Torreya grandis TgGPAT6 gene, cloning and screening methods, and its application in regulating plant oil accumulation

By screening and cloning the TgGPAT gene of TgGPAT, it verifies its function of regulating oil accumulation, solves the problem of regulating oil accumulation and control of turmeric fruits, improves the oil content of plants, and provides gene candidates for improving the quality of turmeric.

CN119040345BActive Publication Date: 2025-03-25ZHEJIANG FORESTRY UNIVERSITY
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Patent Information

Application Number
CN202411342364.8
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-09-25
Publication Date
2025-03-25
Estimated Expiration
2044-09-25

AI Technical Summary

Technical Problem

The prior art is difficult to effectively regulate the accumulation of oil in torreya fruits, affecting the quality and nutritional value of the fruit.

Method used

The TgGPAT gene was screened out from the Torreya transcriptome, and its coding region sequence was obtained through clonal screening method, and subcellular localization and expression analysis were performed to verify its function of regulating vegetable oil accumulation.

Benefits of technology

It has successfully increased the oil content in plant leaves, provided candidate genes for genetic improvement of targeted quality of Torreya and cultivating excellent germplasm, and broadened the scope of Torreya genetic resources.

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Abstract

The invention discloses a Torreya grandis TgGPAT6 gene, a cloning and screening method, and an application in regulating plant oil accumulation. The invention screens out a TgGPAT gene that may regulate oil accumulation from the Torreya grandis transcriptome, and transient tobacco experiments prove that the TgGPAT gene can increase the oil content in plant leaves, and can be applied to the directional quality genetic improvement of Torreya grandis and the cultivation of new high-quality germplasm, broadening the scope of Torreya grandis genetic resources, providing candidate genes for the cultivation of high-quality Torreya grandis varieties, and also laying a foundation for in-depth research on the relevant mechanism of GPAT regulating oil accumulation.
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