A skin care composition with lightening and whitening effects

Abstract

This invention discloses a skincare composition with brightening and whitening effects. The skincare composition of this invention is a compound of sodium hyaluronate-ferulic acid graft, *Salvia splendens* extract, and / or peony root extract. Experiments have shown that it has excellent brightening and whitening effects and can be used in cosmetics to improve dull skin tone and darkened skin.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to a skincare composition with brightening and whitening effects. Background Technology

[0002] Skin tone differences are primarily related to the amount of melanin in the skin. "Skin whitening" has always been a constant theme in the beauty market, with consistently huge market demand. Whitening, fading spots, removing blemishes, brightening, and darkening are the main demands of beauty whitening functions. The depth of skin color is regulated by multiple factors, mainly depending on the content and distribution of melanin. Melanin is an amino acid derivative, formed from tyrosine or 3,4-dihydroxyphenylalanine through a series of chemical reactions, and it often exists in a polymerized form. Melanin is synthesized by melanocytes in the basal layer of the human epidermis. When ultraviolet rays irradiate the skin, the skin enters a "self-protective" state, activating tyrosinase to catalyze the oxidation of tyrosine or 3,4-dihydroxyphenylalanine to produce melanin to protect skin cells. Melanin then moves through layers of cell metabolism, reaching the epidermis to form freckles, sunspots, and dark spots.

[0003] Currently used whitening agents such as 4-butylresorcinol, kojic acid, and vitamin C, while having significant whitening and brightening effects, can irritate the skin. People with sensitive and fragile skin may not tolerate them. Long-term use of these irritating ingredients can cause inflammation, which can exacerbate melanin production, thus preventing products containing these whitening ingredients from achieving the expected results.

[0004] To improve dull skin tone, it's not only necessary to find and develop more effective ingredients, but also to research how to apply these effective ingredients to cosmetics to truly improve the skin health of the general public. At the same time, the products themselves should be highly safe and have low irritation.

[0005] Sodium hyaluronate-ferulic acid grafts are already used in skincare product formulations. The applicant previously developed an anti-photoaging skincare composition and its preparation method and application (CN202311268636X). This composition is a combination of sodium hyaluronate-ferulic acid grafts and one or two of the following: Viola yedoensis flower extract and Calendula officinalis flower extract. Experimental verification showed that it can achieve anti-photoaging effects by promoting the formation of elastic fibers and Collagen IV. Subsequently, the applicant developed a new enzymatic method for preparing sodium hyaluronate-ferulic acid grafts (CN2024100507594). The product prepared by this method has good water solubility and high stability, showing great application potential in the cosmetics field. Building on this, the applicant continues to develop new functions of sodium hyaluronate-ferulic acid grafts in hopes of achieving more applications. Summary of the Invention

[0006] To address the aforementioned problems, this invention provides a skincare composition with brightening and whitening effects. The composition is formulated from sodium hyaluronate-ferulic acid graft, *Salvia miltiorrhiza* extract, and / or peony root extract. Experiments have demonstrated that it possesses excellent brightening and whitening effects and can be used in cosmetics to improve dull skin tone and darkening.

[0007] The technical solution of the present invention is: a skin care composition with brightening and whitening effects, comprising the following components in parts by weight: 0.1 to 1.5 parts of sodium hyaluronate-ferulic acid graft, and 1.0 to 30.0 parts of Salicornia glutinosa extract and / or Paeonia lactiflora root extract.

[0008] Preferred proportions: 0.3-1.0 parts of sodium hyaluronate-ferulic acid graft, 1.0-10.0 parts of Salicornia glutinosa extract, and 1.0-10.0 parts of Paeonia lactiflora root extract.

[0009] The sodium hyaluronate-ferulic acid graft used in this invention can be obtained by grafting sodium hyaluronate and ferulic acid by chemical method (CN202311268636X) or by biological enzymatic method (CN2024100507594), preferably by biological enzymatic method.

[0010] The *Salvia splendens* extract can be obtained commercially. A preferred method is as follows: Add dried *Salvia splendens* powder to a 60%–80% ethanol solution, extract 2–4 times in a water bath at 70–80°C, combine the filtrates, concentrate under reduced pressure, and volatilize until no alcohol odor remains. Add 0.8–1.2 times the mass of butanediol, stir well, and filter to obtain the *Salvia splendens* extract. The concentration, based on crude drug, is 0.40–0.55 g / mL, meaning 0.40–0.55 g of dried *Salvia splendens* powder yields 1 mL of *Salvia splendens* extract (solution).

[0011] The peony root extract can be obtained commercially. A preferred method is as follows: Pulverize dried peony roots, add a 30%–50% ethanol solution, and extract by reflux at 75–90°C 2–4 times. Combine the filtrates. Concentrate the filtrate under reduced pressure, evaporate until no alcohol odor remains, add 1.5–2.0 times the weight of the concentrate of glycerol, stir well, and filter to obtain the peony root extract. The concentration, based on the crude drug, is 0.25–0.40 g / mL, meaning 0.25–0.40 g of dried peony root powder yields 1 mL of peony root extract (solution).

[0012] Preparation method: Sodium hyaluronate-ferulic acid graft, Salicornia glutinosa extract, and Paeonia lactiflora root extract are mixed together and dissolved in purified water (to make up to 100 parts).

[0013] This invention also provides the application of the above-described composition in the preparation of brightening and whitening skincare cosmetics. Through experimental verification, this invention demonstrates that the above-described composition can effectively brighten and whiten the skin by inhibiting melanin synthesis, while being safe and free of toxic side effects; therefore, it can be applied to brightening and whitening skincare cosmetics.

[0014] The present invention also provides a brightening and whitening skin care cosmetic, characterized in that the cosmetic contains 0.5-30% (preferably 2-20%) of a brightening and whitening skin care composition.

[0015] The aforementioned brightening and whitening skincare cosmetics may also contain emulsifiers, emollients, moisturizers, thickeners, preservatives, pH adjusters, and other raw materials permitted in the cosmetics industry.

[0016] Furthermore, by rationally adding the above-mentioned raw material components, this invention can also prepare different cosmetic formulations, such as essence water, essence liquid, facial mask, essence lotion, essence cream, etc. In addition, based on the above-mentioned basic cosmetic categories, further derivative cosmetic categories can be obtained, which obviously all fall within the protection scope of this application.

[0017] The beneficial technical effects of this invention are:

[0018] 1. In vitro zebrafish embryo melanin production inhibition assays demonstrate that the sodium hyaluronate-ferulic acid graft of this invention has a synergistic effect with one or two of the extracts of *Salvia miltiorrhiza* and *Paeonia lactiflora* root extract, achieving a brightening and whitening effect by inhibiting melanin production. Compared to the negative control, cosmetics containing the skincare composition of this invention showed significant improvements in ITA° difference, MI difference, and visual skin tone grade difference at 1, 2, 3, and 4 weeks after use, indicating that the product has whitening efficacy.

[0019] 2. The preparation method of the brightening and whitening skin care cosmetic of the present invention is simple and easy to implement, and is suitable for mass production, thus having good practical application value. Attached Figure Description

[0020] Figure 1 The results of the inhibition rate of melanin production in zebrafish by different test samples are shown. ** indicates that p < 0.01 compared with the blank control. Detailed Implementation

[0021] The present invention will be further illustrated below by means of embodiments, but these embodiments are not intended to limit the invention to their scope. Based on the embodiments of the present invention, any modifications to the present invention made by those skilled in the art without inventive effort are within the scope of protection of the present invention. Furthermore, in the embodiments of the present invention, unless otherwise specified, all raw materials used in the preparation are commercially available products well-known to those skilled in the art.

[0022] Examples 1-5:

[0023] A brightening and whitening skincare composition comprises sodium hyaluronate-ferulic acid graft, *Salvia splendens* extract, and peony root extract. The formulations of Examples 1-5 and Comparative Examples 1-4 are shown in Table 1 below, calculated by mass ratio.

[0024] Preparation method: Sodium hyaluronate-ferulic acid graft, Salicornia glutinosa extract, and Paeonia lactiflora root extract (according to the ingredients and dosages in Table 1) are mixed together and dissolved in purified water.

[0025] Table 1: Component list (mass ratio) of Examples 1-5 and Comparative Examples 1-4

[0026]

[0027] The preparation method of the sodium hyaluronate-ferulic acid graft is as follows:

[0028] S1: Dissolve 2 kDa of sodium hyaluronate in water to obtain a sodium hyaluronate mass concentration of 0.1%, add 0.1% ethanol, and stir for 0.5 h;

[0029] S2: Add sodium carbonate to a final concentration of 0.1%, stir and react for 4 hours, then adjust the pH to 5.5 after the reaction is complete;

[0030] S3: Add ferulic acid to the above solution to make the ferulic acid mass concentration 0.02%; then add laccase (laccase activity 120 U / g) to the solution, the amount of enzyme added is 0.5% of the system mass, and react at 35℃ for 12 h;

[0031] S4: Collect the sample, filter and purify it using a 300D filter membrane, and freeze-dry the collected solution to obtain the final product.

[0032] The preparation method of *Salvia miltiorrhiza* extract is as follows: Take an appropriate amount of dried *Salvia miltiorrhiza* powder, add 70% ethanol aqueous solution at a mass ratio of 1:10, and extract in a water bath at 70-80℃ for 3 hours. Repeat the extraction once, combine the filtrates, concentrate the filtrate under reduced pressure, volatilize until no alcohol odor remains, add an equal mass of butanediol to the concentrate, stir evenly, and filter to obtain *Salvia miltiorrhiza* extract. The concentration is 0.45 g / mL based on crude drug, meaning 0.45 g of dried *Salvia miltiorrhiza* powder yields 1 mL of *Salvia miltiorrhiza* extract (solution).

[0033] The method for obtaining peony root extract is as follows: Dry peony root is pulverized and passed through a 150-mesh sieve. An appropriate amount of dried peony root powder is placed in a reflux jar, and 40% ethanol solution is added at a mass ratio of 1:8. The mixture is heated under reflux at 75–90°C for 1 hour, and the extraction is repeated twice. The filtrates are combined, concentrated under reduced pressure, and evaporated until no alcohol odor remains. Glycerin, at a mass ratio of 1.8 times that of the concentrate, is added, stirred evenly, and filtered to obtain the peony root extract. The concentration, based on the crude drug, is 0.35 g / mL, meaning 0.35 g of dried peony root powder yields 1 mL of peony root extract (solution).

[0034] Example 6: Essence Water

[0035] The above-mentioned brightening and whitening skincare composition is used to prepare an essence water product containing the brightening and whitening composition, and the formula is shown in Table 2.

[0036] Table 2: Formula of Essence Water Products

[0037]

[0038] Preparation method of essence water:

[0039] S1: Aqueous phase preparation: Add the raw materials from phase A separately, heat to 85℃ and stir to dissolve;

[0040] S2: When the temperature drops to 45℃, add phase B and phase C components in sequence, stir to dissolve and disperse evenly, and the essence water product is obtained.

[0041] The preparation method of Comparative Example 5 is the same as that of Example 6, except that Comparative Example 5 does not contain the brightening and whitening skin care composition, and the amount of brightening and whitening composition used in Example 6 is made up by adding water.

[0042] Example 7: Essence Lotion

[0043] The above-mentioned brightening and whitening skincare composition is used to prepare an essence product containing this anti-photoaging skincare composition, and the formula is shown in Table 3.

[0044] Table 3: Formulas of Essence Lotions

[0045]

[0046] Preparation method of essence lotion:

[0047] S1: Preparation of the aqueous phase: Add the raw materials from phase A separately, heat to 85℃ and stir to dissolve;

[0048] S2: Preparation of the oil phase: Add the raw materials from phase B separately and heat to 80℃ to melt;

[0049] S3: Combine phase A and phase B, turn on the homogenizer (3000 rpm), and homogenize for 12 min;

[0050] S4: After homogenization, add phase C, stir well, and start cooling;

[0051] S5: When the temperature drops to 70℃, add phase D, stir to dissolve, and continue to cool down after dissolution is complete;

[0052] S6: When the temperature drops to 45℃, add phase E, stir to dissolve and disperse evenly to obtain the essence lotion product.

[0053] Example 8: Essence Cream

[0054] The above-mentioned brightening and whitening skincare composition is used to prepare an essence cream product containing the brightening and whitening skincare composition, and the formula is shown in Table 4.

[0055] Table 4: Formula of Essence Cream Products

[0056]

[0057] Preparation method of essence cream:

[0058] S1: Preparation of the aqueous phase: Add the raw materials from phase A separately, heat to 85℃ and stir to dissolve;

[0059] S2: Preparation of the oil phase: Add the raw materials from phase B separately and heat to 80℃ to melt;

[0060] S3: Combine phase A and phase B, start homogenization (3000 r / min), and homogenize for 12 min;

[0061] S4: After homogenization is complete, start cooling. When the temperature drops to 70℃, add phase C, stir to dissolve, and continue cooling after dissolution is complete.

[0062] S5: When the temperature drops to 45℃, add phase D components, stir to dissolve and disperse evenly to obtain the essence cream product.

[0063] Experiment 1: Safety Testing Experiment

[0064] In accordance with SN / T 2329-2009 "Eye Irritation of Cosmetics / Chicken Embryo Villi Allantoic Membrane Test", the product was subjected to an eye irritation test, which is also known as a safety test.

[0065] Experimental method: Fertilized chicken embryos under 7 days old were incubated for 9 days in an incubator at 37.6±0.5℃ and 50%~70% humidity.

[0066] CAM preparation: Candling is performed to check the egg. The location of the air cell is marked on the eggshell surface. The marked portion of the eggshell is peeled off with tweezers to expose the white egg membrane. Care should be taken not to damage the integrity of the egg membrane. 0.5 mL of 0.9% NaCl solution is added to fully moisten the egg membrane. The surface liquid is gently absorbed with a paper towel, and the inner membrane is carefully removed with tweezers, ensuring that the vascular membrane is not damaged.

[0067] Reaction endpoint method: Take 0.3 mL of transparent test substance and add it evenly or apply it to the CAM surface. After 3 min of action, wash off the test substance with 0.9% NaCl solution and observe the degree of change of each toxic effect of CAM.

[0068] Endpoint score (ES): For tests using the reaction endpoint method, an endpoint score (ES) should be calculated, with the result rounded to two decimal places. The score per chicken embryo = the sum of observed hemorrhage, coagulation, and vascularization in each embryo; ES = the mathematical sum of the scores of all six embryos. Based on the ES values, classify the eye irritation of the test substance according to Table 5.

[0069] Table 5: Criteria for Judging Results of Reaction Endpoint Method

[0070] Irritant Classification Non-irritating / mildly irritating moderate irritation Strongly irritating / corrosive

[0071] The test results are shown in Table 6. The chicken embryo chorionic allantoic membrane test of the brightening and whitening skin care composition of Examples 1-5 showed no irritation, indicating that the combination of sodium hyaluronate-ferulic acid graft with one or two of the following: Salicornia glutinosa extract and Paeonia lactiflora root extract is safe, non-toxic, and has low irritation.

[0072] Table 6: Statistical Table of Stimulation Test Results

[0073]

[0074] Experimental Example 2: In vitro zebrafish embryo melanin production inhibition experiment

[0075] This experiment adopted T / SHRH036-2021 "Melanin Inhibition Test in Cosmetics - Zebrafish Embryo Test Method".

[0076] Experimental Principle: Human melanin is produced by the oxidation of tyrosine to dopa by tyrosinase. Dopa then undergoes oxidation and decarboxylation to become indolequinone, which finally polymerizes into melanin. The pigment formation-related genes in zebrafish are highly similar to those in humans in terms of sequence and regulatory mechanisms. Zebrafish embryos are small and transparent, facilitating microscopic imaging. Software is used to analyze and quantitatively read melanin levels, comparing changes in melanin levels in treated and control groups, and calculating the melanin inhibition rate to evaluate the melanin-inhibiting efficacy of the raw materials or products.

[0077] Experimental procedure:

[0078] Healthy zebrafish embryos, 6-8 hours post-fertilization, were used. Twenty embryos were randomly selected and transferred to 96-well plates, each containing one embryo and 0.2 mL of embryo culture medium (prepared by dissolving 2940 mg anhydrous calcium chloride, 1233 mg magnesium sulfate heptahydrate, 630 mg sodium bicarbonate, and 55 mg potassium chloride in 10 L of water, pH 6.5–8.5, all chemicals being analytical grade) as a blank control group. Twenty embryos were also randomly selected and transferred to 96-well plates, each containing one embryo and phenylthiourea working solution (prepared by dissolving 15 mg phenylthiourea in 10 mL of water) as a positive control group. Finally, twenty embryos were randomly selected and transferred to 96-well plates, each containing one embryo and 0.2 mL of the test solution (prepared by dissolving Examples 1-5 and Comparative Examples 1-3 in embryo culture medium to a 3 g / L test solution). Incubate in a constant temperature incubator at 28±1℃ for 48±1 hours after fertilization.

[0079] At least 12 fish embryos were randomly selected for each test group. Under a microscope, the unhatched embryos were carefully peeled off using tweezers, taking care not to damage the fish. The embryos were covered with 3% methylcellulose and placed back-up. They were then photographed under a stereomicroscope. All embryos were photographed using standardized parameters.

[0080] Open the photo using the analysis software ImageJ, mark the head and yolk dorsal area of ​​each fish embryo, then select "Measure Average Intensity" in the "Measure" column, and select the "Average Signal Intensity" from the test results as the melanin content index.

[0081] Calculate the melanin inhibition rate:

[0082] Inhibition rate = (CT) / (CP) × 100% ------------(1)

[0083] In the formula:

[0084] T—The average signal intensity of the fish embryos in the test substance treatment group;

[0085] C—The average signal intensity of fish embryos in the blank control group;

[0086] P—The average signal intensity of the fish embryos in the positive control group.

[0087] A two-tailed T-test was performed on the melanin signal intensity of fish embryos in the test group and the blank control group to obtain the p-value.

[0088] Depend on Figure 1It can be seen that, using phenylthiourea as a positive control, with a melanin inhibition rate of 100% for the positive control, the melanin inhibition rate of zebrafish embryos in Example 2 was 65%, in Example 1 it was 25%, in Example 3 it was 58%, in Example 4 it was 31%, in Example 5 it was 35%, in Comparative Example 1 it was 12%, in Comparative Example 2 it was 7%, in Comparative Example 3 it was 8%, and in Comparative Example 4 it was 10%, all showing inhibitory effects, with Example 2 showing the most significant inhibitory effect. A comparison of Examples 1-5 with Comparative Examples 1-4 revealed that the sodium hyaluronate-ferulic acid graft compound had a synergistic effect with one or both of the *Salvia miltiorrhiza* extract and *Paeonia lactiflora* root extract, while the *Salvia miltiorrhiza* extract or *Paeonia lactiflora* root extract alone had a lower inhibitory effect on melanin production. This also indirectly demonstrates that the components of the hyaluronic acid-ferulic acid graft compound, *Salvia miltiorrhiza* extract, and *Paeonia lactiflora* root extract have a synergistic effect.

[0089] The above results indicate that brightening and whitening compositions and cosmetics containing brightening and whitening compositions can effectively brighten and whiten the skin by inhibiting melanin production.

[0090] Test Example 3: Test Results of the Whitening Effect of Cosmetics Containing the Skin Care Composition of the Present Invention on Human Body

[0091] The whitening effect on human skin was evaluated based on the essence water product in Example 6.

[0092] The test plan is shown in Table 7.

[0093] Table 7: Basic Test Information Table

[0094]

[0095] Among them, the positive control sample, 7% VC product, was prepared according to the formula in Appendix I of the first method of the test method for the whitening and freckle-removing effects of cosmetics.

[0096] Test substance application: The testing personnel shall apply the test substance to the corresponding test area according to Table 7, twice a day, with an interval of no less than 4 hours between applications, and the application area shall be 7 cm². 2 The sample application rate was 2.00 ± 0.05 mg / cm³. 2 The interval between each test area should be no less than 1.0 cm.

[0097] 1. Testing instruments:

[0098] Sunlight Simulator (Model: UV Solar Simulator Model 601-300V2.5, Manufacturer: Solarlight Inc., USA).

[0099] Skin color testing probe (Model: Colorimeter CL400, Manufacturer: Courage and Khazaka GmbH, Germany).

[0100] Skin melanin and red pigment detection probe (model: Mexameter MX18, manufacturer: Courage and Khazaka GmbH, Germany).

[0101] 2. Indicator Definition

[0102] Minimum erythema dose (MED): The lowest dose of ultraviolet radiation (J / m²) required to cause a clearly visible erythema on the skin, covering most of the irradiated area. 2 () or the shortest time (s).

[0103] Individual type angle (ITA°): A parameter characterizing human skin color by measuring skin L*a*b* color space data using a skin colorimeter or reflectance spectrophotometer. The calculation formula (1) is as follows:

[0104]

[0105] Melanin index (MI): A parameter that characterizes the melanin content in the skin by measuring the absorption of a specific wavelength of light on the skin surface.

[0106] 3. Environmental conditions

[0107] The visual assessment and instrument testing were conducted in an environment with a temperature of 21±1℃ and a relative humidity of 50±10% RH. The visual assessment was conducted under constant lighting conditions (LED light with a color temperature of 5500~6500K). The subjects were assessed and tested after adapting to this environment for 30 minutes.

[0108] 4. Test Procedure

[0109] (1) Recruit volunteer subjects according to the requirements and obtain written informed consent. Before enrollment, ask subjects a series of questions about their medical history and health status according to the inclusion and exclusion criteria. At the same time, conduct conformity assessment and skin color test screening on the test site and record the results.

[0110] (2) Qualified subjects enter the stage of establishing a human skin melanization model. First, the MED of each subject's test site should be determined. Then, each test area is selected at the test site, and a sunlight simulator is used to irradiate the same irradiation point once a day at a dose of 0.75 times the MED, for 4 consecutive days.

[0111] (3) The skin darkening period is 4 days after the irradiation ends, and no treatment is required.

[0112] (4) On the 5th day after irradiation, visual evaluation and skin color instrument testing should be performed on the skin color of each test area. Test areas with poor consistency (areas where the ITA° value differs from the average value of all test areas by more than 5) should be removed. On the same day, the corresponding test substance should be applied to each blackened test area according to the random table.

[0113] (5) Apply the test substance continuously for 4 weeks. At 1, 2, 3 and 4 weeks after application, perform visual assessment and instrument detection on the skin color and record the results.

[0114] Skin colorimeter measurement: At each visit time, the L*, a*, and b* values ​​of each test area are measured using a skin colorimeter. Each area is tested three times, and the ITA° value is recorded and calculated. The larger the ITA° value, the lighter the skin color, and vice versa.

[0115] Skin melanin measurement: At each visit time, the MI value of each test area was measured with a skin melanin analyzer. Each test area was tested three times and the results were recorded. The smaller the MI value, the lower the skin melanin content, and vice versa.

[0116] 5. Data Analysis

[0117] Statistical analysis software was used to perform statistical analysis on the data. Quantitative data were expressed as mean ± standard deviation and tested for normality. If the data met the requirements of normality, paired t-tests were used for comparisons of the data before and after the same period; otherwise, rank-sum tests of two related samples were used. Ordinal data were compared before and after the same period using rank-sum tests of two related samples. Comparisons between the test and control areas were performed using independent samples t-tests or rank-sum tests. Simultaneously, the regression coefficients (slope k-values) of each parameter over time were calculated, with a significance level of P < 0.05 for all parameters.

[0118] 6. Record of abnormal phenomena and skin reactions during the test

[0119] Referring to the skin reaction grading standards for human trials stipulated in the "Cosmetic Safety Technical Specifications" (2015 edition), 31 volunteers participated in this trial, with 30 valid participants. No adverse reactions were observed in the test product area, negative control area, and positive control area of ​​the 30 volunteers who completed the trial, as shown in Table 8.

[0120] Table 8: Skin Reaction Record Sheet

[0121]

[0122] 7. Test Results

[0123] The test results for ITA°, MI, and visual skin tone rating are shown in Table 9-11.

[0124] Table 9: Comparison of test product, negative control, and positive control results before and after use

[0125]

[0126]

[0127] As can be seen from Table 9, except for the negative control, which showed no significant difference in ITA° and visual skin color grade after 1 week of use, the comparisons before and after use of the test product, negative control, and positive control all showed significant differences (because artificial sunspots will heal on their own over time, even without the product; however, the use of the test sample resulted in faster recovery compared to the control group).

[0128] Table 10: Comparison of the differences between the test products before and after use at different time points and the negative control.

[0129]

[0130] Table 11 shows the comparison of regression coefficients (slope k-value) of the test products with the negative control at any time point.

[0131]

[0132] The results showed that, compared with the negative control area, the ITA° difference of the test product was significantly improved at 1, 2, 3, and 4 weeks after use (P<0.05); the MI difference of the test product was significantly improved at 1, 2, 3, and 4 weeks after use (P<0.05); and the visual skin tone grade difference of the test product was significantly improved at 1, 2, 3, and 4 weeks after use (P<0.05). Regression coefficient analysis indicated that the test product significantly improved skin darkening compared with the negative control (P<0.05). This suggests that the product has a certain skin whitening effect.

[0133] It should be noted that the above examples are only used to illustrate the technical solutions of the present invention and not to limit it. Although the present invention has been described in detail with reference to the given examples, those skilled in the art can modify or make equivalent substitutions to the technical solutions of the present invention as needed, without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. A skincare composition with brightening and whitening effects, characterized in that, The product contains the following components in parts by weight: 0.1 to 1.5 parts of sodium hyaluronate-ferulic acid graft, and 1.0 to 30.0 parts of Salicornia glutinosa extract and / or Paeonia lactiflora root extract.

2. The skin care composition of claim 1 wherein, The product contains the following components in parts by weight: 0.3 to 1.0 parts of sodium hyaluronate-ferulic acid graft, 1.0 to 10.0 parts of Salicornia glutinosa extract, and 1.0 to 10.0 parts of Paeonia lactiflora root extract.

3. The skin care composition of Claim 1 or 2 wherein the composition is a skin care composition comprising a sunscreen agent. The preparation method of the *Salvia splendens* extract is as follows: take dried *Salvia splendens* powder and add it to a 60%–80% ethanol solution, extract it 2–4 times by heating in a water bath at 70–80°C, combine the filtrates, concentrate under reduced pressure, volatilize until there is no alcohol odor, add 0.8–1.2 times the mass of butanediol, stir evenly, filter, and obtain the *Salvia splendens* extract, wherein the concentration, based on the crude drug, is 0.40–0.55 g / mL.

4. The skin care composition of Claim 1 or 2 wherein the composition is a skin care composition comprising a sunscreen agent. The preparation method of the peony root extract is as follows: pulverize dried peony roots, add 30% to 50% ethanol solution, heat and reflux at 75 to 90°C for 2 to 4 extractions, and combine the filtrates; concentrate the filtrate under reduced pressure, volatilize until no alcohol odor is detected, add 1.5 to 2.0 times the mass of the concentrate of glycerol, stir evenly, filter, and the peony root extract is obtained, wherein the concentration, based on the crude drug, is 0.25 to 0.40 g / mL.

5. A process for the preparation of the skin care composition according to claim 1 or 2, characterized in that, Mix sodium hyaluronate-ferulic acid graft, Salicornia glutinosa extract, and Paeonia lactiflora root extract together, and add water to make up to 100 parts.

6. The use of the skin care composition according to claim 1 or 2 in the preparation of a brightening and whitening skin care cosmetic.

7. A skin care cosmetic for lightening and whitening, characterized by, The cosmetic contains 0.5% to 30% of a skin care composition with brightening and whitening effects, wherein the skin care composition with brightening and whitening effects is prepared according to claim 5.

8. The skin care composition according to claim 7, wherein the composition is a skin lightening and whitening cosmetic composition. The cosmetic contains 2-20% of a skin care composition with brightening and whitening effects.

9. The skin care product for lightening and whitening according to claim 7 or 8, characterized by, The brightening and whitening skincare cosmetics mentioned are essence water, essence liquid, facial mask, essence lotion or essence cream.

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