Bacillus subtilis producing keratinase and its application and fermentation medium

By developing Bacillus subtilis SLXX08 with high keratinase activity and low cost, and optimizing the fermentation medium and conditions, the problems of poor thermal stability and high production cost in the prior art were solved, and efficient and safe keratinase production was achieved.

CN117946927BActive Publication Date: 2025-03-28HOPSHENGHUI (XINJIANG) BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202410148825.1
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-02-02
Publication Date
2025-03-28
Estimated Expiration
2044-02-02

AI Technical Summary

Technical Problem

In the prior art, keratinases with poor thermal stability cannot be widely used in the industry, and the production cost of high-yield keratinases is high and unsafe and reliable.

Method used

Bacillus subtilis SLXX08, which has high keratinase-producing activity and low cost, was developed to improve enzyme yield and stability by optimizing fermentation medium and conditions.

Benefits of technology

High yield of keratinase (above 1560U/mL), reducing production costs, and improving the thermal stability and application safety of the enzyme.

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Abstract

The present invention provides a keratinase-producing Bacillus subtilis and an application and a fermentation medium thereof, belonging to the field of microorganisms. A keratinase-producing Bacillus subtilis SLXX08 was deposited in the General Microbiological Center of the China Microbiological Culture Collection Administration on May 5, 2023, with a deposit number of CGMCC No.27266. An application of a keratinase-producing Bacillus subtilis SLXX08 is an application in degrading feathers and removing animal fur. The keratinase-producing Bacillus subtilis and an application and a fermentation medium provided by the present invention have high keratinase activity, low enzyme production cost, and are safe and reliable.
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