Osmanthus fragrans OfNAC94 gene and its expression protein and application
By providing the osmanthus OfNAC94 gene and its expression protein and overexpressing it in large-flower tobacco, the petal color was successfully regulated, and the problem of difficulty in regulating the osmanthus color in the prior art was solved, which significantly improved the ornamentality of osmanthus.
CN118638809BActive Publication Date: 2025-03-28NANJING FORESTRY UNIV
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Patent Information
- Application Number
- CN202410859532.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-28
- Publication Date
- 2025-03-28
- Estimated Expiration
- 2044-06-28
AI Technical Summary
Technical Problem
The existing technology is difficult to effectively regulate the genes of osmanthus color, which affects the ornamental value and breeding process of osmanthus.
Method used
The osmanthus OfNAC94 gene and its expression protein are provided, and the expression vector is constructed and the transformation of large-flower tobacco is transformed, and the transgenic plants with lighter petal color are screened to achieve the function of controlling the color of flowers.
Benefits of technology
A transgenic large-flower tobacco plant that overexpresses the OfNAC94 gene was successfully constructed, and the petal color became significantly lighter, solving the technical problem of regulating the color of osmanthus.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention discloses an osmanthus OfNAC94 gene, its expression protein and application, and relates to the technical field of plant genetic engineering. The osmanthus OfNAC94 gene disclosed by the invention has a nucleotide sequence as shown in SEQ ID NO.1, and an amino acid sequence of its expression protein as shown in SEQ ID NO.2. The invention constructs an expression vector Super1300‑OfNAC94 of the osmanthus OfNAC94 gene; transforms the constructed expression vector Super1300‑OfNAC94 of the osmanthus OfNAC94 gene into Nicotiana tabacum; cultivates, screens and obtains transgenic Nicotiana tabacum plants with lighter petal colors. Compared with EV (transferred into Super1300 empty vector), the transgenic Nicotiana tabacum plants overexpressing the OfNAC94 gene constructed by the invention have significantly lighter petal colors.
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