Non-targeted metabolomics detection method and its application in multi-matrix samples based on liquid chromatography-mass spectrometry

By adopting specific mobile phases and optimized chromatographic conditions in the LCMS technology, non-targeted metabolomics detection of multi-matrix samples is solved, and a more comprehensive metabolite detection and analysis is achieved.

CN119738504AInactive Publication Date: 2025-04-01BEIJING SANYUAN FOOD
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Patent Information

Application Number
CN202510098541.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-01-22
Publication Date
2025-04-01
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

The existing LCMS methods mainly focus on metabolites analysis of single matrix samples, which cannot fully reflect the metabolic status in the organisms, and there is a lack of research on multi-matrix samples detection, resulting in limited metabolites coverage.

Method used

A non-targeted metabolomics detection method based on the multi-matrix samples used in liquid-mass CT is adopted to achieve high coverage detection of multi-matrix samples by selecting specific mobile phases and optimized chromatographic conditions, combined with mass spectrometry and data analysis technology.

Benefits of technology

It improves the detection coverage and accuracy of metabolites, provides a high-quality database that can fully reflect the metabolic status in the organism and overcomes the limitations of single matrix sample detection.

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Abstract

The present invention discloses a non-targeted metabolomics detection method for multi-matrix samples based on liquid chromatography-mass spectrometry and its application, which belongs to the field of detection and analysis. The detection method comprises the following steps: pre-treating the matrix sample and performing LC-MS / MS positive and negative ion mode analysis, wherein in the LC-MS / MS positive and negative ion mode analysis, the mobile phase A used in the liquid chromatography comprises ammonium acetate and ammonium fluoride. The detection method of the present invention selects a specific mobile phase, has a high detection coverage rate for metabolites, provides a high-quality database for the identification of non-targeted metabolomics metabolites, and the metabolite information must be complete, so that the metabolites can be identified as accurately as possible.
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