A kind of Streptococcus suis exuviae and its preparation method and application

By constructing the endogenous cell wall perforation plasmid of Streptococcus suis and using the secondary inactivation method of using the cell penetrating peptide WR12, the problem of difficulty in preparing Streptococcus suis in the prior art was solved, efficient preparation and 100% inactivation were achieved, and the immunogenicity and application potential of the vaccine were improved.

CN117965592BActive Publication Date: 2025-03-28NANJING AGRICULTURAL UNIVERSITY
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Patent Information

Application Number
CN202410119509.1
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-01-29
Publication Date
2025-03-28
Estimated Expiration
2044-01-29

AI Technical Summary

Technical Problem

The prior art is difficult to effectively prepare the gram-positive bacteria Streptococcus suis, resulting in limited vaccine development and application.

Method used

By constructing the endogenous cell wall perforation plasmid of Streptococcus suis, using the tetracycline-induced regulation system and the expression of the cell wall perforation enzyme Crfp, combined with the secondary inactivation method of cell penetration peptide WR12, the preparation and 100% inactivation of Streptococcus suis were achieved.

Benefits of technology

It has achieved efficient preparation and 100% inactivation of Streptococcus suis molt, effectively retaining the richness and integrity of Streptococcus suis surface antigens, and improving the immunogenicity and application potential of the vaccine.

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Abstract

The present invention discloses a Streptococcus suis exuviae and a preparation method and application thereof. The preparation method of the Streptococcus suis exuviae comprises the following steps: a plasmid containing a tetracycline-induced regulatory system as shown in a nucleotide sequence such as SEQ ID NO.1 and a Streptococcus suis cell wall perforating enzyme Crfp as shown in a nucleotide sequence such as SEQ ID NO.2 is transferred into Streptococcus suis and then cultured, and the Streptococcus suis exuviae is obtained after inducing the expression of Crfp by anhydrotetracycline. Experimental verification shows that the addition of anhydrotetracycline can induce the expression of CrfP to produce Streptococcus suis exuviae, and the additional addition of penetrating peptide WR12 can promote the yield of exuviae and achieve complete inactivation of Streptococcus suis. The preparation of exuviae by the present invention has a simple technical process, no chemical inactivation reagent is added in the whole process, the surface antigen activity is fully retained, and the immunogenicity is strong, which lays a foundation for the preparation of Streptococcus suis exuviae vaccine.
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