A treatment method for promoting germination of Cinnamomum camphora seeds

Abstract

The present invention provides a processing method for promoting the germination of tea-fruit camphor seeds, and relates to the technical field of artificial propagation of tea-fruit camphor. The processing method mainly comprises the steps of removing the pseudocarp from fresh tea-fruit camphor fruits by ultrasonic treatment with ice water to obtain seeds, sudden cooling and temperature change pretreatment of the seeds, preparing nutrient solution with gibberellins, tocopherol acetate, N-acetylglucosamine, compound vitamins, and compound amino acids, spraying the nutrient solution with paper to wrap the seeds, sowing management, and the like. The present invention overcomes the shortcomings of the prior art, effectively improves the germination rate of fresh tea-fruit camphor seeds sown, and promotes the growth of early germinating seedlings of tea-fruit camphor, providing good guidance for the artificial propagation and planting of tea-fruit camphor.

Description

Technical Field

[0001] The invention relates to the technical field of artificial propagation of Cinnamomum camphora, and in particular to a treatment method for promoting germination of Cinnamomum camphora seeds. Background Art

[0002] Cinnamomum camphora is an evergreen tree of the genus Cinnamomum in the family Lauraceae. Cinnamomum camphora has a dense crown, beautiful tree shape, and is evergreen all year round. It is an excellent landscaping tree species and timber species. The fruit of Cinnamomum camphora is the largest of the Cinnamomum plants discovered so far and is the only tree species whose fruit can be eaten directly. Its kernel is rich in nutrients such as oil, protein, various minerals, and amino acids. Cinnamomum camphora is currently distributed in a narrow area in Yunlong County and Yangbi County of Dali Bai Autonomous Prefecture, Yunnan Province. The natural number is small, and it is difficult to see young trees and seedlings. Overall, natural reproduction in the wild is relatively difficult. Therefore, in order to protect germplasm resources, artificial breeding of Cinnamomum camphora is one of the important tasks at this stage.

[0003] Under natural conditions, the fruiting rate of Cinnamomum camphora is low, and the seeds of Cinnamomum camphora are composed of endocarp, cotyledon and embryo, which are large seeds. The endocarp of the seeds is very hard and has strong mechanical barrier effect. It has a dormancy period of up to 90-140 days, and it is difficult to germinate by direct sowing, so it is generally sown after mechanically breaking the endocarp at the base of the seeds of Cinnamomum camphora, which can make its germination rate reach about 35% in about 30 days. During this period, controlling the appropriate temperature and light conditions can effectively increase the germination rate to about 55%, but the overall germination rate is still low, so how to deal with fresh seeds of Cinnamomum camphora to comprehensively improve the germination rate is an important research direction for artificial breeding of Cinnamomum camphora at this stage. Summary of the invention

[0004] In view of the deficiencies in the prior art, the present invention provides a processing method for promoting the germination of Cinnamomum camphora seeds, which effectively improves the germination rate of fresh sowing seeds of Cinnamomum camphora and promotes the growth of early germinated seedlings of Cinnamomum camphora, providing good guidance for the artificial breeding and planting of Cinnamomum camphora.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0006] A method for promoting the germination of Cinnamomum camphora seeds, the method comprising the following steps:

[0007] S1. Acquisition of Cinnamomum camphora seeds: Select fresh Cinnamomum camphora fruits, wash them, mix them with ice water and ultrasonically vibrate them, then take them out and squeeze them to remove the false fruit skin, and obtain Cinnamomum camphora seeds for later use;

[0008] S2. Seed quenching and temperature change pretreatment: place the above-mentioned Cinnamomum camphora seeds in an environment of -5°C to -8°C for 10-15 minutes, then take them out and place them in ice water, then heat them to 25±2°C, continue to stand for 10-15 minutes, then take them out and dry them until the moisture content is 15%-20%, and the pretreated seeds are ready for use;

[0009] S3, nutrient solution preparation: clean water + gibberellin 100-200mg / L + tocopherol acetate 40-60mg / L + N-acetylglucosamine 100-120mg / L + complex vitamins 1-3g / L + complex amino acids 120-160mg / L;

[0010] S4, seed moistening treatment: spray a paper towel with the above nutrient solution, then wrap the pretreated seeds with the moistened paper towel and let stand at 25±2°C for 8-12h to obtain moistened seeds for use;

[0011] S5. Sowing: Sow the above moist seeds into the sterilized substrate, irrigate with clean water, and perform germination management to obtain Cinnamomum camphora seedlings.

[0012] Preferably, the power of the ultrasonic oscillation treatment in step S1 is 400-600 W, and the time is 2-3 min.

[0013] Preferably, the heating rate in ice water in step S2 is 3-5°C / min.

[0014] Preferably, the drying method in step S2 is hot air drying at 30±2°C.

[0015] Preferably, the complex vitamins in step S3 are vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin D3, and vitamin E mixed in a mass ratio of 2:0.6:0.2:1:1:3:0.4:0.8.

[0016] Preferably, the composite amino acids are lysine, methionine, valine and tryptophan in a mass ratio of 3:1:2:1.

[0017] Preferably, during the seed moistening treatment in step S4, nutrient solution is sprayed on the paper towel in a timely manner to ensure that the water content of the paper towel is 80%-120%.

[0018] Preferably, the matrix after disinfection in step S5 is obtained by spraying 500 times diluted 50% carbendazim wettable powder on the matrix and mixing well, covering with film and stacking for 1 day, and then uncovering the film and exposing to the sun for 2 days, and the matrix is ​​a mixture of humus soil, sandy loam and perlite.

[0019] Preferably, the conditions for germination management in step S5 are to control the temperature at 20-28°C, the relative humidity of the air at 70-80%, the daily light at 13-15h, and the light intensity at 15000Lx.

[0020] The present invention provides a treatment method for promoting the germination of camphor tree seeds, which has the advantages of:

[0021] The invention obtains fresh seeds by removing false fruit skin after ice-water bath ultrasonic vibration on fresh Cinnamomum camphora fruits at an early stage and then combines with subsequent rapid cooling and temperature change treatment, so as to effectively break the dormancy period of Cinnamomum camphora seeds, and subsequently uses a nutrient solution prepared by compounding gibberellins, tocopherol acetate, N-acetylglucosamine, complex vitamins and complex amino acids to soak paper towels and then wrap the seeds for treatment, so as to further promote the synchronous germination of Cinnamomum camphora seeds in a short time, and the total germination rate can reach more than 75% within 30 days, and at the same time can promote the growth of Cinnamomum camphora seedlings after germination, and comprehensively improve the efficiency of artificial breeding. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a bar graph of the germination of seeds in experimental groups 1-5 at 15d and 30d in the embodiment of the present invention;

[0023] Figure 2 This is a bar graph of the germination of seeds in experimental group 1 and experimental groups 6-8 at 15d and 30d in the embodiment of the present invention;

[0024] Figure 3 This is a schematic diagram of the growth of seedlings of the experimental group 1 seeds 95 days after sowing in the embodiment of the present invention;

[0025] Figure 4 This is a schematic diagram of the growth of seedlings of comparative example 1 in the embodiments of the present invention 95 days after sowing. DETAILED DESCRIPTION

[0026] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention is clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] In the following embodiments, the composite vitamins are obtained by mixing vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin D3, and vitamin E in a mass ratio of 2:0.6:0.2:1:1:3:0.4:0.8; the composite amino acids are obtained by compounding lysine, methionine, valine, and tryptophan in a mass ratio of 3:1:2:1.

[0028] The disinfection matrix is ​​prepared by mixing humus soil, sandy loam and perlite in a mass ratio of 3:2:1, spraying the matrix with 500 times diluted 50% carbendazim wettable powder and mixing well, covering the pile with film and stuffing it for 1 day, and then removing the film and exposing it to the sun for 2 days. Embodiment 1:

[0029] Preparation of different nutrient solutions:

[0030] Prepare different nutrient solutions according to the ingredients in Table 1 below:

[0031] Table 1

[0032] Embodiment 2:

[0033] Cinnamomum camphora seed germination treatment:

[0034] (1) Select fresh Cinnamomum camphora fruit, wash it, mix it with 5 times the volume of ice water, treat it with 400W ultrasonic vibration for 3 minutes, then take it out and squeeze it manually to remove the false fruit skin, and obtain Cinnamomum camphora seeds for later use;

[0035] (2) Sudden cooling and temperature change pretreatment: Place the above-mentioned Cinnamomum camphora seeds in an environment of -8°C for 10 minutes, then take them out and place them in ice water, and heat them to 25±2°C at a heating rate of 3-5°C / min, and continue to stand for 15 minutes, then take them out and dry them with hot air at 30±2°C until the moisture content is 15%-20%, and the pretreated seeds are ready for use;

[0036] (3) Seed moistening treatment: Spray a paper towel with nutrient solution, then wrap the pretreated seeds with the moistened paper towel and place them at 25±2℃ for 10 hours. During this period, spray the nutrient solution on the paper towel in time to ensure that the water content of the paper towel is 80%-120%. The seeds are moistened for use;

[0037] (4) Sow the above-mentioned moistened seeds into a sterilized substrate, irrigate with clean water, control the temperature at 20-28°C, the relative humidity of the air at 70-80%, the daily light intensity at 13-15 hours, and the light intensity at 15000Lx, perform germination management, and obtain Cinnamomum camphora seedlings.

[0038] 1. Using the above germination treatment method, set different sudden cooling and temperature change pretreatment conditions according to Table 2 below:

[0039] Table 2

[0040]

[0041] The nutrient solution in the above experimental groups 1-5 was nutrient solution 1;

[0042] Each experimental group sowed 200 seeds, and the seed germination rate of each group was recorded at 15d and 30d after sowing. The specific results are shown in Table 3 and Figure 1 As shown:

[0043] Table 3

[0044]

[0045] From the above tests, it can be seen that experimental groups 1-2 have better germination rates at 15 days, and the germination rates are all higher than 75% at 30 days, and the overall germination is more uniform.

[0046] 2. Using the above germination treatment method, set up various experimental groups with different nutrient solution treatments according to Table 2 below, sow 200 seeds in each experimental group, and test the germination rates of various experimental groups under different nutrient solution treatment conditions at 15d and 30d. The specific results are shown in Tables 4 and Figure 2 As shown:

[0047] Table 4

[0048]

[0049] It can be seen from Table 4 above that the seeds treated with nutrient solution 1 and nutrient solution 2 have a higher germination rate within 30 days.

[0050] Comparative Example 1:

[0051] Cinnamomum camphora seed germination treatment:

[0052] (1) Select fresh Cinnamomum camphora fruit, wash it, mix it with 5 times the volume of ice water, treat it with 400W ultrasonic vibration for 3 minutes, then take it out and squeeze it manually to remove the false fruit skin, and obtain Cinnamomum camphora seeds for later use;

[0053] (2) Sudden cooling and temperature change pretreatment: The above-mentioned Cinnamomum camphora seeds were placed at -8°C for 10 min, then taken out and placed in ice water, and then heated to 25±2°C at a heating rate of 3-5°C / min, and continued to be placed for 15 min, and then taken out and dried with hot air at 30±2°C until the moisture content is 15%-20%, and the pretreated seeds are ready for use;

[0054] (3) Seed moistening treatment: Place the pretreated seeds in nutrient solution 1 and soak them at 25±2℃ for 10 hours to obtain moist seeds for use;

[0055] (4) Sow the above-mentioned moistened seeds into a sterilized substrate, irrigate with clean water, control the temperature at 20-28°C, the relative humidity of the air at 70-80%, the daily light intensity at 13-15 hours, and the light intensity at 15000Lx, perform germination management, and obtain Cinnamomum camphora seedlings.

[0056] Detection:

[0057] 1. The germination rates of the seeds (200 seeds sown) in the comparative example 1 were tested at 15d and 30d. The specific results are shown in Table 5 below:

[0058] Table 5

[0059]

[0060] 2. 30 days after sowing, the seedlings with the same growth potential in the above experimental group 1 and comparative example 1 were marked, and the plant height of each group of seedlings after 65 days of growth (95 days after sowing) was recorded (5 plants were randomly selected from each group and the average value was recorded). The specific results are shown in Table 6 below:

[0061] Table 6

[0062]

[0063] From the above test, it can be seen that the use of nutrient solution soaked paper to wrap the seeds in Experimental Group 1 can achieve early seed germination and further improve the growth efficiency of seedlings compared with the direct soaking of seeds in nutrient solution in Comparative Example 1. Figure 3 and Figure 4 , Figure 3 The height of seedlings after germination in experimental group 1 was significantly higher than that in Figure 4 The seedlings of Comparative Example 1.

[0064] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for promoting the germination of Cinnamomum camphora seeds, characterized in that: The processing method comprises the following steps: S1. Acquisition of Cinnamomum camphora seeds: Select fresh Cinnamomum camphora fruits, wash them, mix them with ice water and ultrasonically vibrate them, then take them out and squeeze them to remove the false fruit skin, and obtain Cinnamomum camphora seeds for later use; S2. Seed quenching and temperature change pretreatment: place the above-mentioned Cinnamomum camphora seeds in an environment of -5°C to -8°C for 10-15 minutes, then take them out and place them in ice water, then heat them to 25±2°C, continue to stand for 10-15 minutes, then take them out and dry them until the moisture content is 15%-20%, and the pretreated seeds are ready for use; S3, nutrient solution preparation: clean water + gibberellin 100-200mg / L + tocopherol acetate 40-60mg / L + N-acetylglucosamine 100-120mg / L + complex vitamins 1-3g / L + complex amino acids 120-160mg / L; S4, seed moistening treatment: spray the paper towel with the above nutrient solution, then wrap the above pre-treated seeds with the moistened paper towel and let it stand at 25±2°C for 8-12h to obtain moistened seeds for use; during the seed moistening treatment, spray the paper towel with the nutrient solution in time to ensure that the water content of the paper towel is 80%-120%; S5. Sowing: Sow the above moist seeds into the sterilized substrate, irrigate with clean water, and perform germination management to obtain Cinnamomum camphora seedlings.

2. The processing method according to claim 1, characterized in that: The power of the ultrasonic oscillation treatment in step S1 is 400-600W, and the time is 2-3 minutes.

3. The processing method according to claim 1, characterized in that: The heating rate of placing in ice water in step S2 is 3-5°C / min.

4. The processing method according to claim 1, characterized in that: The drying method in step S2 is 30±2°C hot air drying.

5. The processing method according to claim 1, characterized in that: The vitamin complex in step S3 is obtained by mixing vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin D3 and vitamin E in a mass ratio of 2:0.6:0.2:1:1:3:0.4:0.

8.

6. The processing method according to claim 1, characterized in that: The composite amino acid is obtained by compounding lysine, methionine, valine and tryptophan in a mass ratio of 3:1:2:

1.

7. The processing method according to claim 1, characterized in that: The substrate after disinfection in step S5 is obtained by spraying 500 times diluted 50% carbendazim wettable powder on the substrate and mixing well, covering the substrate with a film and stacking it for 1 day, and then uncovering the film and exposing it to the sun for 2 days. The substrate is a mixture of humus soil, sandy loam and perlite.

8. The processing method according to claim 1, characterized in that: The conditions for germination management in step S5 are to control the temperature at 20-28° C., the relative humidity of the air at 70%-80%, the daily light at 13-15 h, and the light intensity at 15000 Lx.

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