 3-5, and the application amount is 300-500 mL / plant;

[0036] (6) Finished product processing: 20-24 months after changing the container, remove the bougainvillea stump by breaking the pot, clean it, trim off the fine roots on the top of each potato-shaped root and only keep the small roots at the bottom to obtain the bougainvillea potato-shaped root.

[0037] The overall operation process of the scheme of the present invention is simple and easy to operate, and workers engaged in flower and seedling production can carry out the operation independently, thereby reducing labor costs.

[0038] On the other hand, the present invention also provides the growth promoter for use in the cultivation of the tuber-shaped roots of Bougainvillea truncatula with strong ornamental value.

[0039] Compared with the prior art, the present invention has the following beneficial effects:

[0040] (1) Adding Bacillus subtilis and seaweed fish protein liquid to the formula and adjusting the type of azole drugs can further improve the growth and development of the tuber roots of Bougainvillea; in addition, the generator and the swelling agent can respectively provide promoters corresponding to the different growth stages of Bougainvillea, which can further promote the growth and development of the root system of Bougainvillea, form tuber roots, and grow and swell rapidly, so that the maximum thickness of the single tuber root reaches 18-22.5 cm.

[0041] (2) At different stages, fertilizers such as phosphate, magnesium sulfate, aminochitosan oligosaccharide and seaweed fish protein solution with appropriate concentrations that can effectively promote the growth and development of the root system of Bougainvillea and the formation of tuber roots are used, and an appropriate concentration of closazone is added to inhibit the elongation of the aboveground branches and stems. At the same time, Bacillus subtilis is added in two stages to improve soil fertility, improve the utilization rate of chemical fertilizers, promote the transfer of nutrients from the growth of stem nodes to the underground, and promote the formation and expansion of tuber roots. At the same time, brassinolide is used to accelerate the rapid division and elongation of cells and increase the growth rate of tuber roots, so as to achieve the cultivation requirements of products with good ornamental effects.

[0042] (3) This technical solution uses the growth regulator uniconazole, which is easy to decompose and has no obvious inhibitory effect on later growth, to control the growth of the aboveground part. Its dosage is relatively loose, which can avoid excessive growth caused by excessive water supply in daily management. At the same time, the added Bacillus subtilis and brassinolide are both plant extracts and do not contain difficult-to-decompose heavy metal elements, which is beneficial to environmental protection. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Figure 1 This is a physical picture of the tuber-shaped roots of Bougainvillea cultivated in Example 1;

[0044] Figure 2 This is a physical picture of the tuber-shaped roots of Bougainvillea cultivated in Example 2;

[0045] Figure 3 、 Figure 4 This is a physical picture of the tuber-shaped roots of Bougainvillea cultivated in Example 3. DETAILED DESCRIPTION

[0046] In order to make the technical solution of the present invention more clearly understood by those skilled in the art, the following examples are given for illustration. It should be noted that the following examples do not limit the scope of protection claimed by the present invention.

[0047] Unless otherwise specified, the raw materials, reagents, or devices used in the following examples can be obtained from conventional commercial sources or by existing known methods.

[0048] Rooting watering refers to the first watering after transplanting, which can make the roots and soil in close contact, which is beneficial for the roots to absorb water and nutrients from the soil.

[0049] The average head diameter thickness in the present invention refers to the average stem diameter of the Bougainvillea plant.

[0050] The aminochitosan oligosaccharide of the present invention was purchased from Henan Zhuogu Biotechnology Co., Ltd., the Bacillus subtilis wettable powder was purchased from Hubei Jiufenglong Chemical Co., Ltd., and the seaweed fish protein liquid was purchased from Shandong Zhenlumei Biotechnology Co., Ltd.

[0051] Example 1

[0052] The growth promoter of Example 1 includes a tuber-shaped root generator and a tuber-shaped root enlarger.

[0053] The growth promoter of Example 1 includes a tuber root generator and a tuber root enlargement agent. The solvent of the tuber root generator is water. The concentrations of the components are 1.5 g / L of potassium dihydrogen phosphate, 30 mg / L of magnesium sulfate, 10 g / L of 20 billion / g Bacillus subtilis wettable powder, 200 mg / L of aminochitosan oligosaccharide, 10 g / L of seaweed fish protein solution, 50 mg / L of uniconazole, and 0.5 mg / L of brassinolide. The amount of water used is 1 L.

[0054] The solvent of the tuber root enlargement agent is water, and the concentrations of each component are 2g / L of potassium dihydrogen phosphate, 40mg / L of magnesium sulfate, 8g / L of 40 billion / g of Bacillus subtilis wettable powder, 400mg / L of aminochitosan oligosaccharide, 100mg / L of uniconazole, and 0.5mg / L of brassinolide. The amount of water used is 1L.

[0055] The preparation method of the growth promoter comprises the following steps:

[0056] The tuber root generating agent is prepared by mixing phosphate, magnesium sulfate, Bacillus subtilis powder, aminochitosan oligosaccharide, seaweed fish protein liquid, uniconazole, brassinolide and water;

[0057] The tuber root enlargement agent is prepared by mixing phosphate, magnesium sulfate, Bacillus subtilis powder, aminochitosan oligosaccharide, uniconazole, brassinolide and water;

[0058] Tuberose root generators and tuberose root enlargers together constitute growth promoters.

[0059] A method for cultivating bougainvillea tuber-shaped roots using a growth promoter for tuber-shaped root formation and expansion, the method comprising the following steps:

[0060] The implemented variety is the "BBK" variety, with 100 plants, and the experimental time is from February 2018 to August 2021.

[0061] (1) Preparation of cultivation site and cultivation substrate:

[0062] a. Choose a planting site with full sunlight and high sand content, and cover it with ground cloth;

[0063] b. The cultivation medium is 60% sandy loam, 20% humus soil, and 20% 1-2cm volcanic rock fragments, and the three are evenly mixed;

[0064] (2) Bougainvillea seedling treatment:

[0065] Seedling treatment: Select one-year-old seedlings with a head diameter of 2-3 cm and cut them 30-40 cm above the ground. Retain only 2-5 thick roots, 5-10 cm long, and remove any loose roots. Soak the seedlings in a conventional rooting disinfectant solution for 30 minutes, then remove and dry before planting. The conventional rooting disinfectant solution should consist of 5g / L 40% carbendazim wettable powder and 400mg / L naphthaleneacetic acid.

[0066] (3) Cultivation in pots:

[0067] a. Use a 200mm diameter 260mm high plastic pot as the potting container. Pre-fill the plastic pot with 10cm of culture medium. Then place the treated seedlings into the pot, backfill with culture medium, compact it, and water it enough to establish roots. After potting, place the seedlings in rows with a spacing of 1.5m x 1.5m per plant.

[0068] b. Enter normal cultivation and management, and water only when the substrate on the pot surface is dry and white;

[0069] (4) Promote the formation of tuber roots:

[0070] a. One month after potting and entering normal cultivation and management, start topdressing and promoting the formation of tuberous roots;

[0071] b. Topdress with 800-fold diluted high-phosphorus 12-45-10 water-soluble fertilizer once every 30 days;

[0072] c. Prepare a tuber root generator consisting of 1.5g / L potassium dihydrogen phosphate, 30mg / L magnesium sulfate, 10g / L 20 billion / g Bacillus subtilis wettable powder, 200mg / L aminochitosan oligosaccharide, 10g / L seaweed fish protein solution, 50mg / L uniconazole, and 0.5mg / L brassinolide, with 1L of tap water as the solvent;

[0073] d. 15 days after the first topdressing, spray the leaves and branches with a tuber-shaped root generator once, 2 months apart, for 6 consecutive sprays at a rate of 200 mL per plant.

[0074] (5) Repotting and root expansion cultivation:

[0075] a. In the 12th month after potting (February 2019), when the diameter of the Bougainvillea head is about 6-8 cm, remove the Bougainvillea stump from the plastic pot by breaking the pot and replace it with a larger pot as the potting container for replanting; after removing it, trim the upright branches according to the initial bonsai embryo shape, retaining only the primary and secondary branches sprouting from the main trunk, all of which are 4-6 cm in length, and control the height of the entire tree below 40-60 cm. At the same time, only retain the roots that have expanded into potato-shaped roots, and remove the excess messy roots;

[0076] b. Use a 260mm diameter 350mm high plastic pot as the upper pot container, pre-fill the plastic pot with 20cm high cultivation substrate, then place the treated bougainvillea into the pot and bury it shallowly, backfill the cultivation substrate, compact it, and water it enough to establish roots; the cultivation substrate uses the same substrate as in step 1; place it in the original position and enter the usual cultivation management. Usually, water it when the substrate on the pot surface is dry and white.

[0077] c. Topdress with 12-45-10 water-soluble fertilizer diluted 800 times with high phosphorus content once every 30 days;

[0078] d. Prepare a tuber root enlargement agent consisting of 2g / L potassium dihydrogen phosphate, 40mg / L magnesium sulfate, 8g / L 40 billion / g Bacillus subtilis wettable powder, 400mg / L aminochitosan oligosaccharide, 100mg / L uniconazole, and 0.5mg / L brassinolide, with 1L of tap water as the solvent;

[0079] e. Spray the leaves and branches with a root enlargement agent once every two months for four times in a row, with a dosage of 300 mL per plant;

[0080] (6) Finished product processing:

[0081] a. 22 months after repotting and root expansion (December 2020), remove the bougainvillea stump again by breaking the pot, and use a high-pressure water gun to clean the soil and volcanic rocks attached to the tuberous roots. Prune off the fine roots on the top of each tuberous root, leaving only the small roots at the bottom.

[0082] b. At this point, the cultivation of Bougainvillea with multiple tuber-shaped roots of different shapes and specifications has been completed.

[0083] The average head diameter, average number of tuber roots, tuber setting rate, maximum single tuber root thickness, and overall ornamental effect of the tuber-shaped root bougainvillea cultivated using the inventive technology were statistically analyzed and recorded in Table 1.

[0084] Figure 1 This is a physical picture of the tuber-shaped roots of Bougainvillea cultivated in Example 1.

[0085] Example 2

[0086] The difference between Example 2 and Example 1 is that the concentrations of the components of the tuber root generator and the tuber root enlarger are different, and the experimental time is from June 2018 to December 2021:

[0087] The solvent of the tuber root generator is water, and the concentrations of the components are 2g / L potassium dihydrogen phosphate, 50mg / L magnesium sulfate, 10g / L 20 billion / g Bacillus subtilis wettable powder, 200mg / L aminochitosan oligosaccharide, 15g / L seaweed fish protein liquid, 60mg / L uniconazole, and 0.8mg / L brassinolide. The amount of water used is 1L.

[0088] The solvent of the tuber root enlargement agent is water, and the concentrations of each component are 2.5g / L of potassium dihydrogen phosphate, 60mg / L of magnesium sulfate, 10g / L of 40 billion / g of Bacillus subtilis wettable powder, 300mg / L of aminochitosan oligosaccharide, 120mg / L of uniconazole, and 0.8mg / L of brassinolide. The amount of water used is 1L.

[0089] The average head diameter, average number of tuber roots, tuber setting rate, maximum single tuber root thickness, and overall ornamental effect of the tuber-shaped root bougainvillea cultivated using the inventive technology were statistically analyzed and recorded in Table 1.

[0090] Figure 2 This is a physical picture of the tuber-shaped roots of Bougainvillea cultivated in Example 2.

[0091] Example 3

[0092] The difference between Example 3 and Example 1 is that the concentrations of the components of the tuber root generator and the tuber root enlarger are different, and the experimental time is from December 2018 to May 2022:

[0093] The solvent of the tuber root generator is water, and the concentrations of the components are 2.5g / L potassium dihydrogen phosphate, 60mg / L magnesium sulfate, 15g / L 20 billion / g Bacillus subtilis wettable powder, 200mg / L aminochitosan oligosaccharide, 20g / L seaweed fish protein liquid, 80mg / L uniconazole, and 1mg / L brassinolide. The amount of water used is 1L.

[0094] The solvent of the tuber root enlargement agent is water, and the concentrations of each component are 3g / L of potassium dihydrogen phosphate, 80mg / L of magnesium sulfate, 15g / L of 40 billion / g of Bacillus subtilis wettable powder, 400mg / L of aminochitosan oligosaccharide, 150mg / L of uniconazole, and 1mg / L of brassinolide. The amount of water used is 1L.

[0095] The average head diameter, average number of tuber roots, tuber setting rate, maximum single tuber root thickness, and overall ornamental effect of the tuber-shaped root bougainvillea cultivated using the inventive technology were statistically analyzed and recorded in Table 1.

[0096] Figure 3 、 Figure 4 This is a physical picture of the tuber-shaped roots of Bougainvillea cultivated in Example 3.

[0097] Comparative Example 1

[0098] The existing method of conventional bonsai soil cultivation and root induction was adopted, without potting cultivation operations. 100 bougainvilleas were directly cultivated on high ridges in the field for 3 years and then transplanted. The planting period was from February 2018 to May 2022.

[0099] The average head diameter, average number of tuber roots, tuber setting rate, maximum single tuber root thickness, and overall ornamental effect of the tuber-shaped root bougainvillea cultivated using the inventive technology were statistically analyzed and recorded in Table 1.

[0100] Comparative Example 2

[0101] The difference between Comparative Example 2 and Example 1 is that the tuber root enlargement agent is replaced with tap water, and the planting time is from February 2019 to August 2021.

[0102] The average head diameter, average number of tuber roots, tuber setting rate, maximum single tuber root thickness, and overall ornamental effect of the tuber-shaped root bougainvillea cultivated using the inventive technology were statistically analyzed and recorded in Table 1.

[0103] Comparative Example 3

[0104] The difference between Comparative Example 3 and Example 1 is that the 20 billion / g Bacillus subtilis wettable powder in the tuber root generator is replaced with seaweed fish protein liquid, that is, no Bacillus subtilis wettable powder is added and the content of seaweed fish protein liquid is increased to 20 g / L.

[0105] The average head diameter, average number of tuber roots, tuber setting rate, maximum single tuber root thickness, and overall ornamental effect of the tuber-shaped root bougainvillea cultivated using the inventive technology were statistically analyzed and recorded in Table 1.

[0106] Comparative Example 4

[0107] The difference between Comparative Example 4 and Example 1 is that the seaweed fish protein liquid in the tuber root generator is replaced with 20 billion / g Bacillus subtilis wettable powder, that is, no seaweed fish protein liquid is added and the content of 20 billion / g Bacillus subtilis wettable powder is increased to 20 g / L.

[0108] The average head diameter, average number of tuber roots, tuber setting rate, maximum single tuber root thickness, and overall ornamental effect of the tuber-shaped root bougainvillea cultivated using the inventive technology were statistically analyzed and recorded in Table 1.

[0109] Table 1 Planting results of each embodiment and comparative example

[0110]

[0111]

[0112] As shown in the table, comparative example 1 was cultivated using the existing method of conventional bonsai soiling and root induction. During the cultivation process, the whole plant retained branches and leaves, grew vigorously, and its average head diameter reached 13.6 cm, but the tuber rate was only 1.2%, the maximum single tuber root thickness was only 2.9 cm, and the average number of tuber roots was only 0.6; comparative example 2 used a compound agent to promote tuber root generation, and was not treated with a compound agent to promote tuber root expansion. The tuber rate was 87.4%, the maximum tuber root thickness was 5.3 cm, and the average number of tuber roots was 6.7. In comparison, the tuber rate was as low as 93.3% and as high as 99.7% under the three different agent concentration ratios of the method of the present invention, the maximum tuber root thickness reached 22.5 cm, and the average number of tuber roots was as low as 6.8.

[0113] The above experimental data show that the growth promoter for the formation and expansion of tuber-shaped roots of Bougainvillea of the present invention is effective, can promote the formation and expansion of tuber-shaped roots, is suitable as a method for preparing embryonic materials for Bougainvillea exposed-root bonsai, and can quickly improve the ornamental value of its roots.

Claims

1. A method for cultivating tuber-shaped roots of Bougainvillea, characterized in that: The cultivation method comprises the following steps: applying a growth promoter to bougainvillea seedlings, wherein the growth promoter is applied by spraying the leaves and branches of the bougainvillea; The tuber root generator is first applied periodically, and then the tuber root enlargement agent is applied periodically; The interval of periodic application of the tuber root growth agent is 50-70 days / time, the number of applications is 5-7 times, and the amount of each application is 120-150 mL / plant; the interval of periodic application of the tuber root enlargement agent is 50-70 days / time, the number of applications is 3-5 times, and the amount of each application is 150-200 mL / plant; The growth promoter includes a tuber root generator and a tuber root enlargement agent; The tuber root generator comprises, by weight, 1000-3000 parts of potassium dihydrogen phosphate, 10-100 parts of magnesium sulfate, 8000-15000 parts of Bacillus subtilis powder, 100-500 parts of aminochitosan oligosaccharide, 5000-20000 parts of seaweed fish protein solution, 10-100 parts of uniconazole, and 0.1-1.5 parts of brassinolide. The tuber root enlargement agent comprises, by weight, 1000-3000 parts of potassium dihydrogen phosphate, 10-100 parts of magnesium sulfate, 5000-15000 parts of Bacillus subtilis powder, 200-600 parts of aminochitosan oligosaccharide, 100-200 parts of uniconazole, and 0.1-1.5 parts of brassinolide.

2. The cultivation method according to claim 1, wherein The Bacillus subtilis powder is a Bacillus subtilis wettable powder, and the live spore content of the Bacillus subtilis powder is 20-50 billion live spores / gram.

3. The cultivation method according to claim 1, wherein The tuber root generator also includes a solvent, and the concentrations of the components include: 1-3 g / L of potassium dihydrogen phosphate, 10-100 mg / L of magnesium sulfate, 8-15 g / L of Bacillus subtilis powder, 100-500 mg / L of aminochitosan oligosaccharide, 5-20 g / L of seaweed fish protein liquid, 10-100 mg / L of uniconazole, and 0.1-1.5 mg / L of brassinolide.

4. The cultivation method according to claim 1, characterized in that The tuber root enlargement agent also includes a solvent, and the concentrations of the components include: 1-3 g / L of potassium dihydrogen phosphate, 10-100 mg / L of magnesium sulfate, 5-15 g / L of Bacillus subtilis powder, 200-600 mg / L of aminochitosan oligosaccharide, 100-200 mg / L of uniconazole, and 0.1-1.5 mg / L of brassinolide.

5. The cultivation method according to claim 1, characterized in that The contents of the potassium dihydrogen phosphate, the magnesium sulfate, the aminochitosan oligosaccharide and the uniconazole in the tuber root enlargement agent are respectively greater than the contents of the potassium dihydrogen phosphate, the magnesium sulfate, the aminochitosan oligosaccharide and the uniconazole in the tuber root generator.

6. The cultivation method according to any one of claims 1 to 5, characterized in that The preparation method of the growth promoter comprises the following steps: The tuber root generating agent is prepared by mixing potassium dihydrogen phosphate, magnesium sulfate, Bacillus subtilis powder, aminochitosan oligosaccharide, seaweed fish protein liquid, uniconazole and brassinolide; The tuber root enlargement agent is prepared by mixing potassium dihydrogen phosphate, magnesium sulfate, Bacillus subtilis powder, aminochitosan oligosaccharide, uniconazole and brassinolide; The tuber root generator and the tuber root enlarger together constitute the growth promoter.

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