A method and application for detecting the GPR107 gene associated with duck egg weight trait

By screening the GPR107 gene molecular marker using whole-genome resequencing technology, and combining it with PCR and Sanger sequencing, the problem of traditional phenotypic selection being unable to improve duck egg weight was solved, enabling rapid and accurate identification of duck egg weight traits and improving breeding efficiency and economic benefits.

CN119710028BActive Publication Date: 2025-10-31JIANGSU INST OF POULTRY SCI
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Patent Information

Application Number
CN202510018066.1
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-01-06
Publication Date
2025-10-31
Estimated Expiration
2045-01-06

AI Technical Summary

Technical Problem

Traditional phenotypic selection is ineffective in improving egg weight traits in ducks, resulting in slow progress in waterfowl breeding. Furthermore, existing technologies lack rapid and accurate gene detection methods, making it difficult to meet the genetic selection requirements for egg weight traits.

Method used

The GPR107 gene molecular marker, which is significantly associated with duck egg weight, was screened using whole-genome resequencing technology. PCR amplification and Sanger sequencing were performed using specific primers to detect the SNP molecular marker genotype at position 7909242 of chromosome 18, providing a rapid and accurate method for identifying duck egg weight traits.

Benefits of technology

This technology enables rapid and accurate identification of duck egg weight traits, improves the accuracy and efficiency of breeding, reduces breeding costs, meets the breeding needs of different markets for egg weight, and improves the breeding efficiency of duck egg weight traits.

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Abstract

This invention relates to a method and application for detecting the GPR107 gene, which is associated with duck egg weight traits, and belongs to the field of biotechnology. This invention detects the genotype of the GPR107 gene at position 7909242 on chromosome 18, determining that individuals with the T / T genotype have higher egg weights than those with the T / C and C / C genotypes. Specifically, individuals with the T / C genotype have higher egg weights than those with the C / C genotype. Using this method to screen for high-quality laying ducks offers the advantages of efficient and rapid identification of duck egg weight traits, improving selection accuracy, accelerating breeding progress, and reducing breeding and production costs. Furthermore, the detection method disclosed in this invention is simple and easy to operate, can be carried out in a laboratory, and can also be applied to genomic breeding technology.
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