A composition and method for culturing signal organizers and micro-organs using the same
Human pluripotent stem cells were cultured using a combination of FGF2, BMP inhibitors, WNT signaling activators, and Notch pathway inhibitors to generate signal organizers and induce microorganisms. This solved the problems of organogenesis and early pregnancy drug screening in existing technologies, and enabled the generation of organ progenitor cells and microorganisms as well as early pregnancy drug screening.
Patent Information
- Application Number
- CN202410171326.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-02-06
- Publication Date
- 2026-07-24
- Estimated Expiration
- 2044-02-06
AI Technical Summary
Existing technologies struggle to obtain signal organizers and induce the formation of various human organs, especially in an in vitro environment. Furthermore, existing organoid systems have not been able to fully replace treatment or transplantation and have limited effectiveness in screening drugs in early pregnancy.
Human pluripotent stem cells were cultured using a combination of FGF2, BMP inhibitors, WNT signaling activators, and Notch pathway inhibitors to generate signal organizers, which were then transplanted into mammals to induce microorganism generation.
Various organ precursor cells and micro-organs were successfully generated in vitro and in vivo, simulating the organ development process of early human embryos, providing experimental sources for drug screening in early pregnancy, and generating relatively complete organ morphologies.