Preparation method of tobacco endogenous polysaccharide and application thereof in anti-tobacco mosaic virus

CN118255908BActive Publication Date: 2026-10-16YUNNAN TOBACCO COMPANY YUXI PREFECTURE COMPANY +1
0 Cites 0 Cited by

Patent Information

Application Number
CN202410367834.X
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-03-28
Publication Date
2026-10-16
Estimated Expiration
2044-03-28

AI Technical Summary

Technical Problem

[0005]本发明解决目前关于烟草内源多糖的研究报道较少，特别是对烟草内源多糖的分离纯化及其应用方面的研究目前还尚处于空白阶段的问题，提供一种烟草内源多糖的制备方法及其在抗烟草花叶病毒中的应用

Benefits of technology

[0023]This invention determined the anti-TMV activity of endogenous polysaccharides against tobacco mosaic virus (TMV), demonstrating that these endogenous polysaccharides possess good anti-TMV activity and can effectively inhibit TMV polymerization in vitro. This addresses the current scarcity of research reports on tobacco endogenous polysaccharides, particularly regarding their application, which remains largely unexplored. It provides a material basis for further systematic research on the anti-TMV activity of tobacco endogenous polysaccharides and opens up a new channel for the field control of tobacco mosaic virus. This invention also provides a method for preparing tobacco endogenous polysaccharides. The polysaccharides are extracted from field tobacco waste. HPGPC analysis showed that the homogeneous polysaccharides are highly uniform, with a molecular weight of 3061.328 Da. This method also addresses the current scarcity of research reports on tobacco endogenous polysaccharides, particularly regarding their preparation methods. The extraction rate using the method provided by this invention is approximately 4.2%, maximizing the enrichment of the active ingredient, tobacco endogenous polysaccharides, and effectively ensuring the application of the endogenous anti-TMV active component.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The present application belongs to the technical field of tobacco mosaic virus control, and particularly relates to a preparation method of tobacco endogenous polysaccharide and application of the tobacco endogenous polysaccharide in resisting tobacco mosaic virus. The present application provides the preparation method of the tobacco endogenous polysaccharide, which comprises the following steps: S1: tobacco waste is crushed, decolorized and impurity-removed; S2: tobacco endogenous crude polysaccharide is extracted; S3: the tobacco endogenous crude polysaccharide is concentrated; S4: the tobacco endogenous crude polysaccharide is deproteinized; and S5: the tobacco endogenous crude polysaccharide is purified. The tobacco endogenous polysaccharide prepared by the present application can prevent tobacco mosaic virus, is a homogeneous polysaccharide with a molecular weight of 3061.328 Da as determined by HPGPC, has an extraction rate of about 4.2%, and effectively ensures application of active components of the tobacco endogenous polysaccharide in resisting tobacco mosaic virus.
Need to check novelty before this filing date? Find Prior Art