Nasal mucosa tissue cryopreservation liquid, nasal mucosa tissue preservation kit and application of nasal mucosa tissue cryopreservation liquid in nasal mucosa tissue preservation

By providing nasal mucosal tissue frozen liquid and preservation kit, combined with specific frozen and resuscitation methods, the frozen and resuscitation problems in nasal mucosal tissue preservation are solved, and efficient and safe tissue preservation and high-quality resuscitation of stem cells are achieved.

CN119632023APending Publication Date: 2025-03-18卢明 +1
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Patent Information

Application Number
CN202411822960.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-11
Publication Date
2025-03-18

AI Technical Summary

Technical Problem

The prior art is difficult to effectively preserve nasal mucosal tissue, especially during frozen and resuscitation, which can easily affect the activity and application of stem cells.

Method used

It provides a nasal mucosal tissue frozen solution and nasal mucosal tissue preservation kit. The frozen solution is based on DMEM/F12 and contains components such as human serum albumin, DMSO, dextran 40 glucose injection, glucose sodium chloride injection, vitamin C and Thiazovivin. Through specific frozen and resuscitation methods, ensure efficient and safe storage and resuscitation of nasal mucosal tissue.

Benefits of technology

The safe and efficient freezing and resuscitation of nasal mucosal tissues was achieved. The resuscited stem cells had no significant differences in morphology, cell markers and purity from stem cells obtained in fresh tissue culture, avoiding the pain of multiple tissue collections to the collected persons.

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Abstract

The invention belongs to the technical field of tissue preservation and cell culture, and particularly relates to a nasal mucosa tissue cryopreservation solution, a nasal mucosa tissue preservation kit and application of the nasal mucosa tissue cryopreservation solution in nasal mucosa tissue preservation. The nasal mucosa tissue cryopreservation liquid can be used for safely and efficiently storing the nasal mucosa tissue. The nasal mucosa tissue preservation kit comprises the nasal mucosa tissue cryopreservation liquid and the nasal mucosa tissue resuscitation liquid, and after the nasal mucosa tissue cryopreservation liquid is used for conducting cryopreservation on nasal mucosa tissue, the nasal mucosa tissue resuscitation liquid can conduct safe resuscitation on the cryopreserved nasal mucosa tissue; the stem cells obtained by culturing the resuscitated nasal mucosa tissue and the stem cells obtained by culturing the fresh nasal mucosa tissue have no obvious difference in form, cell markers and purity. And meanwhile, the pain of the collected person caused by collecting the tissue blocks for multiple times is avoided.
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