A plant cultivation system and method comprising root microorganisms

Abstract

The present application relates to a kind of plant cultivation system and method comprising root microorganism, comprising: collection module, first detection unit, second detection unit, sterilization control unit and regulation unit.The harmful organism includes the first type of harmful organism with primary pathogenicity to the plant planted in cultivation system, secondary pathogenicity of the second type of harmful organism and tertiary pathogenicity of the third type of harmful organism, the regulation unit is configured as: when the detected harmful organism is the first type of harmful organism, control the sterilization control unit disinfects the cultivation system, wherein, when the category and concentration of first type of harmful organism exceed the first threshold, control the sterilization control unit with first disinfection mode disinfects the cultivation system;When the category and concentration of first type of harmful organism are lower than the first threshold, control the sterilization control unit with second disinfection mode disinfects the cultivation system.

Description

Technical Field

[0001] This invention relates to the field of crop cultivation technology, to plant cultivation systems and methods, and more particularly to a plant cultivation system and method comprising root microorganisms. Background Technology

[0002] Currently, hydroponics is a common breeding method for cultivating crops, especially vegetables such as lettuce, celery, tomatoes, and cucumbers. Hydroponic systems are also used for fruits and flowers, such as melons, blueberries, and strawberries, as well as flowers like orchids, daffodils, and hyacinths. The nutrient solution supplied in hydroponic systems is recycled. Hydroponic systems are difficult to clean, and the temperature, humidity, and nutrient conditions are ideal for microbial growth. However, repeatedly used nutrient solutions can harbor harmful organisms such as pathogens, nematodes, and planktonic plants. The root system is the most important part of a plant, serving as a crucial habitat for microorganisms to gather, live, and reproduce. Monitoring root microorganisms is crucial for plant growth and development, as well as for the biological control of pests and diseases. When plants are infected, they exhibit water imbalances, often manifesting as wilting or wilt. In current hydroponic system management, once plant infection or the presence of harmful organisms near the roots is detected, disinfectants are sprayed to eliminate the harmful organisms.

[0003] For example, the invention patent with authorization number CN103159528B provides a nutrient solution and method for producing potato seed stock by aeroponics. The seedling management method is as follows: observe the leaf color of the aeroponic seedlings daily to see if they are normal, if there are any disease spots, or if they are wilting; check if the root system of the seedlings is normal; remove any fallen potatoes or other debris in the container in time; prevent excessive growth of the seedlings during the middle stage of growth, which will affect tuber formation; prevent fungal diseases such as early blight and late blight, and bacterial diseases such as ring rot and bacterial wilt in aeroponic potatoes; apply fungicide every two weeks and insecticide every two weeks; or add an appropriate amount of broad-spectrum fungicide to the nutrient solution regularly; once diseased plants are found, they should be removed immediately and treated with pesticides. This invention provides a method for regular fungicide and insecticide application.

[0004] However, commonly used disinfectants are toxic to plants in hydroponic systems. Applying disinfectants when plants are free of pests and diseases can actually harm their growth. Furthermore, root microorganisms include organisms that are harmful, harmless, and beneficial to plant growth. Some root microorganisms promote plant growth, such as increasing nitrogen and phosphorus absorption and enhancing drought and bending resistance. In addition, plants can regulate the root environment through root exudates to promote the growth of root microorganisms, and roots also contain beneficial bacteria that promote the secretion of surfactants and plant growth hormones. While existing hydroponic systems have disinfection programs, they lack water quality monitoring and fail to develop targeted disinfection methods or procedures based on the actual pollution levels of the water where the plant roots are located. This results in the affected types and amounts of microorganisms near the plant roots, preventing them from exerting their beneficial effects. In particular, the microorganisms in water bodies can vary, such as the presence of harmful but not beneficial microorganisms, harmless but not beneficial microorganisms, or harmless but beneficial microorganisms. Hydroponic systems cannot implement targeted disinfection methods or procedures, which can prevent plants from achieving normal or favorable growth conditions, greatly reducing production efficiency and wasting production resources. Therefore, it is necessary to develop targeted disinfection procedures or methods based on the types and contents of microorganisms near the rhizosphere in the water body to ensure that plants are in a high growth efficiency.

[0005] Furthermore, on the one hand, there are differences in understanding among those skilled in the art; on the other hand, the applicant studied a large number of documents and patents when making this invention, but due to space limitations, not all details and contents were listed in detail. However, this does not mean that the present invention does not possess the features of these prior art. On the contrary, the present invention already possesses all the features of the prior art, and the applicant reserves the right to add relevant prior art to the background art. Summary of the Invention

[0006] While existing hydroponic plant systems include disinfection programs, they lack monitoring of the water body and fail to develop targeted disinfection methods or programs based on the actual pathogen infestation or pest / disease status of the water where the plant roots are located. This results in the impact on the types and amounts of microorganisms near the plant roots. In particular, beneficial microorganisms provide nutrients for plant growth, secreting growth stimulants and vitamins to promote growth. When the microorganisms near the roots are predominantly beneficial and few harmful microorganisms, if the hydroponic system still disinfects the plants according to the set disinfection program, the disinfectant has a significant impact on the types and amounts of beneficial microorganisms. Although it can eliminate harmful microorganisms, trace amounts of these microorganisms may not affect plant growth. Disinfection, on the other hand, has a greater impact on beneficial microorganisms, preventing them from fulfilling their beneficial functions near the roots in the water. In reality, this reduces plant growth efficiency. Furthermore, the pathogenicity of harmful organisms varies, and their types and abundance have different effects on plant growth. Some harmful organisms, such as highly pathogenic fungi, proliferate rapidly through various propagules, such as spores, conidia, and chlamydospores, which germinate quickly and can disperse in the air to infect large areas of plants. These fungi directly invade the plant's surface cells, causing rapid disease development. Conversely, some harmful organisms have weaker pathogenicity, and after invasion, their colonization, sporulation, and spore germination are slow, resulting in a slower pathogenic process. The existing disinfection procedures for hydroponic systems are too simplistic in their criteria and methods for determining whether disinfection is necessary, failing to provide targeted disinfection based on the actual situation of the organisms present in the water.

[0007] To address the shortcomings of existing technologies, this invention provides a plant cultivation system comprising root microorganisms, including:

[0008] The detection module is configured to detect microbial-related information in water samples used for hydroponic plants within the plant cultivation system.

[0009] The control unit is configured to adjust the water environment of the plant cultivation system based on microbial-related information detected by the detection module, so as to promote the growth of beneficial microorganisms and inhibit the growth of harmful microorganisms.

[0010] Preferably, the control unit is configured as follows:

[0011] When the microbial-related information indicates that the water sample of the hydroponic plant has a number and concentration of harmful microorganisms with Class I pathogenicity exceeding a first threshold, the water sample of the cultivation system is disinfected in a first mode.

[0012] Preferably, the primary pathogenicity refers to a strong pathogenicity that can rapidly cause disease or death in the plants of the plant cultivation system.

[0013] Preferably, the first mode refers to applying disinfectant to the plant cultivation system in a mode of disinfection every 3 to 7 days, each disinfection lasting 10 to 20 minutes, with a concentration of 5 to 30 mg / L.

[0014] The first threshold is preferably a critical value for the types and concentrations of harmful microorganisms with first-order pathogenicity that cause major diseases or plant death in the hydroponic plants.

[0015] Preferably, the control unit is configured as follows:

[0016] When the microbial-related information indicates that the water sample of the hydroponic plant has a type and concentration of harmful microorganisms with primary pathogenicity below the first threshold, the water sample of the cultivation system is disinfected in a second mode.

[0017] Preferably, the second mode refers to applying disinfectant to the plant cultivation system in a mode of disinfection every 8 to 15 days, each disinfection lasting 10 to 20 minutes, at a concentration of 5 to 30 mg / L.

[0018] Preferably, the control unit is configured as follows:

[0019] When the information related to microorganisms indicates that the water sample from the hydroponic plant contains harmful microorganisms with secondary pathogenicity exceeding a second threshold, the control detection module acquires information related to beneficial microorganisms, wherein...

[0020] When the types and concentrations of the beneficial microorganisms exceed a third threshold, the water sample from the cultivation system is disinfected in a third mode.

[0021] Preferably, the secondary pathogenicity refers to the pathogenicity that causes diseases in the plant cultivation system for a long time with low disease severity.

[0022] Preferably, the third mode refers to applying disinfectant to the plant cultivation system in a mode of disinfection every 8 to 15 days, each disinfection lasting 2 to 10 minutes, with a concentration of 1 to 5 mg / L.

[0023] The second threshold is preferably a critical value for the types and concentrations of harmful microorganisms with secondary pathogenicity that pose a significant threat to the growth of the hydroponic plants.

[0024] The third threshold is preferably a critical value for the types and concentrations of beneficial microorganisms that promote the growth of the hydroponic plants or are beneficial to the growth efficiency of the hydroponic plants.

[0025] Preferably, the control unit is configured as follows:

[0026] When the information related to microorganisms indicates that the water sample from the hydroponic plant contains harmful microorganisms with secondary pathogenicity exceeding a second threshold, the control detection module acquires information related to beneficial microorganisms, wherein...

[0027] When the types and concentrations of the beneficial microorganisms are below the third threshold, the water sample from the cultivation system is disinfected in the fourth mode.

[0028] Preferably, the fourth mode refers to applying disinfectant to the plant cultivation system in a mode of disinfection every 8 to 15 days, each disinfection lasting 2 to 10 minutes, with a concentration of 5 to 30 mg / L.

[0029] Preferably, the plant cultivation system further includes:

[0030] The acquisition module is configured to collect water samples from the cultivation system.

[0031] The disinfection control unit is configured to adjust the disinfection mode based on the analysis results of the control unit.

[0032] The control unit can analyze the microbial generation results in the water sample, wherein...

[0033] The analysis results refer to:

[0034] Based on the signals collected by the detection module, the control unit can control the disinfection control unit to generate a corresponding disinfection mode according to the contribution of microorganisms in the water sample to the plants.

[0035] Preferably, the detection module includes:

[0036] The first detection unit is configured to detect first information related to harmful organisms in the water sample;

[0037] The harmful organisms include Class I harmful organisms with primary pathogenicity to the plants grown in the cultivation system, Class II harmful organisms with secondary pathogenicity, and Class III harmful organisms with tertiary pathogenicity.

[0038] Preferably, the detection module further includes:

[0039] The second detection unit is configured to detect information related to beneficial microorganisms in the water sample.

[0040] Preferably, the acquisition module periodically collects water samples from the area near the plant roots as the detection target for the first detection unit and / or the second detection unit. Preferably, the acquisition cycle can be 2 days / time, 5 days / time, 7 days / time, or 10 days / time.

[0041] Preferably, the cultivation system further includes:

[0042] A temperature control unit configured to control the ambient temperature of the cultivation system;

[0043] An oxygen content control unit is configured to control the oxygen content of the environment of the cultivation system;

[0044] A lighting control unit, configured to control the illumination of the cultivation system.

[0045] The temperature control unit, the oxygen control unit, and the lighting control unit can directionally adjust the environmental conditions of the cultivation system to reduce the spread rate of pathogenic microorganisms.

[0046] The present invention also provides a plant cultivation method comprising root microorganisms, including the following steps:

[0047] Obtain water samples from the cultivation system;

[0048] Obtain information related to microorganisms in the water sample;

[0049] The aquatic environment of the plant cultivation system is adjusted based on information related to microorganisms.

[0050] Preferably, the pests include Class I pests with primary pathogenicity to the plants grown in the cultivation system, Class II pests with secondary pathogenicity, and Class III pests with tertiary pathogenicity.

[0051] The beneficial effects of this invention are:

[0052] 1. This invention selects different disinfection modes based on the actual presence of harmful organisms and beneficial microorganisms in the water. It maximizes the survival of beneficial microorganisms while ensuring plant survival, minimizing the impact of disinfection on the plants themselves. Furthermore, when harmful organisms with primary pathogenicity are detected, the disinfection mode is directly activated without further detection of beneficial microorganisms. The second detection unit is only activated when the type and concentration / abundance of harmful organisms with secondary pathogenicity exceed a second threshold. Highly pathogenic first-class harmful organisms have a significant impact on plants, and the disease progression is rapid after infection. Even the presence of beneficial microorganisms may not be sufficient to resist the harm caused by highly pathogenic microorganisms. Therefore, in this case, the primary objective is to eliminate highly pathogenic microorganisms; that is, when first-class harmful organisms are detected, the elimination of first-class harmful organisms is prioritized without detecting the presence of beneficial microorganisms. When the first type of pests is in a dominant reproductive position, they reproduce rapidly, produce a large number of spores, and have a strong ability to spread spores and a fast spore germination rate. When this happens, the primary goal is to quickly reduce and eliminate the types and concentrations of the first type of pests in the cultivation system. Otherwise, their spores will reproduce and germinate rapidly, and the degree of harm to the plant will increase rapidly, leading to the plant's death.

[0053] 2. When the second type of pests are in a dominant reproductive position, although the pathogenicity of the second type of pests is moderate (weak reproductive capacity / slow germination), the plant is at risk of death if their types and concentrations exceed the second threshold. Even if beneficial microorganisms have the function of stimulating the plant's immune system, the limited types and concentrations of beneficial microorganisms are insufficient to resist the damage to the plant caused by the second type of pests compared to the large number and high concentrations of the second type of pests. In this case, controlling the damage of the second type of pests to plant growth is the primary objective.

[0054] 3. When the third category of pests is in a dominant reproductive position, although the third category of pests are pests, they do not have the ability to cause disease to the plants grown in the cultivation system. For example, some specialized pathogenic microorganisms are used in the cultivation system to grow tomatoes, but the pathogenic microorganism detected is Fusarium oxysporum sesame specialized type, which will not cause disease in tomatoes. Disinfection under these growing conditions may have an adverse effect on the plants themselves. Therefore, when the third category of pests is in a dominant reproductive position, disinfection is not necessary.

[0055] This invention includes a first detection unit to detect information related to harmful organisms. Based on their pathogenicity to the plants grown in the cultivation system, harmful organisms are classified into three categories: Category I, Category II, and Category III. When the number and quantity of Category I harmful organisms exceed a first threshold, the control unit activates a first disinfection mode. When the number and quantity of Category I harmful organisms are below the first threshold, the control unit activates a second disinfection mode. When the number and concentration / abundance of Category II harmful organisms are below the second threshold, the disinfection control unit does not activate a disinfection mode. When the number and concentration / abundance of Category II harmful organisms exceed the second threshold, the control unit activates a second detection unit to obtain information related to beneficial microorganisms, including their types and quantities. If the number and quantity of Category II harmful organisms exceed the second threshold, and the number and concentration / abundance of beneficial microorganisms exceed a third threshold, the control unit activates a third disinfection mode. If the number and concentration / abundance of beneficial microorganisms are below the third threshold, the control unit activates a fourth disinfection mode.

[0056] This invention selects different disinfection modes based on the actual presence of harmful and beneficial microorganisms in the water. It maximizes the survival of beneficial microorganisms while minimizing the impact of disinfection on the plants, ensuring plant survival. Furthermore, it directly activates the disinfection mode when highly pathogenic first-class harmful organisms are detected, without needing to detect a second signal of beneficial microorganisms. The second detection unit is only activated when the type and concentration / abundance of second-class harmful organisms exceed a second threshold. Highly pathogenic first-class harmful organisms have a significant impact on plants, causing rapid disease progression after infection. Even the presence of beneficial microorganisms may not be sufficient to resist the damage caused by highly pathogenic microorganisms. Therefore, eliminating highly pathogenic microorganisms is the primary objective. The impact of moderately pathogenic second-class harmful organisms on plants is relatively lower. Therefore, when second-class harmful organisms exceed the second threshold, the disinfection mode is selected based on the presence of beneficial microorganisms. This targeted disinfection is more beneficial to plant growth. Moreover, activating the second detection unit only in certain situations reduces detection difficulty, shortens the detection process, and improves detection efficiency. Attached Figure Description

[0057] Figure 1 This is a simplified schematic diagram of the module connection relationship of a cultivation system according to a preferred embodiment of the present invention.

[0058] List of reference numerals

[0059] 100: Plant; 200: Water sample; 300: Acquisition module; 400: Temperature control unit; 500: Oxygen content control unit; 600: Lighting control unit; 700: Regulation unit; 710: Disinfection control unit; 800: First detection unit; 900: Second detection unit. Detailed Implementation

[0060] The following is a detailed explanation with reference to the accompanying drawings.

[0061] In this application, information related to harmful organisms refers to information about harmful organisms present in the aquatic environment where the roots of the plants 100 grown in the cultivation system are located, which hinder or are detrimental to the growth of the plants 100. This includes the types and concentrations of harmful organisms, which can be pathogenic microorganisms, planktonic plants 100, etc. Different plants 100 have different effects on harmful organisms. The same pathogenic microorganism may be highly pathogenic to one crop but not pathogenic or have a minor impact on another crop. In this application, primary pathogenicity refers to the strong pathogenicity that can rapidly cause disease or death in the plant 100 of the plant cultivation system. For example, specialized plant pathogens are highly pathogenic to this plant 100. First-class pests with primary pathogenicity have a short infection process, rapid spread, and strong pathogenicity. For instance, *Fusarium oxysporum* tomato specialized form can cause large-scale tomato wilt, resulting in severe disease. Second-class pathogenicity refers to the pathogenicity that causes disease in the plant 100 of the plant cultivation system over a longer period with lower disease severity. The infection process of second-class pests with secondary pathogenicity is significantly different from that of first-class pests. Third-level pathogenicity refers to pathogenic microorganisms in the cultivation system that are not pathogenic to the plant 100, although they have a longer duration of infection, a slower spread, and less pathogenicity than Class I pests. These include some broad-spectrum pathogens like *Botrytis cinerea* or nematodes, which infest vegetables such as melons, solanaceous fruits, and cabbage, but cause relatively minor damage. Fourth-level pathogenicity refers to the lack of pathogenicity of pathogenic microorganisms in the cultivation system on the plant 100. While Class III pests are indeed harmful, they do not cause disease on the plant 100 in the cultivation system. For example, some specialized pathogenic microorganisms, such as *Fusarium oxysporum* sesame-specific strains, are detected in the cultivation system for tomatoes, and therefore will not cause tomato diseases. Information related to beneficial microorganisms refers to information about beneficial microorganisms present in the aquatic environment where the roots of the plant 100 in the cultivation system are located, which are beneficial to the growth of the plant 100. This includes the types and concentrations of beneficial microorganisms, such as *Penicillium* and *Azotobacter*, which can inhibit pathogens. The information related to harmful microorganisms refers to the information contained in the aquatic environment where the roots of the plant 100 grown in the cultivation system are located, which is harmful to the growth of the plant 100, including: the types and concentrations of harmful microorganisms. The disinfection mode indicates the disinfection method, including disinfection concentration, disinfection frequency, and disinfection duration. The first threshold is preferably a critical value for the types and concentrations of harmful microorganisms with primary pathogenicity that cause significant diseases in the hydroponic plant or cause the death of the plant 100. The second threshold is preferably a critical value for the types and concentrations of harmful microorganisms with secondary pathogenicity that cause significant harm to the growth of the hydroponic plant 100. The third threshold is preferably a critical value for the types and concentrations of beneficial microorganisms that promote the growth of the hydroponic plant or are beneficial to the growth efficiency of the hydroponic plant.

[0062] Example 1

[0063] This embodiment uses a hydroponic tomato system as an example to illustrate the working process of the cultivation system, such as... Figure 1 As shown.

[0064] The plant cultivation system includes: a detection module configured to detect microbial-related information in a water sample 200 used for hydroponic plants 100 in the plant cultivation system, and a regulation unit configured to regulate the aquatic environment of the plant cultivation system based on the microbial-related information detected by the detection module, so as to promote the growth of beneficial microorganisms and inhibit the growth of harmful microorganisms.

[0065] The control unit 700 is configured to,

[0066] When information related to microorganisms indicates that the number and concentration of harmful microorganisms with primary pathogenicity in the water sample 200 of the hydroponic plant 100 exceed a first threshold, the water sample 200 of the cultivation system is disinfected in a first mode. Preferably, primary pathogenicity refers to a strong pathogenicity capable of rapidly causing disease or death in the plant 100 of the plant cultivation system. Preferably, the first mode refers to applying a disinfectant to the plant cultivation system at a concentration of 5-30 mg / L, with disinfection every 3-7 days for 10-20 minutes each time. Preferably, the first threshold is preferably a critical value for the number and concentration of harmful microorganisms with primary pathogenicity that cause significant disease or death in the hydroponic plant 100. Preferably, the control unit 700 is configured to disinfect the water sample 200 of the cultivation system in a second mode when information related to microorganisms indicates that the number and concentration of harmful microorganisms with primary pathogenicity in the water sample 200 of the hydroponic plant 100 are below the first threshold. Preferably, the second mode refers to applying disinfectant to the plant cultivation system at a concentration of 5-30 mg / L, with disinfection every 8-15 days for 10-20 minutes each time. Preferably, the control unit 700 is configured to, when information related to microorganisms indicates that the types and concentrations of harmful microorganisms with secondary pathogenicity in the water sample 200 of the hydroponic plant 100 exceed a second threshold, control the detection module to acquire information related to beneficial microorganisms. Wherein, when the types and concentrations of the beneficial microorganisms exceed a third threshold, the water sample 200 of the cultivation system is disinfected in a third mode. Preferably, the secondary pathogenicity refers to the pathogenicity that causes disease in the plant 100 of the plant cultivation system for a long period with low disease severity. Preferably, the third mode refers to applying disinfectant to the plant cultivation system at a concentration of 1-5 mg / L, with disinfection every 8-15 days for 2-10 minutes each time. The second threshold is preferably a critical value for the types and concentrations of harmful microorganisms with secondary pathogenicity that cause significant harm to the growth of the hydroponic plant 100. The third threshold is preferably a critical value for the types and concentrations of beneficial microorganisms that promote the growth of hydroponic plants 100 or are beneficial to the growth efficiency of hydroponic plants 100. Preferably, the control unit 700 is configured to, when the information related to microorganisms indicates that the types and concentrations of harmful microorganisms with secondary pathogenicity in the water sample 200 of the hydroponic plants 100 exceed the second threshold, control the detection module to acquire information related to beneficial microorganisms, wherein, when the types and concentrations of beneficial microorganisms are below the third threshold, the water sample 200 of the cultivation system is disinfected in a fourth mode. Preferably, the fourth mode refers to applying disinfectant to the plant cultivation system in a mode of disinfection every 8 to 15 days, each disinfection lasting 2 to 10 minutes, at a concentration of 5 to 30 mg / L.

[0067] The cultivation system includes: a collection module 300 configured to collect water samples 200 from the cultivation system; a detection module configured to detect signals related to microorganisms in the water sample 200; a disinfection control unit 710 configured to adjust the disinfection mode based on the analysis results of the control unit 700; and a control unit 700 capable of generating analysis results based on the microorganisms in the water sample 200, wherein the analysis results refer to:

[0068] Based on the signals collected by the detection module, the control unit 700 can control the disinfection control unit 710 to generate the corresponding disinfection mode according to the contribution of microorganisms in the water sample 200 to the plants.

[0069] According to a preferred embodiment, the detection module includes: a first detection unit 800 configured to detect information related to harmful organisms in the water sample 200; and a second detection unit 900 configured to detect information related to beneficial microorganisms in the water sample 200. The control unit 700 is equipped with a first threshold to analyze information related to harmful organisms, including first-class harmful organisms with primary pathogenicity to the plants 100 grown in the cultivation system, second-class harmful organisms with secondary pathogenicity, and third-class harmful organisms with tertiary pathogenicity.

[0070] The acquisition module 300 sends the collected water sample 200 to the first detection unit 800 for testing. The first detection unit 800 outputs information related to harmful organisms. Preferably, the first detection unit 800 is communicatively connected to the control unit 700 to send the acquired information to the control unit 700. The acquisition module 300 sends the collected water sample 200 to the second detection unit 900 for testing. The second detection unit 900 outputs information related to beneficial microorganisms. Preferably, the second detection unit 900 is communicatively connected to the control unit 700 to send the acquired information to the control unit 700. The control unit 700 is set with a first threshold and a second threshold to analyze information related to harmful microorganisms. The control unit 700 is set with a third threshold to analyze information related to beneficial microorganisms. Preferably, the first detection unit 800 can be a high-throughput sequencer, such as a T20 sequencer (model DNBSEQ-T20×2), a HiSeq 2500 sequencer, or a MiSeq sequencer. Preferably, in this embodiment, an ozone generator of model GY-CYFSQ-400g can be used for disinfection. Preferably, the disinfectant is of different types. Further, the disinfectant can be a bactericidal product compounded with hydrogen peroxide and silver ions, ozone, etc.

[0071] According to a preferred embodiment, when the plant 100 grown in the cultivation system is tomato, the first threshold is set to 2.4 × 10⁻⁶. 5 / mL. For example, when the concentration of Fusarium oxysporum tomato-specific spores exceeds the first threshold, the control unit 700 controls the disinfection control unit 710 to activate the first disinfection mode. The first disinfection mode is a round of disinfection every 3-7 days, with 3 consecutive disinfections per round, each disinfection time being 10-20 minutes, and the ozone water concentration being 7.3 mg / L. Preferably, the ozone concentration is 5 mg / L. Preferably, the ozone concentration is 30 mg / L. The reason for this setting is that when the type and concentration of the first type of harmful organisms exceed the first threshold, due to their rapid reproduction, large spore production, and strong spore dispersal ability, the primary purpose when this occurs is to quickly reduce the type and concentration of the first type of harmful organisms in the cultivation system. Otherwise, their spores will rapidly reproduce and germinate, and the degree of harm to the plant 100 will rapidly increase, and the plant 100 will face death. In addition, spores can float in the air and attach to a large area of ​​plant 100. Under suitable temperature conditions, the spore germination rate is fast, which may cause the death of a large area of ​​plant 100. The first disinfection mode is strong enough to quickly reduce the types and concentrations of pathogenic microorganisms.

[0072] When the number and concentration of the first category of harmful organisms detected are below a first threshold and greater than 0, for example, 2.4 × 10⁻⁶... 5 The control unit 700 controls the disinfection control unit 710 to activate the second disinfection mode. The second disinfection mode involves disinfection every 8-15 days, with three consecutive disinfections per cycle, each lasting 10-20 minutes, and an ozone concentration of 7.3 mg / L. Preferably, the ozone concentration is 5 mg / L. More preferably, the ozone concentration is 30 mg / L. This is because, although fungal pathogens reproduce rapidly, produce a large number of spores, and have strong spore dispersal capabilities, the types and concentrations of fungal pathogens in the culture water are relatively low at this stage. Disinfection using the second mode can eliminate fungal pathogens in the water during the first disinfection, achieving sterilization. Disinfecting the system at intervals reduces the impact of the disinfectant on the plants themselves.

[0073] According to a preferred embodiment, the control unit 700 is further provided with a second threshold for analyzing the first signal. When the detected harmful organism is a second type of harmful organism, the control unit 700 is configured to: control the disinfection control unit 710 not to activate disinfection when the second type of harmful organism is below the second threshold; and control the second detection unit 900 to activate to obtain information related to beneficial microorganisms when the second type of harmful organism is above the second threshold. The control unit 700 is also provided with a third threshold for analyzing information related to beneficial microorganisms.

[0074] According to a preferred embodiment, the second threshold is set to 10. 4 / mL. When the types and concentrations of the second category of harmful organisms are detected to be below the second threshold, the disinfection control unit 710 will not activate the disinfection mode regardless of the types and concentrations of beneficial microorganisms. The reason for this setting is that for pathogenic microorganisms with moderate pathogenicity, when their types and concentrations are low, their presence will not temporarily affect the growth of plant 100. If plant 100 is disinfected and sterilized under such circumstances, it will actually be detrimental to the growth of plant 100. For example, when the types and concentrations of bacterial pathogenic microorganisms are close to trace amounts, the types and concentrations of beneficial microorganisms in the water are high. When the system is disinfected and sterilized, although bacterial pathogenic microorganisms can be eliminated, the beneficial microorganisms to plant 100 growth will be lost. The types and concentrations of beneficial microorganisms can also decrease. Some beneficial microorganisms can stimulate the plant's immune system to induce resistance; others can produce endogenous enzymes and plant growth regulators to promote plant growth. For example, beneficial bacteria such as *Bacillus mucilaginosus*, *Bacillus laterosporus*, and *Bacillus licheniformis* can promote root growth, increase fibrous roots, promote leaf photosynthesis, regulate the flow of nutrients to fruits, and significantly increase fruit size and yield. Some beneficial microorganisms can transform poorly absorbed nutrients into forms that plants can utilize, such as nitrogen and phosphorus, to improve the efficiency of nutrient absorption by plant roots and accelerate plant growth. When the types and concentrations of pathogenic bacteria near the plant roots are low, they will not affect the normal growth of the plant. However, when the types and concentrations of beneficial microorganisms near the roots are high or low, disinfecting the system can actually reduce the types and content of beneficial microorganisms in the water, thereby reducing the plant's growth efficiency.

[0075] Preferably, the information related to harmful microorganisms can be the species and concentration of a first category of harmful organisms. Preferably, the information related to harmful microorganisms can be the species and concentration of a second category of harmful organisms.

[0076] When the first detection unit 800 detects that the type and concentration of the second type of harmful organisms exceed a second threshold, the control unit 700 controls the second detection unit 900 to activate, in order to obtain the type and concentration of beneficial microorganisms. The control unit 700 is equipped with a third threshold to determine the type and concentration of beneficial microorganisms. According to a preferred embodiment, the third threshold is for rhizobia and Bacillus subtilis, with a concentration of 10. 8 cfu / kg.

[0077] When the types and concentrations of second-category harmful organisms exceed the second threshold, and the types and concentrations of beneficial microorganisms exceed the third threshold, the control unit 700 controls the disinfection control unit 710 to activate the third disinfection mode. The third disinfection mode involves disinfection every 8-15 days, with two consecutive disinfections per cycle, each lasting 2-10 minutes, and an ozone water concentration of 1.7 mg / L. Preferably, the ozone concentration is 1 mg / L. More preferably, the ozone concentration is 5 mg / L. The purpose of this setting is as follows: Although the pathogenicity (weak reproductive capacity / slow germination) of the second type of pests is moderate, Plant 100 still faces the risk of death if its species and concentration exceed the second threshold. However, due to the high species and concentration of beneficial microorganisms in the rhizosphere, Plant 100 has a strong disease resistance in some cases. For example, some beneficial microorganisms can activate Plant 100's immune system to increase its disease resistance. In this case, it is necessary to take auxiliary measures of disinfection to control pathogenic microorganisms and also to ensure the survival of beneficial microorganisms. Under the condition of the presence of beneficial microorganisms, especially when the species and concentration of beneficial microorganisms are both high, they can play a role in disease resistance. Therefore, the disinfection mode is set to low frequency, short time, and low concentration to ensure that the species and concentration of beneficial microorganisms are at a high level to the maximum extent while controlling pathogenic microorganisms, so as to reduce the damage of disinfection to Plant 100 itself.

[0078] When the types and concentrations of the second category of harmful organisms exceed the second threshold, and the types and concentrations of beneficial microorganisms are below the third threshold, the control unit 700 controls the separate control unit to activate the fourth disinfection mode. The fourth disinfection mode involves disinfection every 8-15 days, with two consecutive disinfections per cycle, each lasting 2-10 minutes, and an ozone water concentration of 7.3 mg / L. Preferably, the ozone concentration is 5 mg / L. More preferably, the ozone concentration is 30 mg / L. The purpose of this setup is as follows: Although the pathogenicity (weak reproductive capacity / slow germination) of the second type of pests is moderate, if their species and concentration exceed the second threshold, Plant 100 is at risk of death. Although beneficial microorganisms also exist, their species and concentration are low. Even if beneficial microorganisms have the function of stimulating Plant 100's immune system, their limited species and concentration make them insufficient to resist the damage caused by the second type of pests, which are more numerous and concentrated. In this case, controlling the damage of the second type of pests to Plant 100's growth is the primary objective. Under the condition of achieving the primary objective, the disinfection mode with the least harm to beneficial microorganisms is selected, i.e., the fourth disinfection mode is selected. High concentrations of disinfectants have a better control effect on pathogenic microorganisms. The first high-concentration disinfection will have a better control effect on pathogenic microorganisms. Selecting low-frequency and short-time disinfection is to minimize the damage to beneficial microorganisms and Plant 100 itself.

[0079] Preferably, the acquisition module 300 periodically acquires water in the area near the roots of the plant 100 as the detection object of the first detection unit 800 and / or the second detection unit 900.

[0080] Preferably, the first detection unit 800 and the second detection unit 900 can be optical densitometers, which detect the OD value of the bacterial suspension to obtain the bacterial concentration. Preferably, the first detection unit 800 and the second detection unit 900 can be fully automated microbial quantitative analyzers (LD-ATP). Preferably, the second detection unit 900 can be a high-throughput sequencer, such as a T20 sequencer (model DNBSEQ-T20×2), a HiSeq 2500 sequencer, or a MiSeq sequencer. After sequencing, the species and concentration / abundance of pathogens are obtained.

[0081] When the detected harmful organism is classified as a third-category harmful organism, the disinfection control unit 710 is controlled not to activate disinfection. If pathogenic microorganisms such as *Fusarium oxysporum* sesame-specific type and *Fusarium oxysporum* wilt-specific type are detected in the tomato cultivation water, their presence will not affect the growth of the tomatoes, and disinfection is unnecessary. If disinfection and sterilization are performed on the tomatoes under such circumstances, the disinfectant will actually be detrimental to the growth of the tomatoes.

[0082] Example 2

[0083] The growth process of Plant 100 is affected by a variety of factors, such as light, temperature, humidity, oxygen, and gas circulation. Adjusting these factors can create suitable conditions for Plant 100 growth. Furthermore, Plant 100 is susceptible to pests and diseases under certain conditions. For example, under humid conditions and low light, Plant 100 is prone to gray mold. In addition to disinfecting the cultivation system, the environmental conditions of the cultivation system can be adjusted in a targeted manner to reduce the spread rate of pathogenic microorganisms. Therefore, the severity of Plant 100 diseases can be reduced, and the growth status of Plant 100 can be better controlled.

[0084] Preferably, the cultivation system further includes a temperature control unit 400 configured to control the ambient temperature of the cultivation system. Preferably, the temperature control unit 400 is communicatively connected to the control unit 700. The control unit 700 can adjust the temperature parameters of the temperature control unit 400 to change the ambient temperature of the cultivation system. For example, the control unit 700 sends a temperature command of 25°C to the temperature control unit 400. After receiving the command, the temperature control unit 400 sets the temperature parameter to 25°C and maintains the ambient temperature of the cultivation system at 25°C to provide a 25°C temperature condition for the plants 100 growing in the cultivation system.

[0085] Preferably, the cultivation system further includes an oxygen content control unit 500, which is configured to control the oxygen content of the environment of the cultivation system. Preferably, the oxygen content control unit 500 is communicatively connected to the regulation unit 700. The regulation unit 700 can adjust the oxygen content parameter of the oxygen content control unit 500 to change the oxygen content in the environment of the cultivation system. For example, the regulation unit 700 sends an oxygen content command of 7-8 mg / L or 15% to the oxygen content control unit 500. After receiving the command, the oxygen content control unit 500 sets the oxygen content parameter to 7-8 mg / L or 15% and maintains the oxygen content of the root environment of the cultivation system at 7-8 mg / L or 15% to provide suitable oxygen conditions for the plants 100 growing in the cultivation system.

[0086] Preferably, the cultivation system further includes a lighting control unit 600 configured to control the illumination of the cultivation system. Preferably, the lighting control unit 600 is communicatively connected to a control unit 700. The control unit 700 can adjust the illumination parameters of the lighting control unit 600 to change the ambient light of the cultivation system. Preferably, the illumination parameters can include a light cycle and a light intensity. For example, the control unit 700 sends a illumination command to the lighting control unit 600: a light cycle of 8 hours in darkness / 16 hours in light, and a light intensity of 2000 lux. After receiving the command, the lighting control unit 600 sets the illumination parameters to 8 hours in darkness / 16 hours in light, and 2000 lux, providing the plants 100 growing in the cultivation system with a light cycle of 8 hours in darkness / 16 hours in light and a light intensity of 2000 lux.

[0087] According to a preferred embodiment, the control unit 700 can adjust the environmental conditions of the cultivation system according to the type of pathogen infecting the plant 100. For example, when the plant 100 is infected with Botrytis cinerea, the control unit 700 determines that this type of pathogen is prone to growth under conditions of sufficient oxygen and weak light. Therefore, the control unit 700 controls the lighting control unit 600 and the oxygen content control unit 500 to adjust parameters. Without affecting the growth efficiency of the plant 100, the control unit 700 appropriately increases the light intensity of the lighting control unit 600, for example, increasing the light intensity from 1500 lux to 2000 lux; the control unit 700 controls the oxygen content control unit 500 to appropriately decrease the oxygen content without affecting plant growth, for example, decreasing the oxygen content from 15% to 10%. The oxygen content control unit 500, the temperature control unit 400, and the lighting control unit 600 directionally adjust the environmental conditions of the cultivation system to reduce the spread rate of pathogenic microorganisms.

[0088] It should be noted that the specific embodiments described above are exemplary. Those skilled in the art can devise various solutions inspired by the disclosure of this invention, and these solutions all fall within the scope of this invention and its protection. Those skilled in the art should understand that this specification and its accompanying drawings are illustrative and do not constitute a limitation on the claims. The scope of protection of this invention is defined by the claims and their equivalents. This specification contains multiple inventive concepts; terms such as "preferredly," "according to a preferred embodiment," or "optionally" indicate that the corresponding paragraph discloses an independent concept. The applicant reserves the right to file divisional applications based on each inventive concept. Throughout the text, features introduced by "preferredly" are merely optional and should not be construed as mandatory. Therefore, the applicant reserves the right to abandon or delete relevant preferred features at any time.

Claims

1. A plant cultivation system comprising root microorganisms, characterized in that, include: The detection module is configured to detect microbial-related information in a water sample (200) used for hydroponic plants in the plant cultivation system, and The control unit (700) is configured to adjust the aquatic environment of the plant cultivation system based on microbial-related information detected by the detection module, so as to promote the growth of beneficial microorganisms and inhibit the growth of harmful microorganisms; the control unit (700) is configured to, When the microbial-related information shows that the water sample (200) of the hydroponic plant has a number and concentration of harmful microorganisms with first-order pathogenicity exceeding a first threshold, the water sample (200) of the cultivation system is disinfected in a first mode. When the information related to microorganisms indicates that the water sample (200) of the hydroponic plant (100) contains harmful microorganisms with secondary pathogenicity exceeding a second threshold, the control detection module acquires information related to beneficial microorganisms, wherein... When the types and concentrations of the beneficial microorganisms exceed the third threshold, the water sample (200) of the cultivation system is disinfected in the third mode.

2. The system according to claim 1, characterized in that, The primary pathogenicity refers to the strong pathogenicity that can rapidly cause disease or death in the plants (100) of the plant cultivation system. The first mode refers to applying disinfectant to the plant cultivation system at a concentration of 5-30 mg / L, with each disinfection cycle lasting 10-20 minutes every 3-7 days. The first threshold is a critical value for the types and concentrations of harmful microorganisms with first-order pathogenicity that cause major diseases or plant death in the hydroponic plant (100).

3. The system according to claim 2, characterized in that, The control unit (700) is configured to, When the microbial-related information shows that the water sample (200) of the hydroponic plant (100) has a type and concentration of harmful microorganisms with first-order pathogenicity below the first threshold, the water sample (200) of the cultivation system is disinfected in the second mode.

4. The system according to claim 3, characterized in that, The second mode refers to applying disinfectant to the plant cultivation system in a manner that involves disinfection every 8 to 15 days, each disinfection lasting 10 to 20 minutes, at a concentration of 5 to 30 mg / L.

5. The system according to claim 1, characterized in that, The secondary pathogenicity refers to the pathogenicity that causes diseases in the plants (100) of the plant cultivation system to occur over a long period of time with low disease severity. The third mode refers to applying disinfectant to the plant cultivation system every 8-15 days, for 2-10 minutes each time, at a concentration of 1-5 mg / L. The second threshold is a critical value for the types and concentrations of harmful microorganisms with secondary pathogenicity that pose a significant threat to the growth of the hydroponic plant (100). The third threshold is a critical value for the types and concentrations of beneficial microorganisms that promote the growth of the hydroponic plant (100) or are conducive to the growth efficiency of the hydroponic plant (100).

6. The system according to claim 5, characterized in that, The control unit (700) is configured to, When the information related to microorganisms indicates that the water sample (200) of the hydroponic plant (100) contains harmful microorganisms with secondary pathogenicity exceeding a second threshold, the control detection module acquires information related to beneficial microorganisms, wherein... When the types and concentrations of the beneficial microorganisms are below the third threshold, the water sample (200) of the cultivation system is disinfected in the fourth mode.

7. The system according to claim 6, characterized in that, The fourth mode refers to applying disinfectant to the plant cultivation system in a mode of disinfection every 8 to 15 days, each disinfection lasting 2 to 10 minutes, with a concentration of 5 to 30 mg / L.

8. A method for using a plant cultivation system comprising root microorganisms as described in any one of claims 1 to 7, characterized in that, Includes the following steps: Obtain water samples (200) from the cultivation system; Obtain information related to microorganisms from the water sample (200); The aquatic environment of the plant cultivation system is adjusted based on information related to microorganisms.

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