A miR-iab-8 capable of regulating diapause of Asian corn borer and its application

By designing a regulated miR-iab-8 to target the Cycle gene, regulating the diapause status of Asian corn borers, solving the problem of difficulty in regulating diapause in the existing technology, and achieving the effect of affecting its normal development and leading to death.

CN119351406BActive Publication Date: 2025-03-21INNER MONGOLIA UNIV FOR THE NATITIES
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Patent Information

Application Number
CN202411894223.7
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-12-20
Publication Date
2025-03-21
Estimated Expiration
2044-12-20

AI Technical Summary

Technical Problem

The prior art is difficult to effectively regulate the diapause status of Asian corn borer, affecting its normal development and leading to death.

Method used

By designing a regulated miR-iab-8, targeting negatively regulates the Cycle gene, thereby regulating the expression of genes related to the biological clock signaling pathway, affecting the diapause status of Asian corn borer.

Benefits of technology

The regulation of the diapause status of Asian corn borer has been achieved, affecting its normal development and leading to death, and has great application prospects in the preparation of new nucleic acid pesticides.

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Abstract

The present invention discloses a miR‑iab‑8 capable of regulating the diapause of Asian corn borer and its application. Wherein, the nucleotide sequence of miR‑iab‑8 is shown in SEQ ID NO: 1. miR‑iab‑8 targets and negatively regulates the Cycle gene, thereby regulating the expression of genes related to the biological clock signaling pathway. The present invention achieves the silencing and overexpression of miR‑iab‑8 of Asian corn borer through inhibitors and agonists of miR‑iab‑8, thereby regulating the expression of the important gene Cycle in the growth and development process of Asian corn borer, resulting in the larvae being dysmorphically blocked and eventually dying. The present invention also provides the application of miR‑iab‑8 capable of regulating the diapause of Asian corn borer in preventing and controlling Asian corn borer or in the preparation of an agent for preventing and controlling Asian corn borer. The present invention can be used to prepare a new type of nucleic acid pesticide, and has great application prospects in the green control technology of Asian corn borer.
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