Application of lipid droplet-targeted AIE molecule in seaweed staining imaging and screening

By using AIE molecules with aldehyde groups replacing the triphenylamine backbone for specific staining of algal lipid droplets, and combining this with flow cytometry screening technology, the problem of low yield of algal biocrude oil in existing technologies has been solved. This has enabled clear observation and efficient screening of algal lipid droplets, thereby increasing the yield of algal biocrude lipids.

CN117074374BActive Publication Date: 2026-04-17HKUST SHENZHEN RES INST
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Patent Information

Application Number
CN202210507400.6
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-05-10
Publication Date
2026-04-17
Estimated Expiration
2042-05-10

AI Technical Summary

Technical Problem

In existing technologies, the use of commercial probe Nile Red to stain seaweed has poor stability and low sensitivity, making it difficult to effectively screen out high-performing seaweed, resulting in low biocrude oil production.

Method used

Aggregation-induced emission organic small molecule materials (AIE molecules) with aldehyde-substituted triphenylamine backbones were used to specifically stain lipid droplets of seaweed, and combined with flow cytometry screening technology, to achieve clear observation and screening of seaweed lipid droplets.

Benefits of technology

It improves the clarity of observation and screening efficiency of lipid droplets in seaweed, increases the yield of lipids in seaweed by about two times, and has good biocompatibility and lipid droplet targeting.

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Abstract

The present application relates to a kind of lipid droplet targeted AIE molecule in seaweed dyeing imaging and screening etc., the AIE molecule is a kind of aldehyde group substitution triphenylamine skeleton aggregation-induced emission organic fluorescent molecule, it has bright blue-green fluorescence, good biocompatibility, excellent seaweed lipid droplet targeting nature.Compared with commercial probe Nile red, it has better specific marking effect. After using this probe to dye seaweed, the size, content and change of lipid droplet in seaweed can be clearly observed.Combined with flow cytometry screening technology, seaweed with strong fluorescent signal is screened, and seaweed with more and larger lipid droplets is obtained, and the culture result shows that the yield of seaweed biological lipids and the proportion of lipid material in dry weight are significantly improved, about twice of the original culture before screening.Therefore, it has important significance and value in marine microalgae research and biological crude oil production etc.
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