Disinfectant for targeted killing of African swine fever virus and application thereof

Abstract

The invention provides a hog house disinfectant for targeted killing of African swine fever viruses and application of the hog house disinfectant in the field of disinfectants, and the hog house disinfectant for targeted killing of the African swine fever viruses is prepared by uniformly mixing the following components in parts by weight: 4-6 parts of polyvinyl alcohol, 2-3 parts of cinnamyl aldehyde and 2.5 parts of a nano-silver aqueous solution with the concentration of 100mg / L. It is found for the first time that cinnamyl aldehyde and nano-silver have a synergistic effect, have a targeted killing effect on African swine fever viruses, and can be used for disinfecting hog houses and killing or preventing the African swine fever viruses.

Description

Technical Field

[0001] The present invention relates to a virus-killing disinfectant, and in particular to a disinfectant for targeted killing of African swine fever virus. Background Art

[0002] In the Chinese patent database, a nano dry powder for making the floor of livestock and poultry houses have disinfection and self-cleaning functions and an application method thereof are disclosed, and its publication number is: CN111869680A; Publication date: 20201103; The nano dry powder for making the floor of livestock and poultry houses have disinfection and self-cleaning functions includes the following components: 0.011-0.22wt% of nano silver, 0.5-1.0wt% of silver nitrate premix, 0.75-1.5wt% of nano zinc oxide, 0.5-1.0wt% of glucose, 80.0wt% of montmorillonite and 16.28-18.239wt% of bran. The silver nitrate premix is ​​prepared by dissolving and diluting chemically pure silver nitrate, spraying it, and adsorbing it with bran to make it evenly premixed. The silver content in the silver nitrate premix is ​​1.0wt%. The disinfectant dry powder is applied to the ground of the farm to make it disinfect and self-clean. The disinfection and self-cleaning ground has safe, slow-release, long-lasting and broad-spectrum bactericidal and virucidal effects, which can kill pathogenic microorganisms on the ground and in the environment of livestock and poultry houses, improve the biosafety level, and prevent and reduce the occurrence of diseases. Its disinfection and self-cleaning efficacy is based on the African swine fever virus as the reference standard, ensuring that the nano-silver saturation attack degree against the African swine fever virus is not less than 1 times, and the saturation attack degree of silver ions or single silver particles is 1.74×10 5 to 3.47×10 5 times.

[0003] Nano Silver is a metallic silver with a particle size of nanometers. It has a strong inhibitory and killing effect on dozens of pathogenic microorganisms such as Escherichia coli, gonococci, Chlamydia trachomatis, etc., and will not produce drug resistance. The existing nanosilver has a certain antiviral effect on African swine fever, but it only achieves the inhibitory effect of disinfection and self-purification of African swine fever virus by long-term inhibition of the growth of surface fungi, bacteria and viruses, and a good effect will only be achieved when the dosage of the drug is large. Summary of the invention

[0004] The purpose of the present invention is to provide a disinfectant that can kill African swine fever virus in a targeted manner. The disinfectant can kill African swine fever virus in a targeted manner, and can be used to disinfect pig houses, thereby cutting off the transmission pathway of African swine fever, and the amount of the drug used is small.

[0005] The object of the present invention is achieved in this way: a disinfectant for targeted killing of African swine fever virus comprises the following components in parts by weight: 4-6 parts of polyvinyl alcohol, 2-3 parts of cinnamaldehyde, and 2.5 parts of an aqueous solution of nanosilver (n-Ag) with a concentration of 100 mg / L, which are evenly mixed to prepare a disinfectant (PVA-CIN-Ag composite disinfectant) for targeted killing of African swine fever virus.

[0006] Furthermore, the degree of polymerization of the polyvinyl alcohol is 1600-1800, and the degree of alcoholysis is 87-89%.

[0007] Furthermore, the average particle size of the nanosilver is 5-8 nanometers.

[0008] The disinfectant of the present invention can be used for disinfecting pig houses and specifically killing African swine fever viruses in vitro.

[0009] Compared with the prior art, the present invention has the following beneficial effects:

[0010] 1. It has a targeted killing effect on African swine fever virus and is used to kill African swine fever virus in vitro.

[0011] 2. In the prior art, cinnamaldehyde has a strong bactericidal and disinfecting effect, especially has a significant therapeutic effect on fungi. It has an inhibitory effect on Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Staphylococcus albus, Shigella dysenteriae, Bacillus typhi and paratyphoid A, Pneumococcus, Bacillus aerogenes, Proteus, Bacillus anthracis, Salmonella enteritidis, Vibrio cholerae, etc. It also has a significant bactericidal effect on Gram-positive bacteria and can be used to treat a variety of diseases caused by bacterial infections. However, there is no record of its effect on killing viruses. There is a synergistic effect between cinnamaldehyde and nano silver in the present invention, and under its synergistic effect, it has a significant synergistic killing effect on African swine fever virus.

[0012] 3. No residue will harm the health of pigs or affect the health of consumers.

[0013] The disinfectant of the present invention can be used for disinfecting pig houses to kill or prevent African swine fever virus. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 The scanning electron microscope SEM of the PVA-CIN-Ag composite disinfectant of the present invention Figure 1 .

[0015] Figure 2 The scanning electron microscope SEM of the PVA-CIN-Ag composite disinfectant of the present invention Figure 2 .

[0016] Figure 1 , Figure 2 In the figure, the round particles are clusters of nanosilver particles. DETAILED DESCRIPTION

[0017] Example 1

[0018] A disinfectant for killing African swine fever virus in a targeted manner is composed of the following components in parts by weight: 5 parts of polyvinyl alcohol (PVA), 2.8 parts of cinamaldehyde (cinamaldehyde, CIN purity>90%), and 2.5 parts of an aqueous solution of nanosilver (n-Ag) with a concentration of 100 mg / L. The components are evenly mixed to obtain a disinfectant for killing African swine fever virus in a targeted manner, namely, a PVA-CIN-Ag composite disinfectant.

[0019] The degree of polymerization of polyvinyl alcohol is 1700, the degree of alcoholysis is 88%, and the average particle size of nano silver is 5-8 nanometers.

[0020] The obtained disinfectant is used for disinfecting pig houses and killing or preventing African swine fever virus. The disinfectant of the present invention can effectively kill microorganisms in pig houses and inhibit bacteria for a long time without damaging the health of pigs and affecting the health of consumers due to residues, and is suitable for disinfecting pig houses in pig breeding.

[0021] Figure 1 It shows that n-Ag is evenly dispersed in the film and fills the gaps formed by CIN. Therefore, it can gradually form a film structure in the air when sprayed on the surface of the livestock house. Microscopic analysis shows that there are hydrogen bonds between n-Ag and PVA, forming physical cross-links, making the structure of the film more compact and improving the mechanical properties of the film. By forming a film on the surface of livestock house objects, it can inhibit the growth of fungi, bacteria and viruses on solid surfaces for a long time.

[0022] In the comparative experiment, the control group used two types of substances, one type was prepared with 5 parts of polyvinyl alcohol, 1, 2, 3, 4 and 5 parts of cinnamaldehyde respectively, and 2.5 parts of deionized water, a total of 5 samples, numbered as control group 1-5; the other type was prepared with 5 parts of polyvinyl alcohol and 2.5 parts of nanosilver aqueous solution with concentrations of 20 mg / L, 60 mg / L, 100 mg / L, 140 mg / L, and 180 mg / L respectively, a total of 5 samples, numbered as control group 6-10. The remaining conditions of the control group were consistent with those of Example 1.

[0023] The virus used in the experiment is African swine fever virus. The virus vector and corresponding experimental support facilities are selected, and the operation is carried out in accordance with relevant standards and operating procedures. The specific steps are as follows:

[0024] 1. Prepare African swine fever virus suspension: Select an identified African swine fever virus strain and prepare a virus suspension using centrifugation and filtration methods with a concentration of 10^6.5TCID50 / ml.

[0025] 2. Choose different disinfectants: Prepare disinfectants containing cinnamaldehyde, nanosilver, etc. in different concentrations according to the patent design.

[0026] 3. Conduct inactivation experiments: place a certain amount of African swine fever virus suspension in contact with disinfectants of different concentrations, collect samples after a certain period of time, and analyze the inactivation effect through methods such as virus titer tests.

[0027] Table 1 is a comparison table of the disinfectant of the present invention and the disinfectant of the control group.

[0028]

[0029] The results show that the PVA-CIN-Ag composite disinfectant has a better killing effect on African swine fever than the disinfectant prepared by simple nanosilver and cinnamaldehyde, and has a targeted killing effect. Comparing Example 1 and Control Group 8, although both use 100 mg / L of nanosilver, 2.5 parts, Example 1 has a significantly higher killing rate for African swine fever virus in 8 hours and 24 hours, indicating that Example 1 has a long-term killing effect; compared with Control Groups 9 and 10, Example 1 has a killing rate for African swine fever virus in 8 hours and 24 hours that is not weaker than Control Groups 9 and 10, but its nanosilver dosage is significantly less, indicating that the technical solution of Example 1 has a better technical effect; the above results show that cinnamaldehyde and nanosilver produce a synergistic technical effect.

[0030] Example 2

[0031] The disinfectant obtained in Example 1 was tested on different viruses, and the results are shown in Table 2 below:

[0032]

[0033] The above table shows that the disinfectant of the present invention has a targeted killing effect on African swine fever virus, but its killing effect on other viruses is not very obvious.

[0034] Example 3

[0035] Compared with Example 1, the dosage of nanosilver and polyvinyl alcohol was fixed, and only the dosage of cinnamaldehyde was changed. The experimental results are shown in Table 3 below:

[0036]

[0037]

[0038] The results showed that when the dosage of cinnamaldehyde was 2-3 parts, the effect was better. When it was lower than 2 parts, the killing effect on African swine fever virus decreased significantly. After the dosage was 3 parts, continuing to increase the dosage of cinnamaldehyde did not significantly enhance the killing effect on African swine fever virus. Therefore, it is appropriate to relax the dosage of cinnamaldehyde to 2-3 parts.

[0039] Example 4

[0040] The polyvinyl alcohol in the present invention is a film-forming agent, and changes in its dosage will also have a great impact on the killing of African swine fever virus. On the basis of other conditions in Example 1 being unchanged, only the amount of polyvinyl alcohol is changed, and experimental comparisons are performed, as shown in Table 4 below:

[0041]

[0042] The results showed that when polyvinyl alcohol was not used, the virus killing effect was the worst. As the amount of polyvinyl alcohol increased, the virus killing effect improved. The effect was best when 4-6 parts were used. If the amount of polyvinyl alcohol was continued to be increased, the virus killing effect was weakened.

[0043] The degree of polymerization of the polyvinyl alcohol used can be 1600-1800, and the degree of alcoholysis is 88%. Lowering the degree of alcoholysis will make it easier to dissolve in water, resulting in loss of effective ingredients. When the degree of alcoholysis is too high, the solubility of this type of polyvinyl alcohol in water is low, and usually requires a higher temperature to dissolve. When the degree of alcoholysis is 87% to 89%, this polyvinyl alcohol has better solubility at room temperature, but can maintain the long-term killing of African swine fever virus by the disinfectant.

[0044] The present invention is not limited to the above-mentioned embodiments. On the basis of the technical solution disclosed in the present invention, technicians in this field can make some substitutions and deformations to some technical features therein according to the disclosed technical content without creative labor, and these substitutions and deformations are all within the protection scope of the present invention.

Claims

1. A disinfectant for killing African swine fever virus, characterized in that: The invention comprises the following components in parts by weight: 4-6 parts of polyvinyl alcohol, 2-3 parts of cinnamaldehyde, and 2.5 parts of an aqueous solution of nano-silver with a concentration of 100 mg / L, which are evenly mixed to prepare a disinfectant capable of specifically killing African swine fever virus.

2. A disinfectant for killing African swine fever virus according to claim 1, characterized in that: The polyvinyl alcohol has a polymerization degree of 1600-1800 and an alcoholysis degree of 87-89%.

3. A disinfectant for killing African swine fever virus, characterized in that: The average particle size of the nanosilver is 5-8 nanometers.

4. The use of a disinfectant for targeted killing of African swine fever virus according to claim 1, characterized in that: Used for pig house disinfection and targeted killing of African swine fever virus in vitro.

Images