Hnrnpa3 gene affecting replication of pedv and application thereof

CN116694636BActive Publication Date: 2026-10-16NORTHWEST A & F UNIV
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Patent Information

Application Number
CN202310647715.5
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-06-01
Publication Date
2026-10-16
Estimated Expiration
2043-06-01

AI Technical Summary

Technical Problem

但HNRNPA3在PEDV感染过程中的作用完全未知

Benefits of technology

[0018] As can be seen from the above technical solution, compared with the prior art, this invention discloses an HNRNPA3 gene that can affect PEDV replication. It utilizes siRNA targeting HNRNPA3 to specifically knock down HNRNPA3 and specifically overexpress HNRNPA3, and then uses RT-qPCR and TCID50 to... 50 The effect of HNRNPA3 on PEDV replication was detected by Western blot. Specific knockdown of HNRNPA3 promoted PEDV replication, while specific overexpression of HNRNPA3 inhibited PEDV replication. The porcine HNRNPA3 gene holds promise for applications in the development of drugs, vaccines, and genetically modified pigs resistant to porcine epidemic diarrhea (PED), providing new methods and perspectives for the effective control of PED.

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Abstract

The application discloses an HNRNPA3 gene capable of affecting PEDV replication, and utilizes a eukaryotic expression vector to mediate specific high expression of HNRNPA3 and specific knockdown of HNRNPA3 by targeting siRNA of HNRNPA3, then the influence of HNRNPA3 on PEDV replication is detected by RT-qPCR, TCID 50 and western blot. The high expression of HNRNPA3 has an inhibiting effect on PEDV replication, the knockdown of HNRNPA3 has an effect of promoting PEDV replication, and the HNRNPA3 gene is expected to be applied to development of medicines, vaccines and gene modified pigs for preventing or treating porcine epidemic diarrhea, and provides a new method and a brand new perspective for effective prevention and control of the porcine epidemic diarrhea.
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