Preparation method of nucleic acid aptamer and detection reagent for gladiolus Burkholderia detection
By developing nucleic acid aptamers targeting C. Gladiolus C. and preparing dual-probe detection reagents, the problems of long detection time and low sensitivity in the existing detection methods are solved, and fast and sensitive detection is achieved, which is suitable for food safety monitoring needs.
CN119286870BActive Publication Date: 2025-03-25HEFEI UNIV OF TECH
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Patent Information
- Application Number
- CN202411664901.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-20
- Publication Date
- 2025-03-25
- Estimated Expiration
- 2044-11-20
AI Technical Summary
Technical Problem
The existing detection methods for Cyclotridiomycetes in foods lack effective identification elements, resulting in long detection time, low sensitivity, and are not suitable for on-site food safety monitoring.
Method used
A nucleic acid aptamer targeting C. Gladiolus C. was developed, and a dual-probe detection reagent was prepared to achieve rapid and sensitive detection by combining magnetic separation probes and fluorescent nanoenzyme probes.
Benefits of technology
It significantly improves detection efficiency, can complete sample analysis in a shorter time, has high sensitivity and specificity, is suitable for quickly responding to food safety monitoring needs, and reduces detection costs and experimental complexity.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention discloses a gladiolus Burkholderia aptamer and a screening method thereof, and belongs to the technical field of food safety. The DNA sequence of the gladiolus Burkholderia aptamer is shown in SEQ ID No: 1. The screening method uses computer-aided design, and quickly screens out aptamer candidate sequences that are highly specifically bound to bacterial membrane proteins from a large-scale nucleic acid sequence library through molecular docking technology, and then through in vitro experimental verification and optimization, finally obtains an aptamer with excellent affinity and specificity. The present invention overcomes the shortcomings of traditional aptamer screening methods such as long time consumption, high cost, and complex operation, and provides a new way to quickly and efficiently screen gladiolus Burkholderia specific recognition aptamers. The obtained aptamer is combined with a fluorescent nanozyme for the first time, and forms a dual probe structure with a magnetic bead loaded with concanavalin A (ConA), which can be used to construct a gladiolus Burkholderia rapid detection system, and has broad application prospects in the fields of food safety monitoring and pathogen detection.
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