A bionic gastric microtissue electrochemical sensor and its preparation method and application

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Bionic gastric microtissue model was prepared through biological 3D printing technology and combined with electrochemical sensing technology to construct a bionic gastric microtissue electrochemical sensor, which solved the problem that traditional detection methods could not meet the on-site detection and simulate the allergic environment in the body, and achieved rapid and accurate detection of ovalbumin.

CN118408985BActive Publication Date: 2025-03-21NANJING UNIV OF FINANCE & ECONOMICS

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Patent Information

Application Number: CN202410469477.8
Authority / Receiving Office: CN · China
Patent Type: Patents(China)
Current Assignee / Owner
Filing Date: 2024-04-18
Publication Date: 2025-03-21
Estimated Expiration: 2044-04-18

Application Information

Patent Timeline

18 Apr 2024

Application

CN202410469477.8

21 Mar 2025

Publication

CN118408985B

IPC: G01N27/327; G06T17/00; G06F30/10; B29C64/10; B33Y80/00; G06F113/10

CPC: G01N27/3275; G01N27/3277; G06T17/00; G06F30/10; B29C64/10; B33Y80/00; G06F2113/10

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Geometric CAD Additive manufacturing apparatus

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AI Technical Summary

Technical Problem

Most cellular sensors are difficult to simulate the allergic environment in the body, and traditional detection methods cannot meet the needs of on-site detection, which poses the problems of false positive risks and poor cost-effectiveness.

Method used

A high-throughput, standardized bionic gastric microtissue model was prepared by a projection photocuring biological 3D printer, and covered it on the modified screen-printed electrode to construct a bionic gastric microtissue electrochemical sensor to achieve rapid detection of ovalbumin.

Benefits of technology

It realizes rapid, accurate and stable detection of ovalbumin, reduces manual operation errors, improves the stability and reproducibility of the sensor, and is suitable for on-site detection.

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Abstract

The present invention discloses a bionic gastric microtissue electrochemical sensor and its preparation method and application, which belongs to the technical field of electrochemical sensors and food safety detection. The present invention uses a projection-type light-curing biological 3D printer to prepare a bionic gastric microtissue model of a specific size in a high-throughput and standardized manner; then the bionic gastric microtissue is covered with gold nanoparticles (AuNPs), manganese dioxide (MnO 2 ), cerium-based metal organic framework (Ce‑MOF) material, and the working electrode of the screen-printed electrode modified with AAO membrane, and a bionic gastric microtissue electrochemical sensor is constructed by connecting to an electrochemical workstation; thereby achieving rapid detection of ovalbumin. The electrochemical sensor of the present invention is used to detect ovalbumin, with a detection limit of 0.042μg / mL and a detection range of 0.1μg / mL to 10.0μg / mL. The bionic gastric microtissue electrochemical sensor in the present invention is portable and is expected to be used for rapid on-site detection.

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