Method for biologically synthesizing 1, 3-propylene glycol by reducing SAM free radical enzyme through photoenzyme
By reducing GD-AE using the photoenzyme PSP2, the in-situ controllable reduction of SAM radical enzyme was achieved, and the problem of expensive and low conversion rate of coenzyme B12 in biological production of 1,3-propanediol was solved, and 1,3-propanediol synthesis was achieved under high efficiency and mild conditions.
CN119709886APending Publication Date: 2025-03-28TIANJIN UNIV
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Patent Information
- Application Number
- CN202411873577.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-18
- Publication Date
- 2025-03-28
AI Technical Summary
Technical Problem
The existing biological method of producing 1,3-propylene glycol has problems such as expensive coenzyme B12, low product concentration, long production cycle and low glycerol conversion rate.
Method used
By reducing GD-AE affiliated to the SAM radical enzyme family using photoenzyme PSP2, the in-situ controlled reduction of SAM radical enzymes is achieved, thereby constructing a synthesis pathway of 1,3-propylene glycol, avoiding dependence on coenzyme B12.
Benefits of technology
It has achieved efficient biosynthesis of 1,3-propylene glycol, with the advantages of high reaction efficiency and mild reaction conditions.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention discloses a method for biosynthesizing 1, 3-propylene glycol by reducing SAM free radical enzyme through photoenzyme, which comprises the following steps: 1) under anaerobic conditions, putting GD, SAM free radical enzyme, YqhD, PSP2, SAM, glycerol, DTT, NADH and NADPH into a container, adding a Tris-HCl buffer solution, and uniformly mixing; the SAM free radical enzyme is GD-AE; (2) irradiating with light with the wavelength of 405nm, and keeping for 20 minutes at the temperature of 15-30 DEG C under a dark condition; 3) repeating the step 2) twice; and detecting to obtain the 1, 3-propylene glycol. According to the method, the GD-AE belonging to the SAM free radical enzyme family is reduced by using the photoenzyme PSP2, the in-situ controllable reduction of the SAM free radical enzyme is realized, the synthetic route of the 1, 3-propylene glycol is constructed and realized, the participation of coenzyme B12 is not needed, the reaction efficiency is high, and the reaction conditions are mild.
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