Noradrenaline fluorescent probe, preparation method thereof and application thereof in cell imaging and biosensing

CN118056824BActive Publication Date: 2026-10-16EAST CHINA NORMAL UNIV
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Patent Information

Application Number
CN202211456955.9
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-11-21
Publication Date
2026-10-16
Estimated Expiration
2042-11-21

AI Technical Summary

Technical Problem

到目前为止，设计用于快速、特异和敏感的NE检测的荧光探针仍然是一项具有挑战性的任务

Benefits of technology

[0038] The beneficial effects of this invention include: First, it synthesizes near-infrared fluorescent probes 2a-5a and 2b-5b with high selectivity for NE. The NE fluorescent probe 2a exhibits a significant change in fluorescence ratio under 650 nm excitation, which can correct for environmental interference, improve the accuracy of NE quantitative analysis, and enrich detection methods. In the preparation method of this invention, two structurally different hemiflora fluorophores are first synthesized. Then, an aromatic group containing thiocarbonate as a recognition functional group is introduced into the fluorophore structure to construct a novel NE fluorescent probe. When NE is present in solution, the NE fluorescent probe can react rapidly with NE, the recognition functional group is cleaved, and the fluorophore fluorescence is restored. Therefore, fluorescence detection of NE is achieved, thereby further enabling rapid detection of NE within cells.

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Abstract

The application discloses a near-infrared fluorescent probe for detecting norepinephrine (NE), a preparation method of the near-infrared fluorescent probe and application of the near-infrared fluorescent probe in cell imaging and biosensing, and belongs to the technical field of fluorescent imaging and biosensing. The application further discloses a preparation method of the NE near-infrared fluorescent probe. Two different semi-fluorophores are synthesized first, then aromatic groups containing thiocarbonic ester as a recognition functional group are introduced to the structure of the fluorophore to construct eight different novel NE fluorescent probes (2a-5a and 2b-5b). When NE exists in a solution, the NE fluorescent probe can react with the NE rapidly, the recognition functional group is cleaved, and the fluorescence of the fluorophore is recovered, so that the fluorescence detection of the NE is realized, and the rapid detection of the NE in cells is further realized.
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