Construction method of pig follicular granular cell copper death model

By constructing a copper death model for pig follicle granules, the problem of lack of effective models in the existing technology is solved, the operation process is simplified, and the experimental efficiency is improved, providing a reliable experimental platform for studying the role of copper death in reproductive biology.

CN119685243APending Publication Date: 2025-03-25SICHUAN AGRI UNIV
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Patent Information

Application Number
CN202411927306.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-25
Publication Date
2025-03-25

AI Technical Summary

Technical Problem

The lack of effective copper death model of pig follicle granules in the prior art has affected the study of the causes of follicle development abnormalities.

Method used

The pig ovaries were cleaned and the follicle solution was extracted into complete culture medium, the cell suspension was filtered, and the cell pellet was obtained, and the cells were washed and resuspended. The cells were then treated with silver sulfate solution, the culture medium was discarded and washed, and the copper death model of pig follicle granules was obtained.

Benefits of technology

The operation process was successfully simplified and the copper death model of pig follicle granules was constructed, providing a reliable experimental platform for studying the role of copper death in reproductive biology and improving experimental efficiency.

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Abstract

The invention discloses a construction method of a pig follicular granular cell copper death model, and relates to the technical field of biology. The construction method of the pig follicular granule cell copper death model comprises the following steps: cleaning a pig ovary, extracting follicular fluid into a complete culture medium, filtering cell suspension, centrifuging, cleaning obtained cell precipitate, adding the cell precipitate into the complete culture medium to resuspend cells, culturing, treating with copper sulfate, discarding the culture medium, and cleaning to obtain the pig follicular granule cell copper death model. And obtaining the pig follicular granular cell copper death model. According to the method provided by the invention, the pig follicular granule cell copper death model can be successfully established, the operation process is simplified, and the problem that an effective model is lacked in the prior art is solved.
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