A method for directly inducing the regeneration of the whole plant of Limonium candidum

Soaking the petiole explant of the yellow flower saccharin by high-concentration hormone solution promotes the efficient differentiation and growth of its uncertain buds and roots, solving the problems of time-consuming and numerous steps in traditional tissue culture technology, and achieving rapid and direct regeneration of the whole plant.

CN119183962BActive Publication Date: 2025-03-28GUANGDONG OCEAN UNIVERSITY
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Patent Information

Application Number
CN202411633721.6
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-11-15
Publication Date
2025-03-28
Estimated Expiration
2044-11-15

AI Technical Summary

Technical Problem

The existing tissue culture technology of Huanghua Blood-enhancing Grass takes too long, has many steps and is prone to contamination, which seriously hinders its development.

Method used

The petiole explant of the yellow saccharin saccharin was soaked with high concentration hormone solution (40mg/L NAA, 30mg/L 6-BA, 10mg/L IBA solution) to promote efficient differentiation and rooting of uncertain buds, and then cultured in MS culture medium without hormone addition to achieve regeneration of the whole plant.

Benefits of technology

The regeneration time of the whole plant of Huanghua Blood-enhancing Grass is greatly shortened, and a complete plant with a height of 5-11cm and a root length of more than 6cm can be obtained in only 48 days. Compared with the traditional method, it saves 42-72 days and reduces the complexity of operation and the risk of pollution.

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Abstract

The present invention discloses a method for directly inducing the regeneration of the whole plant of Limonium chrysantha. The present invention divides the petioles of sterile Limonium chrysantha seedlings cultivated for 30 days into segments, and then uses high-concentration NAA, 6-BA, and IBA solutions to soak for 20-30 minutes, and then transfers them to MS culture medium for 48 days of induction culture, so as to obtain a regenerated complete plant differentiated from the petiole. The present invention does not require additional steps such as callus induction, adventitious bud elongation culture, and adventitious bud rooting culture, and can directly induce a regenerated complete plant with roots from an explant, providing a set of efficient solutions for the rapid propagation of Limonium chrysantha, and solving the problems of difficulty in obtaining sterile seedlings by tissue culture, long tissue culture cycle, cumbersome subculture and replacement culture procedures, and easy contamination of materials.
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