Heat stress resistant medicinal residue fermented feed and preparation method and application thereof

Abstract

The invention discloses a heat stress resistant medicinal residue fermented feed and a preparation method and application thereof, which belongs to the field of feed technology, and includes the following preparation raw materials: Lianhua Qingwen medicinal residue, Huoxiang Zhengqi water medicinal residue, Shidi Shui medicinal residue, plant raw materials, Lactobacillus paracasei, Bacillus subtilis, Neurospora crassa, Saccharomyces boulardii, Monascus, and composite enzyme. The fermented feed of the present invention uses a combination of Lianhua Qingwen medicinal residue, Huoxiang Zhengqi water medicinal residue, and Shidi Shui medicinal residue as composite medicinal residues, uses bean dregs, corn flour, and wheat bran as main matrices, uses Lactobacillus paracasei, Bacillus subtilis, Neurospora crassa, Saccharomyces boulardii, and Monascus as fermentation strains, uses cellulase, laccase, and protease as enzyme preparations, and produces laying hen feed with heat stress resistance, greatly improving the ability of laying hens to resist heat stress.

Description

Technical Field

[0001] The invention relates to the technical field of feed, and in particular to a heat stress resistant medicinal residue fermented feed and a preparation method and application thereof. Background Art

[0002] Chicken heat stress refers to a series of physiological reactions and symptoms caused by an increase in the body temperature of a chicken due to an excessively high ambient temperature that exceeds the heat tolerance range of the chicken. This usually occurs in late spring and early summer or in seasons when the climate suddenly becomes hot, as well as in chicken houses with excessive density and poor ventilation. The clinical characteristics of chicken heat stress include depression, coma, rapid breathing, heart failure, and in severe cases, shock and death of the animal. In addition, the symptoms of chicken heat stress also include rapid breathing, obvious panting, fluffy feathers, increased body temperature, increased water intake, loss of appetite, slow movement, lack of energy, red and swollen eyes, increased tears, diarrhea or constipation, fighting and pecking in the group, redness and swelling of the soles of the feet, and decreased egg production, or even cessation of egg production, which significantly affects the feed intake and production performance of the chicken.

[0003] With the development of the traditional Chinese medicine industry, the area of ​​traditional Chinese medicine cultivation has continued to expand, and the amount of traditional Chinese medicine residues has also gradually increased. The current treatment methods for residues are still mainly stacking, landfilling, and incineration, which not only causes waste of resources, but also causes environmental pollution to the surrounding soil, water and air. Residues are mainly waste generated after the extraction of plant roots, stems, leaves, fruits, animal viscera, minerals, etc. In addition to the active ingredients, the waste also contains rich nutrients such as cellulose, protein, polysaccharides, starch, fat, etc. Therefore, traditional Chinese medicine residues have a wide range of application value, but traditional Chinese medicine residues are difficult to use directly.

[0004] At present, there are many reports on the use of Chinese herbal medicine residues as feed additives, biofertilizers, and edible fungus substrates, but most of them have not classified the Chinese herbal medicine residues and only used the nutrients in the residues without using their medicinal value. How to make full use of the nutrients and medicinal components in the residues has become an important issue.

[0005] Patent CN114788548A discloses a fermented feed of fermented slag of Astragalus, Angelica, Leonurus, Licorice, Dandelion and Tangerine Peel to improve the egg production rate of chickens, improve the yolk color and egg quality, and enhance the body's immune function, antioxidant capacity and disease resistance. However, Chinese medicine decoction is mostly boiled in the form of a formula, and it is difficult to classify and recycle the residues, which exist in a mixed form. Therefore, the Chinese medicine residue treatment needs to be recycled in the form of a formula.

[0006] Patent CN115553378 A discloses a Chinese medicine residue feed and a preparation method thereof. The Chinese medicine residue consisting of the residue of Shengxuebao mixture, Fritillaria cirrhosa and Achyranthes bidentata, combined with bacterial fermentation, can promote the growth of poultry and livestock such as chickens, enhance the immunity of poultry and livestock and improve the weight gain effect. However, it does not disclose the effect of the prepared Chinese medicine residue feed on heat stress performance.

[0007] Patent CN107373086A discloses a health-care feed additive produced using Chinese medicine residues and a preparation method. The patent uses the residues of heat-clearing and detoxifying prescriptions, tonic prescriptions, and blood-tonifying prescriptions as fermentation substrates, and uses Aspergillus niger, Trichoderma reesei, Saccharomyces cerevisiae, Candida utilis, Bacillus natto, and Acetobacter for synergistic fermentation, thereby enhancing animal nutrition and improving animal disease resistance. However, it does not disclose the effects of the health-care feed additive prepared by the patent on heat stress performance.

[0008] In view of this, this application is filed. Summary of the invention

[0009] The purpose of the present invention is to overcome the shortcomings of the prior art and provide a heat stress resistant medicinal residue fermented feed and a preparation method and application thereof. The fermented feed has excellent heat stress resistant effect.

[0010] To achieve the above object, the technical solution adopted by the present invention is:

[0011] A heat stress resistant medicinal residue fermented feed comprises the following raw materials in parts by weight: 100-200 parts of Lianhua Qingwen medicinal residue, 80-300 parts of Huoxiang Zhengqi Shui medicinal residue, 30-150 parts of Shidi Shui medicinal residue, 150-580 parts of plant raw materials, 3-8 parts of Lactobacillus paracasei, 10-15 parts of Bacillus subtilis, 0.5-3 parts of Neurospora crassa, 0.1-5 parts of Saccharomyces boulardii, 1-5 parts of Monascus, and 4.3-19 parts of complex enzymes.

[0012] The present invention creatively combines the above-mentioned raw materials according to a specific ratio to obtain a fermented feed with excellent anti-heat stress effect. The fermented feed uses a combination of Lianhua Qingwen medicinal residue, Huoxiang Zhengqishui medicinal residue and Shidi Shui medicinal residue as composite medicinal residues, uses bean dregs, corn flour and wheat bran as main matrices, uses Lactobacillus paracasei, Bacillus subtilis, Neurospora crassa, Saccharomyces boulardii and Monascus as fermentation strains, and uses cellulase, laccase and protease as enzyme preparations. Under the synergistic effect of various components, the medicinal value and nutritional value of the medicinal residues are fully utilized, the nutritional value of the fermented feed is effectively improved, sufficient nutrients can be provided for microbial fermentation, the progress of microbial fermentation is accelerated, and laying hen feed with anti-heat stress effect is produced, which greatly improves the ability of laying hens to resist heat stress. At the same time, the inventor unexpectedly finds when detecting egg indicators that the fermented feed can also improve the eggshell strength.

[0013] The inventors of the present invention have found in a large number of studies on bacteria selection that the type of bacteria has a significant impact on the effect. The present invention adopts specific weight portions of Lactobacillus paracasei, Bacillus subtilis, Neurospora crassa, Saccharomyces boulardii, and Monascus. Lactobacillus bulgaricus can reproduce in large quantities during the aerobic culture stage, and can be fermented to produce lactic acid under anaerobic conditions, and the fermented lactic acid is all L-lactic acid, which can be directly used by animals; Bacillus subtilis can reproduce in large quantities and form spores during the aerobic culture stage, and can effectively improve the heat stress performance of laying hens after entering the body; Neurospora crassa grows and reproduces rapidly, and the fermentation product is rich in carotene, and the fungus has a strong ability to produce cellulase, which can promote the hydrolysis of medicinal residues; Saccharomyces boulardii is an intestinal probiotic, which can effectively improve the balance of intestinal flora and enhance the body's immunity; the Monascus fermentation product contains protease, amylase, r-aminobutyric acid, red pigment, Monacolin K, etc., protease and amylase can promote the decomposition of fermentation matrix, promote microbial utilization, and improve feed utilization value. The r-aminobutyric acid produced by fermentation can effectively improve the heat stress ability of laying hens; through the synergistic effect of Lactobacillus paracasei, Bacillus subtilis, Neurospora crassa, Saccharomyces boulardii, and Monascus, each bacteria interacts with each other and cooperates with each other to fully hydrolyze the drug residue and effectively decompose the fermentation matrix, which can make full use of the nutrients of the drug residue and the matrix, effectively improve the utilization rate of nutrients, increase the fermentation rate and fermentation progress, and effectively improve the anti-heat stress effect.

[0014] The inventors of the present invention have found that, under the specific system of the present invention, the amount of raw materials has a great influence on the heat stress effect. The present invention significantly improves the heat stress effect by controlling the amount of each raw material. If the amount of the raw materials deviates from the scope of the present invention, the heat stress effect will be significantly reduced. The inventors speculate that when the amount of raw materials deviates from the scope of the present invention, the nutrient utilization rate of the fermentation substrate is low, the microbial fermentation is slow, the feed utilization rate is low, and the various components are difficult to play an ideal synergistic role, and even antagonism occurs, which leads to a significant decrease in the effect.

[0015] As a preferred embodiment of the present invention, the preparation raw materials include the following parts by weight: 130 parts of Lianhua Qingwen medicinal residues, 200 parts of Huoxiang Zhengqi medicinal residues, 80 parts of Shidi Shui medicinal residues, 390 parts of plant raw materials, 5 parts of Lactobacillus paracasei, 12 parts of Bacillus subtilis, 1.8 parts of Neurospora crassa, 4 parts of Saccharomyces boulardii, 3 parts of Monascus, and 9.5 parts of complex enzymes. In particular, when the dosage of each raw material is within this range, the anti-heat stress effect is significantly improved.

[0016] It should be noted that the Lianhua Qingwen dregs, Huoxiang Zhengqi dregs, and Shi Di Shui dregs described in the present invention are dregs obtained in the production process according to the prior art in the field. Specifically, the Lianhua Qingwen dregs can be dregs obtained from the production of Lianhua Qingwen capsules recorded in the 2020 edition of the Pharmacopoeia, the Huoxiang Zhengqi dregs can be dregs obtained from the production of Huoxiang Zhengqi oral liquid recorded in the 2020 edition of the Pharmacopoeia, and the Shi Di Shui dregs can be dregs obtained from the production of Shi Di Shui recorded in the 2020 edition of the Pharmacopoeia.

[0017] As a preferred embodiment of the present invention, the composite enzyme comprises cellulase, laccase and protease, and the weight ratio of the cellulase, laccase and protease is (0.3-6): (1-5): (3-8).

[0018] As a preferred embodiment of the present invention, the weight ratio of the cellulase, laccase and protease is 2:3.5:4.

[0019] As a preferred embodiment of the present invention, the plant raw materials include corn flour, bean dregs and wheat bran, and the weight ratio of the corn flour, bean dregs and wheat bran is (80-300): (50-200): (20-80).

[0020] As a preferred embodiment of the present invention, the weight ratio of the corn flour, bean dregs and wheat bran is 240:100:50.

[0021] More specifically, the heat stress resistant medicinal residue fermented feed described in the present invention includes the following raw materials in parts by weight: 100-200 parts by weight of Lianhua Qingwen medicinal residue, 80-300 parts by weight of Huoxiang Zhengqi water medicinal residue, 30-150 parts of Shidi Shui medicinal residue, 80-300 parts by weight of corn flour, 50-200 parts by weight of bean dregs, 20-80 parts by weight of wheat bran, 3-8 parts by weight of Lactobacillus paracasei, 10-15 parts by weight of Bacillus subtilis, 0.5-3 parts by weight of Neurospora crassa, 0.1-5 parts by weight of Saccharomyces boulardii, 1-5 parts by weight of Monascus, 0.3-6 parts by weight of cellulase, 1-5 parts by weight of laccase, and 3-8 parts by weight of protease.

[0022] Further preferably, the heat stress resistant medicinal residue fermented feed comprises the following raw materials in parts by weight: 130 parts by weight of Lianhua Qingwen medicinal residue, 200 parts by weight of Huoxiang Zhengqi medicinal residue, 80 parts by weight of Shidi Shui medicinal residue, 240 parts by weight of corn flour, 100 parts by weight of bean dregs, 50 parts by weight of wheat bran, 5 parts by weight of Lactobacillus paracasei, 12 parts by weight of Bacillus subtilis, 1.8 parts by weight of Neurospora crassa, 4 parts by weight of Saccharomyces boulardii, 3 parts by weight of Monascus, 2 parts by weight of cellulase, 3.5 parts by weight of laccase, and 4 parts by weight of protease.

[0023] The present invention also provides a method for preparing heat stress resistant medicinal residue fermented feed, comprising the following steps:

[0024] (1) Weighing each raw material according to the ratio, mixing the residue of Lianhua Qingwen, the residue of Huoxiang Zhengqishui, and the residue of Shidishui evenly, crushing, sieving, and obtaining a composite residue, and adjusting the water content of the composite residue to 40-60%;

[0025] (2) uniformly mixing the plant raw material and the composite medicinal residue to obtain a fermentation substrate;

[0026] (3) mixing Lactobacillus paracasei, Bacillus subtilis, Neurospora crassa, Saccharomyces boulardii, and Monascus purpurogenum to obtain a composite bacterial agent;

[0027] (4) adding the composite bacterial agent and the composite enzyme to the fermentation substrate, stirring evenly, and performing aerobic fermentation and anaerobic fermentation to obtain heat stress resistant medicinal residue fermented feed.

[0028] As a preferred embodiment of the present invention, the temperature of the aerobic fermentation is 28-32° C., and the time of the aerobic fermentation is 2-4 days.

[0029] As a preferred embodiment of the present invention, the temperature of the anaerobic fermentation is 28-32° C., and the time of the anaerobic fermentation is 10-12 days.

[0030] The invention also provides an application of the heat stress resistant medicinal residue fermented feed in preparing chicken feed.

[0031] The beneficial effects of the present invention are as follows: the fermented feed of the present invention uses a combination of Lianhua Qingwen medicinal residues, Huoxiang Zhengqishui medicinal residues and Shidi Shui medicinal residues as medicinal residues, uses bean dregs, corn flour and wheat bran as main matrices, uses Lactobacillus paracasei, Bacillus subtilis, Neurospora crassa, Saccharomyces boulardii and Monascus as fermentation strains, uses cellulase, laccase and protease as enzyme preparations, and with the synergistic effect of various components, fully utilizes the medicinal value and nutritional value of the medicinal residues, effectively improves the nutritional value of the fermented feed, can provide sufficient nutrients for microbial fermentation, accelerates the progress of microbial fermentation, produces laying hen feed with anti-heat stress effect, and greatly improves the ability of laying hens to resist heat stress. DETAILED DESCRIPTION

[0032] In order to make the purpose, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application are described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present application.

[0033] In the present application, the technical features described in an open manner include closed technical solutions composed of the listed features, and also include open technical solutions containing the listed features.

[0034] In this application, when it comes to numerical ranges, unless otherwise specified, the above numerical ranges are deemed to be continuous and include the minimum and maximum values ​​of the range, as well as each value between such minimum and maximum values. Further, when a range refers to an integer, each integer between the minimum and maximum values ​​of the range is included. In addition, when multiple ranges are provided to describe features or characteristics, the ranges can be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges included therein.

[0035] In the present application, there is no particular limitation on the specific dispersion and stirring treatment methods.

[0036] Unless otherwise specified, the components, raw materials or instruments used in the embodiments and comparative examples of the present invention are all commercially available raw materials or instruments, and the components and raw materials used in each parallel experiment are all of the same kind.

[0037] Among them, the residues of Lianhua Qingwen, Huoxiang Zhengqi Shui and Shidi Shui were provided by a Chinese medicine decoction factory in Bozhou, Anhui.

[0038] Lactobacillus paracasei was purchased from Beina Chuanglian Biotechnology Co., Ltd., strain number: BNCC192210.

[0039] Bacillus subtilis was purchased from China Industrial Microbiology Culture Collection, strain number: CICC 24713.

[0040] Neurospora crassa was purchased from China Industrial Microbiological Culture Collection Center, strain number: CICC 40203.

[0041] Saccharomyces boulardii was purchased from Biokeda Pharmaceuticals of France (Eco-live Saccharomyces boulardii powder).

[0042] Monascus was purchased from China Center for Type Culture Collection, strain number: CCTCC HF 2008631.

[0043] Saccharomyces cerevisiae was purchased from China Industrial Microbiological Culture Collection Center, strain number: CICC1355.

[0044] Bacillus natto was purchased from China Industrial Microbiological Culture Collection Center, strain number: CICC 20643.

[0045] Cellulase was purchased from Guangdong Yiduoli Biotechnology Co., Ltd., product number: C8.

[0046] Laccase was purchased from Ningxia Xiasheng Industrial Group Co., Ltd., product number: FDG-2264.

[0047] Protease was purchased from Guangdong Yiduoli Biotechnology Co., Ltd., product number: X-3002.

[0048] The following examples are provided to facilitate understanding of the present invention. These examples are not provided to limit the scope of the claims.

[0049] Examples 1 to 3, Comparative Examples 1 to 11

[0050] The formulas of the heat stress resistant medicinal residue fermented feeds of Examples 1 to 3 and Comparative Examples 1 to 11 are shown in Table 1 (all parts by weight) and Table 2 (all parts by weight).

[0051] The preparation method of the heat stress resistant medicinal residue fermented feed of Examples 1 to 3 and Comparative Examples 1 to 2 comprises the following steps:

[0052] (1) Weighing each raw material according to the ratio, mixing the residue of Lianhua Qingwen, the residue of Huoxiang Zhengqishui, and the residue of Shidishui evenly, crushing, passing the crushed material through a 1 cm sieve, crushing again, and replacing the sieve with a 0.5 cm sieve to obtain a composite residue, and removing part of the moisture in the composite residue with a dry-wet separator, and controlling the moisture content of the residue to 55% after dry-wet separation;

[0053] (2) mixing corn flour, bean dregs, wheat bran and the composite medicinal residues uniformly to obtain a fermentation substrate;

[0054] (3) mixing Lactobacillus paracasei, Bacillus subtilis, Neurospora crassa, Saccharomyces boulardii, and Monascus purpurogenum uniformly with a double cone mixer to obtain a composite bacterial agent;

[0055] (4) mixing cellulase, laccase and protease uniformly with a double cone mixer to obtain a composite enzyme;

[0056] (5) Add the composite bacterial agent and composite enzyme to the fermentation substrate, stir evenly, introduce sterile air at 30°C for aerobic fermentation for 3 days, then stop ventilation at 30°C and create an anaerobic environment for anaerobic fermentation for 11 days to obtain heat stress resistant medicinal residue fermented feed.

[0057] The difference between Comparative Example 3 and Example 1 is that Comparative Example 3 does not contain Lactobacillus paracasei, the total amount of bacteria remains unchanged, and the other aspects are the same.

[0058] The difference between Comparative Example 4 and Example 1 is that Comparative Example 4 does not contain Neurospora crassa, the total amount of bacteria remains unchanged, and the other contents are the same.

[0059] The difference between Comparative Example 5 and Example 1 is that Comparative Example 5 does not contain Saccharomyces boulardii, the total amount of bacteria remains unchanged, and the other contents are the same.

[0060] The difference between Comparative Example 6 and Example 1 is that Comparative Example 6 does not contain Monascus, the total amount of bacteria remains unchanged, and the other parts are the same.

[0061] The difference between Comparative Example 7 and Example 1 is that Comparative Example 7 uses an equal amount of Bacillus natto to replace Bacillus subtilis, and the other contents are the same.

[0062] The difference between Comparative Example 8 and Example 1 is that Comparative Example 8 uses an equal amount of Saccharomyces cerevisiae to replace Bacillus subtilis, and the other contents are the same.

[0063] The difference between Comparative Example 9 and Example 1 is that an equal amount of saccharomyces cerevisiae is used to replace Lactobacillus paracasei in Comparative Example 9, and the other contents are the same.

[0064] The difference between Comparative Example 10 and Example 1 is that Comparative Example 10 uses an equal amount of Lactobacillus bulgaricus to replace Monascus, and the other parts are the same.

[0065] The difference between Comparative Example 11 and Example 1 is that Comparative Example 11 does not contain laccase, the total amount of enzyme remains unchanged, and the other aspects are the same.

[0066] Table 1

[0067]

[0068]

[0069] Table 2

[0070]

[0071] Test Case

[0072] The fermented feed of medicinal residues was mixed with a basal diet (as shown in Table 3) in a mass ratio of 4:6 to form a laying hen feed. The laying hen feed was used in a laying hen breeding farm in Sihui City to carry out an experiment on the heat stress resistance of laying hens. 150-day-old Hy-Line brown laying hens with similar body weight and good health were selected as research subjects, and the laying hens were randomly divided into 16 groups (14 experimental groups, 1 blank control group, and 1 model control group. The experimental groups were fed with the feeds of Examples 1 to 3 and Comparative Examples 1 to 11, respectively. The blank control group was fed with a basal diet throughout the whole process, and the model control group was fed with a basal diet throughout the whole process). There were 60 chickens in each group (each group was divided into 3 small groups, each group had 20 chickens, and the test was conducted in parallel). The room was well ventilated. The experimental period was 28 days. The experimental feed was fed from 0 to 14 days, and the feeding temperature was controlled at 22±1℃. From 14 to 28 days, the Hy-Line brown laying hens except the blank control group were given a high temperature treatment for 6 hours every day. The temperature was slowly heated by a heater from 9 am to 33±1℃ at 10 am, and then slowly cooled from 16:00 in the afternoon to 22±1℃ at 17:00. The temperature was controlled at 22±1℃ from 17:00 to 9:00 the next day.

[0073] After the experiment, the indicators were tested and the average values ​​were taken (one maximum value and one minimum value were removed from each group).

[0074] Table 3 Basic diet formula (the premix is ​​provided by Bowen Group and is H043 premix)

[0075]

[0076] Table 4

[0077]

[0078]

[0079] It can be seen from Table 1 that the fermented feed of the present invention has excellent anti-heat stress effect and can effectively improve eggshell strength and egg production rate.

[0080] Comparative Examples 1 to 3 and Comparative Examples 1 to 2 show that by controlling the dosage of each raw material to: 100 to 200 parts of Lianhua Qingwen dregs, 80 to 300 parts of Huoxiang Zhengqishui dregs, 30 to 150 parts of Shidishui dregs, 150 to 580 parts of plant raw materials, 3 to 8 parts of Lactobacillus paracasei, 10 to 15 parts of Bacillus subtilis, 0.5 to 3 parts of Neurospora crassa, 0.1 to 5 parts of Saccharomyces boulardii, 1 to 5 parts of Monascus, and 4.3 to 19 parts of complex enzymes; the eggshell strength, heat stress resistance and egg production rate are significantly improved. .

[0081] By comparing Example 1 with Comparative Examples 3 to 10, it can be seen that the Lactobacillus paracasei, Bacillus subtilis, Neurospora crassa, Saccharomyces boulardii, and Monascus described in the present invention have a significant synergistic effect, can significantly improve the heat stress resistance and eggshell strength, and the lack of any one of them, or the use of other bacteria to replace them, all lead to a significant decrease in the heat stress resistance, eggshell strength, and egg production rate.

[0082] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A heat stress resistant medicinal residue fermented feed for laying hens, characterized in that: It is fermented from the following raw materials in parts by weight: 100-200 parts of Lianhua Qingwen medicinal residue, 80-300 parts of Huoxiang Zhengqi water medicinal residue, 30-150 parts of Shidi Shui medicinal residue, 150-580 parts of plant raw materials, 3-8 parts of Lactobacillus paracasei, 10-15 parts of Bacillus subtilis, 0.5-3 parts of Neurospora crassa, 0.1-5 parts of Saccharomyces boulardii, 1-5 parts of Monascus, and 4.3-19 parts of complex enzymes; The complex enzyme is composed of cellulase, laccase and protease, and the weight ratio of the cellulase, laccase and protease is (0.3-6): (1-5): (3-8); The plant raw material consists of corn flour, bean dregs and wheat bran, and the weight ratio of the corn flour, bean dregs and wheat bran is (80-300): (50-200): (20-80).

2. The heat stress resistant medicinal residue fermented feed for laying hens according to claim 1, characterized in that: It is fermented from the following raw materials in parts by weight: 130 parts of Lianhua Qingwen medicinal residues, 200 parts of Huoxiang Zhengqi medicinal residues, 80 parts of Shidi Shui medicinal residues, 390 parts of plant raw materials, 5 parts of Lactobacillus paracasei, 12 parts of Bacillus subtilis, 1.8 parts of Neurospora crassa, 4 parts of Saccharomyces boulardii, 3 parts of Monascus, and 9.5 parts of complex enzymes.

3. The heat stress resistant medicinal residue fermented feed for laying hens according to claim 1, characterized in that: The weight ratio of the cellulase, laccase and protease is 2:3.5:

4.

4. The heat stress resistant medicinal residue fermented feed for laying hens according to claim 1, characterized in that: The weight ratio of the corn flour, bean dregs and wheat bran is 240:100:

50.

5. The method for preparing the heat stress resistant fermented feed of medicinal residues for laying hens according to any one of claims 1 to 4, characterized in that: The following steps are involved: (1) Weigh each raw material according to the ratio, mix the Lianhua Qingwen residue, Huoxiang Zhengqi Shui residue, and Shidi Shui residue evenly, crush, and sieve to obtain a composite residue, and adjust the water content of the composite residue to 40-60%; (2) Evenly mixing the plant raw material and the composite medicinal residue to obtain a fermentation substrate; (3) Evenly mixing Lactobacillus paracasei, Bacillus subtilis, Neurospora crassa, Saccharomyces boulardii, and Monascus to obtain a composite bacterial agent; (4) Adding the compound bacterial agent and compound enzyme to the fermentation substrate, stirring evenly, and performing aerobic fermentation and anaerobic fermentation to obtain heat stress resistant medicinal residue fermented feed for laying hens.

6. The method for preparing the heat stress resistant fermented feed of medicinal residues for laying hens according to claim 5, characterized in that: The temperature of the aerobic fermentation is 28-32° C., and the time of the aerobic fermentation is 2-4 days.

7. The method for preparing the heat stress resistant fermented feed of medicinal residues for laying hens according to claim 5, characterized in that: The temperature of the anaerobic fermentation is 28-32° C., and the time of the anaerobic fermentation is 10-12 days.

8. Use of the fermented feed containing medicinal residues for laying hens to resist heat stress according to any one of claims 1 to 4 in preparing feed for laying hens.

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