Resuscitation method of cryopreserved cells
By using divalent cations to treat frozen cells during resuscitation, the problem of low survival rate of resuscitation after frozen cells is solved, the proliferation ability and activity of cells is improved, cytokine secretion is promoted, and the efficiency of cell treatment is improved.
CN119709613APending Publication Date: 2025-03-28SHANGHAI JUNCELL THERAPEUTICS CO LTD
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Patent Information
- Application Number
- CN202411937552.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Priority Date
- 2023-12-29
- Filing Date
- 2024-12-26
- Publication Date
- 2025-03-28
AI Technical Summary
Technical Problem
The survival rate of immune cells that resuscitate after frozen storage is low, and it takes a long time to recover to the state before frozen storage, which affects the efficiency of cell therapy.
Method used
Incubate the incubation process by adding divalent cations when frozen cells are resuscited, including thawing in culture medium containing divalent cations for at least 5 hours.
Benefits of technology
It significantly improves the proliferation ability, activity and cytokine secretion level of resuscitation, and shortens cell production time.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention relates to a cryopreserved cell recovery method. The method for resuscitating the cryopreserved cells comprises the following steps: unfreezing the frozen cells, and treating the unfrozen cells with divalent cations. According to the method, the multiplication capacity and activity of the recovered immune cells and the secretion level of cell factors are improved.
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