Sample analyzer and electrolyte measurement module

Abstract

The embodiment of the invention provides a sample analyzer and an electrolyte measurement module, the electrolyte measurement module comprises a dilution container, an electrode assembly, a switcher, a first flow path, a second flow path and a liquid driving device, and the first flow path is communicated with the dilution container; the switcher can be switched between a first working position and a second working position, when the switcher is in the first working position, the liquid driving device is communicated with the first flow path through the switcher, and the liquid driving device is disconnected from the second flow path; at the second working position, the switcher enables the liquid driving device to be communicated with the second flow path, and the liquid driving device is disconnected from the first flow path; and when the switcher is switched from the second working position to the first working position, the reflux volume does not exceed 3% of the volume of the dilution liquid required by the dilution container in a single time. According to the embodiment of the invention, the proportion of the reflux volume V1 relative to the volume of the liquid required to be diluted at a time is small, the degree of influence on the dilution accuracy is within an allowable error range, and the dilution accuracy can be improved at least to a certain extent.

Description

Technical Field

[0001] The present application relates to the field of biochemical analysis technology, and in particular to a sample analyzer and an electrolyte measurement module. Background Art

[0002] Before the ISE (ion specific electrode) test, a certain amount of diluent and a certain amount of sample need to be injected into the dilution container, and the sample is diluted to a fixed multiple with the diluent before the sample ion concentration is measured. This measurement method has high requirements for the accuracy of sample dilution. In related technologies, the accuracy of sample dilution fluctuates greatly, thus affecting the test results. Summary of the invention

[0003] In view of this, the embodiments of the present application are intended to provide a sample analyzer and an electrolyte measurement module capable of improving the accuracy of sample dilution.

[0004] The present application embodiment provides a sample analyzer, comprising:

[0005] A sample supply mechanism, used for carrying a sample to be measured;

[0006] A reagent supply mechanism, used for carrying a reagent bottle containing a reagent;

[0007] A first sample dispensing mechanism, used for adding the first sample in the sample supply mechanism into the reaction cup;

[0008] A reagent dispensing mechanism, used for adding the reagent into the reaction cup;

[0009] a detection device, used for performing optical detection on the reaction solution containing the first sample and the reagent in the reaction cup to obtain an optical detection result;

[0010] a second sample dispensing mechanism;

[0011] Electrolyte measurement module, including:

[0012] A dilution container, an electrode assembly, a switch, a first flow path, a second flow path, a measuring device and a liquid driving device, wherein the second sample dispensing mechanism is used to add the second sample in the sample supply mechanism to the dilution container, the dilution container is used to carry the second sample and the diluent, the dilution container has a liquid outlet, the electrode assembly has a liquid flow channel, the liquid flow channel is connected to the liquid outlet, the liquid in the dilution container flows into the liquid flow channel through the liquid outlet, and contacts with the sensitive membranes of each electrode of the electrode assembly to generate an electrode signal, and the measuring device is used to obtain the ion concentration detection result of the second sample according to the electrode signal, wherein the first flow path is connected to the dilution container;

[0013] The switch can be switched between a first working position and a second working position. In the first working position, the switch enables the liquid drive device to connect to the first flow path, and the liquid drive device is disconnected from the second flow path; in the second working position, the switch enables the liquid drive device to connect to the second flow path, and the liquid drive device is disconnected from the first flow path;

[0014] The reflux volume when the switch is switched from the second working position to the first working position does not exceed 3% of the volume of the diluent required for a single time in the dilution container.

[0015] The present application embodiment provides an electrolyte measurement module, including:

[0016] A dilution container, an electrode assembly, a switch, a first flow path, a second flow path, a measuring device and a liquid driving device, wherein the second sample dispensing mechanism is used to add the second sample in the sample supply mechanism to the dilution container, the dilution container is used to carry the second sample and the diluent, the dilution container has a liquid outlet, the electrode assembly has a liquid flow channel, the liquid flow channel is connected to the liquid outlet, the liquid in the dilution container flows into the liquid flow channel through the liquid outlet, and contacts with the sensitive membranes of each electrode of the electrode assembly to generate an electrode signal, and the measuring device is used to obtain the ion concentration detection result of the second sample according to the electrode signal, wherein the first flow path is connected to the dilution container;

[0017] The switch can be switched between a first working position and a second working position. In the first working position, the switch enables the liquid drive device to connect to the first flow path, and the liquid drive device is disconnected from the second flow path; in the second working position, the switch enables the liquid drive device to connect to the second flow path, and the liquid drive device is disconnected from the first flow path;

[0018] The reflux volume when the switch is switched from the second working position to the first working position does not exceed 3% of the volume of the diluent required for a single time in the dilution container.

[0019] In the sample analyzer and electrolytic electrolyte measurement module of the embodiment of the present application, since V1≤V2*0.03, when the switch is switched from the second working position to the first working position, the proportion of the reflux volume V1 relative to the volume of the diluent required for a single time is small, and the degree of influence on the dilution accuracy is within the allowable error range. Therefore, the above-mentioned association design of the reflux container of the switch and the volume of the diluent required for a single time can at least improve the dilution accuracy to a certain extent. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 A simplified schematic diagram of a sample analyzer according to an embodiment of the present application;

[0021] Figure 2 A simplified schematic diagram of an electrolyte measurement module according to an embodiment of the present application;

[0022] Figure 3 This is a simplified schematic diagram of an electrolyte measurement module according to another embodiment of the present application.

[0023] Description of Reference Numerals

[0024] Sample unit 11; first sample dispensing mechanism 12; reagent unit 13; reagent dispensing mechanism 14; mixing mechanism 15; reaction component 16; detection device 17;

[0025] Electrolyte measurement module 2; dilution container 21; waste liquid injector 22; dilution liquid injector 23; storage container 24; first flow path 251A, 251B; second flow path 252A, 252B; three-way valve 26A, 26B; two-way valve 26C, 26D; electrode assembly 27 DETAILED DESCRIPTION

[0026] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0027] The various specific technical features described in the specific embodiments can be combined in any suitable manner without contradiction, for example, different embodiments and technical solutions can be formed by combining different specific technical features. In order to avoid unnecessary repetition, various possible combinations of the specific technical features in the present invention will not be described separately.

[0028] In the following description, the terms "first\second\..." are only used to distinguish different objects, and do not mean that the objects have the same or related points. It should be understood that the directions "above", "below", "outside" and "inside" are all directions in normal use, and the directions "left" and "right" refer to the left and right directions shown in the specific corresponding schematic diagrams, which may be the left and right directions in normal use or not.

[0029] It should be noted that the terms "comprises", "includes" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also includes other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, an element defined by the sentence "comprises a ..." does not exclude the presence of other identical elements in the process, method, article or device including the element. "Multiple" means greater than or equal to two.

[0030] The present application embodiment provides a sample analyzer. Figure 1 , including a sample supply mechanism 11, a reagent supply mechanism 13, a first sample dispensing mechanism 12, a second sample dispensing mechanism, a reagent dispensing mechanism 14, a reaction disk 16, a detection device 17, etc.

[0031] The sample supply mechanism 11 is used to carry the sample to be measured. The sample supply mechanism 11 can be a sample tray, which includes a plurality of sample positions such as sample tubes. The sample tray can dispatch the sample to a corresponding position by rotating its tray structure, for example, a position for the sample dispensing mechanism 12 to absorb the sample. Of course, the sample supply mechanism 11 can also be in other shapes.

[0032] The reagent supply mechanism 13 is used to carry a reagent bottle containing a reagent. In some embodiments, the reagent supply mechanism 13 can be a reagent disk, which is arranged in a disc-shaped structure and has a plurality of positions for carrying reagent bottles. The reagent supply mechanism 13 can rotate and drive the reagent bottle it carries to rotate, and is used to rotate the reagent bottle to a specific position, such as a position where the reagent is sucked by the reagent dispensing mechanism 14.

[0033] The first sample dispensing mechanism 12 is used to add the first sample in the sample supply mechanism 11 to the reaction cup. Exemplarily, the first sample dispensing mechanism 12 includes a moving part and a sample needle disposed on the moving part; the moving part is used to drive the sample needle to move between different operating positions to absorb or discharge the first sample. For example, the sample needle moves to absorb the first sample carried by the sample supply mechanism 11, moves to the reaction cup to be added, and discharges the first sample into the reaction cup.

[0034] The reagent dispensing mechanism 14 is used to add reagents to the reaction cup. For example, the reagent dispensing mechanism 14 is configured with a reagent needle, which moves to absorb the reagent in the reagent bottle carried by the reagent supply mechanism 13, moves to the reaction cup to be added with the reagent, and discharges the reagent into the reaction cup.

[0035] The detection device 17 is used to perform optical detection on the reaction solution containing the first sample and the reagent in the reaction cup to obtain optical detection results, for example, performing optical measurement analysis, calculating the concentration of the component to be detected in the sample through a calibration curve, etc.

[0036] It should be noted that the optical measurement analysis may be immunoassay, biochemical analysis, coagulation analysis, blood rheology analysis, and the like.

[0037] Exemplarily, in some embodiments, the sample analyzer further comprises a mixing mechanism 15, which is used to mix the reaction liquid in the reaction cup. Exemplarily, the mixing mechanism 15 is equipped with a stirring rod, which is used to mix the reaction liquid in the reaction cup.

[0038] See also Figure 2 and Figure 3 The electrolyte measurement module 2 includes a dilution container 21, an electrode assembly 27, a switch, first flow paths 251A and 251B, second flow paths 252A and 252B, a measuring device, and a liquid driving device.

[0039] The second sample dispensing mechanism is used to add the second sample in the sample supply mechanism 11 to the dilution container 21 . The dilution container 21 is used to carry the second sample and the diluent. That is, the dilution container 21 needs to be injected with the dilution liquid and the second sample.

[0040] The specific type of the second sample is not limited, and can be body fluids such as serum, blood, plasma, spinal fluid, urine, gastric juice, intestinal fluid, bile, saliva, tears, or solutions from a living organism such as cell extracts, or can be solutions used in medical treatment such as dialysate, infusion, nutrients, and medicines.

[0041] It should be noted that the first sample dispensing mechanism 12 and the second sample dispensing mechanism may be the same dispensing mechanism or different dispensing mechanisms.

[0042] For example, in some embodiments, the dilution container 21 is located on the motion track of the first sample dispensing mechanism 12 , and the first sample dispensing mechanism 12 can dispense the first sample into the reaction cup and dispense the second sample into the dilution container 21 .

[0043] The dilution container 21 has a liquid outlet, and the electrode assembly 27 has a liquid flow channel, which is connected to the liquid outlet. The liquid in the dilution container 21 (the liquid at least includes the second sample and the diluent) flows into the liquid flow channel through the liquid outlet and contacts the sensitive membranes of each electrode of the electrode assembly 27 to generate an electrode signal.

[0044] The measuring device is used to obtain the ion concentration detection result of the second sample according to the electrode signal. For example, the electrode assembly 27 is used to measure the Li +、Na + , K + , Cl - The plasma concentration is detected.

[0045] Exemplarily, the electrode assembly 27 includes a reference electrode box and multiple ion selective electrode boxes. The output end of each electrode of the electrode assembly 27 is electrically connected to a measuring device. The measuring device measures the potential difference between the electrode in each ion selective electrode box and the electrode in the reference electrode box to obtain the corresponding ion concentration.

[0046] For example, all ion selective electrode cartridges include sodium ion selective electrode cartridges, potassium ion selective electrode cartridges, and chloride ion selective electrode cartridges.

[0047] A flow path is formed in each of the sodium ion selective electrode box, the potassium ion selective electrode box, the chloride ion selective electrode box and the reference electrode box. The flow paths of the respective electrode boxes are connected in sequence to form the above-mentioned liquid flow channel.

[0048] The structures of the above-mentioned electrode boxes may be basically the same, except that the components of the sensitive membranes of the electrodes in the electrode boxes are different.

[0049] The first flow paths 251A and 251B communicate with the dilution container 21 .

[0050] The switch can switch between the first working position and the second working position.

[0051] In the first working position, the switch connects the liquid driving device to the first flow paths 251A and 251B, and disconnects the liquid driving device from the second flow paths 252A and 252B.

[0052] In the second working position, the switch connects the liquid driving device to the second flow paths 252A and 252B, and disconnects the liquid driving device from the first flow paths 251A and 251B.

[0053] It should be noted that the electrolyte measurement module 2 needs to dilute the sample with a diluent to a fixed multiple before measuring the ion concentration. This measurement method can also be called an indirect ion selective electrode method. The indirect ion selective electrode method has very high requirements for the accuracy of sample dilution. If there are bubbles in the dilution during the dilution process, it will affect the final dilution accuracy and thus affect the test results. In addition, if bubbles happen to flow through the electrode during electrode signal measurement, it will also interfere with the electrode signal, thereby affecting the test results.

[0054] The following is a brief introduction to the diluent injection process.

[0055] A steel needle can be used to inject the diluent into the dilution container 21, and the liquid level submerges the tip of the steel needle. The position of the steel needle is then kept unchanged, and the diluent is sucked back through the steel needle until the liquid level drops to the tip of the steel needle and the steel needle can no longer suck back the diluent, thereby ensuring the accuracy of the volume of the diluent.

[0056] In the related art, one source of bubbles in the dilution container is that the diluent itself carries bubbles before being injected into the dilution container. Therefore, a degassing membrane and other measures are provided on the path of injecting the dilution to solve the problem of bubbles carried by the dilution, and other means are also used to prevent the sample from carrying bubbles. However, even so, bubbles will still be generated in the dilution container in some cases. For a long period of time, the industry has no idea how the bubbles in the dilution container are generated, and there is no way to know what means to solve the bubble problem. The accuracy of sample dilution fluctuates greatly, thus affecting the test results.

[0057] The inventor of the present application has finally learned two other sources of bubbles through a large number of experiments. The first one: when the three-way valve in the discharge pipeline is switched, due to the large volumetric effect of the three-way valve, part of the gas in the discharge pipeline will be reversely discharged into the dilution container when the valve core of the three-way valve is switched. If the dilution container is injected with dilution liquid at this time, bubbles will be formed in the dilution liquid. The second one: at the end of the dilution liquid, the dilution liquid is injected into the dilution container through a steel needle. After the dilution liquid is injected, droplets are easily suspended at the needle tip of the steel needle. When the three-way valve switches the working position, due to the large volumetric effect of the three-way valve, the suspended droplets are easily pushed to the dilution container 2. The droplets will not only cause the volume of the dilution liquid in the dilution container to increase, but also may introduce bubbles in the dripping process.

[0058] With the bubble generation principle known, in the embodiment of the present application, the reflux volume V1 when the switch is switched from the second working position to the first working position does not exceed 3% of the dilution volume V2 required for a single time in the dilution container 21, that is, V1≤V2*0.03. For example, when the dilution volume required for a single time in the dilution container 21 is 600 μL (microliters), the reflux volume V2 when the switch is switched from the second working position to the first working position does not exceed 18 μL.

[0059] The volume V2 of dilution liquid required for a single use of the dilution container 21 is basically fixed and does not change with the sample type, that is, the nominal volume of the sample analyzer. For example, the volume V2 of dilution liquid required for a single use of this type of product may be clearly stated in the product manual of the sample analyzer or on the sample analyzer product.

[0060] In the sample analyzer of the embodiment of the present application, since V1≤V2*0.03, when the switch is switched from the second working position to the first working position, the proportion of the reflux volume V1 relative to the volume of the diluent required for a single time is small, and the degree of influence on the dilution accuracy is within the allowable error range. Therefore, the above-mentioned association design of the reflux container of the switch and the volume of the diluent required for a single time can at least improve the dilution accuracy to a certain extent.

[0061] It should be noted that, for a certain switch, its reflux volume is basically unchangeable. Therefore, when designing a sample analyzer, a switch with a suitable reflux volume needs to be designed for a single required diluent volume.

[0062] It should be noted that, on the electrolyte measurement module 2, the reflux volume of the switch can be measured in the following manner: Place the switch in the second working position, ensure that the first flow paths 251A, 251B and the switch are filled with liquid, switch the switch from the second working position to the first working position, and measure the volume of the liquid discharged from the first flow paths 251A, 251B. The volume of the liquid is the reflux volume of the switch.

[0063] The first sample and the second sample may be samples in the same sample container, that is, the first sample and the second sample have the same source and the same type, for example, both are from the blood of the same subject. The first sample and the second sample may also be samples in different sample containers. In this embodiment, the types of the first sample and the second sample may be the same or different, for example, the first sample is blood and the second sample is gastric juice; or, the first sample and the second sample are both blood, and the first sample and the second sample may come from the same subject or from different subjects.

[0064] Exemplarily, the first sample and the second sample are from the same subject. In this way, it is convenient for the sample analyzer to record the information of the same subject. It should be noted that even if the first sample and the second sample are from the same subject, the types of the first sample and the second sample can be the same or different. For example, the first sample and the second sample are blood and urine of the same subject.

[0065] Preferably, the reflux volume V1 when the switch is switched from the second working position to the first working position does not exceed 2% of the dilution volume V2 required for a single time of the dilution container 21. That is, V1≤V2*0.02. In this embodiment, the ratio of the reflux volume V1 to the dilution volume V2 required for a single time of the dilution container 21 when the switch is switched from the second working position to the first working position can be further reduced, which can further improve the bubble phenomenon.

[0066] More preferably, the reflux volume when the switch is switched from the second working position to the first working position does not exceed 1% of the volume of the dilution liquid required for a single time in the dilution container 21, that is, V1≤V2*0.01. In this embodiment, the ratio of the reflux volume V1 when the switch is switched from the second working position to the first working position relative to the volume V2 of the dilution liquid required for a single time in the dilution container 21 can be further improved, which can further improve the bubble phenomenon.

[0067] Exemplarily, the reflux volume V1 when the switch is switched from the second working position to the first working position does not exceed 8.5 μL, and the dilution volume V2 required for a single time in the dilution container 21 is 400 μL to 850 μL. In this embodiment, the reflux volume V1 when the switch is switched from the second working position to the first working position, and the dilution volume V2 required for a single time in the dilution container 21 not only need to satisfy: V1 ≤ 8.5 μL, 400 μL ≤ V2 ≤ 850 μL, but also need to satisfy V1 ≤ V2 * 0.03. In this embodiment, the dilution volume V2 required for a single time in the dilution container 21 is in a reasonable range, which can not only dilute the second sample to a certain multiple well, but also have a reasonable volume, improve operational reliability, and reduce errors.

[0068] Preferably, the volume of diluent required for a single use in the dilution container 21 does not exceed 760 mL, that is, 400 μL≤V2≤760 μL.

[0069] The structural form of the switch is not limited.

[0070] Exemplarily, the switch is a three-way valve 26A, 26B, which has a first working port, a second working port and a third working port, wherein the first working port is connected to the first flow path 251A, 251B, the second working port is connected to the second flow path 252A, 252B, and the third working port is connected to the liquid drive device. The valve core of the three-way valve 26A, 26B can selectively block the first working port or the second working port.

[0071] In the first working position, the valve cores of the three-way valves 26A and 26B block the second working port and connect the first working port and the third working port. In the first working position, the first flow paths 251A and 251B are connected to the liquid drive device, and the space in the liquid drive device is disconnected from the second flow paths 252A and 252B, so that the liquid in the liquid drive device will not enter the second flow paths 252A and 252B.

[0072] In the second working position, the valve cores of the three-way valves 26A and 26B block the first working port, and connect the second working port and the third working port. In the second working position, the second flow paths 252A and 252B are connected to the liquid driving device, and the liquid in the liquid driving device enters the second flow paths 252A and 252B, while the first flow paths 251A and 251B are disconnected from the space in the liquid driving device, and the liquid in the first flow paths 251A and 251B cannot enter the liquid driving device.

[0073] The specific types of the three-way valves 26A and 26B are not limited, for example, they can be manual valves or solenoid valves.

[0074] For example, the three-way valves 26A and 26B are two-position three-way solenoid valves, which have only two working positions, namely the first working position and the second working position mentioned above, so that the valve core structure can be simplified, making the structure of the three-way valves 26A and 26B relatively simple. In addition, by controlling the power on and off of the solenoid valves, the valve core switching of the three-way valves 26A and 26B is controlled to achieve automatic and accurate control.

[0075] Specifically, the two-position three-way solenoid valve includes a valve seat, a valve core, an electromagnet, and an elastic member. The valve seat has a valve cavity. The first working port, the second working port, and the third working port are respectively connected to the valve cavity, and the valve core is slidably arranged in the valve cavity. The elastic member and the electromagnet are used to drive the valve core to reciprocate between the first working position and the second working position. Specifically, when the electromagnet is not energized, the elastic member maintains the valve core in the default position, that is, the initial position. When the electromagnet is energized, the electromagnet and the valve core generate a magnetic attraction force to attract the valve core to move, so that the valve core switches the working position. When the electromagnet is powered off again, the electromagnet no longer generates a magnetic attraction force on the valve core, and the valve core is reset to the initial position under the action of the elastic member.

[0076] It should be noted that the initial position may be the first working position mentioned above, or may be the second working position mentioned above.

[0077] In some preferred embodiments, the initial position is the second working position, that is, when the electromagnet is not powered, the three-way valves 26A and 26B default to disconnecting the first flow paths 251A and 251B from the liquid drive device. In this way, when the ion concentration detection is not performed for a long time, the solenoid valve does not need to be powered, which can avoid the phenomenon of severe heating of the solenoid valve due to long-term power supply. In this embodiment, the switch is switched from the second working position to the first working position, and the corresponding electromagnet is switched from the power-off state to the power-on state.

[0078] In some other embodiments, please refer to Figure 3The switch includes two two-way valves 26C and 26D, wherein one two-way valve 26C connects the first flow path 251A and the liquid driving device, and the other two-way valve 26D connects the second flow path 252A and the liquid driving device.

[0079] When the switch is in the first working position, one two-way valve 26C connected to the first flow path 251A is opened, and the other two-way valve 26D is closed, that is, one two-way valve 26D connected to the second flow path 252A is closed. When the switch is in the second working position, one two-way valve 26C connected to the first flow path 251A is closed, and the other two-way valve 26D is opened, that is, one two-way valve 26D connected to the second flow path 252A is opened. The switch is switched between the first working position and the second working position by coordinated control of the actions of the two two-way valves 26C and 26D.

[0080] The specific type of the liquid drive device is not limited.

[0081] For example, the liquid driving device is a syringe, and the liquid in the first flow path 251A, 251B enters the syringe through the injection port, and then pumps the liquid to the second flow path 252A, 252B through the same injection port. The structure is simple and the cost is low.

[0082] For example, see Figure 2 and Figure 3 The liquid driving device is a waste liquid syringe 22, the liquid flow channel has a waste liquid outlet, one end of the first flow path 251A is connected to the waste liquid outlet, and the other end is connected to the waste liquid syringe 22 through a switch. One end of the second flow path 252A is connected to the waste liquid syringe 22 through a switch, and the waste liquid syringe 22 is used to provide negative pressure to the first flow path 251A and positive pressure to the second flow path 252A, so that the liquid in the dilution container 21 flows into the waste liquid syringe 22 through the liquid flow channel and the first flow path 251A under the action of negative pressure, and the liquid entering the waste liquid syringe 22 is discharged through the second flow path 252A under the action of positive pressure.

[0083] When the switch is in the first working position, the switch connects the waste liquid injector 22 to the first flow path 251A, and disconnects the waste liquid injector 22 from the second flow path 252A. Specifically, in this state, the waste liquid injector 22 provides a negative pressure driving force for the first flow path 251A, and part of the liquid in the dilution container 21 enters the waste liquid injector 22 through the liquid outlet, the liquid flow channel, and the first flow path 251A under the action of the negative pressure driving force.

[0084] When the switch is in the second working position, the switch connects the waste liquid injector 22 to the second flow path 252A, and disconnects the waste liquid injector 22 from the first flow path 251A. Specifically, in this state, the waste liquid injector 22 provides a positive pressure driving force for the second flow path 252A, and almost all the liquid in the waste liquid injector 22 is pumped out through the second flow path 252A under the action of the positive pressure driving force.

[0085] After the liquid in the waste liquid syringe 22 is pumped out, the switch switches from the second working position to the first working position, and part of the liquid in the dilution container 21 is sucked into the waste liquid syringe 22 again. Thereafter, the switch switches from the first working position to the second working position again to discharge the liquid, and the cycle continues until the end.

[0086] Exemplarily, the sample analyzer includes a waste liquid collection container, which is connected to the second flow paths 252A and 252B and is used to collect liquid discharged from the second flow paths 252A and 252B. In this embodiment, the waste liquid is collected by the waste liquid collection container, and the operator can regularly check and dump the waste liquid in the waste liquid collection container in time.

[0087] For some examples, see Figure 2 and Figure 3 , the liquid driving device is a diluent injector 23, the electrolyte measurement module 2 includes a storage container 24 for storing the diluent, and the diluent to be used is contained in the storage container 24. One end of the first flow path 251B is connected to the diluent injector 23 through a switch, and the other end of the first flow path 251B is connected to the diluent container 21. For example, a steel needle is provided at one end of the first flow path 251B, and the diluent is injected into the diluent container 21 through the steel needle. One end of the second flow path 252B is connected to the storage container 24, and the other end is connected to the diluent injector 23 through a switch.

[0088] The diluent injector 23 is used to alternately provide positive pressure to the first flow path 251B and negative pressure to the second flow path 252B, so that the diluent in the storage container 24 flows into the diluent injector 23 through the second flow path 252B under the action of negative pressure, and the liquid entering the diluent injector 23 is injected into the dilution container 21 through the first flow path 251A under the action of positive pressure.

[0089] In this embodiment, the reflux volume when the switch switches from the second working position to the first working position accounts for a relatively small proportion. Therefore, after the injection of the diluent is completed, the reflux volume during the switching process of the switch is small, and it is not easy to push the droplet at the tip of the steel needle into the dilution container 21.

[0090] The present application embodiment provides an electrolyte measurement module 2, comprising:

[0091] The dilution container 21, the electrode assembly 27, the three-way valves 26A and 26B, the first flow paths 251A and 251B, the second flow paths 252A and 252B, the measuring device, and the liquid driving device.

[0092] The dilution container 21 is used to carry samples and diluents. The dilution container 21 has a liquid outlet. The electrode assembly 27 has a liquid flow channel, which is connected to the liquid outlet. The liquid in the dilution container 21 flows into the liquid flow channel through the liquid outlet and contacts the sensitive membranes of each electrode of the electrode assembly 27 to generate electrode signals.

[0093] The first flow paths 251A and 251B communicate with the dilution container 21 .

[0094] The measuring device is used to obtain the ion concentration detection result of the sample according to the electrode signal.

[0095] The three-way valves 26A and 26B can switch between a first working position and a second working position. In the first working position, the three-way valves 26A and 26B connect the liquid drive device to the first flow paths 251A and 251B, and the liquid drive device is disconnected from the second flow paths 252A and 252B; in the second working position, the three-way valves 26A and 26B connect the liquid drive device to the second flow paths 252A and 252B, and the liquid drive device is disconnected from the first flow paths 251A and 251B.

[0096] The reflux volume V1 when the three-way valves 26A, 26B are switched from the second working position to the first working position does not exceed 3% of the volume V2 of the dilution liquid required for a single time in the dilution container 21, that is, V1≤V2*0.03.

[0097] In the electrolyte measurement module 2 of the embodiment of the present application, since V1≤V2*0.03, when the switch is switched from the second working position to the first working position, the proportion of the reflux volume V1 relative to the volume of the diluent required for a single time is small, and the degree of influence on the dilution accuracy is within the allowable error range. Therefore, the above-mentioned association design of the reflux container of the switch and the volume of the diluent required for a single time can at least improve the dilution accuracy to a certain extent.

[0098] Exemplarily, the reflux volume when the three-way valve 26A, 26B is switched from the second working position to the first working position does not exceed 1% of the dilution volume required for a single time in the dilution container 21. In this embodiment, the ratio of the reflux volume V1 to the dilution volume V2 required for a single time in the dilution container 21 when the switch is further switched from the second working position to the first working position can be further improved to further improve the bubble phenomenon.

[0099] The structure of the electrolyte measurement module 2 in the embodiment of the present application may refer to the structure of the electrolyte measurement module 2 in any of the above embodiments, and will not be described in detail here.

[0100] In the description of the present application, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the embodiments of the present application. In the present application, the schematic representation of the above terms is not necessarily for the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine different embodiments or examples described in the present application and the features of different embodiments or examples without contradiction.

[0101] The above description is only a preferred embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A sample analyzer, characterized in that: include: A sample supply mechanism, used for carrying a sample to be measured; A reagent supply mechanism, used for carrying a reagent bottle containing a reagent; A first sample dispensing mechanism, used for adding the first sample in the sample supply mechanism into the reaction cup; A reagent dispensing mechanism, used for adding the reagent into the reaction cup; a detection device, used for performing optical detection on the reaction solution containing the first sample and the reagent in the reaction cup to obtain an optical detection result; a second sample dispensing mechanism; Electrolyte measurement module, including: A dilution container, an electrode assembly, a switch, a first flow path, a second flow path, a measuring device and a liquid driving device, wherein the second sample dispensing mechanism is used to add the second sample in the sample supply mechanism to the dilution container, the dilution container is used to carry the second sample and the diluent, the dilution container has a liquid outlet, the electrode assembly has a liquid flow channel, the liquid flow channel is connected to the liquid outlet, the liquid in the dilution container flows into the liquid flow channel through the liquid outlet, and contacts with the sensitive membranes of each electrode of the electrode assembly to generate an electrode signal, and the measuring device is used to obtain the ion concentration detection result of the second sample according to the electrode signal, wherein the first flow path is connected to the dilution container; The switch can be switched between a first working position and a second working position. In the first working position, the switch enables the liquid drive device to connect to the first flow path, and the liquid drive device is disconnected from the second flow path; in the second working position, the switch enables the liquid drive device to connect to the second flow path, and the liquid drive device is disconnected from the first flow path; The reflux volume when the switch is switched from the second working position to the first working position does not exceed 3% of the volume of the diluent required for a single time in the dilution container.

2. The sample analyzer according to claim 1, characterized in that: The first sample and the second sample are from the same subject.

3. The sample analyzer according to claim 1, characterized in that: The reflux volume of the switch when switching from the second working position to the first working position does not exceed 2% of the volume of the diluent required for a single time in the dilution container.

4. The sample analyzer according to claim 1, characterized in that: The reflux volume of the switch when switching from the second working position to the first working position does not exceed 1% of the volume of the diluent required for a single time in the dilution container.

5. The sample analyzer according to claim 1, characterized in that: The reflux volume of the switch when switching from the second working position to the first working position does not exceed 8.5 μL, and the volume of diluent required for a single dilution container is 400 μL to 850 μL.

6. The sample analyzer according to claim 5, characterized in that: The volume of diluent required for a single use in the dilution container does not exceed 760 μL.

7. The sample analyzer according to any one of claims 1 to 6, characterized in that: The switch is a three-way valve, and the three-way valve has a first working port, a second working port and a third working port, the first working port is connected to the first flow path, the second working port is connected to the second flow path, and the third working port is connected to the liquid driving device; In the first working position, the valve core of the three-way valve blocks the second working port and connects the first working port and the third working port; in the second working position, the valve core of the three-way valve blocks the first working port and connects the second working port and the third working port.

8. The sample analyzer according to claim 7, characterized in that: The three-way valve is a two-position three-way solenoid valve, including a valve seat, a valve core, an electromagnet, and an elastic member. The valve seat has a valve cavity. The first working port, the second working port, and the third working port are respectively connected to the valve cavity. The valve core is slidably arranged in the valve cavity. The elastic member and the electromagnet are used to drive the valve core to reciprocate between the first working position and the second working position.

9. The sample analyzer according to any one of claims 1 to 6, characterized in that: The switch includes two two-way valves, one of which connects the first flow path and the liquid driving device, and the other of which connects the second flow path and the liquid driving device; In the first working position, one of the two-way valves connected to the first flow path is opened, and the other two-way valve is closed; in the second working position, one of the two-way valves connected to the first flow path is closed, and the other two-way valve is opened.

10. The sample analyzer according to any one of claims 1 to 6, characterized in that: The liquid driving device is a syringe.

11. The sample analyzer according to any one of claims 1 to 6, characterized in that: The liquid driving device is a waste liquid syringe, the liquid flow channel has a waste liquid outlet, one end of the first flow path is connected to the waste liquid outlet, and the other end is connected to the waste liquid syringe through the switch, one end of the second flow path is connected to the waste liquid syringe through the switch, and the waste liquid syringe is used to provide negative pressure to the first flow path and positive pressure to the second flow path, so that the liquid in the dilution container flows into the waste liquid syringe through the liquid flow channel and the first flow path under the action of the negative pressure, and the liquid entering the waste liquid syringe is discharged through the second flow path under the action of the positive pressure.

12. The sample analyzer according to claim 11, characterized in that: The sample analyzer comprises a waste liquid collection container, which is in communication with the second flow path and is used for collecting liquid discharged from the second flow path.

13. The sample analyzer according to any one of claims 1 to 6, characterized in that: The liquid driving device is a diluent syringe, the electrolyte measurement module includes a storage container for storing the diluent, one end of the first flow path is connected to the diluent syringe through the switch, one end of the second flow path is connected to the storage container, and the other end is connected to the diluent syringe through the switch; The diluent syringe is used to provide positive pressure to the first flow path and negative pressure to the second flow path, so that the diluent in the storage container flows into the diluent syringe through the second flow path under the action of the negative pressure, and the liquid entering the diluent syringe is injected into the dilution container through the first flow path under the action of the positive pressure.

14. An electrolyte measurement module, characterized in that: include: A dilution container, an electrode assembly, a three-way valve, a first flow path, a second flow path, a measuring device and a liquid driving device, wherein the dilution container is used to carry a sample and a diluent, the dilution container has a liquid outlet, the electrode assembly has a liquid flow channel, the liquid flow channel is communicated with the liquid outlet, the liquid in the dilution container flows into the liquid flow channel through the liquid outlet, and contacts with the sensitive membranes of each electrode of the electrode assembly to generate an electrode signal, and the measuring device is used to obtain an ion concentration detection result of the sample according to the electrode signal, wherein the first flow path is communicated with the dilution container; The three-way valve can be switched between a first working position and a second working position. In the first working position, the three-way valve enables the liquid driving device to be connected to the first flow path, and the liquid driving device is disconnected from the second flow path; In the second working position, the three-way valve enables the liquid driving device to be connected to the second flow path, and the liquid driving device is disconnected from the first flow path; The reflux volume of the three-way valve when switching from the second working position to the first working position does not exceed 3% of the volume of the dilution liquid required for a single time in the dilution container.

15. The electrolyte measurement module according to claim 14, characterized in that: The reflux volume of the three-way valve when switching from the second working position to the first working position does not exceed 1% of the volume of the dilution liquid required for a single time in the dilution container.

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