A soybean enzymatic polypeptide and its application as an elastase inhibitor
By screening out the short peptides FADCYSDS and NVDSGFE with elastase inhibitory activity from the hydrolyzed polypeptide of soy protein, soybean enzymatic polypeptide with significant inhibitory activity was prepared, which solved the shortcomings of elastase inhibitory peptides in the prior art in terms of inhibitory activity, and achieved effective treatment for inflammatory diseases such as chronic bronchitis, pulmonary cystic fibrosis and rheumatoid arthritis.
Patent Information
- Application Number
- CN202311240520.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2023-09-25
- Publication Date
- 2025-04-04
- Estimated Expiration
- 2043-09-25
AI Technical Summary
The existing elastase inhibitory peptides still have room for improvement in inhibiting the activity of elastase, and it is difficult to meet the needs of fighting inflammatory diseases such as chronic bronchitis, pulmonary cystic fibrosis and rheumatoid arthritis.
Short peptides with elastase inhibitory activity were screened from the hydrolyzed polypeptides of soy protein, with specific amino acid sequences being FADCYSDS and/or NVDSGFE, and polypeptides with inhibitory activity were prepared by alkaline and acid protease hydrolysis and solid phase synthesis.
The prepared soybean enzymatic polypeptide significantly inhibits the activity of elastase, has a low semi-inhibitory concentration (IC50 value is 0.325±0.012 mg/mL and 0.346±0.021 mg/mL), and is suitable for the treatment of a variety of inflammatory diseases while being economically beneficial.