Method for assisting in identifying PD-L1 expression of colorectal cancer patient by utilizing patient ascites source PTC (Positive Temperature Coefficient)
PTC tumor microspheres from ascites originated from colorectal cancer patients were constructed through PTC technology, and the PD-L1 expression level was detected by immunohistochemistry, which solved the problem of low accuracy of PD-L1 detection in ascites samples of colorectal cancer patients, and achieved accurate prediction of patient immunotherapy sensitivity.
CN119716060APending Publication Date: 2025-03-28ZHEJIANG CORNERSTONE PRECISION MEDICINE CO LTD
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Patent Information
- Application Number
- CN202311256711.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2023-09-27
- Publication Date
- 2025-03-28
AI Technical Summary
Technical Problem
The prior art is difficult to accurately detect the PD-L1 expression level in ascites samples of colorectal cancer patients, especially in patients who cannot obtain surgical tissue, and the accuracy of PD-L1 detection is low.
Method used
PTC tumor microspheres derived from ascites in colorectal cancer patients were constructed by PTC technology, and the PD-L1 expression level in PTC tumor microspheres was detected by immunohistochemistry.
Benefits of technology
A method to accurately assist in identifying the PD-L1 expression levels of ascites samples of colorectal cancer patients without access to surgical tissue is provided to help predict patient sensitivity to immune checkpoint inhibitor treatment.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention provides a method for auxiliary identification of PD-L1 expression of a colorectal cancer patient by using a patient ascites-derived tumor-like cell cluster (PTC), and a kit for identifying the PD-L1 expression of the colorectal cancer patient by using the PD-L1 expression of the colorectal cancer patient. PTC is a result of self-assembly and proliferation of tumor cells, primary epithelial cells, interstitial cells, fibroblasts and immune cells, an ascites sample can be utilized to be cultured into tumor microspheres within a short time, and the obtained tumor microspheres have high consistency with original tumors. PD-L1 detection can be provided for patients who cannot obtain tissue slices by utilizing the PTC derived from malignant effusion of colorectal cancer patients, and the feasibility of receiving immunotherapy is provided for the patients.
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