Application of PDPN positive macrophages in preparation of sepsis treatment medicine

By isolating and studying PDPN-positive peritoneal macrophages regulated by ADAP in a sepsis mouse model, it was found that these cells exhibited M2 polarization phenotype and high phagocytosis activity, which solved the unclear problem of the function of these cells in the prior art and achieved effective application in sepsis treatment drugs.

CN119656192APending Publication Date: 2025-03-21SUZHOU UNIV
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Patent Information

Application Number
CN202411891233.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Priority Date
2024-12-19
Filing Date
2024-12-20
Publication Date
2025-03-21

AI Technical Summary

Technical Problem

At present, the biological characteristics, functions and their specific roles in the pathological process of sepsis are still unclear, and the prior art is difficult to effectively utilize these cells in the preparation of sepsis treatment drugs.

Method used

By constructing a sepsis mouse model, macrophages with high expression of surface PDPN protein were isolated from the abdominal cavity, and the expression of PDPN protein was regulated by ADAP protein. This type of PDPN-positive peritoneal macrophage showed a macrophage M2 polarization phenotype. The expression level of M2-type polarization-related marker genes was verified by RNA-seq, and the phagosomal signaling pathway was significantly enriched in PDPN-positive macrophages.

Benefits of technology

During the host's resistance to bacterial infection with sepsis, PDPN-positive macrophages exhibit high phagocytosis activity and have the potential to prepare sepsis therapeutic drugs.

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Abstract

The invention relates to application of PDPN positive macrophages in preparation of sepsis treatment medicines, and belongs to the technical field of biological medicines. According to the invention, a sepsis mouse model is constructed, macrophages with high PDPN protein expression on the surface are separated from the abdominal cavity, and the expression of the PDPN protein is regulated and controlled by ADAP protein. The PDPN positive peritoneal macrophage shows a macrophage M2 polarization phenotype, RNA-seq verifies that the expression level of M2 type polarization related marker genes is remarkably increased in the PDPN positive macrophage, the PDPN positive peritoneal macrophage has higher phagocytic ability, a phagosome signal channel is remarkably enriched in the PDPN positive macrophage, and the PDPN positive peritoneal macrophage can be used for preparing the PDPN positive peritoneal macrophage. The compound plays a key role in the process that a host resists sepsis bacterial infection, and has the potential of preparing sepsis treatment drugs.
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