Oral composition and method for suppressing bitter taste from ergothioneine or salt thereof

Abstract

The purpose of the present invention is to provide: an oral composition containing ergothioneine or a salt thereof in which the bitter taste derived from ergothioneine or a salt thereof is reduced; and a method for suppressing the bitter taste derived from ergothioneine or a salt thereof in an oral composition containing ergothioneine or a salt thereof. The present invention pertains to an oral composition characterized by containing the following components (A) and (B): (A) ergothioneine or a salt thereof, and (B) a pig-derived collagen having a weight-average molecular weight of more than 1000 and 10000 or less.

Description

Technical Field

[0001] The present invention relates to an oral composition containing ergothioneine or a salt thereof and porcine collagen having a weight average molecular weight of greater than 1000 and less than 10000. In addition, the present invention relates to a method for suppressing bitterness derived from ergothioneine or a salt thereof. Background Art

[0002] Ergothioneine is one of the hydrophilic sulfur-containing amino acids, and is known to have various physiological activities including antioxidant activity. Regarding the physiological activities of ergothioneine, for example, Patent Document 1 reports an effect of promoting the production of immune response-activating cytokines. Since ergothioneine has various physiological activities, it has attracted much attention as a food, cosmetic or pharmaceutical product in recent years.

[0003] Patent Literature Patent Document 1: Japanese Patent Application Publication No. 2017-218431 Summary of the invention

[0004] As mentioned above, because thioneine possesses useful physiological activity, the food such as the supplement containing thioneine or its salt is useful for maintaining or promoting health. However, thioneine or its salt has unique fragrance, especially has the bitter taste (bitter feeling) of pricking tongue discomfort, and still has room for improvement in making oral compositions.

[0005] The present invention aims to provide an oral composition containing ergothioneine or its salt, wherein the bitterness from ergothioneine or its salt is alleviated. In addition, the present invention aims to provide a method for suppressing the bitterness from ergothioneine or its salt in an oral composition containing ergothioneine or its salt.

[0006] The present inventors have conducted intensive studies to solve the above problems and have found that the bitterness derived from ergothioneine or its salt can be effectively suppressed by combining ergothioneine or its salt with porcine collagen having a weight average molecular weight of more than 1,000 and not more than 10,000.

[0007] The present invention includes the following compositions and the like. [1] An oral composition comprising the following ingredients (A) and (B): (A) ergothioneine or a salt thereof, and (B) Porcine collagen having a weight average molecular weight of greater than 1,000 and not greater than 10,000. [2] The oral composition according to [1] above, wherein the weight ratio of component (B) to component (A) (calculated as ergothioneine), i.e. (B) / (A), is 5 to 700. [3] The oral composition according to [1] or [2] above, which is a food or beverage. [4] A method for suppressing the bitterness from ergothioneine or its salt, which is a method for suppressing the bitterness from ergothioneine or its salt in an oral composition containing (A) ergothioneine or its salt, characterized in that it includes a step of adding (B) porcine collagen having a weight average molecular weight greater than 1000 and less than 10000 to the oral composition. [5] The method according to [4] above, characterized in that the component (B) is formulated in such a way that the weight ratio of the component (B) to the component (A) (calculated as ergothioneine) in the oral composition, i.e., (B) / (A), is 5 to 700.

[0008] According to the present invention, a kind of oral composition containing thioneine or its salt that bitterness from thioneine or its salt is alleviated can be provided.In addition, according to the present invention, a kind of method for suppressing bitterness from thioneine or its salt in the oral composition containing thioneine or its salt can be provided. DETAILED DESCRIPTION

[0009] The oral composition of the present invention (hereinafter also referred to as "the composition of the present invention") contains (A) ergothioneine or a salt thereof and (B) porcine collagen having a weight average molecular weight of more than 1,000 and not more than 10,000.

[0010] Ergothioneine is a kind of sulfur-containing amino acid. In the present invention, ergothioneine is preferably L-ergothioneine. As the salt of ergothioneine, as long as it is a pharmacologically acceptable salt or a salt allowed in a diet, there is no particular limitation, and it may be any of an acidic salt and an alkaline salt. As an acidic salt, for example, inorganic acid salts such as hydrochloride, sulfate, nitrate, and phosphate can be listed; organic acid salts such as acetate, citrate, maleate, malate, oxalate, lactate, succinate, fumarate, and propionate can be listed. As an alkaline salt, for example, alkali metal salts such as sodium salt and potassium salt can be listed; alkaline earth metal salts such as calcium salt and magnesium salt can be listed.

[0011] Ergothioneine or its salt is not subject to any restrictions such as its form or production method. Ergothioneine or its salt may be a chemically synthesized product, or a substance extracted or purified from a natural product. L-ergothioneine is contained in large quantities in Golden / Yellow Oyster mushroom (scientific name: Pleurotuscornucopiae var.citrinopileatus), which belongs to the genus Pleurotus of the family Pleurotaceae. L-ergothioneine is also contained in mushrooms such as Agaricus bisporus (scientific name: Agaricus bisporus), Grey Oyster Mushroom (scientific name: Pleurotus ostreatus), Lentinula edodes (scientific name: Lentinula edodes), Grifola Frondosa (scientific name: Grifola Frondosa), Ganoderma lucidum (scientific name: Ganoderma lucidum), Hericium erinaceus (scientific name: Hericiumerinaceus), Agrocybe aegerita (scientific name: Agrocybe aegerita), Chanterelles (scientific name: Cantharelus cibarius), Boletus (scientific name: Boletus edulis), Morchella esculenta (scientific name: Morchella esculenta), etc. When L-ergothioneine is obtained from natural products, it is preferably extracted from Pleurotus eryngii. Ergothioneine or its salt can also be produced by microbial fermentation. Ergothioneine or its salt may be an isolated substance.

[0012] In the present invention, "collagen" generally refers to collagen protein. There is no particular limitation on the preparation method of collagen as long as it is derived from pigs. The "collagen" in the present invention also includes collagen peptides obtained by hydrolyzing modified collagen such as collagen derived from pigs or gelatin by enzymes, acids, alkalis, etc. Collagen can be used alone or in combination of two or more collagens.

[0013] As the enzyme used for the above-mentioned hydrolysis treatment, any substance that can cut the peptide bond of collagen or gelatin can be used, for example, collagenase, papain, bromelain, actinine, ficin, cathepsin, pepsin, chymosin, trypsin, and enzyme preparations obtained by mixing these enzymes, etc. As the acid, for example, hydrochloric acid, sulfuric acid, nitric acid, etc. can be used. As the base, for example, sodium hydroxide, calcium hydroxide, etc. can be used.

[0014] In the present invention, the aqueous solution of hydrolyzed collagen may be used directly, or a powdered product may be used after drying, etc. In addition, a product obtained by subjecting the aqueous solution to a commonly used purification treatment may be used in the form of an aqueous solution or a powder.

[0015] Commercially available collagen may be used, and collagen having a weight average molecular weight of more than 1000 and not more than 10000 may be used. Examples of commercially available collagen include "SCP-50NB" (manufactured by Nitta Gelatin Co., Ltd.).

[0016] The weight average molecular weight of the collagen used in the present invention is greater than 1000 and less than 10000, preferably 1500 to 9500, and more preferably 2000 to 9000. When the weight average molecular weight is less than 1000, there is a concern that the bitter taste accompanied by the peculiar odor of low molecular weight collagen may become stronger, and when it is greater than 10000, there is a risk of reduced solubility or absorbability.

[0017] In this specification, the weight average molecular weight of collagen refers to the value measured according to the relative molecular mass determination method of fish oligopeptide powder in the Chinese national standard specification (GB specification) GB / T 22729-2008. Among them, the reagents of M, 451 and M, 189 use substitutes. The weight average molecular weight in this method is calculated by measuring cytochrome C (cytochrome, M, 6500), aprotinin (aprotinin, M, 12500), bacitracin (bacitracin, M, 1450), glycine-glycine-tyrosine-arginine (M, 451), and glycine-glycine-glycine (M, 189) with known molecular weights under the same conditions, and then calculating the weight average molecular weight calibration curve based on the logarithmic relationship between the retention time and the relative molecular mass. The so-called weight average molecular weight in the present invention refers to the weight average molecular weight calculated by converting each standard product according to this method.

[0018] The composition of the present invention contains collagen having a weight average molecular weight of greater than 1000 and less than 10000, thereby reducing the bitterness derived from ergothioneine or its salt. Therefore, continuous ingestion of ergothioneine or its salt can be facilitated. In the composition of the present invention, the weight ratio ((B) / (A)) of component (B) to component (A) (calculated as ergothioneine) is not particularly limited and may be 1 to 1000. When the weight ratio of component (B) to component (A) (calculated as ergothioneine) is within the above range, the bitterness from component (A) can be sufficiently suppressed.

[0019] Porcine collagen having a weight average molecular weight of more than 1000 and less than 10000 has a unique odor reminiscent of animal odor. By combining the collagen with ergothioneine or a salt thereof, the odor derived from the collagen can also be suppressed. In the composition of the present invention, the weight ratio ((B) / (A)) of component (B) relative to component (A) (calculated as ergothioneine) is preferably 5 to 700. This can also fully suppress the odor from component (B), making the composition easier to ingest. The above-mentioned weight ratio is more preferably 7 to 400, further preferably 10 to 300, and particularly preferably 10 to 100. In this specification, the amount of thioneine conversion or similar expression thereof, when component (A) is thioneine, refers to its amount. When component (A) is a salt of thioneine, it refers to the value obtained by multiplying the number of moles of the salt by the molecular weight of thioneine.

[0020] The content of ergothioneine or a salt thereof contained in the composition of the present invention is not particularly limited and can be set according to its form and the like. The content of ergothioneine or its salt contained in the composition of the present invention is preferably 0.1% by weight or more, more preferably 0.2% by weight or more, further preferably 0.3% by weight or more, in terms of ergothioneine conversion, and in addition, preferably 50% by weight or less, more preferably 40% by weight or less, further preferably 30% by weight or less, further preferably 20% by weight or less, and particularly preferably 10% by weight or less. In one embodiment, the content of thioneine or its salt contained in the composition of the present invention is preferably 0.1 to 50 weight %, more preferably 0.1 to 40 weight %, further preferably 0.2 to 30 weight %, further preferably 0.2 to 20 weight %, particularly preferably 0.3 to 10 weight %. When the composition of the present invention contains more than two kinds of thioneine or its salt, the content of the above-mentioned thioneine or its salt refers to their total content. The content of thioneine can be measured by high performance liquid chromatography (HPLC).

[0021] The content of the porcine collagen having a weight average molecular weight of more than 1,000 and not more than 10,000 contained in the composition of the present invention is not particularly limited and can be set according to its form and the like. The content of the above-mentioned collagen contained in the composition of the present invention is, for example, preferably 1% by weight or more, more preferably 5% by weight or more, further preferably 10% by weight or more, further preferably 30% by weight or more, particularly preferably 50% by weight or more, and preferably 99.9% by weight or less, more preferably 99.8% by weight or less, and further preferably 99.5% by weight or less. In one embodiment, the content of the above-mentioned collagen contained in the composition of the present invention is preferably 1 to 99.9% by weight, more preferably 10 to 99.8% by weight, and further preferably 50 to 99.5% by weight. The content of the above-mentioned collagen, when containing two or more kinds of collagen, is their total content. The content of collagen can be measured by HPLC.

[0022] The content of ergothioneine or a salt thereof in the composition of the present invention is preferably 1 to 100 mg, more preferably 2 to 50 mg, further preferably 5 to 25 mg, particularly preferably 5 to 20 mg, in terms of ergothioneine per daily intake for adults. The content of the porcine collagen having a weight average molecular weight of more than 1,000 and 10,000 in the composition of the present invention is preferably 500 to 20,000 mg, more preferably 1,000 to 15,000 mg, and even more preferably 3,000 to 10,000 mg per adult daily intake.

[0023] Ergothioneine or its salt and weight average molecular weight are greater than 1000 and are less than 10000 collagen from pigs and are included in natural products or food and drink, and are compounds with edible experience.Therefore from the perspective of safety, it is generally believed that ergothioneine or its salt and weight average molecular weight are greater than 1000 and are less than 10000 collagen from pigs, such as less problems in daily intake.According to the present invention, a kind of safety can be provided, and the bitter taste from ergothioneine or its salt is alleviated and the composition easily taken in.

[0024] As described above, ergothioneine or its salt is known to exert a variety of physiological activities or health functions, for example, it is known to have an antioxidant effect, a brain function improvement effect, an anti-aging effect, an eye disease improvement effect, a whitening effect, an ultraviolet absorption effect, a melanin production inhibitory effect, an active oxygen elimination effect, an elastase activity inhibitory effect, a wrinkle formation inhibitory effect, a skin relaxation inhibitory effect, an autophagy promoting effect, etc. Therefore, the composition of the present invention can be suitably used for anti-oxidation, brain function improvement, anti-aging, eye disease, whitening, ultraviolet absorption, melanin production inhibition, elimination of active oxygen, elastase activity inhibition, wrinkle formation inhibition, skin sagging inhibition, autophagy promotion, etc.

[0025] Collagen is known to have various health functions such as improving skin moisturizing properties, elasticity, and other skin beautifying effects, and improving blood fluidity. The composition of the present invention can also be used to obtain the above effects exerted by collagen by containing porcine collagen having a weight average molecular weight of greater than 1,000 and less than 10,000.

[0026] The composition of the present invention can be used for either therapeutic use (medical use) or non-therapeutic use (non-medical use). The term "non-therapeutic" refers to a concept that does not include medical behavior, that is, human surgery, treatment or diagnosis.

[0027] The composition of the present invention can be in the form of food and beverage, medicine, quasi-drug, feed, etc. The composition of the present invention can also be a raw material or preparation used by being formulated into food and beverage, medicine, quasi-drug, feed, etc.

[0028] The composition of the present invention is an oral composition. Specific examples of the oral composition include foods and drinks, oral pharmaceuticals, quasi-drugs, and feeds. Foods and drinks or oral pharmaceuticals are preferred, and foods and drinks are more preferred.

[0029] As long as the effect of the present invention is not impaired, the composition of the present invention can also contain any additive, any component on the basis of thioneine or its salt and weight-average molecular weight greater than 1000 and the collagen from pig below 10000. These additives and components can be selected according to the form of the composition, and the material that can be generally used for oral compositions such as food and drink, pharmaceuticals, quasi-drugs, and feeds can be used. When the composition of the present invention is made into food and drink, pharmaceuticals, quasi-drugs, and feeds, its manufacture method is not particularly limited and can be manufactured by a general method. In one embodiment, the composition of the present invention can be a material composed of thioneine or its salt and weight-average molecular weight greater than 1000 and the collagen from pig below 10000. The form of the oral composition of the present invention is not particularly limited, and may be solid (powder, granule, tablet, etc.), liquid, paste, or the like.

[0030] For example, when the composition of the present invention is made into food and drink, thioneine or its salt and weight-average molecular weight can be greater than 1000 and be less than 10000 from the collagen of pig, the composition (for example, food raw material, food additive used as required, etc.) that can be used for food and drink is allocated, and various food and drink are made. Food and drink are not particularly limited, for example, can be enumerated: general food and drink, health food, health supplement food, health drink, functional label food, specific health food, patient food and drink etc.. Above-mentioned health food, health supplement food, functional label food, specific health food etc., for example, can be used in the various preparation forms of granules, tablets, granules, powders, capsules, chewing agents, dry syrups, syrups, liquids, beverages, drinks, liquid food etc.

[0031] When the composition of the present invention is made into pharmaceuticals or quasi-medical products, for example, thioneine or its salt and weight-average molecular weight can be greater than 1000 and be less than 10000 in the collagen from pig, the carrier allowed in pharmacology, the additive added as needed, etc. are prepared, and the pharmaceuticals or quasi-medical products of various dosage forms are made. Such carriers, additives, etc., as long as they can be used for pharmaceuticals or quasi-medical products, can be enumerated as long as the pharmacologically allowed material of pharmaceuticals or quasi-medical products: one or more than two kinds of excipients, binding agents, disintegrants, lubricants, antioxidants, coloring agents, etc. As the dosage form for oral administration of pharmaceuticals or quasi-medical products, can be enumerated: liquid, tablet, powder, fine granules, granules, sugar-coated tablets, capsules, suspensions, emulsions, chewing agents, etc.

[0032] When the composition of the present invention is made into feed, ergothioneine or its salt and collagen from pigs having a weight average molecular weight greater than 1000 and less than 10000 are mixed into the feed. Feed additives are also included in the feed. As feed, for example, feed for livestock such as cattle, pigs, chickens, sheep, horses, etc.; feed for small animals such as rabbits, rats, mice, etc.; pet food for dogs, cats, birds, etc., etc.

[0033] The subject to which the composition of the present invention is ingested or administered (may also be referred to as the subject to be administered) is not particularly limited, but is preferably a human or a non-human mammal, and more preferably a human.

[0034] The present invention also includes the following methods. A method for suppressing bitterness from ergothioneine or its salt, which is a method for suppressing bitterness from ergothioneine or its salt in an oral composition containing (A) ergothioneine or its salt, characterized in that it comprises a step of preparing (B) collagen from pig having a weight average molecular weight greater than 1000 and less than 10000 into the oral composition.

[0035] By the above method, the bitter taste derived from ergothioneine or a salt thereof can be effectively suppressed. In the above method, ergothioneine or a salt thereof, and porcine collagen having a weight average molecular weight of greater than 1,000 and less than 10,000, and preferred embodiments thereof are the same as those of the above-mentioned composition of the present invention.

[0036] In the above method, the blending ratio of component (B) to component (A) (calculated as ergothioneine) is not particularly limited, and a preferred range is the same as the weight ratio of component (B) to component (A) (calculated as ergothioneine) in the composition of the present invention. Among them, when the component (B) is formulated so that the weight ratio ((B) / (A)) of the component (B) to the component (A) (calculated as ergothioneine) in the oral composition is 5 to 700, both the bitterness from the component (A) and the odor from the component (B) can be suppressed, which is preferred.

[0037] According to the above method, bitterness derived from ergothioneine or a salt thereof can be suppressed, and it can also be used in combination with other bitterness suppression methods. Other methods for suppressing bitterness include, for example, a method of adding a sweetener or a flavoring agent to a bitter ingredient to prepare a formulation; a method of coating the bitter ingredient with a sugar coating to prepare a formulation; or a method of encapsulating the bitter ingredient to prepare a formulation.

[0038] In this specification, the numerical range represented by the lower limit and the upper limit, i.e., "lower limit - upper limit", includes the lower limit and the upper limit. For example, the range represented by "1 to 2" means 1 or more and 2 or less, including 1 and 2. In this specification, the upper limit and the lower limit can be set as a range of any combination. Example

[0039] The present invention will be described in more detail below based on examples. However, the present invention is not limited to these examples.

[0040] The materials used in the following evaluation tests are as follows. (A) Ergothioneine: Raw material containing 100% L-ergothioneine (B) Collagen: Raw material containing 100% of porcine collagen with a weight average molecular weight of 5,000

[0041] The weight average molecular weight of collagen was determined according to the relative molecular mass determination method for fish oligopeptide powder in the Chinese national standard specification (GB specification) GB / T22729-2008. Among them, the reagents of M,451 and M,189 used glycine-glycine-tyrosine-arginine (M,451) and glycine-glycine-glycine (M,189) respectively.

[0042] <Example 1: Flavor evaluation test of an oral composition containing ergothioneine and collagen> An oral composition containing ergothioneine and collagen was prepared, and sensory evaluation was performed by three panelists skilled in sensory evaluation. The above (A) and (B) in the amounts shown in Table 1 were mixed to prepare compositions of samples 1 to 6 (oral compositions containing ergothioneine and collagen). The weight ratio ((B) / (A)) of (B) collagen to (A) ergothioneine in each composition is shown in Table 1. Each panelist placed the total amount of the prepared oral composition (for example, 20 mg in total of 10 mg of ergothioneine and 10 mg of collagen in Sample 1) directly on the tongue, and evaluated the bitterness intensity derived from ergothioneine and the odor intensity derived from collagen according to the following criteria.

[0043] (Evaluation criteria for bitterness from ergothioneine) Evaluation was performed with 1 to 5 points (5 levels) according to the following criteria: As the standard score, the bitterness intensity derived from ergothioneine felt when the raw material (A) (10 mg) was placed on the tongue was set to 5 points (control group 1). The evaluations of the three panelists were summarized, and the average values ​​(points) are shown in Table 1. 5 points: Strong bitterness from ergothioneine 4 points: Feel the bitter taste from ergothioneine 3 points: Slightly bitter taste from ergothioneine 2 points: The bitter taste from ergothioneine is not noticeable 1 point: No bitter taste from ergothioneine

[0044] (Evaluation criteria for odor from collagen) Evaluation was performed on a 1 to 5-point scale (5 levels) basis according to the following criteria: As the standard score, the bitterness intensity derived from collagen felt when the raw material (B) (10 mg) was placed on the tongue was set to 5 points (control group 2). The evaluations of the three panelists were summarized, and the average values ​​(points) are shown in Table 1. 5 points: Strong odor from collagen 4 points: Smell of collagen 3 points: Slightly smells the collagen 2 points: The odor from collagen is not noticeable 1 point: No odor from collagen is detected

[0045] [Table 1]

[0046] <Results> It was confirmed that the bitter taste derived from ergothioneine was suppressed by combining ergothioneine and collagen. Regarding collagen, it was also confirmed that when collagen alone was contained, the odor was strong and difficult to ingest, but the degree of the odor was alleviated by combining with an appropriate amount of ergothioneine.

Claims

1. An oral composition, characterized in that Contains the following ingredients (A) and (B): (A) ergothioneine or a salt thereof, and (B) Porcine collagen having a weight average molecular weight of greater than 1,000 and not greater than 10,000.

2. The oral composition according to claim 1, characterized in that The weight ratio of the component (B) to the component (A) (calculated as ergothioneine), that is, (B) / (A) is 5 to 700.

3. The oral composition according to claim 1 or 2, characterized in that It is a food and drink.

4. A method for suppressing bitterness from ergothioneine or its salt, which is a method for suppressing bitterness from ergothioneine or its salt in an oral composition containing (A) ergothioneine or its salt, characterized in that, The method comprises the step of blending (B) porcine collagen having a weight average molecular weight of greater than 1,000 and not greater than 10,000 into the oral composition.

5. The method according to claim 4, characterized in that The component (B) is formulated so that the weight ratio of the component (B) to the component (A) (calculated as ergothioneine) in the oral composition, that is, (B) / (A) is 5 to 700.

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