Acid-resistant fish protein glue as well as preparation method and application thereof

Abstract

The invention discloses acid-resistant fish protein glue as well as a method and application thereof, and belongs to the field of food. The method comprises the following steps: uniformly mixing fish protein glue and locust bean gum, and dissolving in water to obtain a fish protein glue-locust bean gum mixed solution; adjusting the pH value of the fish protein glue-locust bean gum mixed solution to 2-7 through acid or alkali to obtain a mixed solution; and sequentially carrying out homogenization treatment, high-pressure micro-jet treatment and drying treatment on the mixed solution to obtain the acid-resistant fish protein glue. According to the invention, homogenization treatment is combined with dynamic high-pressure microjet treatment to promote interaction of the fish protein glue and the locust bean gum, and compared with simple mixing of the fish protein glue and the locust bean gum, the gel performance of the fish protein glue is improved, so that the performance is improved under an acidic condition; the problem that the gel performance of the fish protein glue is weakened under the acidic condition is solved, the application range of the fish protein glue under the acidic condition is widened, and important value is provided for preparation of the high-gel-performance fish protein glue and product development.

Description

Technical Field

[0001] The present application belongs to the field of food, and specifically relates to an acid-resistant fish protein glue and a method and application thereof. Background Art

[0002] At present, most of the commercial protein glues in the world are mainly derived from mammalian pig skin, cow skin, pig bones and cow bones. However, due to the impact of mad cow disease and foot-and-mouth disease, the safety of mammalian protein glue (mammalian protein glue can transmit prion pathogens) has been questioned, and the application of mammalian protein glue is subject to certain restrictions. Fish protein glue has become the most promising substitute for mammalian protein glue because of its similar functional properties to mammalian protein glue.

[0003] Fish protein glue is mostly used in acidic food environments, such as yogurt, soft candy, etc. Electrolytes and acids in food systems are important ingredients commonly used in food, which can improve food flavor and extend food shelf life. However, the gel properties of protein glue are seriously weakened under acidic conditions, making fish protein glue still unable to replace mammalian protein glue for application in many fields. Summary of the invention

[0004] The purpose of the embodiments of the present application is to provide an acid-resistant fish protein glue and a method and application thereof, which can solve the problem that the gel properties of the protein glue are seriously weakened under acidic conditions.

[0005] In order to solve the above technical problems, this application is implemented as follows: In a first aspect, the present invention provides a method for preparing an acid-resistant fish protein glue, comprising the following steps: Mixing fish protein glue and locust bean gum and dissolving them in water to obtain a fish protein glue-locust bean gum mixed solution; The pH of the fish protein glue-locust bean gum mixed solution is adjusted to 2-7 by acid or alkali to obtain a mixed solution; The mixed solution is subjected to homogenization treatment, high-pressure microfluidization treatment and drying treatment in sequence to obtain the acid-resistant fish protein glue.

[0006] Optionally, the mass ratio of the fish protein glue to the locust bean gum is 10:0.1 to 5:1.

[0007] Optionally, the acid comprises a hydrochloric acid solution, and the concentration of the hydrochloric acid solution is 0.5 mol / L to 2 mol / L; The above-mentioned alkali includes sodium hydroxide solution, and the concentration of the sodium hydroxide solution is 0.5 mol / L~2 mol / L.

[0008] Optionally, the treatment conditions in the above homogenization process are: pressure 10 MPa to 50 MPa, treatment times 1 to 8 times; The treatment conditions in the above high-pressure microfluidic treatment process are: pressure 50 MPa to 250 MPa, and treatment times 1 to 9 times.

[0009] Optionally, the drying treatment is performed by spray drying; the treatment conditions during the spray drying process are: inlet air temperature 175°C~185°C, and outlet air temperature 80°C~100°C.

[0010] Optionally, after the step of mixing the fish protein glue and the locust bean gum and dissolving them in water, the preparation method further comprises: After the fish protein glue and locust bean gum are mixed and dissolved in water, they are placed in a 50 ℃ ~ 80 ℃ water bath and heated for 1 h ~ 2 h.

[0011] In a second aspect, an embodiment of the present application provides an acid-resistant fish protein glue prepared by the above-mentioned method for preparing the acid-resistant fish protein glue.

[0012] Optionally, the viscosity of the acid-resistant fish protein glue is 2 mpa.s to 5.5 mpa.s.

[0013] Optionally, the gel strength y of the acid-resistant fish protein glue and the pH value x of the mixed solution satisfy the following formula: y=-38.397x 2 +370.68x-172.89.

[0014] In a third aspect, the embodiments of the present application provide the use of the above-mentioned acid-resistant fish protein glue in the preparation of acidic food.

[0015] In the embodiments of the present application, the present invention uses a homogenization process combined with a dynamic high-pressure microfluidization process to promote the interaction between fish protein glue and locust bean gum, which improves the gel properties of fish protein glue compared to the simple mixing of fish protein glue and locust bean gum, thereby improving the performance under acidic conditions. In addition, the fish protein glue prepared according to the method of the present invention has a higher gel performance under acidic conditions, solves the problem of weakened gel performance of fish protein glue under acidic conditions, broadens the application range of fish protein glue under acidic conditions, and provides important value for the preparation of high-gel fish protein glue and product development. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0017] Figure 1This is a correlation equation between the gel strength of the acid-resistant fish protein glue and the pH value provided in some embodiments of the present application. DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0019] The acid-resistant fish protein glue provided in the embodiments of the present application, and its method and application are described in detail below through specific embodiments and their application scenarios in conjunction with the accompanying drawings.

[0020] In a first aspect, the present application provides a method for preparing an acid-resistant fish protein glue, comprising the following steps: Mixing fish protein glue and locust bean gum and dissolving them in water to obtain a fish protein glue-locust bean gum mixed solution; The pH of the fish protein glue-locust bean gum mixed solution is adjusted to 2-7 by acid or alkali to obtain a mixed solution; The mixed solution is subjected to homogenization treatment, high-pressure microfluidization treatment and drying treatment in sequence to obtain the acid-resistant fish protein glue.

[0021] The beneficial effects of the present invention are as follows: fish protein glue and locust bean gum are prepared into a mixed solution, and then the pH is adjusted to 2-7, and then the fish protein glue and locust bean gum are caused to interact by homogenization combined with dynamic high-pressure microfluidization. Compared with the simple mixing of fish protein glue and locust bean gum, the gel properties of fish protein glue are improved, and the performance under acidic conditions is improved. In addition, the present invention can produce a synergistic effect by adding locust bean gum to compound with fish protein glue, increase the viscosity of the system, gel strength, elasticity, etc.; however, the structure of fish protein glue is severely damaged under a strongly acidic environment (pH <3), resulting in a decrease in gel performance, and the addition of locust bean gum and the interaction between the two improves the gel performance of the system, and locust bean gum is not good under strongly acidic conditions, so a homogenization treatment is used to make the two substances interact better, and the improvement effect is more obvious under the same treatment.

[0022] In some embodiments, the mass ratio of the fish protein gel to the locust bean gum is 10: 0.1 to 5: 1. In the above preparation process, too large or too small a mass ratio of the fish protein gel to the locust bean gum will affect the improvement of the system gel performance.

[0023] In some embodiments, the acid comprises a hydrochloric acid solution, and the concentration of the hydrochloric acid solution is 0.5 mol / L to 2 mol / L; The above-mentioned alkali includes sodium hydroxide solution, and the concentration of the sodium hydroxide solution is 0.5 mol / L~2 mol / L.

[0024] Since the pH value of the mixed solution of fish protein glue and locust bean gum is about 5, according to the present invention, the pH value needs to be adjusted to 2-7 by using hydrochloric acid solution or sodium hydroxide solution.

[0025] In some embodiments, the treatment conditions in the above homogenization process are: pressure 10 MPa to 50 MPa, treatment times 1 to 8 times; The treatment conditions in the above high-pressure microfluidic treatment process are: pressure 50 MPa to 250 MPa, and treatment times 1 to 9 times.

[0026] In some embodiments, the drying process is spray drying; the treatment conditions during the spray drying process are: air inlet temperature 175°C-185°C, air outlet temperature 80°C-100°C. In the above preparation process, homogenization is beneficial to the stretching of the fish protein gel structure and can better interact with locust bean gum. If the above treatment conditions exceed the above range, the subsequent gel properties will be affected.

[0027] In some embodiments, after the above step of mixing fish protein glue and locust bean gum and dissolving them in water, the preparation method further comprises: After the fish protein glue and locust bean gum are mixed and dissolved in water, they are placed in a 50°C to 80°C water bath and heated for 1 h to 2 h. In the above preparation process, a temperature higher than 80°C will destroy the structure of the fish protein glue, and a temperature lower than 50°C will cause the locust bean gum to be unable to fully dissolve.

[0028] In the second aspect, the present application provides an acid-resistant fish protein glue prepared by the above-mentioned method for preparing the acid-resistant fish protein glue, wherein the viscosity of the acid-resistant fish protein glue is 2 mpa.s to 5.5 mpa.s. In the above-mentioned preparation process, the viscosity range is the viscosity range measured when adjusting the pH to 2-7, which has guiding significance for the application of subsequent products.

[0029] Beneficial effects: The acid-resistant fish protein glue prepared by the preparation method of the present invention has good gel properties under acidic conditions, which solves the problem that the gel properties of fish protein glue are far inferior to those of mammalian protein glue, and expands the application range of fish protein glue.

[0030] In some embodiments, the gel strength y of the acid-resistant fish protein glue and the pH value x of the mixed solution satisfy the following formula: y=-38.397x 2 +370.68x-172.89.

[0031] The present invention can calculate the gel strength of the fish protein glue at the pH value through the pH value of the mixed solution through the formula, so as to know the gel strength of the acid-resistant fish protein glue at a specific pH value, and then deduce the subsequent application occasions of the acid-resistant fish protein glue provided by the present invention through the pH value and the gel strength of the mixed solution.

[0032] In a third aspect, the present application provides an application of the above-mentioned acid-resistant fish protein glue in the preparation of acidic food.

[0033] Beneficial effects: The acid-resistant fish protein glue prepared by the present invention has high gelation under acidic conditions, which can solve the problem of weakened gelation performance of fish protein glue under acidic conditions, broaden the application scope of fish protein glue under acidic conditions, and provide important value for the preparation of high-gel fish protein glue and product development.

[0034] Example 1 A method for preparing an acid-resistant fish protein glue specifically comprises the following steps: (1) Weigh 6.67 g of fish protein gelatin and 0.3 g of locust bean gum and mix them evenly. Add 93.03 g of distilled water to the mixed sample and place it in a 60°C water bath for 3 h to obtain a fish protein gelatin-locust bean gum mixed solution. (2) adding 1 mol / L hydrochloric acid to the fish protein gelatin-locust bean gum mixed solution obtained in step (1) to adjust the pH of the fish protein gelatin-locust bean gum mixed solution to 2, thereby obtaining a mixed solution; (3) The mixed solution obtained in step (3) was subjected to homogenization treatment (pressure set to 50 MPa, treatment times 6 times), high-pressure microfluidization treatment (pressure set to 200 MPa, treatment times 8 times) and spray drying treatment (inlet air temperature of 180°C, outlet air temperature of 90°C) to obtain an acid-resistant fish protein glue with a viscosity of 4 mpa.s.

[0035] Example 2 A method for preparing an acid-resistant fish protein glue specifically comprises the following steps: (1) Weigh 6.67 g of fish protein gelatin and 0.3 g of locust bean gum and mix them evenly. Add 93.03 g of distilled water to the mixed sample and place it in a 60°C water bath for 3 h to obtain a fish protein gelatin-locust bean gum mixed solution. (2) adding 1 mol / L hydrochloric acid to the fish protein gelatin-locust bean gum mixed solution obtained in step (1) to adjust the pH of the fish protein gelatin-locust bean gum mixed solution to 2.8 to obtain a mixed solution; (3) The mixed solution obtained in step (3) was subjected to homogenization treatment (pressure set to 50 MPa, treatment times 6 times), high-pressure microfluidization treatment (pressure set to 200 MPa, treatment times 8 times) and spray drying treatment (inlet air temperature of 180°C, outlet air temperature of 90°C) to obtain an acid-resistant fish protein glue with a viscosity of 4 mpa.s.

[0036] Example 3 A method for preparing an acid-resistant fish protein glue specifically comprises the following steps: (1) Weigh 6.67 g of fish protein gelatin and 0.3 g of locust bean gum and mix them evenly. Add 93.03 g of distilled water to the mixed sample and place it in a 60°C water bath for 3 h to obtain a fish protein gelatin-locust bean gum mixed solution. (2) adding 1 mol / L hydrochloric acid to the fish protein gel-locust bean gum mixed solution obtained in step (1) to adjust the pH of the fish protein gel-locust bean gum mixed solution to 3.2 to obtain a mixed solution; (3) The mixed solution obtained in step (3) was subjected to homogenization treatment (pressure set to 50 MPa, treatment times 6 times), high-pressure microfluidization treatment (pressure set to 200 MPa, treatment times 8 times) and spray drying treatment (inlet air temperature of 180°C, outlet air temperature of 90°C) to obtain an acid-resistant fish protein glue with a viscosity of 4.8 mpa.s.

[0037] Example 4 A method for preparing an acid-resistant fish protein glue specifically comprises the following steps: (1) Weigh 6.67 g of fish protein gelatin and 0.3 g of locust bean gum and mix them evenly. Add 93.03 g of distilled water to the mixed sample and place it in a 60°C water bath for 3 h to obtain a fish protein gelatin-locust bean gum mixed solution. (2) adding 1 mol / L hydrochloric acid to the fish protein gelatin-locust bean gum mixed solution obtained in step (1) to adjust the pH of the fish protein gelatin-locust bean gum mixed solution to 4.5 to obtain a mixed solution; (3) The mixed solution obtained in step (3) was subjected to homogenization treatment (pressure set to 50 MPa, treatment times 6 times), high-pressure microfluidization treatment (pressure set to 200 MPa, treatment times 8 times) and spray drying treatment (inlet air temperature of 180°C, outlet air temperature of 90°C) to obtain an acid-resistant fish protein glue with a viscosity of 4.2 mpa.s.

[0038] Example 5 A method for preparing an acid-resistant fish protein glue specifically comprises the following steps: (1) Weigh 6.67 g of fish protein gelatin and 0.3 g of locust bean gum and mix them evenly. Add 93.03 g of distilled water to the mixed sample and place it in a 60°C water bath for 3 h to obtain a fish protein gelatin-locust bean gum mixed solution. (2) adding sodium hydroxide having a concentration of 1 mol / L to the fish protein gelatin-locust bean gum mixed solution obtained in step (1) to adjust the pH of the fish protein gelatin-locust bean gum mixed solution to 5.3, thereby obtaining a mixed solution; (3) The mixed solution obtained in step (3) was subjected to homogenization treatment (pressure set to 50 MPa, treatment times 6 times), high-pressure microfluidization treatment (pressure set to 200 MPa, treatment times 8 times) and spray drying treatment (inlet air temperature of 180°C, outlet air temperature of 90°C) to obtain an acid-resistant fish protein glue with a viscosity of 4 mpa.s.

[0039] Example 6 A method for preparing an acid-resistant fish protein glue specifically comprises the following steps: (1) Weigh 6.67 g of fish protein gelatin and 0.3 g of locust bean gum and mix them evenly. Add 93.03 g of distilled water to the mixed sample and place it in a 60°C water bath for 3 h to obtain a fish protein gelatin-locust bean gum mixed solution. (2) adding 1 mol / L sodium hydroxide to the fish protein gel-locust bean gum mixed solution obtained in step (1) to adjust the pH of the fish protein gel-locust bean gum mixed solution to 6.3 to obtain a mixed solution; (3) The mixed solution obtained in step (3) was subjected to homogenization treatment (pressure set to 50 MPa, treatment times 6 times), high-pressure microfluidization treatment (pressure set to 200 MPa, treatment times 8 times) and spray drying treatment (inlet air temperature of 180°C, outlet air temperature of 90°C) to obtain an acid-resistant fish protein glue with a viscosity of 5 mpa.s.

[0040] Example 7 A method for preparing an acid-resistant fish protein glue specifically comprises the following steps: (1) Weigh 6.67 g of fish protein gelatin and 0.3 g of locust bean gum and mix them evenly. Add 93.03 g of distilled water to the mixed sample and place it in a 60°C water bath for 3 h to obtain a fish protein gelatin-locust bean gum mixed solution. (2) adding sodium hydroxide having a concentration of 1 mol / L to the fish protein gelatin-locust bean gum mixed solution obtained in step (1) to adjust the pH of the fish protein gelatin-locust bean gum mixed solution to 5.3, thereby obtaining a mixed solution; (3) The mixed solution obtained in step (3) was spray-dried (the inlet air temperature was 180°C and the outlet air temperature was 90°C) to obtain an acid-resistant fish protein glue with a viscosity of 5 mPa.s.

[0041] Comparative Example 1 A method for preparing an acid-resistant fish protein glue specifically comprises the following steps: (1) Weigh 6.67 g of fish protein gelatin and add 93.33 g of distilled water, and place in a 60°C water bath for 3 h to obtain a fish protein gelatin solution; (2) adding 1 mol / L hydrochloric acid to the protein glue solution obtained in step (1) to adjust the pH of the fish protein glue solution to 2, thereby obtaining a hydrochloric acid-modified protein glue solution; (3) The hydrochloric acid modified protein glue solution obtained in step (3) was homogenized (the pressure was set to 50 MPa and the number of treatments was 6 times), treated with high-pressure microfluidization (the pressure was 200 MPa and the number of treatments was 8 times) and spray dried (the inlet air temperature was 180°C and the outlet air temperature was 90°C) to obtain an acid-resistant fish protein glue with a viscosity of 2.2 mpa.s.

[0042] Comparative Example 2 A method for preparing an acid-resistant fish protein glue specifically comprises the following steps: (1) Weigh 6.67 g of fish protein gelatin and add 93.33 g of distilled water, and place in a 60°C water bath for 3 h to obtain a fish protein gelatin solution; (2) adding 1 mol / L hydrochloric acid to the protein glue solution obtained in step (1) to adjust the pH of the fish protein glue solution to 2.8, thereby obtaining a hydrochloric acid-modified protein glue solution; (3) The hydrochloric acid modified protein glue solution obtained in step (3) was homogenized (the pressure was set to 50 MPa and the number of treatments was 6 times), treated with high-pressure microfluidization (the pressure was 200 MPa and the number of treatments was 8 times) and spray dried (the inlet air temperature was 180°C and the outlet air temperature was 90°C) to obtain an acid-resistant fish protein glue with a viscosity of 2.4 mpa.s.

[0043] Comparative Example 3 A method for preparing an acid-resistant fish protein glue specifically comprises the following steps: (1) Weigh 6.67 g of fish protein gelatin and add 93.33 g of distilled water, and place in a 60°C water bath for 3 h to obtain a fish protein gelatin solution; (2) adding 1 mol / L hydrochloric acid to the protein glue solution obtained in step (1) to adjust the pH of the fish protein glue solution to 3.2, thereby obtaining a hydrochloric acid-modified protein glue solution; (3) The hydrochloric acid modified protein glue solution obtained in step (3) was homogenized (the pressure was set to 50 MPa and the number of treatments was 6 times), treated with high-pressure microfluidization (the pressure was 200 MPa and the number of treatments was 8 times) and spray dried (the inlet air temperature was 180°C and the outlet air temperature was 90°C) to obtain an acid-resistant fish protein glue with a viscosity of 2.4 mpa.s.

[0044] Comparative Example 4 A method for preparing an acid-resistant fish protein glue specifically comprises the following steps: (1) Weigh 6.67 g of fish protein gelatin and add 93.33 g of distilled water and place in a 60°C water bath for 3 h to obtain a fish protein gelatin solution; (2) adding 1 mol / L hydrochloric acid to the protein glue solution obtained in step (1) to adjust the pH of the fish protein glue solution to 4.5, thereby obtaining a hydrochloric acid-modified protein glue solution; (3) The hydrochloric acid modified protein glue solution obtained in step (3) was subjected to homogenization treatment (pressure set to 50 MPa, treatment times 6 times), high-pressure microfluidization treatment (pressure set to 200 MPa, treatment times 8 times) and spray drying treatment (inlet air temperature of 180°C, outlet air temperature of 90°C) to obtain an acid-resistant fish protein glue with a viscosity of 2.3 mpa.s.

[0045] Comparative Example 5 A method for preparing an acid-resistant fish protein glue specifically comprises the following steps: (1) Weigh 6.67 g of fish protein gelatin and add 93.33 g of distilled water, and place in a 60°C water bath for 3 h to obtain a fish protein gelatin solution; (2) adding 1 mol / L sodium hydroxide to the protein glue solution obtained in step (1) to adjust the pH of the fish protein glue solution to 5.3, thereby obtaining a hydrochloric acid-modified protein glue solution; (3) The hydrochloric acid modified protein glue solution obtained in step (3) was homogenized (the pressure was set to 50 MPa and the number of treatments was 6 times), treated with high-pressure microfluidization (the pressure was 200 MPa and the number of treatments was 8 times) and spray dried (the inlet air temperature was 180°C and the outlet air temperature was 90°C) to obtain an acid-resistant fish protein glue with a viscosity of 2.5 mpa.s.

[0046] Comparative Example 6 A method for preparing an acid-resistant fish protein glue specifically comprises the following steps: (1) Weigh 6.67 g of fish protein gelatin and add 93.33 g of distilled water, and place in a 60°C water bath for 3 h to obtain a fish protein gelatin solution; (2) adding 1 mol / L sodium hydroxide to the protein glue solution obtained in step (1) to adjust the pH of the fish protein glue solution to 6.3, thereby obtaining a hydrochloric acid-modified protein glue solution; (3) The hydrochloric acid modified protein glue solution obtained in step (3) was homogenized (the pressure was set to 50 MPa and the number of treatments was 6 times), treated with high-pressure microfluidization (the pressure was 200 MPa and the number of treatments was 8 times) and spray dried (the inlet air temperature was 180°C and the outlet air temperature was 90°C) to obtain an acid-resistant fish protein glue with a viscosity of 2.2 mpa.s.

[0047] Comparative Example 7 A method for preparing an acid-resistant fish protein glue specifically comprises the following steps: (1) Weigh 6.67 g of fish protein gelatin and add 93.33 g of distilled water, and place in a 60°C water bath for 3 h to obtain a fish protein gelatin solution; (2) adding 1 mol / L sodium hydroxide to the protein glue solution obtained in step (1) to adjust the pH of the fish protein glue solution to 5.3, thereby obtaining a hydrochloric acid-modified protein glue solution; (3) The hydrochloric acid modified protein glue solution obtained in step (3) was spray dried (the inlet air temperature was 180°C and the outlet air temperature was 90°C) to obtain an acid-resistant fish protein glue with a viscosity of 2.2 mPa.s.

[0048] Example 8 The acid-resistant fish protein glues prepared in Examples 1 to 7 and Comparative Examples 1 to 7 were subjected to gel strength tests, and the specific process was as follows: The gel strength of fish protein gel was tested using a texture analyzer. The treated fish protein gel solution was poured into a 25 mL glass cup and incubated at 4 °C for 16-17 h. The gel strength of the colloid was tested using a P0.5R probe with a diameter of 1.27 cm. The speed before, during and after the test was 1 mm / s. The maximum force applied when the probe entered the colloid (diameter 33 mm, height 22 mm) to a depth of 4 mm was defined as the gel strength.

[0049] The detection structure is shown in Table 1.

[0050] Table 1 Gel strength It can be seen from the above table that locust bean gum can significantly improve the gel strength of fish protein glue under acidic conditions, and homogenization treatment is conducive to the combination of the two to achieve better improvement.

[0051] At the same time, a correlation equation was established based on the gel strength and pH of the acid-resistant fish protein glue prepared in Examples 1 to 7 above, such as Figure 1 As shown, the correlation equation is y=-38.397x2 +370.68x-172.89.

[0052] The acid-resistant fish protein glues prepared in Examples 1 to 7 and Comparative Examples 1 to 7 were tested for melting temperatures, and the specific process was as follows: Pour the treated fish protein glue mixed solution into a 10×100 mm (diameter×length) test tube with a screw cap, leaving some space at the top of the test tube, cover the screw cap, and invert it in a 4 ℃ refrigerator for incubation for 16-17 hours. Place the test tube in a constant temperature water bath at 10 ℃ for 10 minutes. The constant temperature water bath is gradually heated at 0.5 ℃ / min, and the temperature at which the bubbles at the bottom of the test tube begin to move upward is recorded as the melting temperature of the glue.

[0053] The detection structure is shown in Table 2.

[0054] Table 2 Melting temperature It can be seen from Table 2 that locust bean gum can significantly increase the melting temperature of fish protein glue under acidic conditions, and homogenization treatment is conducive to the combination of the two to achieve a better improvement effect.

[0055] The embodiments of the present application are described above in conjunction with the accompanying drawings. Specific examples are used herein to illustrate the principles and implementation methods of the present application. The description of the above embodiments is only used to help understand the core idea of ​​the present application, but the present application is not limited to the above-mentioned specific implementation methods. The above-mentioned specific implementation methods are merely illustrative and not restrictive. Under the inspiration of the present application, ordinary technicians in this field can also make many forms without departing from the scope of protection of the purpose of the present application and the claims, all of which are within the protection of the present application.

Claims

1. A method for preparing an acid-resistant fish protein glue, characterized in that: The following steps are involved: Mixing fish protein glue and locust bean gum and dissolving them in water to obtain a fish protein glue-locust bean gum mixed solution; The pH of the fish protein glue-locust bean gum mixed solution is adjusted to 2-7 by acid or alkali to obtain a mixed solution; The mixed solution is subjected to homogenization treatment, high-pressure microfluidization treatment and drying treatment in sequence to obtain the acid-resistant fish protein glue.

2. The preparation method according to claim 1, characterized in that: The mass ratio of the fish protein glue to the locust bean gum is 10:0.1 to 5:

1.

3. The preparation method according to claim 1, characterized in that: The acid includes a hydrochloric acid solution, and the concentration of the hydrochloric acid solution is 0.5 mol / L to 2 mol / L; The alkali includes a sodium hydroxide solution, and the concentration of the sodium hydroxide solution is 0.5 mol / L to 2 mol / L.

4. The preparation method according to claim 1, characterized in that: The processing conditions during the homogenization process are: pressure 10MPa to 50MPa, and the number of processing times 1 to 8 times; The treatment conditions during the high-pressure microfluidic treatment are: pressure of 50 MPa to 250 MPa, and treatment times of 1 to 9 times.

5. The preparation method according to claim 1, characterized in that: The drying process adopts spray drying process; The treatment conditions during the spray drying process are: inlet air temperature 175°C~185°C, outlet air temperature 80°C~100°C.

6. The preparation method according to claim 1, characterized in that: After the step of mixing the fish protein glue and the locust bean gum and dissolving them in water, the preparation method further comprises: After the fish protein glue and locust bean gum are mixed and dissolved in water, they are placed in a water bath at 50° C. to 80° C. and heated for 1 h to 2 h.

7. An acid-resistant fish protein glue, characterized in that: The invention is prepared by the preparation method according to any one of claims 1 to 6.

8. The acid-resistant fish protein glue according to claim 7, characterized in that The viscosity of the acid-resistant fish protein glue is 2 mpa.s to 5.5 mpa.s.

9. The acid-resistant fish protein glue according to claim 7, characterized in that The gel strength y of the acid-resistant fish protein glue and the pH value x of the mixed solution satisfy the following formula: <h2 style=";text-align:left;direction:ltr">y=-38.397x<h2 style=";text-align:left;direction:ltr"> 2 <h2 style=";text-align:left;direction:ltr"> +370.68x-172.

89.

10. Use of the acid-resistant fish protein glue according to any one of claims 7 to 9 in the preparation of acidic food.

Images